RELATIONSHIP AMONG ENTERO-TOXIGENIC PHENOTYPES, SEROTYPES, AND SOURCES OF STRAINS IN ENTERO-TOXIGENIC ESCHERICHIA-COLI

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Sistema informatizado para gerenciamento de indicadores da assistência de enfermagem do Hospital São Paulo

Os indicadores são instrumentos que possibilitam definir parâmetros que serão utilizados para realizar comparações e agregar juízo de valor frente ao encontrado e ao ideal estabelecido. Este estudo tem como objetivo descrever a experiência de um grupo de enfermeiras na criação do sistema informatizado desenvolvido no Hospital São Paulo para o gerenciamento de indicadores da assistência de enfermagem. A implantação deste sistema para o gerenciamento dos indicadores foi dividida em quatro etapas: desenvolvimento do manual de indicadores da assistência de enfermagem; realização de teste piloto registrado manualmente; desenvolvimento do sistema informatizado; implementação de estudo piloto do sistema informatizado em onze unidades do hospital

Production, characterization, and application of antibodies against heat-labile type-I toxin for detection of enterotoxigenic Escherichia coli

Strains of enterotoxigenic Escherichia coli (ETEC) are responsible for significant rates of morbidity and mortality among children, particularly in developing countries. The majority of clinical and public health laboratories are capable of isolating and identifying Salmonella, Shigella, Campylobacter, and Escherichia coli O157:H7 from stool samples, but ETEC cannot be identified by routine methods. The method most often used to identify ETEC is polymerase chain reaction for heat-stable and heat-labile enterotoxin genes, and subsequent serotyping, but most clinical and public health laboratories do not have the capacity or resources to perform these tests. In this study, polyclonal rabbit and monoclonal mouse IgG2b antibodies against ETEC heat-labile toxin-I (LT) were characterized and the potential applicability of a capture assay was analyzed. IgG-enriched fractions from rabbit polyclonal and the IgG2b monoclonal antibodies recognized LT in a conformational shape and they were excellent tools for detection of LT-producing strains. These findings indicate that the capture immunoassay could be used as a diagnostic assay of ETEC LT-producing strains in routine diagnosis and in epidemiological studies of diarrhea in developing countries as enzyme linked immunosorbent assay techniques remain as effective and economical choice for the detection of specific pathogen antigens in cultures