4,124 research outputs found
Copping with extreme dehydration:a physiological, biochemical and molecular study on the aquatic bryophyte Fontinalis antipyretica
Tese de doutoramento, Biologia (Fisiologia e Bioquímica), Universidade de Lisboa, Faculdade de Ciências, 2013Water is the most important element for life. During land invasion, the first plants had
to face an extreme dry environment, undergoing desiccation, a process in which tissues
virtually lose almost all water. Therefore, plants developed desiccation tolerance (DT)
mechanisms through which they could experience the dry state and return to normal function
upon rehydration. Nowadays, the organisms that have the ability to tolerate desiccation
include members of different taxa such as microbes, lichens, bryophytes, vascular plants and
animals. Nevertheless, DT is more frequent in lichens and bryophytes. Researchers have
been closely studying DT mechanisms hoping to identify new genes that can be transferred
through biotechnology to crop species, increasing their drought tolerance.
In the Mediterranean region, the aquatic species Fontinalis antipyretica is periodically
exposed to desiccation, in intermittent streams that lose their water during the dry season.
Field observations suggest DT mechanisms in this aquatic bryophyte. The main objective of
this Thesis is to study DT through the combination of physiological, biochemical and
molecular techniques in a bryophyte from a habitat not usually reported as prone to
desiccation, comparing with bryophytes already studied and described as desiccation
tolerant. Moreover, this integrated study aims to identify DT processes/mechanisms that are
common across desiccation tolerant plant species.
Photosynthesis is a very sensitive indicator of desiccation. The measurement of the
oxygen production rate, coupled with chlorophyll a fluorescence, allowed to follow the
photosynthetic response through non-invasive techniques (Chapter 2). Examining the
metabolic response to desiccation, we aimed to establish if F. antipyretica was DT and
whether the recovery of this species was mainly determined by the extent or by the rate of
dehydration, or by both. Our findings showed that the metabolic response of F. antipyretica
to desiccation, both under field and laboratory conditions, is consistent with a DT pattern. It
was concluded that dehydration must proceed slowly for the bryophyte to regain its predesiccation
state following rehydration. This was further confirmed in field-desiccated
samples which showed a similar recovery pattern as slow dehydration. This physiological
study of a widely distributed aquatic bryophyte periodically subjected to desiccation
contributed to improve our knowledge about dehydration rate in bryophyte survival.
The metabolic impairment observed during dehydration led to an increase in reactive
oxygen species (ROS) production (Chapter 3). It was found that managing oxidative stress
is one critical aspect for surviving desiccation. Although ROS production in response to
desiccation/rehydration has been determined in terrestrial bryophytes it was not investigated
in aquatic bryophytes. In addition, there were no published studies examining the impact of
dehydration rate on ROS production in bryophytes previously subjected to desiccation and
no information on the impact of drying rate on its production. Since it was determined that dehydration rate is fundamental for surviving DT, we investigated whether this response
included an oxidative burst sensitive to dehydration rate using an innovative approach
combining ROS-specific probes and confocal microscopy. The response was a very high
ROS production under fast dehydration whereas under slow dehydration was almost absent.
ROS react with cellular constituents, such as protein and lipids, leading to damage
and, thus, affecting directly cell survival. After observing the effect of dehydration rate on
ROS production, the next step was to test if the drying rate affected membrane damage
(Chapter 4). We measured lipid peroxidation as well as cation dynamics and conductivity
measurements to assess membrane damage and permeability. Since NO as also been
associated with DT and a possible role in membrane protection, we measure and discuss
the possible roles of its production and action. We also found that the increase in ROS and
red autofluorescence can be used in future works in desiccation and drought stress as an
indicator for detecting membrane damage and cell rupture.
During dehydration, cell water relations change occurring in some cases osmotic
adjustment through sucrose accumulation. Sucrose has an important role in DT by
preventing denaturation of macromolecules and slowing down damaging reactions with ROS.
We investigated how contrasting (fast and slow) dehydration rates change cell water relations
and sucrose content in an aquatic bryophyte and if those changes can explain the
requirement of slow dehydration to induce DT (Chapter 5). The characteristics of the
bryophytes cell walls appear to change under fast dehydration, allowing it to become more
elastic which probably allows to maintain a functional metabolism to lower water potentials.
Sucrose also increases under fast dehydration which can be an attempt to minimize damage
when time for a more effective protection is not available.
The theory for DT was based in a constitutive protection mechanism that would allow
the bryophyte to tolerate and protect the structures during desiccation, coupled with a repairbased
mechanism upon rehydration which would repair damage that accumulated during the
dried state. This would be controlled at the transcriptional level by accumulation of mRNA
that would be activated during rehydration. However, recently the study of proteomes in
bryophytes in response to dehydration suggests another approach to DT. We investigated
the effect of fast and slow drying rates on the protein profiles, considering both dehydration
and rehydration (Chapter 6). After fast dehydration, the proteome profiles of F. antipyretica
are very similar to control. However, rehydration following fast dehydration leads to loss of
almost all proteins, providing evidence that the bryophyte does not have enough time to
prepare for desiccation under this dehydration regime. Nevertheless, under slow dehydration
there are substantial changes in the proteome profile, both during dehydration and
rehydration which might indicate an induction of DT mechanisms under these circumstances.
The development of desiccation tolerance mechanisms that involved the accumulation of
specific proteins during cycles of dehydration/rehydration allowed land colonization by early bryophyte ancestors. The basis for such tolerance relies on common patterns of protein
expression and metabolic adjustments which are very similar even in bryophytes from very
distinct habitats. During dehydration, photosynthesis shuts down, high levels of soluble
sugars occur in the cytoplasm, defence proteins increase, cytoskeleton is disassembled and
sugar metabolism enzymes are up-regulated. After rehydration, photosynthesis restart,
cytoskeleton is re-assembled, high levels of: soluble sugars, sugar metabolism enzymes and
defence proteins are maintained.
The main conclusion suggested by this work is that DT at the cellular level, namely
at the level of the molecular mechanisms, is similar among bryophytes independently of their
preferred habitat. Furthermore, it states that DT is induced by slow dehydration rate being
eventually controlled to some point by the morphology, being the determinant factor in the
adaptation of bryophytes to each habitat and desiccation conditions.A água é o elemento mais importante para a Vida na Terra. Durante a ocupação do
meio terrestre, as primeiras plantas enfrentaram um ambiente extremamente árido levandoas
à dessecação, um estado no qual os tecidos perdem praticamente toda a água. Desta
forma, foram-se desenvolvendo mecanismos de tolerância à dessecação (DT, do inglês
desiccation tolerance) nas plantas, graças aos quais estas podem submeter-se ao estado
de dessecação e regressar a um funcionamento normal após a reidratação. Atualmente, os
organismos que apresentam DT incluem membros de taxa muito distintos, tais como
micróbios, briófitos, líquenes, plantas vasculares e animais. No entanto, a DT é mais
frequente nos líquenes e nos briófitos. Os investigadores têm estudado os mecanismos de
DT na esperança de descobrir novos genes que possam ser transferidos por meio da
biotecnologia para espécies vegetais de interesse agrícola, aumentando sua tolerância à
seca.
Na região do Mediterrâneo, a espécie aquática de briófito Fontinalis antipyretica é
periodicamente exposta à dessecação, em riachos que perdem a água durante a estação
seca. Observações no campo sugerem mecanismos de DT neste briófito aquático. O
objetivo principal desta Tese é estudar a DT através da combinação de técnicas fisiológicas,
bioquímicas e moleculares num briófito proveniente de um habitat que normalmente não
está sujeito à dessecação, comparando com briófitos já estudados e descritos como
tolerantes à dessecação. Além disso, este estudo integrado pretende identificar
processos/mecanismos de DT que sejam comuns nas diversas espécies vegetais tolerantes
à dessecação.
A fotossíntese é um indicador muito sensível à dessecação. A resposta fotossintética
foi seguida por meio de técnicas não-invasivas através da medição da taxa de produção de
oxigénio, acoplado com a fluorescência da clorofila a (Capítulo 2), do briófito F. antipyretica
de modo a determinar o grau de DT. Examinou-se a resposta metabólica à dessecação e
de que forma ocorre a recuperação, se é determinada principalmente pela extensão ou a
taxa de desidratação, ou por ambas. Os nossos dados mostraram que a resposta metabólica
de F. antipyretica à dessecação, tanto sob condições de campo como em laboratório, é
consistente com um padrão de DT. No entanto, a desidratação deve desenrolar-se
lentamente para o briófito recuperar totalmente o seu estado inicial após reidratação. Isto foi
confirmado em amostras dessecadas no campo as quais possuem um padrão de
recuperação semelhante ao das amostras desidratadas lentamente. Este estudo fisiológico
de um briófito aquático com distribuição global contribuiu para melhorar o nosso
conhecimento sobre o papel desempenhado pela taxa de dessecação na sobrevivência dos
briófitos.Durante a desidratação, o normal funcionamento metabólico fica comprometido,
originando um aumento do stresse oxidativo, especialmente na produção de espécies
reativas de oxigénio (ROS, do inglês reactive oxygen species) (Capítulo 3). Desta forma, a
manutenção de níveis aceitáveis de stresse oxidativo que permitam o funcionamento celular
é um aspeto crítico na sobrevivência à dessecação. Embora a produção de ROS em
resposta à dessecação / reidratação tenha sido observada em briófitos terrestres, o mesmo
não sucedeu em briófitos aquáticos. Além disso, não existiam estudos publicados que
relacionassem o impacto da taxa de desidratação na produção de ROS em briófitos
previamente submetidos a dessecação. Uma vez que a taxa de desidratação é fundamental
para sobreviver à dessecação, investigámos se essa resposta incluiu um burst oxidativo
sensível a taxa de desidratação utilizando uma abordagem inovadora que combina sondas
específicas para ROS e microscopia confocal. Após reidratação, observou-se uma elevada
produção de ROS em amostras desidratadas rapidamente, enquanto nas desidratadas
lentamente essa produção era quase ausente.
As ROS reagem com componentes celulares, tais como proteínas e lípidos, levando
a danos celulares, afetando, desta forma, a sobrevivência celular. Depois de observar o
efeito da velocidade de desidratação na produção de ROS, o passo seguinte foi testar como
a taxa de desidratação afetava a estrutura e a estabilidade membranares (Capítulo 4).
Através da quantificação de peroxidação lipídica, da dinâmica de catiões e das medições de
condutividade, foram avaliados os danos e permeabilidade membranares. Dado que o óxido
nítrico também foi associado com DT, tendo um possível papel na proteção da membrana,
procedemos à sua quantificação e discutimos os possíveis papéis da sua produção e ação.
Após a reidratação, observou-se um aumento de ROS e da autofluorescência vermelha os
quais podem ser usados em futuros trabalhos na dessecação e stresse hídrico como um
indicador de danos e rutura da membrana celular.
Durante a desidratação, as relações hídricas das células alteram-se ocorrendo, em
alguns casos, ajustamento osmótico através da acumulação de sacarose. A sacarose tem
um papel importante na DT, Procedeu-se à investigação de como duas taxas de
desidratação contrastantes (rápida e lenta) alteram as relações hídricas e o teor de sacarose
das células de briófitos aquáticos e se essas alterações podem explicar a necessidade de
desidratação lenta para induzir DT (Capítulo 5). As características das paredes celulares
briófitos parecem sofrer alterações durante a desidratação rápida, permitindo à parede
tornar-se mais elástica e que provavelmente permite a manutenção de um metabolismo
funcional até potenciais hídricos mais reduzidos. O aumento de sacarose durante a
desidratação rápida poderá ser uma tentativa de minimizar os danos quando não existe
tempo para estabelecer uma proteção mais efetiva.
A teoria de DT foi baseada num mecanismo de proteção constitutiva que permitiria
ao briófito proteger as estruturas celulares durante a dessecação, juntamente com um mecanismo baseado em reparação após reidratação, que permitiria reparar os danos que
se acumularam durante o estado de dessecação. O controlo de síntese proteica seria
efetuado ao nível transcripcional através da acumulação de mRNA sendo a síntese de
proteínas ativada durante a reidratação. No entanto, recentemente, o estudo de proteomas
em briófitos em resposta à desidratação sugere uma nova abordagem para a DT. Procedeuse
à investigação do efeito de taxas de desidratação lenta e rápida para observar os perfis
proteicos, considerando ambos os processos de desidratação e reidratação (Capítulo 6).
Após a desidratação rápida, os perfis de proteoma de F. antipyretica são muito semelhantes
aos do controlo sem stresse. No entanto, aquando da reidratação após desidratação rápida
observou-se a perda quase total das proteínas, fornecendo evidências de que o briófito não
tem tempo suficiente para se preparar para a dessecação sob esta taxa de desidratação.
Contudo, sob desidratação lenta ocorrem alterações substanciais nos perfis de proteoma,
tanto durante a desidratação como na reidratação, o que pode indicar uma indução de
mecanismos de DT nestas circunstâncias. O desenvolvimento de mecanismos de tolerância
à dessecação, envolvendo a acumulação de proteínas específicas durante os ciclos de
desidratação/reidratação, poderá ter permitido a colonização do meio terrestre pelos
primeiros briófitos. A base para tal tolerância depende de padrões comuns de expressão de
proteínas, bem como de ajustes metabólicos, os quais são muito semelhantes em briófitos
de habitats distintos. Durante a desidratação, a fotossíntese é interrompida, ocorre o
aumento dos níveis de açúcares solúveis, aumentam as proteínas de defesa, o citoesqueleto
é desmontado e enzimas do metabolismo glicolítico aumentam. Após a reidratação, a
fotossíntese reinicia, o citoesqueleto é remontado, e são mantidos elevados níveis de
açúcares solúveis, enzimas do metabolismo glicolítico e proteínas de defesa.
A principal conclusão sugerida por este trabalho é de que a DT ao nível celular,
nomeadamente ao nível dos mecanismos moleculares, é semelhante nos diferentes briófitos
independentemente do seu habitat. Além disso, estabelece que a DT é induzida por
desidratação lenta sendo esta controlada até certo ponto pela morfologia do briófito,
tornando-se um fator determinante na adaptação dos briófitos a cada habitat e às condições
de dessecação.Fundação para a Ciência e Tecnologia (FCT, SFRH/BD/31424/2006
All for One: The Role of Colony Morphology in Bryophyte Desiccation Tolerance
In the last decade, several works showed that even bryophytes from aquatic environments, if slowly dehydrated, can cope with desiccation in a response like the one from desert bryophytes. This led to the hypothesis that, if bryophytes from contrasting habitats can have similar responses, desiccation tolerance (DT) is partially inductive and not only constitutive as previously proposed and, therefore, colony morphology might be the key trait responsible for controlling dehydration rate essential for DT induction. Morphology and life form may be determinant traits in the adaptation of bryophytes to habitats with different water availabilities and corresponding predicted levels in the DT inducibility spectrum. Bryophytes from habitats with different water availabilities were dried as individual shoots and as a colony. The bryophyte Fontinalis antipyretica is fully aquatic presenting a streamer life form, while the three terrestrial species present turf life form with different sizes and degrees of space between individuals in the colony. Two species were collected under trees with moist soil presenting short turf (Tortella tortuosa) and long turf (Campylopus pyriformis) life forms. Another species was completely exposed to sun light with no surrounding trees and a tall turf life form (Pleurochaete squarrosa). We used chlorophyll a fluorescence parameter Fv/Fm (maximum potential quantum efficiency of Photosystem II) as a proxy to photosynthetic fitness throughout the contrasting dehydration rates (fast and slow). These bryophytes with different life forms were submitted to an X-ray computed microtomography (µ-XCT) to assess the three-dimensional inner structure and visualize locations for water storage. Shoots dried slow or fast according to the dehydration they were exposed to, as expected, but they presented similar dehydration rates across different species. However, the aquatic moss F. antipyretica, was unable to recover from fast drying, and after 24 h the recovery following slow drying was lower than the other species. The other three species presented full recovery after 24 h, either at the individual or colony level, and either from slow or fast drying. The only exception was the colonies of Campylopus pyriformis following fast drying that presented a slightly lower recovery, probably due to a looser colony structure.info:eu-repo/semantics/publishedVersio
The use of chitosan oligosaccharide to improve artemisinin yield in well-watered and drought-stressed plants
IntroductionArtemisinin is a secondary metabolite well-known for its use in the treatment of malaria. It also displays other antimicrobial activities which further increase its interest. At present, Artemisia annua is the sole commercial source of the substance, and its production is limited, leading to a global deficit in supply. Furthermore, the cultivation of A. annua is being threatened by climate change. Specifically, drought stress is a major concern for plant development and productivity, but, on the other hand, moderate stress levels can elicit the production of secondary metabolites, with a putative synergistic interaction with elicitors such as chitosan oligosaccharides (COS). Therefore, the development of strategies to increase yield has prompted much interest. With this aim, the effects on artemisinin production under drought stress and treatment with COS, as well as physiological changes in A. annua plants are presented in this study.MethodsPlants were separated into two groups, well-watered (WW) and drought-stressed (DS) plants, and in each group, four concentrations of COS were applied (0, 50,100 and 200 mg•L-1). Afterwards, water stress was imposed by withholding irrigation for 9 days.ResultsTherefore, when A. annua was well watered, COS did not improve plant growth, and the upregulation of antioxidant enzymes hindered the production of artemisinin. On the other hand, during drought stress, COS treatment did not alleviate the decline in growth at any concentration tested. However, higher doses improved the water status since leaf water potential (YL) improved by 50.64% and relative water content (RWC) by 33.84% compared to DS plants without COS treatment. Moreover, the combination of COS and drought stress caused damage to the plant’s antioxidant enzyme defence, particularly APX and GR, and reduced the amount of phenols and flavonoids. This resulted in increased ROS production and enhanced artemisinin content by 34.40% in DS plants treated with 200 mg•L-1 COS, compared to control plants.ConclusionThese findings underscore the critical role of ROS in artemisinin biosynthesis and suggest that COS treatment may boost artemisinin yield in crop production, even under drought conditions
Responsabilidade do Advogado Parecerista em Licitação
The present article aims to analyze the main fundamentals and the positioning of the courts about the responsibility of the public official who acts as referee in public procurements. In other words, the aim is an analysis of current decisions of the Court of Auditors of the Union and of the Supreme Court regarding the accountability of public advocate when issuing legal opinion concerning any hiring process in the public sector. In this way, will address some constitutional aspects that give the competence of members to this exercise carefully the general norms of law and the Attorney General's Office. This analysis will be examinated using as a basis the 1988 Federal Constitution, decisions of the Court of Auditors of the Union Decisions by the Supreme Court and doctrine.O presente artigo tem por objetivo analisar os principais fundamentos e posicionamentos das Cortes quando responsabilizando o agente público que atua como parecerista em contratações públicas. Ou seja, pretende-se uma análise das decisões atuais do Tribunal de Contas da União e do Supremo Tribunal Federal quanto a responsabilização do advogado público quando este emite parecer jurídico referente a algum processo de contratação com a Administração Pública. Neste caminho, serão abordados alguns aspectos constitucionais que conferem a competência dos integrantes para este exercício, com atenção aos normativos gerais da advocacia e da Advocacia Geral da União. Tal análise será examinada tomando por base a Constituição Federal de 1988, as decisões do Tribunal de Contas da União, e Jurisprudência
Herbicide Resistance in Brazil: Status, Impacts, and Future Challenges
Brazil is a large producer and exporter of crops in global terms. Weeds may be responsible for ~14% of crop losses, depending on the crop system. Herbicides occupy 58% of the Brazilian pesticide market; however, the continuous use of these products and the high selection pressure have led to the emergence of weeds resistant to herbicides. Today, there are 51 weed species reported as being resistant to herbicides in Brazil, of which 17 involves cross and multiple-resistance. Acetolactate synthase (ALS), acetyl coenzyme A carboxylase (ACCase) and 5-enolpiruvylshikimate-3-phosphate synthase (EPSPs) inhibitors are the herbicidal groups with the most resistance cases. Soybean, corn, rice, wheat and cotton present 30, 12, 10, 9 and 8 cases, respectively, occurring mainly in herbicide-resistant crop fields from the Southern and Central West regions of the country. To better understand the dimensions of herbicide resistance, in this chapter, we will explore the size of agricultural activity in Brazil, the pesticide market and the use of herbicides in the main crops. In addition, the agronomic, scientific-technical and economic aspects that have contributed, directly or indirectly, to the selection of resistant weeds will be discussed in order to have an overview of the economic impact of herbicide resistance management
Rinoficomicose em Muar
An ulcerated tumour, surgically removed from the Ieft nostril of a mule native or the Rio de Janeiro State, Brazil, was fixed in formol and stained by the Crocott-Gomori, PAS and H.E. methods. The clinical symptoms presented by the mule and the anatomopathological examination results of the collected material made it possible to diagnose, though the etiological agent was not isolated, that it was a case of rhynophycomycosis. Data about phycomycosis forms (mucormycosis, subcutaneous phycomycosis, rhynophycomycosis and hyphomycosis) are given for differential diagnosis. This seems to be the second case of this disease in a mule found in Brazil.Da narina esquerda de um muar procedente do Estado do Rio de Janeiro foi removida cirurgicamente uma lesão granulomatosa ulcerada; esse material foi fixado em formol e corado pelos métodos Grocott-Gomori, PAS e hematoxilina-eosina. O quadro clínico apresentado pelo animal e o resultado do exame anátomo-patológico do material coletado permitiram diagnosticar, sem isolamento do agente etiológico atuante, que se tratava de um caso de rinoficomicose (ficomicose na narina). São fornecidos dados para a diferenciação das formas de ficomicose (mucormicose, ficomicose subcutânea, rinoficomicose) no homem e nos animais e considerações sobre hifomicose nos equídeos. Parece tratar-se do segundo caso de rinoficomicose em muar descrito no Brasil
Are biocrusts and xerophytic vegetation a viable green roof typology in a Mediterranean climate? A comparison between differently vegetated green roofs in water runoff and water quality
Green roofs can be an innovative and effective way of mitigating the environmental impact
of urbanization by providing several important ecosystem services. However, it is known that
the performance of green roofs varies depending on the type of vegetation and, in drier climates,
without resorting to irrigation, these are limited to xerophytic plant species and biocrusts. The aim
of this research was therefore to compare differently vegetated green roofs planted with this type
of vegetation. A particular focus was their ability to hold water during intense stormwater events
and also the quality of the harvested rainwater. Six test beds with different vegetation compositions
were used on the roof of a building in Lisbon. Regarding stormwater retention, the results varied
depending on the composition of the vegetation and the season. As for water quality, almost all the
parameters tested were higher than the Drinking Water Directive from the European Union (EU)
andWord Health Organization (WHO) guidelines for drinking-water quality standards for potable
water. Based on our results, biocrusts and xerophytic vegetation are a viable green roof typology for
slowing runoff during stormwater eventsinfo:eu-repo/semantics/publishedVersio
Developing Emotional Intelligence with a Game: The League of Emotions Learners Approach
Being able to understand, express, and communicate emotions is widely recognized as a fundamental competence. For the younger generation entering the professional market, this is particularly relevant as, in this context, emotions are managed and communicated in ways (and channels) that are different from what they are used to and that can easily lead to misunderstandings. Therefore, it is important to analyze how young people deal with, understand, and interpret emotions, particularly in the context of a professional career where the ability to dialogue with different people and how to get around problems in a healthy and resilient way is essential. This analysis will allow one to design and create tools that allow the younger generation to improve their emotional intelligence and competence. This article introduces the League of Emotions Learners (LoEL) project, an innovative initiative that, through a game app, develops the emotional competence and intelligence of young people. The article then presents the results obtained in the initial validation that led to the positive understanding of its impact.This research was partly funded by the European Union, through the Erasmus+ programme, KA2 action, under the grant number 2018-1-ES02-KA205-011836.info:eu-repo/semantics/publishedVersio
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