17 research outputs found
Characterization of THB1, a Chlamydomonas reinhardtii truncated hemoglobin: linkage to nitrogen metabolism and identification of lysine as the distal heme ligand
The nuclear genome of the model organism Chlamydomonas reinhardtii contains genes for a dozen hemoglobins of the truncated lineage. Of those, THB1 is known to be expressed, but the product and its function have not yet been characterized. We present mutagenesis, optical, and nuclear magnetic resonance data for the recombinant protein and show that at pH near neutral in the absence of added ligand, THB1 coordinates the heme iron with the canonical proximal histidine and a distal lysine. In the cyanomet state, THB1 is structurally similar to other known truncated hemoglobins, particularly the heme domain of Chlamydomonas eugametos LI637, a light-induced chloroplastic hemoglobin. Recombinant THB1 is capable of binding nitric oxide (NO(*)) in either the ferric or ferrous state and has efficient NO(*) dioxygenase activity. By using different C. reinhardtii strains and growth conditions, we demonstrate that the expression of THB1 is under the control of the NIT2 regulatory gene and that the hemoglobin is linked to the nitrogen assimilation pathway
First Plant Cell Atlas symposium report
The Plant Cell Atlas (PCA) community hosted a virtual symposium on December 9 and 10, 2021 on single cell and spatial omics technologies. The conference gathered almost 500 academic, industry, and government leaders to identify the needs and directions of the PCA community and to explore how establishing a data synthesis center would address these needs and accelerate progress. This report details the presentations and discussions focused on the possibility of a data synthesis center for a PCA and the expected impacts of such a center on advancing science and technology globally. Community discussions focused on topics such as data analysis tools and annotation standards; computational expertise and cyber-infrastructure; modes of community organization and engagement; methods for ensuring a broad reach in the PCA community; recruitment, training, and nurturing of new talent; and the overall impact of the PCA initiative. These targeted discussions facilitated dialogue among the participants to gauge whether PCA might be a vehicle for formulating a data synthesis center. The conversations also explored how online tools can be leveraged to help broaden the reach of the PCA (i.e., online contests, virtual networking, and social media stakeholder engagement) and decrease costs of conducting research (e.g., virtual REU opportunities). Major recommendations for the future of the PCA included establishing standards, creating dashboards for easy and intuitive access to data, and engaging with a broad community of stakeholders. The discussions also identified the following as being essential to the PCA’s success: identifying homologous cell-type markers and their biocuration, publishing datasets and computational pipelines, utilizing online tools for communication (such as Slack), and user-friendly data visualization and data sharing. In conclusion, the development of a data synthesis center will help the PCA community achieve these goals by providing a centralized repository for existing and new data, a platform for sharing tools, and new analytical approaches through collaborative, multidisciplinary efforts. A data synthesis center will help the PCA reach milestones, such as community-supported data evaluation metrics, accelerating plant research necessary for human and environmental health
Vision, challenges and opportunities for a Plant Cell Atlas
With growing populations and pressing environmental problems, future economies will be increasingly plant-based. Now is the time to reimagine plant science as a critical component of fundamental science, agriculture, environmental stewardship, energy, technology and healthcare. This effort requires a conceptual and technological framework to identify and map all cell types, and to comprehensively annotate the localization and organization of molecules at cellular and tissue levels. This framework, called the Plant Cell Atlas (PCA), will be critical for understanding and engineering plant development, physiology and environmental responses. A workshop was convened to discuss the purpose and utility of such an initiative, resulting in a roadmap that acknowledges the current knowledge gaps and technical challenges, and underscores how the PCA initiative can help to overcome them.</jats:p
Intact Protein Analysis by Mass Spectrometry to Characterize the Truncated Hemoglobin THB1 from Chlamydomonas Reinhardtii
Helix-Capping Histidines: Diversity of N–H···N Hydrogen Bond Strength Revealed by <sup>2h</sup><i>J</i><sub>NN</sub> Scalar Couplings
In addition to its well-known roles
as an electrophile and general
acid, the side chain of histidine often serves as a hydrogen bond
(H-bond) acceptor. These H-bonds provide a convenient pH-dependent
switch for local structure and functional motifs. In hundreds of instances,
a histidine caps the N-terminus of α- and 3<sub>10</sub>-helices
by forming a backbone NH···Nδ1 H-bond. To characterize
the resilience and dynamics of the histidine cap, we measured the <i>trans</i> H-bond scalar coupling constant, <sup>2h</sup><i>J</i><sub>NN</sub>, in several forms of Group 1 truncated hemoglobins
and cytochrome <i>b</i><sub>5</sub>. The set of 19 measured <sup>2h</sup><i>J</i><sub>NN</sub> values were between 4.0 and
5.4 Hz, generally smaller than in nucleic acids (∼6–10
Hz) and indicative of longer, weaker bonds in the studied proteins.
A positive linear correlation between <sup>2h</sup><i>J</i><sub>NN</sub> and the difference in imidazole ring <sup>15</sup>N
chemical shift (Δ<sup>15</sup>N = |δ<sup>15</sup>Nδ1
– δ<sup>15</sup>Nε2|) was found to be consistent
with variable H-bond length and variable cap population related to
the ionization of histidine in the capping and noncapping states.
The relative ease of <sup>2h</sup><i>J</i><sub>NN</sub> detection
suggests that this parameter can become part of the standard arsenal
for describing histidines in helix caps and other key structural and
catalytic elements involving NH···N H-bonds. The combined
nucleic acid and protein data extend the utility of <sup>2h</sup><i>J</i><sub>NN</sub> as a sensitive marker of local structural,
dynamic, and thermodynamic properties in biomolecules
Helix-Capping Histidines: Diversity of NHN Hydrogen Bond Strength Revealed by 2hJNN Scalar Couplings
Recommended from our members
Omega-3 Fatty Acids Activate Ciliary FFAR4 to Control Adipogenesis
Adult mesenchymal stem cells, including preadipocytes, possess a cellular sensory organelle called the primary cilium. Ciliated preadipocytes abundantly populate perivascular compartments in fat and are activated by a high-fat diet. Here, we sought to understand whether preadipocytes use their cilia to sense and respond to external cues to remodel white adipose tissue. Abolishing preadipocyte cilia in mice severely impairs white adipose tissue expansion. We discover that TULP3-dependent ciliary localization of the omega-3 fatty acid receptor FFAR4/GPR120 promotes adipogenesis. FFAR4 agonists and ω-3 fatty acids, but not saturated fatty acids, trigger mitosis and adipogenesis by rapidly activating cAMP production inside cilia. Ciliary cAMP activates EPAC signaling, CTCF-dependent chromatin remodeling, and transcriptional activation of PPARγ and CEBPα to initiate adipogenesis. We propose that dietary ω-3 fatty acids selectively drive expansion of adipocyte numbers to produce new fat cells and store saturated fatty acids, enabling homeostasis of healthy fat tissue