Negative erosion and negative emissions : Combining multiple land-based carbon dioxide removal techniques to rebuild fertile topsoils and enhance food production

Funding Information: This research was supported by the Research Foundation—Flanders (FWO) and by the European Commissions (H2020 FET-open project Super Bio-Accelerated Mineral weathering: A new climate risk hedging reactor technology—“BAM”). JS was supported by Spanish Government Project PID2020115770RB-I. Publisher Copyright: Copyright © 2022 Janssens, Roobroeck, Sardans, Obersteiner, Peñuelas, Richter, Smith, Verbruggen and Vicca.Peer reviewedPublisher PD

Circulating FGF21 in humans is potently induced by short term overfeeding of carbohydrates

Objective: Fibroblast-growth factor 21 (FGF21) is thought to be important in metabolic regulation. Recently, low protein diets have been shown to increase circulating FGF21 levels. However, when energy contribution from dietary protein is lowered, other macronutrients, such as carbohydrates, must be increased to meet eucaloric balance. This raises the possibility that intake of a diet rich in carbohydrates may induce an increase in plasma FGF21 levels per se. Here we studied the role of dietary carbohydrates on the levels of circulating FGF21 and concomitant physiologic effects by feeding healthy men a carbohydrate rich diet without reducing protein intake. Methods: A diet enriched in carbohydrates (80 E% carbohydrate; CHO) and a eucaloric control diet (CON) were provided to nine healthy men for three days. The energy intake during the CHO diet was increased (+75% energy) to ensure similar dietary protein intake in CHO and CON. To control for the effect of caloric surplus, we similarly overfed (+75% energy) the same subjects for three days with a fat-rich diet (78 E% fat; FAT), consisting of primarily unsaturated fatty acids. The three diets were provided in random order. Results: After CHO, plasma FGF21 concentration increased 8-fold compared to CON (329 ± 99 vs. 39 ± 9 pg ml−1, p < 0.05). In contrast, after FAT only a non-significant tendency (p = 0.073) to an increase in plasma FGF21 concentration was found. The increase in FGF21 concentration after CHO correlated closely (r = 0.88, p < 0.01) with increased leg glucose uptake (62%, p < 0.05) and increased hepatic glucose production (17%, p < 0.01), indicating increased glucose turnover. Plasma fatty acid (FA) concentration was decreased by 68% (p < 0.01), supported by reduced subcutaneous adipose tissue HSL Ser660 phosphorylation (p < 0.01) and perilipin 1 protein content (p < 0.01), pointing to a suppression of adipose tissue lipolysis. Concomitantly, a 146% increase in the plasma marker of hepatic de novo lipogenesis C16:1 n−7 FA (p < 0.01) was observed together with 101% increased plasma TG concentration (p < 0.001) in association with CHO intake and increased plasma FGF21 concentration. Conclusion: Excess dietary carbohydrate, but not fat, led to markedly increased FGF21 secretion in humans, notably without protein restriction, and affected glucose and lipid homeostais. Keywords: FGF21, Diet, Carbohydrates, Lipolysis, Live

Exercise Alleviates Lipid-Induced Insulin Resistance in Human Skeletal Muscle–Signaling Interaction at the Level of TBC1 Domain Family Member 4

Excess lipid availability causes insulin resistance. We examined the effect of acute exercise on lipid-induced insulin resistance and TBC1 domain family member 1/4 (TBCD1/4)-related signaling in skeletal muscle. In eight healthy young male subjects, 1 h of one-legged knee-extensor exercise was followed by 7 h of saline or intralipid infusion. During the last 2 h, a hyperinsulinemic-euglycemic clamp was performed. Femoral catheterization and analysis of biopsy specimens enabled measurements of leg substrate balance and muscle signaling. Each subject underwent two experimental trials, differing only by saline or intralipid infusion. Glucose infusion rate and leg glucose uptake was decreased by intralipid. Insulin-stimulated glucose uptake was higher in the prior exercised leg in the saline and the lipid trials. In the lipid trial, prior exercise normalized insulin-stimulated glucose uptake to the level observed in the resting control leg in the saline trial. Insulin increased phosphorylation of TBC1D1/4. Whereas prior exercise enhanced TBC1D4 phosphorylation on all investigated sites compared with the rested leg, intralipid impaired TBC1D4 S341 phosphorylation compared with the control trial. Intralipid enhanced pyruvate dehydrogenase (PDH) phosphorylation and lactate release. Prior exercise led to higher PDH phosphorylation and activation of glycogen synthase compared with resting control. In conclusion, lipid-induced insulin resistance in skeletal muscle was associated with impaired TBC1D4 S341 and elevated PDH phosphorylation. The prophylactic effect of exercise on lipid-induced insulin resistance may involve augmented TBC1D4 signaling and glycogen synthase activation

Microstructural dependence of the fracture toughness of metallic thin films: A bulge test and atomistic simulation study on single-crystalline and polycrystalline silver films

The microstructure contribution to the very low fracture toughness of freestanding metallic thin films was investigated by bulge fracture tests on 200-nm-thick {100} single-crystalline and polycrystalline silver films. The single-crystalline films exhibited a significantly lower fracture toughness value (KIC= 0.88 MPa m1/2) than their polycrystalline counterparts (KIC= 1.45 MPa m1/2), which was rationalized by the observation of an unusual crack initiation behavior—characterized by twinning in front of the notch tip—during in situ testing in the atomic force microscope. Twinning was also observed as a dominant deformation mechanism in atomistic simulations. This twinning tendency is explained by comparing the resolved shear stresses acting on the leading partial dislocation and the full dislocation, which allows to develop a size- and orientation-dependent twinning criterion. The fracture toughness of polycrystalline samples was found to be higher because of the energy dissipation associated with full dislocation plasticity and because of crack meandering along grain boundaries

Lithospheric erosion in the Patagonian slab window, and implications for glacial isostasy

Author Posting. © American Geophysical Union, 2022. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geophysical Research Letters 49(2), (2022): e2021GL096863, https://doi.org/10.1029/2021GL096863.The Patagonian slab window has been proposed to enhance the solid Earth response to ice mass load changes in the overlying Northern and Southern Patagonian Icefields (NPI and SPI, respectively). Here, we present the first regional seismic velocity model covering the entire north-south extent of the slab window. A slow velocity anomaly in the uppermost mantle indicates warm mantle temperature, low viscosity, and possibly partial melt. Low velocities just below the Moho suggest that the lithospheric mantle has been thermally eroded over the youngest part of the slab window. The slowest part of the anomaly is north of 49°S, implying that the NPI and the northern SPI overlie lower viscosity mantle than the southern SPI. This comprehensive seismic mapping of the slab window provides key evidence supporting the previously hypothesized connection between post-Little Ice Age anthropogenic ice mass loss and rapid geodetically observed glacial isostatic uplift (≥4 cm/yr).The facilities of the IRIS Consortium are supported by the National Science Foundation’s SAGE Award under Cooperative Support Agreement EAR-1851048. The GUANACO project is funded by the National Science Foundation under grants EAR-1714154 to WUSTL and EAR-1714662 to SMU, and Erik R. Ivins was supported by NASA under grant NNH19ZDA001N-GRACEFO.2022-07-1

Reliability and realizability risk evaluation of concept designs

This thesis addresses the improvement in quality of decision making in design through the use of decomposed design evaluation. The research reported in this thesis is supported by the Design Research Methodology. To perform decomposed decision making, it is necessary to identify criteria that are deemed important for this activity. Questionnaire surveys, literature review and interviews with industry helped to identify these criteria. Reliability and realizability are two criteria that are selected for research in this thesis. The questionnaire surveys are discussed in chapter 2. A review of literature on decision making, reliability and realizability is reported in chapters 3 and 4. Methodologies for evaluating reliability and physical realizability are discussed in chapter 5. Relative reliability risk assessment methodology is applied to various examples consisting of university and industry projects in chapter 6. The application helps to reveal the strengths of the methodology and is termed ‘Verification of the methodology’. Validation issues of both the methodologies are dealt with in chapter 7 using the controlled experimental design. It is found that both the methodologies help to improve the quality of decision making during design evaluation. Relative reliability risk evaluation methodology helps to improve the quality of decision making to a substantial extent but physical realizability evaluation methodology shows only a little improvement in quality of decision making. Finally, it is suggested that the decomposed design evaluation methodology helps to improve the quality of decision making and is therefore proposed to be used by both novice and experienced designers.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Quantitative proteomic characterization of cellular pathways associated with altered insulin sensitivity in skeletal muscle following high-fat diet feeding and exercise training

Regular exercise elicits advantageous metabolic adaptations in skeletal muscle, such as improved insulin sensitivity. However, the underpinning molecular mechanisms and the effect of diet on muscle exercise training benefits are unclear. We therefore characterized the skeletal muscle proteome following exercise training (ET) in mice fed chow or high-fat diet (HFD). ET increased exercise performance, lowered body-weight, decreased fat mass and improved muscle insulin action in chow-and HFD-fed mice. At the molecular level, ET regulated 170 muscle proteins in chow-fed mice, but only 29 proteins in HFD-fed mice. HFD per se altered 56 proteins, most of which were regulated in a similar direction by ET. To identify proteins that might have particular health-related bearing on skeletal muscle metabolism, we filtered for differentially regulated proteins in response to ET and HFD. This yielded 15 proteins, including the major urinary protein 1 (MUP1), which was the protein most decreased after HFD, but increased with ET. The ET-induced Mup1 expression was absent in mouse muscle lacking functional AMPK. MUP1 also potentiated insulin-stimulated GLUT4 translocation in cultured muscle cells. Collectively, we provide a resource of ET-regulated proteins in insulin-sensitive and insulin-resistant skeletal muscle. The identification of MUP1 as a diet-, ET-and AMPK-regulated skeletal muscle protein that improves insulin sensitivity in muscle cells demonstrates the usefulness of these data