Memory Effects in Spontaneous Emission Processes

We consider a quantum-mechanical analysis of spontaneous emission in terms of an effective two-level system with a vacuum decay rate $\Gamma_0$ and transition angular frequency $\omega_A$. Our analysis is in principle exact, even though presented as a numerical solution of the time-evolution including memory effects. The results so obtained are confronted with previous discussions in the literature. In terms of the {\it dimensionless} lifetime $\tau = t\Gamma_0$ of spontaneous emission, we obtain deviations from exponential decay of the form ${\cal O} (1/\tau)$ for the decay amplitude as well as the previously obtained asymptotic behaviors of the form ${\cal O} (1/\tau^2)$ or ${\cal O} (1/\tau \ln^2\tau)$ for $\tau \gg 1$. The actual asymptotic behavior depends on the adopted regularization procedure as well as on the physical parameters at hand. We show that for any reasonable range of $\tau$ and for a sufficiently large value of the required angular frequency cut-off $\omega_c$ of the electro-magnetic fluctuations, i.e. $\omega_c \gg \omega_A$, one obtains either a ${\cal O} (1/\tau)$ or a ${\cal O} (1/\tau^2)$ dependence. In the presence of physical boundaries, which can change the decay rate with many orders of magnitude, the conclusions remains the same after a suitable rescaling of parameters.Comment: 13 pages, 5 figures and 46 reference

Thermal spin flips in atom chips

Published versio

Macroscopic Interference Effects in Resonant Cavities

We investigate the possibility of interference effects induced by macroscopic quantum-mechanical superpositions of almost othogonal coherent states - a Schroedinger cats state - in a resonant microcavity. Despite the fact that a single atom, used as a probe of the cat state, on the average only change the mean number of photons by one unit, we show that this single atom can change the system drastically. Interference between the initial and almost orthogonal macroscopic quantum states of the radiation field can now take place. Dissipation under current experimental conditions is taken into account and it is found that this does not necessarily change the intereference effects dramatically.Comment: 20 pages, 3 figure

On the Theory of Casimir-Polder Forces

We consider the energy shift for an atom close to a non-magnetic body with a magnetic moment coupled to a quantized magnetic field. The corresponding repulsive Casimir-Polder force is obtained for a perfect conductor, a metal, a dielectric medium, with dielectric properties modeled by a Drude formula, and a superconductor at zero temperature. The dielectric properties of the superconductor is obtained by making use of the Mattis-Bardeen linear response theory and we present some useful expressions for the low-frequency conductivity. The quantum dynamics with a given initial state is discussed in terms of the well-known Weisskopf-Wigner theory and is compared with corresponding results for a electric dipole coupling. The results obtained are compatible with a conventional master equation approach. In order to illustrate the dependence on geometry and material properties, numerical results are presented for the ground state using a two-level approximation.Comment: 13 pages, 10 figures. Minor corrections. Published versio

Consensus guidelines for the definition, detection and interpretation of immunogenic cell death.

Cells succumbing to stress via regulated cell death (RCD) can initiate an adaptive immune response associated with immunological memory, provided they display sufficient antigenicity and adjuvanticity. Moreover, multiple intracellular and microenvironmental features determine the propensity of RCD to drive adaptive immunity. Here, we provide an updated operational definition of immunogenic cell death (ICD), discuss the key factors that dictate the ability of dying cells to drive an adaptive immune response, summarize experimental assays that are currently available for the assessment of ICD in vitro and in vivo, and formulate guidelines for their interpretation

Small lytic peptides escape the inhibitory effect of heparan sulfate on the surface of cancer cells

Several naturally occurring cationic antimicrobial peptides (CAPs), including bovine lactoferricin (LfcinB), display promising anticancer activities. These peptides are unaffected by multidrug resistance mechanisms and have been shown to induce a protective immune response against solid tumors, thus making them interesting candidates for developing novel lead structures for anticancer treatment. Recently, we showed that the anticancer activity by LfcinB was inhibited by the presence of heparan sulfate (HS) on the surface of tumor cells. Based on extensive structure-activity relationship studies performed on LfcinB, shorter and more potent peptides have been constructed. In the present study, we have investigated the anticancer activity of three chemically modified 9-mer peptides and the influence of HS and chondroitin sulfate (CS) on their cytotoxic activity. Various cell lines and red blood cells were used to investigate the anticancer activity and selectivity of the peptides. The cytotoxic effect of the peptides against the different cell lines was measured by use of a colorimetric MTT viability assay. The influence of HS and CS on their cytotoxic activity was evaluated by using HS/CS expressing and HS/CS deficient cell lines. The ability of soluble HS and CS to inhibit the cytotoxic activity of the peptides and the peptides’ affinity for HS and CS were also investigated. The 9-mer peptides displayed selective anticancer activity. Cells expressing HS/CS were equally or more susceptible to the peptides than cells not expressing HS/CS. The peptides displayed a higher affinity for HS compared to CS, and exogenously added HS inhibited the cytotoxic effect of the peptides. In contrast to the previously reported inhibitory effect of HS on LfcinB, the present study shows that the cytotoxic activity of small lytic peptides was increased or not affected by cell surface HS

The anticancer activity of lytic peptides is inhibited by heparan sulfate on the surface of the tumor cells

<p>Abstract</p> <p>Background</p> <p>Cationic antimicrobial peptides (CAPs) with antitumor activity constitute a promising group of novel anticancer agents. These peptides induce lysis of cancer cells through interactions with the plasma membrane. It is not known which cancer cell membrane components influence their susceptibility to CAPs. We have previously shown that CAPs interact with the two glycosaminoglycans (GAGs), heparan sulfate (HS) and chondroitin sulfate (CS), which are present on the surface of most cells. The purpose of this study was to investigate the role of the two GAGs in the cytotoxic activity of CAPs.</p> <p>Methods</p> <p>Various cell lines, expressing different levels of cell surface GAGs, were exposed to bovine lactoferricin (LfcinB) and the designer peptide, KW5. The cytotoxic effect of the peptides was investigated by use of the colorimetric MTT viability assay. The cytotoxic effect on wild type CHO cells, expressing normal amounts of GAGs on the cell surface, and the mutant pgsA-745, that has no expression of GAGs on the cell surface, was also investigated.</p> <p>Results</p> <p>We show that cells not expressing HS were more susceptible to CAPs than cells expressing HS at the cell surface. Further, exogenously added heparin inhibited the cytotoxic effect of the peptides. Chondroitin sulfate had no effect on the cytotoxic activity of KW5 and only minor effects on LfcinB cytotoxicity.</p> <p>Conclusion</p> <p>Our results show for the first time that negatively charged molecules at the surface of cancer cells inhibit the cytotoxic activity of CAPs. Our results indicate that HS at the surface of cancer cells sequesters CAPs away from the phospholipid bilayer and thereby impede their ability to induce cytolysis.</p