Reproducao em cativeiro do camarao rosa P. Paulensis (Perez Farfante, 1967) : estudo comparativo de diferentes...

Trabalho de Conclusão de Curso - Universidade Federal de Santa Catarina. Curso de Agronomia

Natural latex (Hevea brasiliensis) mold for neovaginoplasty

OBJETIVO: avaliar a utilização do molde de látex natural (Hevea brasiliensis) como modificação à neovaginoplastia de McIndoe e Bannister em pacientes portadoras da síndrome de Mayer-Rokitansky-Küster-Hauser (MRKH). MÉTODOS: análise retrospectiva de nove pacientes com o diagnóstico de síndrome de MRKH, submetidas à neovaginoplastia pela técnica de McIndoe e Bannister com molde de látex natural. Foram avaliadas epitelização, amplitude e profundidade das neovaginas, ocorrência de coitos bem como satisfação e complicações cirúrgicas. RESULTADOS: após cinco semanas do procedimento, oito pacientes apresentavam neovaginas epitelizadas, com profundidade de 7 a 12 cm. Houve um caso de estenose neovaginal completa em decorrência do uso incorreto do molde pela paciente no pós-operatório. Após seguimento mínimo de um ano, todas as pacientes mantinham neovaginas com profundidade de 4 a 8 cm e capacidade para o coito, com 66,7% de satisfação. Uma paciente apresentou fístula retovaginal precoce e episódios tardios de fistulização uretrovaginal. Duas pacientes apresentaram estenose distal das neovaginas a longo prazo. Uma delas e a paciente com fístulas foram submetidas a novo procedimento. CONCLUSÕES: o uso do molde de látex natural como modificação à técnica clássica de neovaginoplastia permitiu a criação de neovaginas morfológica e funcionalmente similares ao órgão normal em pacientes com estenose vaginal.PURPOSE: to evaluate the use of natural latex mold (Hevea brasiliensis) as a modification of McIndoe and Bannister neovaginoplasty in patients presenting Mayer-Rokitansky-Küster-Hauser (MKRH) syndrome. METHODS: we retrospectively included nine patients presenting MKRH syndrome, who had been submitted to McIndoe and Bannister neovaginoplasty modified by the use of natural latex mold. Neovaginal epithelization and depth, coitus occurrence and satisfaction, and surgical complications were evaluated. RESULTS: five weeks after the procedure, eight patients presented an epithelized 7 to 12 cm deep neovagina. There was one case of complete neovaginal stenosis, because of incorrect use of the mold. After at least one year, the others maintained 4 to 8 cm deep neovaginas and capacity for intercourse, with 66.7% satisfaction. One woman presented precocious rectovaginal fistula and late episodes of uretrovaginal fistulae. Two patients presented distal neovaginal stenosis in long-term follow-up. One of these and the patient with fistulae were submitted to a new procedure. CONCLUSIONS: the use of natural latex mold as a modification of classic neovaginoplasty technique allows the creation of neovaginas morphologically and functionally similar to the normal vagina in patients with vaginal agenesis

Fontes processuais historiográficas da Justiça do Trabalho: importância e vulnerabilidade

O presente artigo objetiva problematizar a importância e a vulnerabilidade das fontes processuais da Justiça do Trabalho. Inicialmente, pretende-se evidenciar as tentativas de extinção da legislação e do próprio Judiciário trabalhista, que, nos últimos tempos, tem sido amplamente agredido pela aplicação de políticas neoliberais. Em seguida, busca-se analisar as potencialidades e principais características dos documentos da Justiça do Trabalho como fonte historiográfica. O artigo se conclui com a apresentação de pesquisas recentemente realizadas no acervo Tribunal Regional do Trabalho da 12ª Região, com foco sobre os resultados obtidos pelos trabalhadores e trabalhadoras no ajuizamento de suas causas

Quantificação de depósitos e deriva de pulverização utilizando diferentes pontas em pulverizador costal no cafeeiro

The efficiency of foliar application of certain products on the coffee depends on the majority of cases, the coverage of the leaf surface with the product applied in spray form. This study aimed to quantify the deposit of liquid applied on coffee leaves and stems occurred when applied using a backpack sprayer, equipped with conical nozzles with different spray nozzle. The experimental design was the randomized block design (DBC) in a split-plot for the evaluation of drift, with five treatments (JD-10 known as “flat iron”; J4-2; J5-2; J6-2 and JA-2) with four replicates in both designs. To quantitatively evaluate the spray coverage the drift, was prepared a solution composed of the tracer brilliant blue food coloring coloring (FD & C Nº. 1) 0,15% (p /v) and sprayed over the coffee tree. To assess the amount of drift was used Petri dishes, which were distributed in the following points: the first plaque in the center of the plant the second plaque in the projection of the coffee tree and the third plate of 0.40 m projection of the coffee bar. The results showed that the tips JD-10, J4-2 and JA-2 were the ones that showed the best results providing lower losses during spraying.A eficiência da aplicação foliar de determinados produtos sobre o cafeeiro, depende, na maioria das vezes, da cobertura da superfície foliar com o produto aplicado em forma de pulverização. Neste estudo, objetivou-se quantificar o depósito do líquido aplicado sobre as folhas do cafeeiro e a deriva ocorrida, quando aplicado através de um pulverizador costal, munido de bicos cônicos com diferentes pontas de pulverização. O delineamento utilizado para ambos os experimentos foi o de blocos ao acaso (DBC), com cinco tratamentos: (JD-10 “chapinha” - UTC, J4-2, J5-2, J6-2 e JA-2), com quatro repetições e parcelas úteis com 6 plantas, sendo que, para a quantificação da deriva, utilizou-se esquema de parcelas subdivididas no espaço. A pulverização foi direcionada ao topo da planta do café em fase de formação (1 ano), localizada no município de Monte Santo de Minas/MG. Para avaliar quantitativamente os depósitos foi utilizada uma solução composta pelo corante alimentício azul brilhante (FD&C nº 1) a 0,15% (p/v). Em seguida, para verificar os depósitos de pulverização em folhas, coletaram-se 5 folhas do terço médio do cafeeiro para formar uma parcela e, para quantificação de deriva da pulverização foram colocadas placas de Petri distribuídas ao longo da parcela pulverizada nos seguintes pontos: a primeira placa no centro da planta, a segunda placa na projeção da barra do cafeeiro e a terceira placa a 0,40 m da projeção da barra do cafeeiro. Os resultados obtidos permitem concluir que a ponta J4-2 proporcionou maior acúmulo de depósitos de pulverização, em folhas de cafeeiro e menores perdas por deriva de pulverização, para as condições deste trabalho

Prevalence and characteristics of polycystic ovary syndrome in brazilian women : protocol for a nation-wide case-control study

Introduction Brazil is a large country, with a population of mixed ethnic background and broad variation in dietary and physical activity traits across its five main regions. Because data on Brazilian women with polycystic ovary syndrome (PCOS) are still scarce, a nation-wide collaborative study was designed to determine the prevalence of metabolic and reproductive abnormalities and the presence of anxiety and depression in Brazilian women with PCOS. In addition, the study aims at describing how these characteristics are distributed across PCOS phenotypes and at detecting associations with regional demographic and lifestyle aspects, genetic variants, and epigenetic markers. Methods and analysis The Brazilian PCOS study is being conducted in the outpatient clinics of eight university hospitals within the public healthcare network (Unified Health System) across the country. Additional centres will be included following completion of the research ethics approval process. The sample includes women with PCOS according to Rotterdam criteria at inclusion in the study and a control group of healthy women matched by age, socioeconomic status and geographical region. Data will be collected in each centre and incorporated into a unified cloud database. Clinical, demographic, socioeconomic, psychological, metabolic, epigenetic and genotypic variables will be evaluated. The data resulting from this study will be useful to guide specific public strategies for primary and secondary prevention of metabolic and reproductive comorbidities in the PCOS population of Brazil

Cardiovascular risk markers in polycystic ovary syndrome in women with and without insulin resistance

OBJETIVO: avaliar se a presença de resistência à insulina (RI) modifica fatores de risco cardiovascular em mulheres com síndrome dos ovários policísticos (SOP). MÉTODOS: estudo transversal no qual 60 mulheres com SOP, com idade entre 18 e 35 anos e sem uso de hormônios, foram avaliadas. A RI foi avaliada por meio do quantitative insulin sensitivity check index (QUICKI). RI foi definida como QUICKI <0,33. As seguintes variáveis foram comparadas entre o grupo com e sem RI: antropométricas (peso, altura, circunferência da cintura, pressão arterial e frequência cardíaca), laboratoriais (homocisteína, interleucina-6, fator de necrose tumoral-&#945;, testosterona, fração de androgênios livre, colesterol total e frações, triglicerídeos, proteína C reativa e insulina, glicose) e ultrassonográficas (distensibilidade e espessura íntima-média da carótida e dilatação mediada por fluxo da artéria braquial). RESULTADOS: Dezoito mulheres (30%) apresentaram RI. As mulheres com RI, comparadas às sem RI, apresentaram diferenças significativas nos seguintes marcadores antropométricos (SOP com RI e sem RI respectivamente): índice de massa corporal (35,5±5,6 versus 23,9±4,8 kg/m², p<0,01;), cintura (108,1±11,53 versus 79,5±11,1 cm, p<0,01) e pressão arterial sistólica (128,0±10,8 versus 114,0±8,9 mmHg, p<0,01) e pressão arterial diastólica (83,6±9,6 versus 77,0±7,5 mmHg, p=0,01). Também foram observadas diferenças significativas nos seguintes marcadores laboratoriais: triglicerídeos (120,0±56,5 versus 77,7±53,4 mg/dL, p=0,01), HDL (43,06±6,3 versus 40,4±10,8, p=0,01) e proteína C reativa (7,9±10,5 mg/L versus 2,6±3,2 mg/L, p<0,01), insulina (28,0±18,1 versus 5,3±2,4 µU/mL, p<0,01) e glicose (93,5±10,0 versus 87,5±8,7 mg/dL, p=0,02). Adicionalmente, dois dos três marcadores ultrassonográficos de risco cardiovascular também foram diferentes entre os grupos: distensibilidade carotídea (0,24±0,05 versus 0,30±0,08 mmHg-1, p<0,01) e espessura íntima-média da carótida (0,52±0,08 versus 0,43±0,09 mm, p<0,01). Além disso, a proporção de síndrome metabólica foi maior nas mulheres com RI (nove casos=50% versus três casos=7,1%, p<0,01). CONCLUSÕES: mulheres com SOP e RI apresentam diferenças significativas em vários marcadores ultrassonográficos, séricos e antropométricos que apontam para uma elevação no risco cardiovascular, quando comparadas a mulheres com SOP sem RI. Diante desses dados, a determinação sistemática da avaliação de RI em mulheres com SOP pode ajudar a identificar pacientes de risco cardiovascular.PURPOSE: to evaluate whether the presence of insulin resistance (IR) alters cardiovascular risk factors in women with polycystic ovary syndrome (POS). METHODS: transversal study where 60 POS women with ages from 18 to 35 years old, with no hormone intake, were evaluated. IR was assessed through the quantitative insulin sensitivity check index (QUICKI) and defined as QUICKI <0.33. The following variables have been compared between the groups with or without IR: anthropometric (weight, height, waist circumference, arterial blood pressure, cardiac frequency), laboratorial (homocysteine, interleucines-6, factor of tumoral-&#945; necrosis, testosterone, fraction of free androgen, total cholesterol and fractions, triglycerides, C reactive protein, insulin, glucose), and ultrasonographical (distensibility and carotid intima-media thickness, dilation mediated by the brachial artery flux). RESULTS: Eighteen women (30%) presented IR and showed significant differences in the following anthropometric markers, as compared to the women without IR (POS with and without IR respectively): body mass index (35.56±5.69 kg/m² versus 23.90±4.88 kg/m², p<0.01), waist (108.17±11.53 versus 79.54±11.12 cm, p<0.01), systolic blood pressure (128.00±10.80 mmHg versus 114.07±8.97 mmHg, p<0.01), diastolic blood pressure (83.67±9.63 mmHg versus 77.07±7.59 mmHg, p=0.01). It has also been observed significant differences in the following laboratorial markers: triglycerides (120.00±56.53 mg/dL versus 77.79±53.46 mg/dL, p=0.01), HDL (43.06±6.30 mg/dL versus 40.45±10.82 mg/dL, p=0.01), reactive C protein (7.98±10.54 mg/L versus 2.61±3.21 mg/L, p<0.01), insulin (28.01±18.18 µU/mL versus 5.38±2.48 µU/mL, p<0.01), glucose (93.56±10.00 mg/dL versus 87.52±8.75 mg/dL, p=0.02). Additionally, two out of the three ultrasonographical markers of cardiovascular risk were also different between the groups: carotid distensibility (0.24±0.05 mmHg-1 versus 0.30±0.08 mmHg-1, p<0.01) and carotid intima-media thickness (0.52±0.08 mm versus 0.43±0.09, p<0.01). Besides, the metabolic syndrome ratio was higher in women with IR (nine cases=50% versus three cases=7.1%, p<0.01). CONCLUSIONS: POS and IR women present significant differences in several ultrasonographical, seric and anthropometric markers, which point out to higher cardiovascular risk, as compared to women without POS and IR. In face of that, the systematic IR evaluation in POS women may help to identify patients with cardiovascular risk

Obesity and altered arterial structure in young women with micropolycystic ovary syndrome

OBJETIVO: comparar os fatores ecográficos de risco cardiovascular em pacientes obesas e não obesas, com síndrome dos ovários micropolicísticos (SOMP). MÉTODOS: foram incluídas 30 pacientes obesas com SOMP (Índice de massa corporal, IMC>30 kg/m²) e 60 não obesas (IMC<30 kg/m²), com idade entre 18 e 35 anos neste estudo transversal. Foram avaliados: a dilatação mediada por fluxo (DMF) da artéria braquial, espessura íntima-média da artéria carótida (IMT), o índice de rigidez da artéria carótida (&#946;), as medidas antropométricas, pressão sanguínea sistólica (PAS) e diastólica (PAD). As mulheres estavam sem nenhum tratamento prévio e nenhuma delas apresentava qualquer comorbidade (além da SOMP e/ou da obesidade).Na análise estatística, foram utilizados os testes t não-pareado ou de Mann-Whitney. RESULTADOS: as pacientes obesas com SOMP apresentaram maior peso em relação às não obesas (92,1±11,7 kg versus 61,4±10,7 kg, p<0,0001), bem como a medida da cintura que também, foi mais elevada nas pacientes obesas (105,0±10,4 cm versus 78,5±9,8 cm, p<0,0001). A PAS das pacientes obesas foi superior quando comparadas às não obesas (126,1±10,9 mmHg versus 115,8±9,0 mmHg, p<0,0001) e a IMT também foi maior nas obesas (0,51±0,07 mm versus 0,44±0,09 mm, p<0,0001). Não houve diferença entre os grupos quanto à dilatação mediada por fluxo (DMF) da artéria braquial ou ao índice de rigidez da artéria carótida (&#946;). CONCLUSÕES: a obesidade em portadoras jovens de SOMP está associada a níveis pressóricos mais elevados e à alteração da estrutura arterial, representada pela maior espessura íntima-média da artéria carótida.PURPOSE: to compare echographical cardiovascular risk factors between obese and non-obese patients with micropolycystic ovarian syndrome (MPOS). METHODS: in this transversal study, 30 obese (Body Mass Index, BMI>30 kg/m²) and 60 non-obese (BMI<30 kg/m²) MPOS patients, aging between 18 and 35 years old, were included. The following variables were measured: flow-mediated dilatation (FMD) of the brachial artery, thickness of the intima-media of the carotid artery (IMT), anthropometric data, systolic arterial pressure (SAP) and diastolic arterial pressure (DAP). The women had no previous medical treatment and no comorbidity besides MPOS and obesity. For statistical analysis, the non-paired tand Mann-Whitney's tests were used. RESULTS: obese weighted more than non-obese patients (92.1±11.7 kg versus 61.4±10.7 kg, p<0.0001) and had a larger waist circumference (105.0±10.4 cm versus 78.5±9.8 cm, p<0.0001). The SBP of obese patients was higher than that of the non-obese ones (126.1±10.9 mmHg versus 115.8±9.0 mmHg, p<0.0001) and the IMT was also bigger (0.51±0.07 mm versus 0.44±0.09 mm, p<0.0001). There was no significant difference between the groups as to FMD and carotid rigidity index (&#946;). CONCLUSIONS: obesity in young women with MPOS is associated with higher blood pressure and alteration of arterial structure, represented by a thicker intima-media of the carotid artery

Raloxifene therapy does not affect uterine blood flow in postmenopausal women: a transvaginal Doppler study

AZEVEDO, George Dantas de et al. Raloxifene therapy does not affect uterine blood flow in postmenopausal women: a transvaginal Doppler study. Maturitas, Amsterdam, v.47, n.3, p.195-200, 2004Objective:To monitor the effects of raloxifene therapy on the uterus of postmenopausalwomen by transvaginal ultrasonography and color flow Doppler. Methods: Twenty-five healthy postmenopausal women were enrolled in this prospective longitudinal study performed at Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto. The patients were treated with raloxifene hydrochloride (60 mg per day) for 6 months. All were submitted to transvaginal ultrasound examination with color flow Doppler (ATL-HDI 3000 equipment) before the beginning and after 1, 3 and 6 months of treatment. Resistance index (RI) and pulsatility index (PI) of the uterine arteries were determined by the Doppler method, being considered as indicators of uterine perfusion. The following variables were analyzed: endometrial thickness, uterine volume, RI, and PI. Data were analyzed statistically by repeated-measures analysis of variance. Results: Before treatment, endometrial thickness was 3.38 ± 0.73 mm, and similar values were observed after 1, 3 and 6 months of treatment (3.04 ± 0.82; 3.3 ± 0.83; and 3.37 ± 0.79, respectively) (P > 0.05). No significant differences in uterine volume were observed between the pre- and post-treatment periods. Uterine artery perfusion as indicated by RI and PI measured by Doppler also showed no significant variation, with a high impedance flow being maintained throughout treatment. Conclusions: In the group studied here, raloxifene treatment at the dose of 60 mg per day for 6 months did not induce significant changes in endometrial thickness, uterine volume or uterine artery perfusion, confirming that short-term raloxifene treatment does not affect the uterus of postmenopausal wome

Immune expression of E-cadherin and alpha, beta and gamma-Catenin adhesion molecules and prognosis for upper urinary tract urothelial carcinomas

Introduction: Cell adhesion molecules (CAM) are required for maintaining a normal epithelial phenotype, and abnormalities in CAM expression have been related to cancer progression, including bladder urothelial carcinomas. There is only one study that correlates E-cadherin and alpha-, beta- and gamma-catenin expression with prognosis of upper tract urothelial carcinomas. Our aim is to study the pattern of immune expression of these CAMs in urothelial carcinomas from the renal pelvis and ureter in patients who have been treated surgically. Our goal is to correlate these expression levels and characteristics with well-known prognostic parameters for disease-free survival. Materials and Methods: We evaluated specimens from 20 patients with urothelial carcinomas of the renal pelvis and ureter who were treated with nephroureterectomy or ureterectomy between June 1997 and January 2007. CAM expression was evaluated by immunohistochemistry in a tissue microarray and correlated with histopathological characteristics and patient outcomes after a mean follow-up of 55 months. Results: We observed a relationship between E-cadherin expression and disease recurrence. Disease recurrence occurred in 87.5% of patients with strong E-cadherin expression. Only 50.0% of patients with moderate expression and 0% of patients with weak or no expression of E-cadherin had disease recurrence (p = 0.014). There was also a difference in disease-free survival. Patients with strong E-cadherin expression had a mean disease-free survival rate of 49.1 months, compared to 83.9 months for patients with moderate expression (p = 0.011). Additionally, an absence of a-catenin expression was associated with tumors that were larger than 3 cm (p = 0.003). Conclusions: We demonstrated for the first time that immune expression of E-cadherin is related to tumor recurrence and disease-free survival rates, and the absence of a-catenin expression is related to tumor size in upper tract urothelial carcinomas