5,979 research outputs found
Promoting Microbiology Education Through the iGEM Synthetic Biology Competition
Synthetic biology has developed rapidly in the 21st century. It covers a range of scientific disciplines that incorporate principles from engineering to take advantage of and improve biological systems, often applied to specific problems. Methods important in this subject area include the systematic design and testing of biological systems and, here, we describe how synthetic biology projects frequently develop microbiology skills and education. Synthetic biology research has huge potential in biotechnology and medicine, which brings important ethical and moral issues to address, offering learning opportunities about the wider impact of microbiological research. Synthetic biology projects have developed into wide-ranging training and educational experiences through iGEM, the International Genetically Engineered Machines competition. Elements of the competition are judged against specific criteria and teams can win medals and prizes across several categories. Collaboration is an important element of iGEM and all DNA constructs synthesised by iGEM teams are made available to all researchers through the Registry for Standard Biological Parts. An overview of microbiological developments in the iGEM competition is provided. This review is targeted at educators that focus on microbiology and synthetic biology, but will also be of value to undergraduate and postgraduate students with an interest in this exciting subject area
Microbiology survey shows authors have most to gain from peer review
In the week that an international team of publishers, science communicators and other scholarly organisations launch the second international Peer Review Week, the preliminary findings from our new survey reveal that authors gain more from peer review than the people who do the actual review
Characterization of a temperature-sensitive DNA ligase from Escherichia coli
DNA ligases are essential enzymes in cells due to their ability to join DNA strand breaks formed during DNA replication. Several temperature-sensitive mutant strains of Escherichia coli, including strain GR501, have been described which can be complemented by functional DNA ligases. Here, it is shown that the ligA251 mutation in E. coli GR501 strain is a cytosine to thymine transition at base 43, which results in a substitution of leucine by phenylalanine at residue 15. The protein product of this gene (LigA251) is accumulated to a similar level at permissive and non-permissive temperatures. Compared to wild-type LigA, at 20 Ā°C purified LigA251 has 20-fold lower ligation activity in vitro, and its activity is reduced further at 42 Ā°C, resulting in 60-fold lower ligation activity than wild-type LigA. It is proposed that the mutation in LigA251 affects the structure of the N-terminal region of LigA. The resulting decrease in DNA ligase activity at the non-permissive temperature is likely to occur as the result of a conformational change that reduces the rate of adenylation of the ligase
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Advances in understanding large-scale responses of the water cycle to climate change
Globally, thermodynamics explains an increase in atmospheric water vapor with warming of around 7%/Ā°C near to the surface. In contrast, global precipitation and evaporation are constrained by the Earth's energy balance to increase at ā¼2ā3%/Ā°C. However, this rate of increase is suppressed by rapid atmospheric adjustments in response to greenhouse gases and absorbing aerosols that directly alter the atmospheric energy budget. Rapid adjustments to forcings, cooling effects from scattering aerosol, and observational uncertainty can explain why observed global precipitation responses are currently difficult to detect but are expected to emerge and accelerate as warming increases and aerosol forcing diminishes. Precipitation increases with warming are expected to be smaller over land than ocean due to limitations on moisture convergence, exacerbated by feedbacks and affected by rapid adjustments. Thermodynamic increases in atmospheric moisture fluxes amplify wet and dry events, driving an intensification of precipitation extremes. The rate of intensification can deviate from a simple thermodynamic response due to ināstorm and largerāscale feedback processes, while changes in largeāscale dynamics and catchment characteristics further modulate the frequency of flooding in response to precipitation increases. Changes in atmospheric circulation in response to radiative forcing and evolving surface temperature patterns are capable of dominating water cycle changes in some regions. Moreover, the direct impact of human activities on the water cycle through water abstraction, irrigation, and land use change is already a significant component of regional water cycle change and is expected to further increase in importance as water demand grows with global population
Fourth-Generation Fan Assessment Numeration System (FANS) Design and Performance Specifications
The Fan Assessment Numeration System (FANS) is a measurement device for generating ventilation fan performance curves. Three different-sized FANS currently exist for assessing ventilation fans commonly used in poultry and livestock housing systems. All FANS consist of an array of anemometers inside an aluminum shroud that traverse the inlet or outlet of a ventilation fan. The FANS design has been updated several times since its inception and is currently in its fourth-generation (G4). The current design iteration (FANS-G4) is reported in this article with an emphasis on the hardware and software control, data acquisition systems, and operational reliability. Six FANS-G4 units were fabricated at the University of Kentucky (UK) Agricultural Machinery Research Laboratory and calibrated at the University of Illinois Urbana-Champaign (UIUC) Bioenvironmental and Structural Systems (BESS) Laboratory. Results demonstrated that the FANS-G4 was capable of measuring volumetric airflow to within 0.6% of full-scale (FS), which ranged from 15,000 to 56,000 m3 h-1
The Next Generation Virgo Cluster Survey. XII. Stellar Populations and Kinematics of Compact, Low-Mass Early-Type Galaxies from Gemini GMOS-IFU Spectroscopy
We present Gemini GMOS-IFU data of eight compact low-mass early-type galaxies
(ETGs) in the Virgo cluster. We analyse their stellar kinematics, stellar
population, and present two-dimensional maps of these properties covering the
central 5"x 7" region. We find a large variety of kinematics: from non- to
highly-rotating objects, often associated with underlying disky isophotes
revealed by deep images from the Next Generation Virgo Cluster Survey. In half
of our objects, we find a centrally-concentrated younger and more metal-rich
stellar population. We analyze the specific stellar angular momentum through
the lambdaR parameter and find six fast-rotators and two slow-rotators, one
having a thin counter-rotating disk. We compare the local galaxy density and
stellar populations of our objects with those of 39 more extended low-mass
Virgo ETGs from the SMAKCED survey and 260 massive (\Msun) ETGs from
the A3D sample. The compact low-mass ETGs in our sample are located in high
density regions, often close to a massive galaxy and have, on average, older
and more metal-rich stellar populations than less compact low-mass galaxies. We
find that the stellar population parameters follow lines of constant velocity
dispersion in the mass-size plane, smoothly extending the comparable trends
found for massive ETGs. Our study supports a scenario where low-mass compact
ETGs have experienced long-lived interactions with their environment, including
ram-pressure stripping and gravitational tidal forces, that may be responsible
for their compact nature.Comment: Accepted in ApJ, 19 pages, 10 figure
The Design and Validation of the Quantum Mechanics Conceptual Survey
The Quantum Mechanics Conceptual Survey (QMCS) is a 12-question survey of
students' conceptual understanding of quantum mechanics. It is intended to be
used to measure the relative effectiveness of different instructional methods
in modern physics courses. In this paper we describe the design and validation
of the survey, a process that included observations of students, a review of
previous literature and textbooks and syllabi, faculty and student interviews,
and statistical analysis. We also discuss issues in the development of specific
questions, which may be useful both for instructors who wish to use the QMCS in
their classes and for researchers who wish to conduct further research of
student understanding of quantum mechanics. The QMCS has been most thoroughly
tested in, and is most appropriate for assessment of (as a posttest only),
sophomore-level modern physics courses. We also describe testing with students
in junior quantum courses and graduate quantum courses, from which we conclude
that the QMCS may be appropriate for assessing junior quantum courses, but is
not appropriate for assessing graduate courses. One surprising result of our
faculty interviews is a lack of faculty consensus on what topics should be
taught in modern physics, which has made designing a test that is valued by a
majority of physics faculty more difficult than expected.Comment: Submitted to Physical Review Special Topics: Physics Education
Researc
An X-Ray View of the Jet-Cycle in the Radio Loud AGN 3C120
We present a study of the central engine in the broad-line radio galaxy 3C120
using a multi-epoch analysis of a deep XMM-Newton observation and two deep
Suzaku pointings (in 2012). In order to place our spectral data into the
context of the disk-disruption/jet-ejection cycles displayed by this object, we
monitor the source in the UV/X-ray bands, and in the radio band. We find three
statistically acceptable spectral models, a disk-reflection model, a jet-model
and a jet+disk model. Despite being good descriptions of the data, the
disk-reflection model violates the radio constraints on the inclination, and
the jet-model has a fine-tuning problem, requiring a jet contribution exceeding
that expected. Thus, we argue for a composite jet+disk model. Within the
context of this model, we verify the basic predictions of the jet-cycle
paradigm, finding a truncated/refilling disk during the Suzaku observations and
a complete disk extending down to the innermost stable circular orbit (ISCO)
during the XMM-Newton observation. The idea of a refilling disk is further
supported by the detection of the ejection of a new jet knot approximately one
month after the Suzaku pointings. We also discover a step-like event in one of
the Suzaku pointings in which the soft band lags the hard band. We suggest that
we are witnessing the propagation of a disturbance from the disk into the jet
on a timescale set by the magnetic field.Comment: 14 pages, 10 figures, accepted for publication in Ap
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