Metabolic Diseases: a differential diagnosis of primary progressive multiple sclerosis

Objectives: The overall aim of our research project is to develop a Next Generation Sequencing strategy to identify metabolic disorders in 104 patients with a presumptive diagnosis of primary progressive MS.We would like to thank to MERCK, SA and NORTE2020 (NORTE-01-0246-FEDER-000014) for funding this Project.N/

Weaning of Senegalese sole (Solea senegalensis) postlarvae to an inert diet with a co-feeding regime | Destete de postlarvas del lenguado senegalés (Solea senegalensis) a una dieta inerte utilizando un régimen de coalimentación

The objective of this study was to evaluate the weaning success of Senegalese sole, Solea senegalensis, postlarvae when cofed a compound diet, by means of the analysis of growth parameters (relative growth rate [RGR] and condition factor [K]), survival, protein and lipid content. Total ammonia in the rearing water was also measured. The experiment lasted for 46 days (36 to 82 days posthatching). One group was fed only with enriched Artemia sp. metanauplii (Artemia treatment), whereas in another group, the Artemia sp. metanauplii were gradually replaced by the International Council for the Exploration of the Sea (ICES) diet over a period of 39 days (ICES treatment). Postlarvae were sampled on rearing days 0, 9, 23, 30, 39 and 46 for growth and biochemical analysis. Water was sampled on rearing day 30 during a 24-h cycle in both treatments. Postlarvae from the ICES treatment were weaned, though by the end of the experiment the Artemia treatment exhibited significantly higher values for growth, RGR, K and survival rates (P <0.05). No significant differences were observed concerning total lipid content. Protein content was significantly higher for the Artemia treatment after 46 days of rearing (P < 0.05). The total ammonia nitrogen concentration in the water increased after each meal and remained below a harmless level for postlarvae.El objetivo de este estudio consistió en evaluar el éxito del destete de postlarvas del lenguado senegalés, Solea senegalensis, utilizando un régimen de coalimentación. El estudio se realizó mediante el análisis de parámetros de crecimiento (tasa de crecimiento relativa [RGR] y factor de condición [K]), supervivencia y contenido de proteínas y lípidos. La concentración del amonio total en el agua de cultivo también fue analizada. El experimento se realizó durante 46 días (de 36 a 82 días posteclosión). Un grupo de postlarvas se alimentó únicamente con metanauplios de Artemia sp. enriquecidos (tratamiento con Artemia), mientras que en otro grupo los metanauplius de Artemia sp. fueron gradualmente substituidos por la dieta del International Council for the Exploration of the Sea (tratamiento ICES) durante un periodo de 39 días. Las postlarvas se muestrearon durante los días 0, 9, 23, 30, 39 y 46 de cultivo para el estudio de crecimiento y el análisis bioquímico Las muestras de agua se tomaron el día 30 de cultivo durante un ciclo de 24 h en ambos tratamientos. Las postlarvas del tratamiento ICES fueron destetadas, aunque al final del experimento se registraron valores significativamente mayores en relación al crecimiento, RGR, K y tasas de supervivencia (P < 0.05) en el tratamiento con Artemia. No se observaron diferencias significativas en el contenido total de lípidos. El contenido de proteínas fue significativamente mayor en el tratamiento con Artemia a los 46 días de cultivo (P < 0.05). La concentracion de amonio total en el agua se incrementó después de cada toma de alimento pero se mantuvo por debajo del nivel tóxico para las postlarvas de peces marinos

Aplicação do Benchmarking na indústria de manufactura : Desenvolvimento de uma metodologia para empresas de fundição

Tese de doutoramento. Faculdade de Engenharia. Universidade do Porto. 199

COVID-19 impact on the assessment methodology of undergraduate medical students: a systematic review of the lessons learned

The COVID-19 pandemic had a substantial social, economic, political, and educational impact worldwide. Due to the social contact restrictions, areas such as medical education were highly affected. Assessment in medical education, was already a sensitive topic, and it proved to be even more challenging as different teaching and learning contexts required huge adaptations in a short period. This systematic review provides an overview of the impact of COVID-19 on the assessment of medical students and can serve as a reference to improve this area. We conducted the review based on the PRISMA tool and searched in PubMed, EBSCO, and ScienceDirect. Studies describing the assessment methodologies used during the pandemic were included. Of the 501 initial articles, 18 were included in this review. Collected data was based on the regime, subject, teaching/assessment methodologies, platforms used, grades, students’ and teachers’ perceptions, and measures to prevent academic dishonesty. The results suggest that technology played a central role during the pandemic, and universities were concerned about the transition to online learning regarding teaching and assessment, but students and teachers should be prepared for it. Formative assessment methodologies and feedback were emphasized, and summative tools were adapted to prevent fraud. Students and teachers were generally satisfied with online learning and assessment, which had no significant difference in the examination scores, but they preferred conventional teaching. The COVID-19 pandemic brought an opportunity to analyze and rethink the medical curriculum. Thus, further investigations are needed on combining traditional and online teaching strategies and emphasis on the assessment

Análises comparativas entre textos jurídicos espanhóis e portugueses

Apesar da importância reconhecida à textologia contrastiva na didática da tradução, são extremamente escassos os estudos focados no par de línguas espanhol-português. Como forma de compensar, pelo menos em parte, essa enorme lacuna, propôs-se aos alunos de Tradução Jurídica de Espanhol do MTIE do ISCAP um trabalho de análise de diversos textos jurídicos autênticos. Três desses trabalhos são aqui apresentados, os quais abordam tipos de texto bem diferenciados: os recursos de apelação, os testamentos e os contratos de trabalho.Despite the importance acknowledged to contrastive textology for translation teaching, studies focusing on the Spanish-Portuguese language pair are extremely scarce. As a way of making up, at least partially, for this huge gap, the students enrolled in Spanish-Portuguese Legal Translation of the ISCAP master's programme were challenged to analyse and contrast authentic legal texts. Three of those essays are here included, dealing with three very different types of text: appeals, wills and employment contracts

Understanding fish larvae’s feeding biology to improve aquaculture feeding protocols

Knowing that food ingestion and digestion are processes under neuroendocrine regulation, it is important to understand how fish larvae regulate these processes, when the digestive system itself is under development. This study aimed to analyze how gilthead seabream (Sparus aurata) larval feeding incidence and intensity were affected by time, light, and water temperature, through the analysis of gut content and how this affected cholecystokinin (CCK) content. Three short-term experiments were done: (A) 27 and 42 days post-hatch (DPH) larvae were analyzed at different intervals for 80 min, after feeding; (B) 38 and 48 DPH larvae were kept under different light intensities (0, 400, 1000 lux) for 30 min; (C) 41 DPH larvae were kept at 17, 19, 25 °C for 30 min. Feeding incidence below 100% was observed for 27 DPH larvae, and for 38 DPH larvae fed at 0 lux. Feeding intensity was several fold higher at older ages. However, the number of prey in the gut was lower at 0 lux, and higher at 25 °C. Overall, no clear pattern was observed for CCK, but at older ages increasing gut content resulted in higher CCK content. In conclusion, until 38 DPH light is important for gilthead sea bream larval feeding ability, and maturing functionality of CCK was only observed at later stages.info:eu-repo/semantics/publishedVersio

Microplastics effects in Scrobicularia plana

One of the most common plastics in the marine environment is polystyrene (PS) that can be broken down to micro sized particles. Marine organisms are vulnerable to the exposure to microplastics. This study assesses the effects of PS microplastics in tissues of the clam Scrobicularia plana. Clams were exposed to 1mgL-1(20μm) for 14days, followed by 7days of depuration. A qualitative analysis by infrared spectroscopy in diffuse reflectance mode period detected the presence of microplastics in clam tissues upon exposure, which were not eliminated after depuration. The effects of microplastics were assessed by a battery of biomarkers and results revealed that microplastics induce effects on antioxidant capacity, DNA damage, neurotoxicity and oxidative damage. S. plana is a significant target to assess the environmental risk of PS microplastics.info:eu-repo/semantics/publishedVersio