Identifikasi Salmonella pullorum pada Ayam Petelur Periode Grower dengan Uji Aglutinasi dan Makroskopik di Peternakan Ayam Kabupaten Sidrap

ABSTRAKPenyakit pullorum merupakan penyakit yang sering menginfeksi ayam yang disebabkan oleh Salmonella pullorum. Penelitian ini bertujuan untuk mengidentifikasi ada tidaknya penyakit pullorum pada ayam petelur periode grower di peternakan ayam kabupaten Sidrap. Pengambilan sampel menggunakan metode purposive sampling. Sampel serum dan organ diambil dari 19 peternakan ayam di Batu-Batu. Sebanyak 57 sampel serum diuji dengan uji aglutinasi. Diperoleh sebanyak 26 sampel serum yang positif dan dilanjutkan dengan pemeriksaan makroskopik. Pemeriksaan makroskopik ditemukan adanya hepatomegali, lesi berwarna kuning-pucat, konsistensi lunak, dan nodul-nodul di permukaan hati serta adanya kardomegali. Hasil penelitian ini menunjukkan bahwa peternakan ayam layer periode grower di kabupaten Sidrap telah terinfeksi Salmonella pullorum dangan gambaran patologis yang bervariasi.  Kata kunci: ayam petelur, identifikasi, Salmonella pullorum, makroskopikIdentification of Salmonella Pullorum in Grower Period of Laying Hens By Agglutinated and Macroscopic Test in Layer Farms of Sidrap RegencyABSTRACTPullorum is a bacterial disease that commonly infected the chicken infected by Salmonella pullorum. This research aimed to identify Salmonella pullorum in the growing period of laying hen in Batu-Batu. The research used a purposive sampling method. The organ and serum samples were collected from 19 farms. Agglutination test for serum and macroscopic test for organ was performed. There were 26 samples that tested positive from 57 serum samples. There were 26 organ samples of macroscopic treatment that resulted in liver damages in the forms of swelling, pale yellow lesions, soft consistency, and nodules on the surface. The heart also experienced changes through swelling, soft consistency, uneven surface, and a mixture of fluid and fibrin inside the pericardium. It can be concluded that layer farms in Sidrap districts have been infected by Salmonella pullorum affecting the growing period.Keywords : laying hens, identification, Salmonella pullorum, macroscopi

Deteksi White Spot Syndrome Virus pada Lobster Menggunakan Primer Kit IQ2000™

Lobster adalah salah satu jenis bahan pangan yang menjadi komoditas ekspor tertinggi. Lobster dapat diinfeksi oleh White Spot Syndrome Virus yang menyebabkan kematian dan kerugian ekonomi. Penelitian ini untuk mendeteksi adanya infeksi White Spot Syndrome Virus pada lobster di Balai Besar Karantina Ikan dan Pengendalian Hasil Mutu Makassar. Sebanyak 3 sampel lobster Panulirus spp diperiksa secara molekular. Deteksi White Spot Syndrome Virus dilakukan dengan menggunakan Primer Kit IQ2000™ pada mesin Polymerase Chain Reaction. Sampel positif terinfeksi White Spot Syndrome Virus ditandai dengan pita pada agar gel yang terbentuk dengan ukuran 296 bp dan 550 bp, sampel negatif jika pita yang terbentuk berada pada 848 bp. Hasil yang diperoleh setelah eletroforesis sampel pada gel agaros 1% ditemukan adanya pita yang berukuran 848 bp yang menunjukan hasil negatif. Berdasarkan hasil pengujian diatas dapat disimpulkan bahwa ketiga sampel lobster yang diperiksa di Balai Besar Karantina Ikan dan Pengendalian Hasil Mutu Makassar tidak terinfeksi White Spot Syndrome Virus. 

Mapping of the Coronavirus Circulating in Asia Based on Sequence of Gene Spike and Membrane Protein Used MEGA-X Aplication

Coronavirus are viruses that can be transmitted to human and animals. Severe acute respiratory syndrome, middle east respiratory syndrome, and Coronavirus disease 2019 are disease can be caused by several subtypes of coronavirus.  The aims of this study were to mapping of the coronavirus circulating in Asia based on sequence of gene spike and membrane protein virus. Totally of 67 coronavirus spike protein and membrane gene sequence were accessed via GenBank® (www.ncbi.nlm.nih.gov/genbank/) matched with the ClustalW Method MEGA-X. The result of the study are 20 groups of coronavirus were found based on spike protein gene sequences and 27 groups of coronavirus were found based on membrane protein gene sequences which were different with the first group of coronavirus found in Wuhan. Therefore, it can be concluded that the coronavirus circulate in several Asian countries had been mutate on gene spike and membrane protein. Keywords: Asia, Coronavirus, MEGA-X, Membrane Protein, Spike Protei

Administration of Live-Attenuated Newcastle Disease (ND) Vaccines Derived from B1 and LaSota Strain and Their Effect on Broiler Antibody Titers

Newcastle Disease is a contagious disease caused by Avian Paramyxovirus and infects different types of poultries including broiler. Vaccination as a preventive effort against the ND virus could employ both attenuated and inactivated vaccines. This study aims to identify the effect of administering live-attenuated LaSota and B1 ND vaccines against broiler antibody titers. This experiment employed 54 DOCs and was divided into 3 groups of treatments. Vaccination was performed at the age of 3 days old through eye drop administration. Blood specimens were taken from vena brachialis at the age of 7, 14, 21 days old. Hemagglutination Inhibition (HI) assay was analyzed using One-Way Analysis of Variance (ANOVA) followed by Least Significant Different (LSD) test if the probability is significant (P<0.05). The data of Immune Percentage is presented using descriptive quantitative analysis.   The research results demonstrated that live-attenuated LaSota ND vaccination at the age of 3 days could sustain and induce immunity until the age of 21 days while B1 ND vaccination at the age of 3 days could only sustain immune protection until the age of 14 days. Live-attenuated LaSota and B1 ND vaccines did not have any significant effect on the broiler antibody titers.   Keywords: Broiler, Newcastle Disease (ND), LaSota vaccine, B1 vaccine, Hemagglutination test, Hemagglutination Inhibition Tes

UJI AKTIVITAS EKSTRAK ETANOL KULIT PISANG MAS (Musa acuminata (AA Goup)) TERHADAP PENURUNAN KADAR GLUKOSA DARAH MENCIT (Mus Musculus) YANG DIINDUKSI ALOKSAN

Diabetes mellitus (DM) is a metabolic diseases caused by impaired insulin secretion. The prevalence of DM in pets increased from 2006 until 2015.Therapy by using insulin feared can be worse for patient, so yhat alternative medicine is needed like banana peel. The aims of this study isto determine the effect ofethanol extract of Mas banana peel (Musa acuminata (AA Group)) to decrease level of glucose in mice’s blood (Mus musculus) were induced by alloxan. This research was conducted in June 2017 at Biopharmaceutical Laboratory Faculty of Pharmacy, Hasanuddin University. This study uses 24 male mice which divided into 6 groups where the normal control (standard diet), negative control (Na.Cmc 1%), positive control (Metformin 140mg/KgBB) and treatment group by ethanol extract of Mas banana peel 1%, 5%, and 25%. The treatment was given for 10 days. Data were analyzed using one way Anova. The result of this study showed that ethanol extract in banana peel decrease the level of blood glucose which the decrease percentage are 43,15%, 54,61%, 74,28%. The summary of this research is the treatment by using variant concentration of ethanol extract of Mas banana peelfor 10 days give the significant effect for decreasing the level of blood glucose (p0,05)

UJI AKTIVITAS EKSTRAK ETANOL KULIT PISANG MAS (Musa acuminata (AA Goup)) TERHADAP PENURUNAN KADAR GLUKOSA DARAH MENCIT (Mus Musculus) YANG DIINDUKSI ALOKSAN

Diabetes mellitus (DM) is a metabolic diseases caused by impaired insulin secretion. The prevalence of DM in pets increased from 2006 until 2015.Therapy by using insulin feared can be worse for patient, so yhat alternative medicine is needed like banana peel. The aims of this study isto determine the effect ofethanol extract of Mas banana peel (Musa acuminata (AA Group)) to decrease level of glucose in mice’s blood (Mus musculus) were induced by alloxan. This research was conducted in June 2017 at Biopharmaceutical Laboratory Faculty of Pharmacy, Hasanuddin University. This study uses 24 male mice which divided into 6 groups where the normal control (standard diet), negative control (Na.Cmc 1%), positive control (Metformin 140mg/KgBB) and treatment group by ethanol extract of Mas banana peel 1%, 5%, and 25%. The treatment was given for 10 days. Data were analyzed using one way Anova. The result of this study showed that ethanol extract in banana peel decrease the level of blood glucose which the decrease percentage are 43,15%, 54,61%, 74,28%. The summary of this research is the treatment by using variant concentration of ethanol extract of Mas banana peelfor 10 days give the significant effect for decreasing the level of blood glucose (p0,05)

Zoonotic : Emerging and Reemerging Viral Diseases in Indonesia

Zoonoses are diseases that have claimed millions of lives in both humans and animals. This disease can be transferred from infected animals to humans or vice versa. It has been reported that 75% of zoonotic diseases are emerging. This paper aims to describe various types of emerging and reemerging zoonotic diseases that occur in Indonesia and abroad. Data collection is done online through several websites including the Office International des Epizooties, World Health Organization and the Ministry of Health of the Republic of Indonesia in collaboration with published research reports related to zoonotic diseases. Various disease data were found, both emerging and reemerging. The zoonotic diseases that are endemic and have been reported include COVID-19, avian influenza, swine influenza, dengue, chikungunya, hepatitis E, and rabies. All these diseases occur in Indonesia and throughout the world. Based on the description above, it can be concluded that various types of emerging and reemerging zoonotic diseases occur in Indonesia

Identification of Yersinia spp in Catfish (Claria spp) Attacked Enteric Redmouth Disease at the Education Veterinary Clinic, Hasanuddin University

Catfish is one of the fish that has a high economic value so that it becomes one of the export commodities to meet the consumption and protein needs of the community so that it is widely cultivated. In cultivation, catfish can be attacked by various diseases, one of which can be caused by bacteria. The bacteria that often cause death in fish, both marine and freshwater fish, is Yersinia spp. Yersinia spp is a group of gram-negative bacteria, in the form of bacilli and motile and is also reported to be one of the pathogenic diseases that often attack fish. samples were taken on August 12, 2021 from the mouth organs of catfish at the Hasanuddin University Education Animal Clinic. Samples were cultured in Tryptic Soy Agar (TSA) for 24 hours and then purified in Mac Conkey Agar (MCA). The culture results showed the presence of bacterial growth. Follow-up examination using a microscope and showed the presence of red (Gram negative) and bacilli-shaped bacteria

Treatment Leptospira Infection In Dog At Pet Vet Clinic, Center of Jakarta

Leptospirosis is a disease caused by Leptospira spp., zoonotic Gram-negative bacteria. This study aimed to determine the handling of a leptospirosis case in a dog at the Pet Vet Clinic, Jakarta. Handling began with anamnesis and continued by examining the patient's physical condition, followed by serological, microscopic, and polymerase chain reaction examinations. An examination revealed that the dog had Leptospirosis. The therapy given was the administration of crystalline procaine and penicillin-G antibiotics. Supportive treatment was given with hepatoprotective, lipotropic, and diuretic supplements. Ringer's lactate was used as fluid therapy. The treatment administered showed positive, progressive results in the patient

African Swine Fever and Its Socio-Economic Impacts in Indonesia

African Swine Fever Virus (ASF) is a viral disease in pigs that affects the supply of food of animal origin.  ASF is caused by a double-stranded DNA virus of the family Asfarviridae and genus Asfivirus, which can be transmitted and causes various bleeding events in the internal organs and is accompanied by a very high mortality rate.  This disease is generally characterized by fever, lack of appetite, and bleeding from the skin, back, and legs.  Even though this virus is not zoonotic and cannot be transmitted to humans, it is fatal and causes tremendous economic loss.  This disease spread throughout Asia in a relatively short time in 2019, and it has even been reported that this disease has entered Indonesia since the end of 2019.  The impact of this infection resulted in socioeconomic changes in both rural and urban communities, which could then affect the country's economy at the macro level.  Several countries are needed in their efforts to control ASF, one of which is to increase quarantine, limit the traffic of pigs, and reduce the population of sick pigs exposed to the ASF virus