Longitudinal study of the effect of sporidesmin toxicity on lamb production and serum biochemistry in a flock of 46 Romney ewes using a standardised measure of liver damage

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Effect of Palpable Udder Defects on Milk Yield, Somatic Cell Count, and Milk Composition in Non-Dairy Ewes

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Low temperature decreases bone mass in mice: Implications for humans

ObjectivesHumans exhibit significant ecogeographic variation in bone size and shape. However, it is unclear how significantly environmental temperature influences cortical and trabecular bone, making it difficult to recognize adaptation versus acclimatization in past populations. There is some evidence that cold‐induced bone loss results from sympathetic nervous system activation and can be reduced by nonshivering thermogenesis (NST) via uncoupling protein (UCP1) in brown adipose tissue (BAT). Here we test two hypotheses: (1) low temperature induces impaired cortical and trabecular bone acquisition and (2) UCP1, a marker of NST in BAT, increases in proportion to degree of low‐temperature exposure.MethodsWe housed wildtype C57BL/6J male mice in pairs at 26 °C (thermoneutrality), 22 °C (standard), and 20 °C (cool) from 3 weeks to 6 or 12 weeks of age with access to food and water ad libitum (N = 8/group).ResultsCool housed mice ate more but had lower body fat at 20 °C versus 26 °C. Mice at 20 °C had markedly lower distal femur trabecular bone volume fraction, thickness, and connectivity density and lower midshaft femur cortical bone area fraction versus mice at 26 °C (p < .05 for all). UCP1 expression in BAT was inversely related to temperature.DiscussionThese results support the hypothesis that low temperature was detrimental to bone mass acquisition. Nonshivering thermogenesis in brown adipose tissue increased in proportion to low‐temperature exposure but was insufficient to prevent bone loss. These data show that chronic exposure to low temperature impairs bone architecture, suggesting climate may contribute to phenotypic variation in humans and other hominins.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/146428/1/ajpa23684.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/146428/2/ajpa23684_am.pd

Could operational hydrological models be made compatible with satellite soil moisture observations?

Soil moisture is a significant state variable in flood forecasting. Nowadays more and more satellite soil moisture products are available, yet their usage in the operational hydrology is still limited. This is because the soil moisture state variables in most operational hydrological models (mostly conceptual models) are over-simplified—resulting in poor compatibility with the satellite soil moisture observations. A case study is provided to discuss this in more detail, with the adoption of the XAJ model and the Soil Moisture and Ocean Salinity (SMOS) level-3 soil moisture observation to illustrate the relevant issues. It is found that there are three distinct deficiencies existed in the XAJ model that could cause the mismatch issues with the SMOS soil moisture observation: (i) it is based on runoff generation via the field capacity excess mechanism (interestingly, such a runoff mechanism is called the saturation excess in XAJ while in fact it is clearly a misnomer); (ii) evaporation occurs at the potential rate in its upper soil layer until the water storage in the upper layer is exhausted, and then the evapotranspiration process from the lower layers will commence – leading to an abrupt soil water depletion in the upper soil layer; (iii) it uses the multi-bucket concept at each soil layer – hence the model has varied soil layers. Therefore, it is a huge challenge to make an operational hydrological model compatible with the satellite soil moisture data. The paper argues that this is possible and some new ideas have been explored and discussed. Copyright © 2016 John Wiley & Sons, Ltd. Citing Literatur

Ruminant Brucellosis in the Kafr El Sheikh Governorate of the Nile Delta, Egypt: Prevalence of a Neglected Zoonosis

Brucellosis is a zoonosis of mammals caused by bacteria of the genus Brucella. It is responsible for a vast global burden imposed on human health through disability and on animal productivity. In humans brucellosis causes a range of flu-like symptoms and chronic debilitating illness. In livestock brucellosis causes economic losses as a result of abortion, infertility and decreased milk production. The main routes for human infection are consumption of contaminated dairy products and contact with infected ruminants. The control of brucellosis in humans depends on its control in ruminants, for which accurate estimates of the frequency of infection are very useful, especially in areas with no previous frequency estimates. We studied the seroprevalence of brucellosis and its geographic distribution among domestic ruminants in one governorate of the Nile Delta region, Egypt. In the study area, the seroprevalence of ruminant brucellosis is very high and has probably increased considerably since the early 1990s. The disease is widespread but more concentrated around major animal markets. These findings question the efficacy of the control strategy in place and highlight the high infection risk for the animal and human populations of the area and the urgent need for an improved control strategy

Characterization of a compact wideband microwave metasurface lens for cryogenic applications

In this paper, we present characterization of a compact flat microwave lens operating between 6 GHz and 14 GHz using a near field scanning system. An X-band horn antenna and open-end rectangular waveguide were used as an illumination source and probe, respectively. |S21| is measured as the probe antenna moves on a plane orthogonal to the optical axis vertically and horizontally. The lens is made of a metasurface layer that is sandwiched by two layers of cross-oriented gratings. The overall dimension of the lens is 10 cm in diameter and 0.57 cm in thickness. The measurement results show that the lens's focal length is 8 cm, and the beamwidth (full width at half maximum (FWHM)) is 3.5 cm, A transmission efficiency of over 90% and a cross-polarization gain of 25 dB were achieved over the entire bandwidth. The measurement results at room temperature are in good agreement with numerical simulations. The proposed lens will be used in a cryogenic environment e.g. dilution refrigerators for quantum computing systems. More results at cryogenic temperature e.g, below 30 K will be shown at the conference

Wireless microwave signal transmission for cryogenic applications

Microwave wireless signal propagation in cryogenic environments has applications in radio astronomy and quantum computing. This paper demonstrates for the first time a cryogenic wireless setup and investigates the antenna-to-antenna signal transmission in Liquid Nitrogen (LN) and inside the dilution refrigerator at room temperature (296 K). The antenna under investigation consists of a wideband antenna operating in from 8-12 GHz. The antenna was modelled and designed in CST MWS and fabricated on the Rogers RT/duroid 5880 substrate. The measured transmission coefficient (S21) results demonstrate that there was reasonable signal transmission between the antenna pairs when tested in LN (77 K) and inside the dilution refrigerator (tested at 296 K). The results indicate that the proposed Over- The-Air (OTA) system is suitable for cryogenic applications down to 77K

An Intermittent Live Cell Imaging Screen for siRNA Enhancers and Suppressors of a Kinesin-5 Inhibitor

Kinesin-5 (also known as Eg5, KSP and Kif11) is required for assembly of a bipolar mitotic spindle. Small molecule inhibitors of Kinesin-5, developed as potential anti-cancer drugs, arrest cell in mitosis and promote apoptosis of cancer cells. We performed a genome-wide siRNA screen for enhancers and suppressors of a Kinesin-5 inhibitor in human cells to elucidate cellular responses, and thus identify factors that might predict drug sensitivity in cancers. Because the drug's actions play out over several days, we developed an intermittent imaging screen. Live HeLa cells expressing GFP-tagged histone H2B were imaged at 0, 24 and 48 hours after drug addition, and images were analyzed using open-source software that incorporates machine learning. This screen effectively identified siRNAs that caused increased mitotic arrest at low drug concentrations (enhancers), and vice versa (suppressors), and we report siRNAs that caused both effects. We then classified the effect of siRNAs for 15 genes where 3 or 4 out of 4 siRNA oligos tested were suppressors as assessed by time lapse imaging, and by testing for suppression of mitotic arrest in taxol and nocodazole. This identified 4 phenotypic classes of drug suppressors, which included known and novel genes. Our methodology should be applicable to other screens, and the suppressor and enhancer genes we identified may open new lines of research into mitosis and checkpoint biology

A Regional Reduction in Ito and IKACh in the Murine Posterior Left Atrial Myocardium Is Associated with Action Potential Prolongation and Increased Ectopic Activity.

BACKGROUND: The left atrial posterior wall (LAPW) is potentially an important area for the development and maintenance of atrial fibrillation. We assessed whether there are regional electrical differences throughout the murine left atrial myocardium that could underlie regional differences in arrhythmia susceptibility. METHODS: We used high-resolution optical mapping and sharp microelectrode recordings to quantify regional differences in electrical activation and repolarisation within the intact, superfused murine left atrium and quantified regional ion channel mRNA expression by Taqman Low Density Array. We also performed selected cellular electrophysiology experiments to validate regional differences in ion channel function. RESULTS: Spontaneous ectopic activity was observed during sustained 1Hz pacing in 10/19 intact LA and this was abolished following resection of LAPW (0/19 resected LA, P<0.001). The source of the ectopic activity was the LAPW myocardium, distinct from the pulmonary vein sleeve and LAA, determined by optical mapping. Overall, LAPW action potentials (APs) were ca. 40% longer than the LAA and this region displayed more APD heterogeneity. mRNA expression of Kcna4, Kcnj3 and Kcnj5 was lower in the LAPW myocardium than in the LAA. Cardiomyocytes isolated from the LAPW had decreased Ito and a reduced IKACh current density at both positive and negative test potentials. CONCLUSIONS: The murine LAPW myocardium has a different electrical phenotype and ion channel mRNA expression profile compared with other regions of the LA, and this is associated with increased ectopic activity. If similar regional electrical differences are present in the human LA, then the LAPW may be a potential future target for treatment of atrial fibrillation