Routine Changing of Intravenous Administration Sets Does Not Reduce Colonization or Infection in Central Venous Catheters

Objective: To determine the effect of routine intravascular administration-set changes on central venous catheter (CVC) colonization and catheter related bacteremia (CRB). Design: Prospective, randomised controlled trial Setting: 18-bed ICU in a University-affiliated, tertiary referral hospital. Participants: 404 chlorhexidine and silver sulfadiazine coated multi-lumen CVCs from 251 intensive care unit (ICU) patients. Interventions: After ethical approval, CVCs inserted in ICU and in situ on Day 4 were randomised to have their administration-sets changed on Day 4 (n = 203) or not at all (n = 201). Fluid container and blood product administration-set use was limited to 24 hours. CVCs were removed (Day 7, not required or suspected infection), and cultured for colonization ( 15 cfu). Medical and laboratory staff were blinded. CRB was diagnosed by a blinded intensivist using strict definitions. Data was collected on; catheter life, CVC site, APACHE II score, patient age, diagnosis, hyperglycemia, hypoalbuminemia, immune status, number of fluid containers and intravenous injections, propofol, blood, TPN or lipid infusion. Results: There were 10 colonized CVCs in the set change group and 19 in the no change group. This was not a statistically significant difference on Kaplan Meier survival analysis (Effect Size = 0.09, Log Rank = 0.87, df = 1, p = 0.35). There were 3 cases of CRB per group. Logistic regression found that burns diagnosis and increased ICU stay were the only factors that significantly predicted colonization (p < 0.001). Conclusions: Intravenous administration-sets can be used for 7-days. Routine administration-set changes are unnecessary before this time

Smith Theory for algebraic varieties

We show how an approach to Smith Theory about group actions on CW-complexes using Bredon cohomology can be adapted to work for algebraic varieties.Comment: Published by Algebraic and Geometric Topology at http://www.maths.warwick.ac.uk/agt/AGTVol4/agt-4-8.abs.htm

Mesolevel networks and the diffusion of social movements: The case of the Swedish Social Democratic Party

In analyzing the spatial diffusion of the Swedish Social Democratic Party, this article introduces the notion of a mesolevel network. A mesolevel network is a social network that differs in three important respects from interpersonal microlevel networks directly linking prior and potential adopters of a practice to one another: (1) it is generated by a different causal process than the microlevel network; (2) it tends to be much sparser than the microlevel network; and (3) the typical edge of a mesolevel network bridges much longer sociometric and geographic distances than the typical edge of a microlevel network. These types of mesolevel networks are important because they can dramatically influence the speed at which a contagious practice will diffuse. The mesolevel network focused upon in this article is the network that emerged out of the travel routes of political agitators affiliated with the Social Democratic Party. Computational modeling shows that the diffusion of the Social Democratic Party is likely to have been considerably influenced by the structure of this network. Empirical analyses of the founding of party organizations during the period 1894-1911 support these theoretical predictions and suggest that this mesolevel network was of considerable importance for the diffusion of the Swedish Social Democratic Party

Inviwo -- A Visualization System with Usage Abstraction Levels

The complexity of today's visualization applications demands specific visualization systems tailored for the development of these applications. Frequently, such systems utilize levels of abstraction to improve the application development process, for instance by providing a data flow network editor. Unfortunately, these abstractions result in several issues, which need to be circumvented through an abstraction-centered system design. Often, a high level of abstraction hides low level details, which makes it difficult to directly access the underlying computing platform, which would be important to achieve an optimal performance. Therefore, we propose a layer structure developed for modern and sustainable visualization systems allowing developers to interact with all contained abstraction levels. We refer to this interaction capabilities as usage abstraction levels, since we target application developers with various levels of experience. We formulate the requirements for such a system, derive the desired architecture, and present how the concepts have been exemplary realized within the Inviwo visualization system. Furthermore, we address several specific challenges that arise during the realization of such a layered architecture, such as communication between different computing platforms, performance centered encapsulation, as well as layer-independent development by supporting cross layer documentation and debugging capabilities

An authorisation framework for actively controlled running gear

A Shift2Rail funded research project called RUN2Rail has investigated a range of new technologies for railway rolling stock. The project included a task on the use of active suspensions, and one of the subtasks was to propose a strategy supporting the authorisation by safety authorities for highly innovative mechatronic vehicles to be placed on the market. The incorporation of electronics and control into suspension systems is still at an early stage, so this paper provides a framework for a practical and efficient authorisation strategy, primarily based upon existing European regulations and standards but in general applicable worldwide

The clinical, radiological, microbiological, and molecular profile of the skin-penetration site of transfemoral amputees treated with bone-anchored prostheses.

The breach of the skin barrier is a critical issue associated with the treatment of individuals with transfemoral amputation (TFA) using osseointegrated, percutaneous titanium implants. Thirty TFA patients scheduled for abutment exchange or removal were consecutively enrolled. The aims were to determine the macroscopic skin signs, the presence of bacteria and the gene expression in abutment-adherent cells and to conduct correlative and comparative analyses between the different parameters. Redness and a granulation ring were present in 47% of the patients. Bacteria were detected in 27/30 patients, commonly in the bone canal. Staphylococcus aureus, coagulase-negative staphylococci, streptococci, and Enterococcus faecalis were the most common. A positive correlation was found between TNF-α expression and the detection of S. aureus. Staphylococcus aureus together with other bacterial species revealed a positive relationship with MMP-8 expression. A negative correlation was demonstrated between the length of the residual femur bone and the detection of a granulation ring and E. faecalis. A positive correlation was revealed between fixture loosening and pain and the radiological detection of endosteal bone resorption. Fixture loosening was also correlated with the reduced expression of interleukin-10 and osteocalcin. It is concluded that several relationships exist between clinical, radiological, microbiological, and molecular assessments of the percutaneous area of TFAs. Further long term studies on larger patient cohorts are required to determine the precise cause-effect relationships and unravel the role of host-bacteria interactions in the skin, bone canal and on the abutment for the longevity of percutaneous implants as treatment of TFA. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 578-589, 2017

Identification of cattle-derived volatiles that modulate the behavioral response of the biting midge culicoides nubeculosus

Identification of host-derived volatiles is an important step towards the development of novel surveillance and control tools for Culicoides biting midges. In this study, we identified compounds from headspace collections of cattle hair and urine that modulate the behavioral response of Culicoides nubeculosus, a research model species with a similar host-range as the vectors of Bluetongue disease and Schmallenberg disease in Europe. Combined gas chromatography and electroantennographic detection (GC-EAD) analysis revealed 23 bioactive compounds, of which 17, together with octanal, were evaluated in a two-choice behavioral assay in the presence of CO2. Decanal, 2-phenylethanal, 1-octen-3-ol, 2-ethylhexanol, 3-methylindole, phenol, and 3-ethylphenol elicited attraction of host seeking C. nubeculosus, whereas heptanal, octanal, nonanal, 3-propylphenol, and 4-propylphenol inhibited the insects’ attraction to CO2, when compared to CO2 alone. 6-Methyl-5-hepten-2-one, 3-methylphenol, 4-methylphenol, and 4-ethylphenol elicited both attraction and inhibition. The behavioral responses were dependent on the concentration tested. Our results show that cattle-derived odors have the potential to be used for the manipulation of the behavior of Culicoides biting midges

Detection of Salmonella spp in animal feed by PCR

The aim of this study was to develop a rapid PCR-based detection method to confirm presumptive isolates of Salmonella spp. as well as to directly detect Salmonella in feed samples. The specificity of the assay was confirmed by testing I 04 Salmonella strains and 43 strains of other Enterobacteriaceae species. A simple sample preparation method was developed based on dilution of suspended cells picked from agar plates after selective enrichment. The method was evaluated on 109 food and feed samples and there was 100% agreement between the traditional method based on biochemical tests and the PCR method. The inhibitory effect on PCR for 15 different feed samples was overcome by using Tth DNA polymerase in combination with addition of glycerol, which enabled direct detection of Salmonella cells in feed