158 research outputs found
Sur lâexistence en France de lâhĂ©patite Ă virus caneton
Guillon J.-C., Renault Lucien. Sur l'existence en France de l'hépatite à virus du caneton. In: Bulletin de l'Académie Vétérinaire de France tome 113 n°4, 1960. pp. 237-243
Apparition dâhĂ©patomes chez des canes
Guillon J.-C., Renault Lucien. Apparition dâhĂ©patomes chez des canes. In: Bulletin de l'AcadĂ©mie VĂ©tĂ©rinaire de France tome 114 n°2, 1961. pp. 94-97
Tiling groupoids and Bratteli diagrams
Let T be an aperiodic and repetitive tiling of R^d with finite local
complexity. Let O be its tiling space with canonical transversal X. The tiling
equivalence relation R_X is the set of pairs of tilings in X which are
translates of each others, with a certain (etale) topology. In this paper R_X
is reconstructed as a generalized "tail equivalence" on a Bratteli diagram,
with its standard AF-relation as a subequivalence relation.
Using a generalization of the Anderson-Putnam complex, O is identified with
the inverse limit of a sequence of finite CW-complexes. A Bratteli diagram B is
built from this sequence, and its set of infinite paths dB is homeomorphic to
X. The diagram B is endowed with a horizontal structure: additional edges that
encode the adjacencies of patches in T. This allows to define an etale
equivalence relation R_B on dB which is homeomorphic to R_X, and contains the
AF-relation of "tail equivalence".Comment: 34 pages, 4 figure
Control of membrane barrier during bacterial type-III protein secretion
Type-III secretion systems (T3SSs) of the bacterial flagellum and the evolutionarily related injectisome are capable of translocating proteins with a remarkable speed of several thousand amino acids per second. Here, we investigate how T3SSs are able to transport proteins at such a high rate while preventing the leakage of small molecules. Our mutational and evolutionary analyses demonstrate that an ensemble of conserved methionine residues at the cytoplasmic side of the T3SS channel create a deformable gasket (M-gasket) around fast-moving substrates undergoing export. The unique physicochemical features of the M-gasket are crucial to preserve the membrane barrier, to accommodate local conformational changes during active secretion, and to maintain stability of the secretion pore in cooperation with a plug domain (R-plug) and a network of salt-bridges. The conservation of the M-gasket, R-plug, and salt-bridge network suggests a universal mechanism by which the membrane integrity is maintained during high-speed protein translocation in all T3SSs.Peer Reviewe
CMG-Pol epsilon dynamics suggests a mechanism for the establishment of leading-strand synthesis in the eukaryotic replisome
The replisome unwinds and synthesizes DNA for genome duplication. In eukaryotes, the Cdc45âMCMâGINS (CMG) helicase and the leading-strand polymerase, Pol epsilon, form a stable assembly. The mechanism for coupling DNA unwinding with synthesis is starting to be elucidated, however the architecture and dynamics of the replication fork remain only partially understood, preventing a molecular understanding of chromosome replication. To address this issue, we conducted a systematic single-particle EM study on multiple permutations of the reconstituted CMGâPol epsilon assembly. Pol epsilon contains two flexibly tethered lobes. The noncatalytic lobe is anchored to the motor of the helicase, whereas the polymerization domain extends toward the side of the helicase. We observe two alternate configurations of the DNA synthesis domain in the CMG-bound Pol epsilon. We propose that this conformational switch might control DNA template engagement and release, modulating replisome progression.FWN â Publicaties zonder aanstelling Universiteit Leide
Structure, electrochemical properties and functionalization of amorphous CN films deposited by femtosecond pulsed laser ablation
Amorphous carbon nitride (a-C:N) material has attracted much attention in research and development. Recently, it has become a more promising electrode material than conventional carbon based electrodes in electrochemical and biosensor applications. Nitrogen containing amorphous carbon (a-C:N) thin films have been synthesized by femtosecond pulsed laser deposition (fs-PLD) coupled with plasma assistance through Direct Current (DC) bias power supply. During the deposition process, various nitrogen pressures (0 to 10 Pa) and DC bias (0 to Âż 350 V) were used in order to explore a wide range of nitrogen content into the films. The structure and chemical composition of the films have been studied by using Raman spectroscopy, electron energy-loss spectroscopy (EELS) and high-resolution transmission electron microscopy (HRTEM). Increasing the nitrogen pressure or adding a DC bias induced an increase of the N content, up to 21 at.%. Nitrogen content increase induces a higher sp2 character of the film. However DC bias has been found to increase the film structural disorder, which was detrimental to the electrochemical properties. Indeed the electrochemical measurements, investigated by cyclic voltammetry (CV), demonstrated that a-C:N film with moderate nitrogen content (10 at.%) exhibited the best behavior, in terms of reversibility and electron transfer kinetics. Electrochemical grafting from diazonium salts was successfully achieved on this film, with a surface coverage of covalently bonded molecules close to the dense packed monolayer of ferrocene molecules. Such a film may be a promising electrode material in electrochemical detection of electroactive pollutants on bare film, and of biopathogen molecules after surface grafting of the specific affinity receptor.This work is produced with the financial support of the Future Program Lyon Saint-Etienne (PALSE) from the University of Lyon (ANR-11-IDEX-0007), under the âInvestissements d'Avenirâ program managed by the National Agency Research (ANR)
The CAT Imaging Telescope for Very-High-Energy Gamma-Ray Astronomy
The CAT (Cherenkov Array at Themis) imaging telescope, equipped with a
very-high-definition camera (546 fast phototubes with 0.12 degrees spacing
surrounded by 54 larger tubes in two guard rings) started operation in Autumn
1996 on the site of the former solar plant Themis (France). Using the
atmospheric Cherenkov technique, it detects and identifies very high energy
gamma-rays in the range 250 GeV to a few tens of TeV. The instrument, which has
detected three sources (Crab nebula, Mrk 421 and Mrk 501), is described in
detail.Comment: 24 pages, 15 figures. submitted to Elsevier Preprin
Azimuthal anisotropy and correlations in p+p, d+Au and Au+Au collisions at 200 GeV
We present the first measurement of directed flow () at RHIC. is
found to be consistent with zero at pseudorapidities from -1.2 to 1.2,
then rises to the level of a couple of percent over the range . The latter observation is similar to data from NA49 if the SPS rapidities
are shifted by the difference in beam rapidity between RHIC and SPS.
Back-to-back jets emitted out-of-plane are found to be suppressed more if
compared to those emitted in-plane, which is consistent with {\it jet
quenching}. Using the scalar product method, we systematically compared
azimuthal correlations from p+p, d+Au and Au+Au collisions. Flow and non-flow
from these three different collision systems are discussed.Comment: Quark Matter 2004 proceeding, 4 pages, 3 figure
- âŠ