Enraizamento de brotações epicórmicas de decepa e de envergadura de caule de Vochysia bifalcata Warm. com a aplicação de ácido indolbutírico.

Resumo

How to set up and apply reference levels in fluoroscopy at a national level

A nationwide survey was launched to investigate the use of fluoroscopy and establish national reference levels (RL) for dose-intensive procedures. The 2-year investigation covered five radiology and nine cardiology departments in public hospitals and private clinics, and focused on 12 examination types: 6 diagnostic and 6 interventional. A total of 1,000 examinations was registered. Information including the fluoroscopy time (T), the number of frames (N) and the dose-area product (DAP) was provided. The data set was used to establish the distributions of T, N and the DAP and the associated RL values. The examinations were pooled to improve the statistics. A wide variation in dose and image quality in fixed geometry was observed. As an example, the skin dose rate for abdominal examinations varied in the range of 10 to 45mGy/min for comparable image quality. A wide variability was found for several types of examinations, mainly complex ones. DAP RLs of 210, 125, 80, 240, 440 and 110Gy cm2 were established for lower limb and iliac angiography, cerebral angiography, coronary angiography, biliary drainage and stenting, cerebral embolization and PTCA, respectively. The RL values established are compared to the data published in the literatur

Detrital Neodymium and (Radio)Carbon as Complementary Sedimentary Bedfellows? The Western Arctic Ocean as a Testbed

Interactions between organic and detrital mineral phases strongly influence both the dispersal and accumulation of terrestrial organic carbon (OC) in continental margin sediments. Yet the complex interplay among biological, chemical, and physical processes limits our understanding of how organo-mineral interactions evolve during sediment transfer and burial. In particular, diverse OC sources and complex hydrodynamic processes hinder the assessment of how the partnership of organic matter and its mineral host evolves during supply and dispersal over continental margins. In this study, we integrate new and compiled sedimentological (grain size, surface area), organic (%OC, OC-δ13C, OC-F14C), and inorganic isotopic (εNd, 87Sr/86Sr) geochemical data for a broad suite of surface sediments spanning the Western Arctic Ocean from the Bering Sea to the Mackenzie River Delta that capture diverse sources and ages of both terrestrial and marine material deposited in contrasting shelf and slope settings. Spatial gradients in sediment properties were used to delineate regional sources and transport processes influencing the dispersion and persistence of OC-mineral particle associations during export and burial. We found strong relationships between physical parameters, aluminum content, and OC-14C suggesting that terrestrial OC remains tightly associated with its detrital mineral carrier during source-to-sink transport. Notably, carbon and neodymium isotopic data yield consistent information regarding organic matter provenance. Results obtained highlight the potential for coupled organic-inorganic tracer measurements to elucidate sediment sources and to constrain physical and geochemical processes during sediment mobilization and transport in the Western Arctic Ocean. Tandem measurements of carbon and Nd isotopes may provide a new way to identify large-scale biogeochemical and ecological changes in the sources, nature, and fate of OC stemming from predicted increases in sea ice loss and fluvial inputs of dissolved and particulate OC to this complex and dynamic high latitude marginal sea

Prognostic impact of systemic inflammatory diseases in elderly patients with congestive heart failure

Background and aims: Inflammation is part of the pathophysiology of congestive heart failure (CHF). However, little is known about the impact of the presence of systemic inflammatory disease (SID), defined as inflammatory syndrome with constitutional symptoms and involvement of at least two organs as co-morbidity on the clinical course and prognosis of patients with CHF. Methods and results: This is an analysis of all 622 patients included in TIME-CHF. After an 18 months follow-up, outcomes of patients with and without SID were compared. Primary endpoint was all-cause hospitalization free survival. Secondary endpoints were overall survival and CHF hospitalization free survival. At baseline, 38 patients had history of SID (6.1%). These patients had higher N-terminal pro brain natriuretic peptide and worse renal function than patients without SID. SID was a risk factor for adverse outcome [primary endpoint: hazard ratio (HR) = 1.73 (95% confidence interval: 1.18-2.55, P = 0.005); survival: HR = 2.60 (1.49-4.55, P = 0.001); CHF hospitalization free survival: HR = 2.3 (1.45-3.65, P < 0.001)]. In multivariate models, SID remained the strongest independent risk factor for survival and CHF hospitalization free survival. Conclusions: In elderly patients with CHF, SID is independently accompanied with adverse outcome. Given the increasing prevalence of SID in the elderly population, these findings are clinically important for both risk stratification and patient managemen

GroEL provides a folding pathway with lower apparent activation energy compared to spontaneous refolding of human carbonic anhydrase II

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/117184/1/feb2s0014579397006637.pd

Interactions of Cathinone NPS with Human Transporters and Receptors in Transfected Cells

Pharmacological assays carried out in transfected cells have been very useful for describing the mechanism of action of cathinone new psychoactive substances (NPS). These in vitro characterizations provide fast and reliable information on psychoactive substances soon after they emerge for recreational use. Well-investigated comparator compounds, such as methamphetamine, 3,4-methylenedioxymethamphetamine, cocaine, and lysergic acid diethylamide, should always be included in the characterization to enhance the translation of the in vitro data into clinically useful information. We classified cathinone NPS according to their pharmacology at monoamine transporters and receptors. Cathinone NPS are monoamine uptake inhibitors and most induce transporter-mediated monoamine efflux with weak to no activity at pre- or postsynaptic receptors. Cathinones with a nitrogen-containing pyrrolidine ring emerged as NPS that are extremely potent transporter inhibitors but not monoamine releasers. Cathinones exhibit clinically relevant differences in relative potencies at serotonin vs. dopamine transporters. Additionally, cathinone NPS have more dopaminergic vs. serotonergic properties compared with their non-β-keto amphetamine analogs, suggesting more stimulant and reinforcing properties. In conclusion, in vitro pharmacological assays in heterologous expression systems help to predict the psychoactive and toxicological effects of NPS

A radioimmunoassay for quantifying carbonic anhydrase isozymes in crude lysates

A radioimmunoassay was developed for quantifying each of the two genetically distinct forms of primate carbonic anhydrase, carbonic anhydrases I and II, in unpurified lysates. Under the given experimental conditions, the assay is capable of detecting a minimum of 0.025 μg of carbonic anhydrase I and 0.005 μg of carbonic anhydrase II. There is approximately 98% reproducibility upon repeated assays of a given hemolysate.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/44165/1/10528_2004_Article_BF00485779.pd

Quantitative genetic variation in carbonic anhydrase isozymes from tissues of the pig-tailed macaque, Macaca nemestrina

Two isozymes of carbonic anhydrase (CA I and CA II) were quantified by a radio-immunoassay in 10 different tissues of the pig-tailed macaque. There were clearly differences in relative amounts of the two isozymes, indicating a differential regulation of these two different gene products. An inherited deficiency variant reduced red cell CA I and CA II 5000-fold and 2.7-fold, respectively. In nine other tissues, CA I was reduced from approximately twofold to 110-fold, and CA II was essentially unchanged. The CA I in deficient red cells was immunochemically and electrophoretically identical to common electrophoretic variants of CA I in the pig-tailed macaque and was enzymatically active.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/44174/1/10528_2004_Article_BF00485860.pd