Roadmap to a One Health agenda 2030

The current fragmented framework of health governance for humans, animals and environment, together with the conventional linear approach to solving current health problems, is failing to meet today's health challenges and is proving unsustainable. Advances in healthcare depend increasingly on intensive interventions, technological developments and expensive pharmaceuticals. The disconnect grows between human health, animal health and environmental and ecosystems health. Human development gains have come with often unrecognized negative externalities affecting ecosystems. Deterioration in biodiversity and ecosystem services threatens to reverse the health gains of the last century. A paradigm shift is urgently required to de-sectoralize human, animal, plant and ecosystem health and to take a more integrated approach to health, One Health (OH). The Sustainable Development Goals (SDGs) offer a framework and unique opportunity for this. Through analysing individual SDGs, we argue the feasibility of an OH approach towards achieving them. Feasibility assessments and outcome evaluations are often constrained by sectoral politics within a national framework, historic possession of expertise, as well as tried and tested metrics. OH calls for a better understanding, acceptance and use of a broader and transdisciplinary set of assessment metrics. Key objectives of OH are presented: that humans reconnect with our natural past and accept our place in, and dependence on our planet's ecosystems; and that we recognize our dependence on ecosystem services, the impact of our development thereon and accept our responsibility towards future generations to address this. Several action points are proposed to meet these objectives.peer-reviewe

Interleukin 2-regulated in vitro antibody production following a single spinal manipulative treatment in normal subjects

<p>Abstract</p> <p>Background</p> <p>Our recent investigations have demonstrated that cell cultures from subjects, who received a single spinal manipulative treatment in the upper thoracic spine, show increased capacity for the production of the key immunoregulatory cytokine, interleukin-2. However, it has not been determined if such changes influence the response of the immune effector cells. Thus, the purpose of the present study was to determine whether, in the same subjects, spinal manipulation-related augmentation of the <it>in vitro </it>interleukin-2 synthesis is associated with the modulation of interleukin 2-dependent and/or interleukin-2-induced humoral immune response (antibody synthesis).</p> <p>Methods</p> <p>A total of seventy-four age and sex-matched healthy asymptomatic subjects were studied. The subjects were assigned randomly to: venipuncture control (n = 22), spinal manipulative treatment without cavitation (n = 25) or spinal manipulative treatment associated with cavitation (n = 27) groups. Heparinized blood samples were obtained from the subjects before (baseline) and then at 20 minutes and 2 hours post-treatment. Immunoglobulin (antibody) synthesis was induced in cultures of peripheral blood mononuclear cells by stimulation with conventional pokeweed mitogen or by application of human recombinant interleukin-2. Determinations of the levels of immunoglobulin G and immunoglobulin M production in culture supernatants were performed by specific immunoassays.</p> <p>Results</p> <p>The baseline levels of immunoglobulin synthesis induced by pokeweed mitogen or human recombinant interleukin-2 stimulation were comparable in all groups. No significant changes in the production of pokeweed mitogen-induced immunoglobulins were observed during the post-treatment period in any of the study groups. In contrast, the production of interleukin-2 -induced immunoglobulin G and immunoglobulin M was significantly increased in cultures from subjects treated with spinal manipulation. At 20 min post-manipulation, immunoglobulin G synthesis was significantly elevated in subjects who received manipulation with cavitation, relative to that in cultures from subjects who received manipulation without cavitation and venipuncture alone. At 2 hr post-treatment, immunoglobulin M synthesis was significantly elevated in subjects who received manipulation with cavitation relative to the venipuncture group. There were no quantitative alterations within the population of peripheral blood B or T lymphocytes in the studied cultures.</p> <p>Conclusion</p> <p>Spinal manipulative treatment does not increase interleukin-2 -dependent polyclonal immunoglobulin synthesis by mitogen-activated B cells. However, antibody synthesis induced by interleukin-2 alone can be, at least temporarily, augmented following spinal manipulation. Thus, under certain physiological conditions spinal manipulative treatment might influence interleukin-2 -regulated biological responses.</p

Prevalence of contagious and environmental mastitis-causing bacteria in bulk tank milk and its relationships with milking practices of dairy cattle herds in São Miguel Island (Azores)

This study aimed to assess the degree of contamination of bulk tank milk (BTM) by Staphylococcus spp. and coliform bacteria and to identify major milking practices that help perpetuate them in dairy cattle herds in São Miguel Island. In July 2014, BTM was sampled and a survey concerning local milking practices was conducted on 100 herds. Semi quantitative multiplex polymerase chain reaction detected coagulase-negative staphylococci, Escherichia coli, Staphylococcus aureus, and other coliform bacteria (Klebsiella oxytoca, Klebsiella pneumoniae, andSerratia marcescens) in 100, 75, 59, and 35 % of BTM, respectively. According to multivariable univariate models, on herds not using hot water for cleaning the milking machine and teat liners, there was at least 3.4 more odds (P<0.01) to have S. aureus or coliform bacteria contamination in BTM. The likelihoodoffinding S.aureus inBTMwas higher(P<0.001)on herds without high hygiene during milking, when milking mastitic cows at the end, on abrupt cessation of milking at dry-off, and official milk control implementation. The glove use also favored (odds ratio (OR) 5.8; P<0.01)thedetection ofcoliformbacteriainBTM.Poormilkingpracticesidentified in this study should be avoided in order to decrease S. aureus and coliform bacteria contamination of BTM. Other factors associated with milk quality in São Miguel Island also should be further investigated

Autoantibodies to Agrin in Myasthenia Gravis Patients

To determine if patients with myasthenia gravis (MG) have antibodies to agrin, a proteoglycan released by motor neurons and is critical for neuromuscular junction (NMJ) formation, we collected serum samples from 93 patients with MG with known status of antibodies to acetylcholine receptor (AChR), muscle specific kinase (MuSK) and lipoprotein-related 4 (LRP4) and samples from control subjects (healthy individuals and individuals with other diseases). Sera were assayed for antibodies to agrin. We found antibodies to agrin in 7 serum samples of MG patients. None of the 25 healthy controls and none of the 55 control neurological patients had agrin antibodies. Two of the four triple negative MG patients (i.e., no detectable AChR, MuSK or LRP4 antibodies, AChR-/MuSK-/LRP4-) had antibodies against agrin. In addition, agrin antibodies were detected in 5 out of 83 AChR+/MuSK-/LRP4- patients but were not found in the 6 patients with MuSK antibodies (AChR-/MuSK+/LRP4-). Sera from MG patients with agrin antibodies were able to recognize recombinant agrin in conditioned media and in transfected HEK293 cells. These sera also inhibited the agrin-induced MuSK phosphorylation and AChR clustering in muscle cells. Together, these observations indicate that agrin is another autoantigen in patients with MG and agrin autoantibodies may be pathogenic through inhibition of agrin/LRP4/MuSK signaling at the NMJ

Reported anxiety on work shifts for coronary care nurses

The purpose of the study was to investigate the perceived anxiety experienced by coronary care nurses working eight- and 12-hour work shifts. It was hypothesized that length of shift work affects the before- and after-shift anxiety for nurses working eight- or 12-hour work shifts. Anxiety differences between the eight- and 12-hour work shifts were examined as well as differences between the before- and after-shift anxiety for each length of shift.Thirty-two (32) coronary care nurses from a general medical and surgical hospital in the Midwest volunteered to participate in the research. The sample consisted of all females (18 Registered Nurses, nine Licensed Practical Nurses, three charge nurses, a supervisory nurse, and a rehabilitation nurse). The nurses had voluntarily selected the eight- or 12-hour length of shift work and understood that they would be committed towork that shift for one year. Twelve nurses worked the eight-hour shift; 20 nurses chose the new 12-hour shift.The State Anxiety Inventory (SAI) was used to measure the nurses' before- and after-shift anxiety. Baseline data were collected from the nurses prior to the initiation of the 12-hour shift. The Box-Jenkins Time Series Analysis was used to analyze the daily before- and after-shift anxiety scores and to establish a forecasted trend for both the eight- and 12-hour shifts.The first finding was that the before-shift anxiety scores for the eight-hour workers declined, while the anxiety scores for the 12-hour workers increased, following the implementation of the 12-hour shift. The same trend was forecast for the after-shift anxiety scores. The eight-hour nurses' after-shift anxiety scores declined while the 12-hour nurses' anxiety scores increased. A transfer function was conducted for both the eight-hour and 12-hour before- and after-shift anxiety scores. No predictive trend could be established for the eight-hour anxiety scores; however, the 12-hour after-shift anxiety scores were found to be consistently higher than before-shift anxiety scores.Thesis (Ph. D.)Department of Counseling Psychology and Guidance Service

L’émiettement urbain ? Ville compacte et ville diffuse sont dans le même bateau

La ville compacte et la ville diffuse sont les deux faces indissociables de l’urbanisation contemporaine. Pour défendre ce point de vue, notre article revient d’abord sur la notion d’échelle géographique. Genève présente l’exemple d’une ville-centre très dense et d’une agglomération transfrontalière étalée, dont l’existence fut longtemps masquée par la présence d’une frontière nationale. En second lieu, nous présentons l’engouement pour la ville compacte et le rejet de la ville diffuse comme des instruments d’une « économie de la rareté ». Celle-ci entretient la gentrification dans les centres et la dispersion dans des périurbains toujours plus lointains. En troisième lieu, nous discutons l’hypothèse proposée par ce numéro thématique selon laquelle l’agriculture urbaine contribuerait à l’émergence d’une ville diffuse durable. Un enthousiasme aveugle pour cette piste pourrait, si elle n’est pas bien évaluée, perpétuer le processus global d’émiettement urbain et entraver l’identification d’approches innovantes pour faire face aux défis du moment.To consider the compact city as an alternative to suburbia is to underestimate contemporary processes of urbanization. The compact city and suburbia are two sides of the same coin. To illustrate this argument, we first consider the example of geographic scale. Depending on the scale one adopts for the analysis, Geneva can be considered to be either an exemplary compact city or truly representative of suburban sprawl. Our second point identifies the “economics of scarcity” as a factor contributing both to gentrification in inner cities and to the never-ending expansion of suburbia. Third, while the idea of making suburbia more sustainable and implementing urban agriculture may sound attractive, we suggest that these approaches should be used very carefully. Otherwise, they may well reproduce the very centrifugal mechanisms they are trying to counteract and so hinder the search for more innovative approaches.La ciudad compacta y la cuidad difusa son las dos caras indisociables de la urbanización contemporánea. Para defender este punto de vista, nuestro artículo reasume, primeramente, la noción de escala geográfica. Génova ejemplariza una ciudad-centro muy densa y con una aglomeración transfrontalera extendida, cuya existencia fue ocultada, desde hace tiempo, por la presencia de una frontera nacional. En segundo lugar, presentamos el entusiasmo por la ciudad compacta y el rechazo de la ciudad difusa como instrumentos de una “economía de la rareza”. Esta última mantiene la gentrificación* en los centros y la dispersión en lo periurbano, cada vez más alejado. En tercer lugar, discutimos la hipótesis propuesta por ese número de CGQ según la cual la “agricultura urbana” contribuiría al nacimiento de una “ciudad difusa sostenible”. Un entusiasmo ciego por esta pista podría, si no está bien evaluada, perpetuar el proceso global de desagregación urbana y echar traba al descubrimiento de enfoques novadores para enfrente los desafíos del momento.*aburguesamiento (nota de traducción)