Tassazione e costo del lavoro nei paesi industriali

Il lavoro analizza gli effetti sul costo del lavoro determinati dal livello e dalla composizione del prelievo fiscale su tale fattore produttivo. Esso fornisce in particolare una valutazione quantitativa della traslazione dellÂ’imposta sui costi con riferimento a quattordici paesi industriali nel periodo 1980-1996. I risultati dellÂ’analisi econometrica sono i seguenti; (i) il livello e la variazione del prelievo tributario e contributivo hanno effetti statisticamente significativi sul costo del lavoro; (ii) non vi è riscontro per lÂ’ipotesi dellÂ’invarianza dellÂ’incidenza del prelievo; lÂ’imposta sul reddito e i contributi sociali a carico del datore di lavoro hanno unÂ’influenza più marcata sui costi rispetto ai contributi a carico del lavoratore; (iii) la traslazione dellÂ’imposta è più forte in quei paesi europei caratterizzati da un sistema di contrattazione salariale intermedio tra completa decentralizzazione e piena centralizzazione. Alla luce di questi risultati, il lavoro fornisce infine una valutazione dei provvedimenti volti a ridurre il prelievo sul lavoro assunti in Italia negli ultimi anni. Tali provvedimenti mirano ad abbassare le due componenti del prelievo - imposta sul reddito e contributi sociali a carico del datore di lavoro - che esercitano alla luce dellÂ’analisi econometrica un effetto maggiore sul costo del lavoro.

Recensione alla mostra: Roma medievale. Il volto perduto della città (Museo di Roma Pa-lazzo Braschi, 21 ottobre 2022 - 5 febbraio 2023), a cura di Marina Righetti e Anna Maria D’Achille

Recensione alla mostra: Roma medievale. Il volto perduto della città (Museo di Roma Pa-lazzo Braschi, 21 ottobre 2022 - 5 febbraio 2023), a cura di Marina Righetti e Anna Maria D’Achill

Nanotechnology approaches to self-organized bio-molecular devices

Abstract In this paper we briefly describe new strategies to exploit self-assembled solid-state biomolecular materials as active elements of electronic devices. Two basically different approaches are proposed: a top-down approach, where biomolecular semiconductors consisting of DNA basis are self-organized and interconnected by planar metallic nanopatterns, and a bottom-up approach, where single or ordered matalloproteins are immobilized in a nanocircuit realizing a hybrid covalently bound biologic–inorganic system. The transport characteristics of different devices such as diodes, photodetectors and metal–semiconductor–metal structures will be described

Inorganic Nanomaterials versus Polymer-Based Nanoparticles for Overcoming Neurodegeneration

Neurodegenerative disorders (NDs) affect a great number of people worldwide and also have a significant socio-economic impact on the aging population. In this context, nanomedicine applied to neurological disorders provides several biotechnological strategies and nanoformulations that improve life expectancy and the quality of life of patients affected by brain disorders. However, available treatments are limited by the presence of the blood-brain barrier (BBB) and the blood-cerebrospinal fluid barrier (B-CSFB). In this regard, nanotechnological approaches could overcome these obstacles by updating various aspects (e.g., enhanced drug-delivery efficiency and bioavailability, BBB permeation and targeting the brain parenchyma, minimizing side effects). The aim of this review is to carefully explore the key elements of different neurological disorders and summarize the available nanomaterials applied for neurodegeneration therapy looking at several types of nanocarriers. Moreover, nutraceutical-loaded nanoparticles (NPs) and synthesized NPs using green approaches are also discussed underling the need to adopt eco-friendly procedures with a low environmental impact. The proven antioxidant properties related to several natural products provide an interesting starting point for developing efficient and green nanotools useful for neuroprotection

High Doses of Silica Nanoparticles Obtained by Microemulsion and Green Routes Compromise Human Alveolar Cells Morphology and Stiffness Differently

Among all the inorganic nanomaterials used in commercial products, industry, and medicine, the amorphous silica nanoparticles (SiO2 NPs) appeared to be often tolerated in living organisms. However, despite several toxicity studies, some concerns about the exposure to high doses of SiO2 NPs with different sizes were raised. Then, we used the microemulsion method to obtain stable SiO2 NPs having different sizes (110 nm, 50 nm, and 25 nm). In addition, a new one-pot green synthetic route using leaves extract of Laurus nobilis was performed, obtaining monodispersed ultrasmall SiO2 NPs without the use of dangerous chemicals. The NPs achieved by microemulsion were further functionalized with amino groups making the NPs surface positively charged. Then, high doses of SiO2 NPs (1 mg/mL and 3 mg/mL) achieved from the two routes, having different sizes and surface charges, were used to assess their impact on human alveolar cells (A549), being the best cell model mimicking the inhalation route. Cell viability and caspase-3 induction were analyzed as well as the cellular uptake, obtaining that the smallest (25 nm) and positive-charged NPs were more able to induce cytotoxicity, reaching values of about 60% of cell death. Surprisingly, cells incubated with green SiO2 NPs did not show strong toxicity, and 70% of them remained vital. This result was unusual for ultrasmall nanoobjects, generally highly toxic. The actin reorganization, nuclear morphology alteration, and cell membrane elasticity analyses confirmed the trend achieved from the biological assays. The obtained data demonstrate that the increase in cellular softness, i.e., the decrease in Young's modulus, could be associated with the smaller and positive NPs, recording values of about 3 kPa. On the contrary, green NPs triggered a slight decrease of stiffness values (c.a. 6 kPa) compared to the untreated cells (c.a. 8 kPa). As the softer cells were implicated in cancer progression and metastasization, this evidence strongly supported the idea of a link between the cell elasticity and physicochemical properties of NPs that, in turn, influenced the interaction with the cell membrane. Thus, the green SiO2 NPs compromised cells to a lesser extent than the other SiO2 NPs types. In this scenario, the elasticity evaluation could be an interesting tool to understand the toxicity of NPs with the aim of predicting some pathological phenomena associated with their exposure

Conformation of microcontact-printed proteins by atomic force microscopy molecular sizing.

We investigated the structural changes occurring in proteins patterned via microcontact printing. This was done by molecular sizing using atomic force microscopy to observe the structure of printed individual metalloprotein molecules in the unlabeled and untreated states. We observed that the size of the printed proteins were more than 2-fold smaller than the native shape, which indicates that some deformations take place upon the contact-assisted adsorption on silanized silicon dioxide. This can be attributed to simultaneously occurring effects, and particularly to the sandwiching between surfaces of very different hydrophilic/hydrophobic properties during contact lithography

Self-chemisorption of azurin on functionalized oxide surfaces for the implementation of biomolecular devices

Abstract In this work, we investigate the formation of redox protein Azurin (Az) monolayers on functionalized oxygen exposing surfaces. These metallo-proteins mediate electron transfer in the denitrifying chain of Pseudomonas bacteria and exhibit self-assembly properties, therefore they are good candidates for bio-electronic applications. Azurin monolayers are self-assembled onto silane functionalized surfaces and characterized by atomic force microscopy (AFM). We show also that a biomolecular field effect transistor (FET) in the solid state can be implemented by interconnecting an Azurin monolayer immobilized on SiO 2 with two gold nanoelectrodes. Transport experiments, carried out at room temperature and ambient pressure, show FET behavior with conduction modulated by the gate potential