A Phase Diagram for Morphology and Properties of Low Temperature Deposited Polycrystalline Silicon Grown by Hot-wire Chemical Vapor Deposition

The fabrication of low temperature polycrystalline silicon with internal surface passivation and with lifetimes close to single crystalline silicon is a promising direction for thin film polycrystalline silicon photovoltaics. To achieve high lifetimes, large grains with passivated low-angle grain boundaries and intragranular defects are required. We investigate the low-temperature (300-475 °C) growth of thin silicon films by hot-wire chemical vapor deposition (HWCVD) on Si (100) substrates and on large-grained polycrystalline silicon template layers formed by selective nucleation and solid phase epitaxy (SNSPE). Phase diagrams for dilute silane deposition varying substrate temperature and for pure silane varying hydrogen dilution are shown. We will discuss the relationship between the microstructure and photoconductive decay lifetimes of these undoped layers on Si (100) and SNSPE templates as well as their suitability for use in thin-film photovoltaic applications

Radial pn junction nanorod solar cells: device physics principles and routes to fabrication in silicon

We have developed quantitative device-physics models for a radial pn junction nanorod solar cell, that is, a cell which consists of densely packed nanorods attached to a conducting substrate, each nanorod with a pn junction in the radial direction. It is found that this novel design shows large improvements over the planar geometry so long as two conditions are satisfied: a) a planar solar cell made from the same material is collection limited, i.e. the diffusion length of minority carriers is too low to allow for collection of most or all of the light-generated carriers in the conventional planar geometry, and b) recombination in the depletion region is not too high, or, equivalently, the lifetime of carriers in the depletion region is not too short. In order to experimentally validate this concept, the vapor-liquid-solid (VLS) growth of silicon (Si) nanorods has been explored using metal catalyst particles that are not as deleterious to the minority carrier lifetime of Si as gold (Au), the most commonly used wire growth catalyst

SOFAR float trajectories in the tropical Atlantic 1989-1992

Neutrally buoyant SOFAR floats at nominal depths of 800 m, 1800 m, and 3300 m were tracked acoustically for 3.7 years in the vicinity of the western boundary and the equator of the Atlantic Ocean. Trajectories and summaries from the whole experiment are shown along with detailed trajectories from the second setting of the listening stations, October 1990 to September 1992. Some highlights are mentioned below. Trajectories at 1800 m revealed a swift narrow southward flowing deep western boundary current (DWBC) extending from 7°N across the equator. Two floats directly crossed the equator in the DWBC and went to 10°S. Two other floats left the DWBC near the equator and drifted eastward. Three floats entered the DWBC from the equatorial current system and drifted southward. No obvious DWBC or swift equatorial currents were observed by the 3300 m floats. The 800 m floats plus some surface drifters measured seven anticyclonic eddies as they translated northwestward along the coast of South America in a band from the equator to 12°N. One of the floats (28) entered the Caribbean where tracking stopped. This float was again tracked as it drifted across the mid-Atlantic Ridge and entered the Canary Basin near 34°N 28°W after a gap of 2.7 years. We infer that this float went westward though the Caribbean and northeastward in the Gulf Stream. Float 17 drifted northward from 10°N to 22°N in an eastern boundary current off the coast of West Africa. Floats between 6°N-6°S (roughly) drifted long distances zonally in the equatorial current system.Funding was provided by the National Science Foundation through Grants Nos. OCE85-21082, OCE85-17375, and OCE91-14656

The Immune Adaptor SLP-76 Binds to SUMO-RANGAP1 at Nuclear Pore Complex Filaments to Regulate Nuclear Import of Transcription Factors in T Cells

While immune cell adaptors regulate proximal T cell signaling, direct regulation of the nuclear pore complex (NPC) has not been reported. NPC has cytoplasmic filaments composed of RanGAP1 and RanBP2 with the potential to interact with cytoplasmic mediators. Here, we show that the immune cell adaptor SLP-76 binds directly to SUMO-RanGAP1 of cytoplasmic fibrils of the NPC, and that this interaction is needed for optimal NFATc1 and NF-κB p65 nuclear entry in T cells. Transmission electron microscopy showed anti-SLP-76 cytoplasmic labeling of the majority of NPCs in anti-CD3 activated T cells. Further, SUMO-RanGAP1 bound to the N-terminal lysine 56 of SLP-76 where the interaction was needed for optimal RanGAP1-NPC localization and GAP exchange activity. While the SLP-76-RanGAP1 (K56E) mutant had no effect on proximal signaling, it impaired NF-ATc1 and p65/RelA nuclear entry and in vivo responses to OVA peptide. Overall, we have identified SLP-76 as a direct regulator of nuclear pore function in T cells

SOFAR float trajectories from an experiment to measure the Atlantic cross equatorial flow (1989-1990)

Neutrally buoyant SOFAR floats at nominal depths of 800, 1800, and 3300 m were tracked for 21 months in the vicinity of western boundary currents near 6N and at several sites in the Atlantic near 11N and along the equator. Trajectories at 1800 m show a swift (>50 cm/sec), narrow (100 km wide) southward-flowing Deep Western Boundary Current (DWBC) extending from 7N to the equator. At times (February-March 1989) DWBC water turned eastward and flowed along the equator and at other times (August-September 1990) the DWBC crossed the equator and continued southward. The mean velocity near the equator was eastward from February 1989 to February 1990 and westward from March 1990 to November 1990. Thus the cross-equatorial flow in the DWBC appeared to be linked to the direction of equatorial currents which varied over periods of more than a year. No obvious DWBC nor swift equatorial current was observed by 3300 m floats. Eight-hundred-meter floats revealed a northwestward intermediate level western boundary current although flow patterns were complicated. Three floats that significantly contributed to the northwestward flow looped in anticyclonic eddies that translated up the coast at 8 cm/sec. Six 800 m floats drifted eastward along the equator between 5S and 6N at a mean velocity of 11 cm/sec; one reached 5W in the Gulf of Guinea, suggesting that the equatorial current extended at least 35-40° along the equator. Three of these floats reversed direction near the end of the tracking period, implying low frequency fluctuations.Funding was provided by the National Science Foundation through Grant Nos. OCE-8521082, OCE-8517375, and OCE-9114656

Linoleic acid participates in the response to ischemic brain injury through oxidized metabolites that regulate neurotransmission.

Linoleic acid (LA; 18:2 n-6), the most abundant polyunsaturated fatty acid in the US diet, is a precursor to oxidized metabolites that have unknown roles in the brain. Here, we show that oxidized LA-derived metabolites accumulate in several rat brain regions during CO2-induced ischemia and that LA-derived 13-hydroxyoctadecadienoic acid, but not LA, increase somatic paired-pulse facilitation in rat hippocampus by 80%, suggesting bioactivity. This study provides new evidence that LA participates in the response to ischemia-induced brain injury through oxidized metabolites that regulate neurotransmission. Targeting this pathway may be therapeutically relevant for ischemia-related conditions such as stroke

Magnetic resonance imaging-guided phase 1 trial of putaminal AADC gene therapy for Parkinson's disease.

ObjectiveTo understand the safety, putaminal coverage, and enzyme expression of adeno-associated viral vector serotype-2 encoding the complementary DNA for the enzyme, aromatic L-amino acid decarboxylase (VY-AADC01), delivered using novel intraoperative monitoring to optimize delivery.MethodsFifteen subjects (three cohorts of 5) with moderately advanced Parkinson's disease and medically refractory motor fluctuations received VY-AADC01 bilaterally coadministered with gadoteridol to the putamen using intraoperative magnetic resonance imaging (MRI) guidance to visualize the anatomic spread of the infusate and calculate coverage. Cohort 1 received 8.3 × 1011 vg/ml and ≤450 μl per putamen (total dose, ≤7.5 × 1011 vg); cohort 2 received the same concentration (8.3 × 1011 vg/ml) and ≤900 μl per putamen (total dose, ≤1.5 × 1012 vg); and cohort 3 received 2.6 × 1012 vg/ml and ≤900 μl per putamen (total dose, ≤4.7 × 1012 vg). (18)F-fluoro-L-dihydroxyphenylalanine positron emission tomography (PET) at baseline and 6 months postprocedure assessed enzyme activity; standard assessments measured clinical outcomes.ResultsMRI-guided administration of ascending VY-AADC01 doses resulted in putaminal coverage of 21% (cohort 1), 34% (cohort 2), and 42% (cohort 3). Cohorts 1, 2, and 3 showed corresponding increases in enzyme activity assessed by PET of 13%, 56%, and 79%, and reductions in antiparkinsonian medication of -15%, -33%, and -42%, respectively, at 6 months. At 12 months, there were dose-related improvements in clinical outcomes, including increases in patient-reported ON-time without troublesome dyskinesia (1.6, 3.3, and 1.5 hours, respectively) and quality of life.InterpretationNovel intraoperative monitoring of administration facilitated targeted delivery of VY-AADC01 in this phase 1 study, which was well tolerated. Increases in enzyme expression and clinical improvements were dose dependent. ClinicalTrials.gov Identifier: NCT01973543 Ann Neurol 2019;85:704-714

Cadherin-Mediated Differential Cell Adhesion Controls Slow Muscle Cell Migration in the Developing Zebrafish Myotome

AbstractSlow-twitch muscle fibers of the zebrafish myotome undergo a unique set of morphogenetic cell movements. During embryogenesis, slow-twitch muscle derives from the adaxial cells, a layer of paraxial mesoderm that differentiates medially within the myotome, immediately adjacent to the notochord. Subsequently, slow-twitch muscle cells migrate through the entire myotome, coming to lie at its most lateral surface. Here we examine the cellular and molecular basis for slow-twitch muscle cell migration. We show that slow-twitch muscle cell morphogenesis is marked by behaviors typical of cells influenced by differential cell adhesion. Dynamic and reciprocal waves of N-cadherin and M-cadherin expression within the myotome, which correlate precisely with cell migration, generate differential adhesive environments that drive slow-twitch muscle cell migration through the myotome. Removing or altering the expression of either protein within the myotome perturbs migration. These results provide a definitive example of homophilic cell adhesion shaping cellular behavior during vertebrate development

Pharmacists in Pharmacovigilance: Can Increased Diagnostic Opportunity in Community Settings Translate to Better Vigilance?

The pharmacy profession has undergone substantial change over the last two to three decades. Whilst medicine supply still remains a central function, pharmacist’s roles and responsibilities have become more clinic and patient focused. In the community (primary care), pharmacists have become important providers of healthcare as Western healthcare policy advocates patient self-care. This has resulted in pharmacists taking on greater responsibility in managing minor illness and the delivery of public health interventions. These roles require pharmacists to more fully use their clinical skills, and often involve diagnosis and therapeutic management. Community pharmacists are now, more than ever before, in a position to identify, record and report medication safety incidents. However, current research suggests that diagnostic ability of community pharmacists is questionable and they infrequently report to local or national schemes. The aim of this paper is to highlight current practice and suggest ways in which community pharmacy can more fully contribute to patient safety