Calculations of three-dimensional hypervelocity cone-flow with chemical reactions

We present the results of CFD calculations of the hypervelocity flow of nitrogen about a slightly blunted cone at an angle of incidence. We use Pullin's Equilibrium Flux Method, and include the effect of finite rate dissociation and recombination chemical reactions vis the Lighthill-Freeman model of the ideal dissociating gas. Test results with the chemical reactions frozen are compared with the perfect gas calculations of Marconi for the same flow geometry and Mach number. The results for chemical reacting flow show substantial interaction between the gas dynamics and the chemistry on the leeward cone surface

Multicenter evaluation of the vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of gram-positive aerobic bacteria

Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF) is gaining momentum as a tool for bacterial identification in the clinical microbiology laboratory. Compared with conventional methods, this technology can more readily and conveniently identify a wide range of organisms. Here, we report the findings from a multicenter study to evaluate the Vitek MS v2.0 system (bioMérieux, Inc.) for the identification of aerobic Gram-positive bacteria. A total of 1,146 unique isolates, representing 13 genera and 42 species, were analyzed, and results were compared to those obtained by nucleic acid sequence-based identification as the reference method. For 1,063 of 1,146 isolates (92.8%), the Vitek MS provided a single identification that was accurate to the species level. For an additional 31 isolates (2.7%), multiple possible identifications were provided, all correct at the genus level. Mixed-genus or single-choice incorrect identifications were provided for 18 isolates (1.6%). Although no identification was obtained for 33 isolates (2.9%), there was no specific bacterial species for which the Vitek MS consistently failed to provide identification. In a subset of 463 isolates representing commonly encountered important pathogens, 95% were accurately identified to the species level and there were no misidentifications. Also, in all but one instance, the Vitek MS correctly differentiated Streptococcus pneumoniae from other viridans group streptococci. The findings demonstrate that the Vitek MS system is highly accurate for the identification of Gram-positive aerobic bacteria in the clinical laboratory setting

Alternating Hemiplegia of Childhood-Related Neural and Behavioural Phenotypes in Na+,K+-ATPase α3 Missense Mutant Mice

Missense mutations in ATP1A3 encoding Na(+),K(+)-ATPase α3 have been identified as the primary cause of alternating hemiplegia of childhood (AHC), a motor disorder with onset typically before the age of 6 months. Affected children tend to be of short stature and can also have epilepsy, ataxia and learning disability. The Na(+),K(+)-ATPase has a well-known role in maintaining electrochemical gradients across cell membranes, but our understanding of how the mutations cause AHC is limited. Myshkin mutant mice carry an amino acid change (I810N) that affects the same position in Na(+),K(+)-ATPase α3 as I810S found in AHC. Using molecular modelling, we show that the Myshkin and AHC mutations display similarly severe structural impacts on Na(+),K(+)-ATPase α3, including upon the K(+) pore and predicted K(+) binding sites. Behavioural analysis of Myshkin mice revealed phenotypic abnormalities similar to symptoms of AHC, including motor dysfunction and cognitive impairment. 2-DG imaging of Myshkin mice identified compromised thalamocortical functioning that includes a deficit in frontal cortex functioning (hypofrontality), directly mirroring that reported in AHC, along with reduced thalamocortical functional connectivity. Our results thus provide validation for missense mutations in Na(+),K(+)-ATPase α3 as a cause of AHC, and highlight Myshkin mice as a starting point for the exploration of disease mechanisms and novel treatments in AHC

Increasing dietary neutral detergent fiber concentration decreases ruminal hydrogen sulfide concentrations in steers fed high-sulfur diets based on ethanol coproducts

Cattle feedlot diets commonly contain ethanol coproducts that are high in S. This dietary S is reduced in the rumen by sulfate reducing bacteria, resulting in an accumulation of hydrogen sulfide (H2S), increasing the risk for S toxicity. A negative correlation between H2S and ruminal pH has been observed previously. The objective of this study was to determine the effect of varying dietary NDF from chopped bromegrass hay (66% NDF) on performance, ruminal pH, and ruminal H2S gas concentration of steers fed a high-S finishing diet. One hundred fifty crossbred steers (359 ± 51 kg BW) were blocked by BW into pens of 5 steers and randomly assigned within block to 1 of 5 treatments (n = 6 pens per treatment) and fed for 84 d. Dietary treatments included 3.5, 5.7, 7.9, 10.1, or 11.4% roughage NDF (rNDF) from bromegrass hay and contained 0.46% dietary S from a combination of dried distillers grains with solubles and condensed corn distillers solubles. In all diets, hay was added at the expense of dry-rolled corn. Effective NDF increased linearly (P \u3c 0.01) with increased inclusion of rNDF. Final BW was not affected by rNDF (P ≥ 0.12). The addition of roughage did not affect ADG (P ≥ 0.13) or gain efficiency (P ≥ 0.12). Dry matter intake increased linearly (P \u3c 0.01) as rNDF concentration increased. There was a treatment × month interaction for S intake (P \u3c 0.01), explained by steers fed 3.5 or 11.4% rNDF increasing S intake each month whereas the middle rNDF inclusions had similar S intake between months 1 and 2 and increased in month 3. Ruminal H2S concentrations and ruminal fluid pH were measured at 6 h postfeeding on d 7, 14, 21, 29, and 84. Ruminal pH increased linearly (P \u3c 0.01; 5.48, 5.61, 5.71, 5.74, and 5.80 ± 0.041 for 3.5, 5.7, 7.9, 10.1, and 11.4% rNDF, respectively) and ruminal H2S concentrations decreased linearly (P \u3c 0.01; 1.00, 0.86, 0.76, 0.70, and 0.62 ± 0.037 g/m3 for 3.5, 5.7, 7.9, 10.1, and 11.4% rNDF, respectively) as rNDF inclusion increased. Using mixed model regression analysis, ruminal pH had a strong negative relationship with ruminal H2S concentrations (β = –0.63; P \u3c 0.01). Under conditions of this study, increasing roughage did not affect cattle gains but helped maintain greater ruminal pH and decreased H2S concentration, suggesting that this dietary strategy may lessen the risk of S toxicity in feedlot cattle