733 research outputs found
Transplantation of Human Neuroblastoma Cells, Catecholaminergic and Non-Catecholaminergic: Effects on Rotational Behavoir in Parkinson's Rat Model
Cultured human catecholaminergic and noncatecholaminergic
donor cells were used in
neural transplantation experiments in a rat
model of Parkinson's disease. Using two different
human catecholaminergic neuroblastoma cell
lines, one control non-catecholaminergic neuroblastoma
cell line, and one sham control (tissue
culture medium), transplants were made into the
striatum using a modified Ungerstedt
hemiparkinsonian rat model. Significant
decreases in apomorphine-induced rotational
behavior were produced by two of three
catecholaminergic cell lines. Grafted cells staining
positively for tyrosine hydroxylase (TH) and
catecholamine fluorescence indicated viable catecholamine
activity in the two cell lines which produced
reductions in rotational behavior.
Catecholamine fluorescence was not detected in
either of the two controls. These data suggest a
link between catecholamine secretion by transplanted
cells and motor improvement using a rat
rotational behavior model
Observations of X-rays and Thermal Dust Emission from the Supernova Remnant Kes 75
We present Spitzer Space Telescope and Chandra X-ray Observatory observations
of the composite Galactic supernova remnant Kes 75 (G29.7-0.3). We use the
detected flux at 24 microns and hot gas parameters from fitting spectra from
new, deep X-ray observations to constrain models of dust emission, obtaining a
dust-to-gas mass ratio M_dust/M_gas ~0.001. We find that a two-component
thermal model, nominally representing shocked swept-up interstellar or
circumstellar material and reverse-shocked ejecta, adequately fits the X-ray
spectrum, albeit with somewhat high implied densities for both components. We
surmise that this model implies a Wolf-Rayet progenitor for the remnant. We
also present infrared flux upper limits for the central pulsar wind nebula.Comment: 7 pages, 2 tables, 4 figures, uses emulateapj. Accepted for
publication in Ap
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines
Neuroblastoma cell lines are an important and cost-effective model used to study oncogenic drivers of the disease. While many of these cell lines have been previously characterized with SNP, methylation, and/or mRNA expression microarrays, there has not been an effort to comprehensively sequence these cell lines. Here, we present raw whole transcriptome data generated by RNA sequencing of 39 commonly-used neuroblastoma cell lines. These data can be used to perform differential expression analysis based on a genetic aberration or phenotype in neuroblastoma (e.g., MYCN amplification status, ALK mutation status, chromosome arm 1p, 11q and/or 17q status, sensitivity to pharmacologic perturbation). Additionally, we designed this experiment to enable structural variant and/or long-noncoding RNA analysis across these cell lines. Finally, as more DNase/ATAC and histone/transcription factor ChIP sequencing is performed in these cell lines, our RNA-Seq data will be an important complement to inform transcriptional targets as well as regulatory (enhancer or repressor) elements in neuroblastoma
Ionization structure and Fe K energy for irradiated accretion disks
We study the radial ionization structure at the surface of an X-ray
illuminated accretion disk. We plot the expected iron K line energy as
a function of the Eddington ratio and of the distance of the emitting matter
from the central source, for a non-rotating and a maximally-rotating black
hole. We compare the predicted disk line energies with those measured in an
archival sample of active galactic nuclei observed with {\it Chandra}, {\it
XMM-Newton} and {\it Suzaku}, and discuss whether the line energies are
consistent with the radial distances inferred from reverberation studies. We
also suggest using rapidly-variable iron K lines to estimate the
viscosity parameter of an accretion disk. There is a forbidden region in the
line energy versus Eddington ratio plane, at low Eddington ratios, where an
accretion disk cannot produce highly-ionized iron K lines. If such
emission is observed in low-Eddington-ratio sources, it is either coming from a
highly-ionized outflow, or is a blue-shifted component from fast-moving neutral
matter.Comment: 5 pages, 2 figures, accepted by MNRA
Создание монумента «Возрождение крымскотатарского народа» как культурное явление в жизни крымского общества
В статье автором охарактеризован градостроительный объект Монумент «Возрождение крымскотатарского народа» как культурное явление в жизни крымского общества, призванное внести вклад в процесс воспитания духовности и культуры в молодых людях.У статті автором охарактеризовано містобудівельний об’єкт Монумент «Відродження кримськотатарського народу» як культурне явище у житті кримського суспільства, що призвание донести внесок у процес виховання духовності та культури молоді.The author describes a monument „Rebirth of the Crimean Tatars” as a cultural phenomenon in the life of the Crimean society, which can contribute in the process of spiritual and cultural upbringing of young people
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Cultural Heritage and the Campus Community: Academic Libraries and Museums in Collaboration
This chapter describes the broad set of collaborations that have existed for nearly a decade between the University Libraries and the Natural History Museum at the University of Colorado, Boulder. This collabo- rative partnership focuses on areas of collection management, access, user engagement, and programming as well as joint-use facility devel- opment. Continued program, accessibility, stewardship, and shared facility development will build on the sturdy foundations of shared opportunities and challenges that have defined current high levels of collaborations between these two CU-Boulder campus units.</p
Quasi-Periodic Occultation by a Precessing Accretion Disk and Other Variabilities of SMC X-1
We have investigated the variability of the binary X-ray pulsar, SMC X-1, in
data from several X-ray observatories. We confirm the ~60-day cyclic variation
of the X-ray flux in the long-term monitoring data from the RXTE and CGRO
observatories. X-ray light curves and spectra from the ROSAT, Ginga, and ASCA
observatories show that the uneclipsed flux varies by as much as a factor of
twenty between a high-flux state when 0.71 second pulses are present and a
low-flux state when pulses are absent. In contrast, during eclipses when the
X-rays consist of radiation scattered from circumsource matter, the fluxes and
spectra in the high and low states are approximately the same. These
observations prove that the low state of SMC X-1 is not caused by a reduction
in the intrinsic luminosity of the source, or a spectral redistribution
thereof, but rather by a quasi-periodic blockage of the line of sight, most
likely by a precessing tilted accretion disk. In each of two observations in
the midst of low states a brief increase in the X-ray flux and reappearance of
0.71 second pulses occurred near orbital phase 0.2. These brief increases
result from an opening of the line of sight to the pulsar that may be caused by
wobble in the precessing accretion disk. The records of spin up of the neutron
star and decay of the binary orbit are extended during 1991-1996 by
pulse-timing analysis of ROSAT, ASCA, and RXTE PCA data. The pulse profiles in
various energy ranges from 0.1 to >21 keV are well represented as a combination
of a pencil beam and a fan beam. Finally, there is a marked difference between
the power spectra of random fluctuations in the high-state data from the RXTE
PCA below and above 3.4 keV. Deviation from the fitted power law around 0.06 Hz
may be QPO.Comment: Accepted to ApJ. 33 pages including 11 figure
Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell–mediated liver damage
Hepatitis B virus (HBV) causes chronic infection in more than 350 million people worldwide. It replicates in hepatocytes but is non-cytopathic; liver damage is thought to be immune mediated. Here, we investigated the role of innate immune responses in mediating liver damage in patients with chronic HBV infection. Longitudinal analysis revealed a temporal correlation between flares of liver inflammation and fluctuations in interleukin (IL)-8, interferon (IFN)-α, and natural killer (NK) cell expression of tumor necrosis factor–related apoptosis-inducing ligand (TRAIL) directly ex vivo. A cross-sectional study confirmed these findings in patients with HBV-related liver inflammation compared with healthy carriers. Activated, TRAIL-expressing NK cells were further enriched in the liver of patients with chronic HBV infection, while their hepatocytes expressed increased levels of a TRAIL death–inducing receptor. IFN-α concentrations found in patients were capable of activating NK cells to induce TRAIL-mediated hepatocyte apoptosis in vitro. The pathogenic potential of this pathway could be further enhanced by the ability of the IFN-α/IL-8 combination to dysregulate the balance of death-inducing and regulatory TRAIL receptors expressed on hepatocytes. We conclude that NK cells may contribute to liver inflammation by TRAIL-mediated death of hepatocytes and demonstrate that this non-antigen–specific mechanism can be switched on by cytokines produced during active HBV infection
Impact of Solitary Involved Lymph Node on Outcome in Localized Cancer of the Esophagus and Esophagogastric Junction
Node-positive esophageal cancer is associated with a dismal prognosis. The impact of a solitary involved node, however, is unclear, and this study examined the implications of a solitary node compared with greater nodal involvement and node-negative disease. The clinical and pathologic details of 604 patients were entered prospectively into a database from1993 and 2005. Four pathologic groups were analyzed: node-negative, one lymph node positive, two or three lymph nodes positive, and greater than three lymph nodes positive. Three hundred and fifteen patients (52%) were node-positive and 289 were node-negative. The median survival was 26 months in the node-negative group. Patients (n = 84) who had one node positive had a median survival of 16 months (p = 0.03 vs node-negative). Eighty-four patients who had two or three nodes positive had a median survival of 11 months compared with a median survival of 8 months in the 146 patients who had greater than three nodes positive (p = 0.01). The survival of patients with one node positive [number of nodes (N) = 1] was also significantly greater than the survival of patients with 2–3 nodes positive (N = 2–3) (p = 0.049) and greater than three nodes positive (p < 0001). The presence of a solitary involved lymph node has a negative impact on survival compared with node-negative disease, but it is associated with significantly improved overall survival compared with all other nodal groups
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