Técnicas interactivas en el desarrollo de la expresión oral de los niños y niñas de 4 a 5 años.

El el lenguaje es básico en la interrelación general de las personas, en el aprendizaje en la relación social, en la comunicación y por otro lado un área básica dentro del currículo escolar a lo largo de todo proceso educativo. En aquellos casos que este se altera o se produce un retraso en su adquisición por causas diversas es necesario ejecutar programas de intervención educativa. En aquellos casos que este se altera o produce un retraso un retraso en su adquisición por causas diversas es necesario ejecutar programas de intervención educativa. Es fundamental su desarrollo porque permite mejorar los procesos cognitivos, comunicativos y sociales. Cuando este no ese ha dado por diversos motivos es necesario buscar las estrategias adecuadas para el desarrollo del mismo. Tanto en el hogar como en la escuela, ofrece una gama de oportunidades para hacerlo pero cuando los padres o docentes desconocen esto, generan en el niño o niña vacíos en el lenguaje. La selección de técnicas interactivas como la que proponemos en este proyecto la cual responden a necesidades concretas como por ejemplo potenciar determinados aspectos del vocabulario, el uso de reglas gramaticales, acceder a una luida comunicación, etc. Cabe recordar que el niño o niña o ser humano entiende el doble de lo que expresa porque siempre hay una duplicidad en esta función. Por ende es necesario que se busque alternativas que permitan su mejoramiento, y enriquecimiento, labor que debe ser ejecutada por la docente y reforzada en el hogar por los padres de familia

Optimization of Oil and Tocopherol Extraction from Maqui (Aristotelia chilensis (Mol.) Stuntz) by Supercritical CO2 Procedure

This study focused on the oil extraction from freeze-dried maqui (Aristotelia chilensis) by supercritical fluid extraction with carbon dioxide (SFE-CO2). The basic objective was to optimize the oil yield and the tocopherol concentration. A Box/Behnken experimental design was developed with three processing variables: supercritical pressure (74, 187, and 300 bar), temperature (35, 48, and 60 °C), and extracting time (30, 135, and 240 min). Multiple optimizations, based on the combination of factor levels at 274 bar, 240 min, and 60 °C, led to the highest oil yield and tocopherol values. The validation of the optimized conditions of maqui oil extraction led to an oil yield of 8% and values of 735, 53, and 97 (mg·kg−1 oil) for α-tocopherol, α-tocotrienol, and γ-tocopherol, respectively. A higher concentration of tocopherol compounds was observed when compared to the employment of the conventional extracting method. The optimized SFE-CO2 method led to an oil extract exhibiting higher Hydrophilic-Oxygen Radical Absorbance Capacity (H-ORAC) assay and total phenol content (22 μmol Trolox equivalents·g−1 oil and 28 mg gallic acid equivalents·g−1 oil) than the oil obtained by the conventional procedure. A practical and accurate oil extraction is proposed for obtaining tocopherol-enriched oil including high concentrations of valuable lipophilic antioxidants

Do Clinical Trials Meet Current Care Needs? Views of Digestive Oncology Specialists in Galicia (Spain) Using the Delphi Method

Background: In recent years, abundant scientific evidence has been generated based on clinical trials (CT) in the field of oncology. The general objective of this paper is to find out the extent to which decision making is based on knowledge of the most recent CT. Its specific objectives are to pinpoint difficulties with decision making based on the CT performed and find out the motivations patients and clinicians have when taking part in a CT. Methodology: Combined, prospective study, based on the Delphi method. A lack of correspondence between the people who take part in CT and patients who come for consultation has been identified. A need for training in analysing and interpreting CT has also been identified and a lack of trust in the results of CT financed by the pharmaceutical industry itself has been perceived. Conclusions: There is a difficulty in selecting oncological treatment due to the lack of correspondence between the patients included in the CT and patients seen in consultation. In this process, real world data studies may be highly useful, as they may provide this group with greater training in interpreting CT and their results.S

Monitoring of kinetics and exhaustion markers of circulating CAR-T cells as early predictive factors in patients with B-cell malignancies

© 2023 García-Calderón, Sierro-Martínez, García-Guerrero, Sanoja-Flores, Muñoz-García, Ruiz-Maldonado, Jimenez-Leon, Delgado-Serrano, Molinos-Quintana, Guijarro-Albaladejo, Carrasco-Brocal, Lucena, García-Lozano, Blázquez-Goñi, Reguera-Ortega, González-Escribano, Reinoso-Segura, Briones, Pérez-Simón and Caballero-Velázquez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Purpose: CAR-T cell therapy has proven to be a disruptive treatment in the hematology field, however, less than 50% of patients maintain long-term response and early predictors of outcome are still inconsistently defined. Here, we aimed to optimize the detection of CD19 CAR-T cells in blood and to identify phenotypic features as early biomarkers associated with toxicity and outcomes.Experimental design: In this study, monitoring by flow cytometry and digital PCR (dPCR), and immunophenotypic characterization of circulating CAR-T cells from 48 patients treated with Tisa-cel or Axi-cel was performed.Results: Validation of the flow cytometry reagent for the detection of CAR-T cells in blood revealed CD19 protein conjugated with streptavidin as the optimal detection method. Kinetics of CAR-T cell expansion in blood confirmed median day of peak expansion at seven days post-infusion by both flow cytometry and digital PCR. Circulating CAR-T cells showed an activated, proliferative, and exhausted phenotype at the time of peak expansion. Patients with increased expansion showed more severe CRS and ICANs. Immunophenotypic characterization of CAR-T cells at the peak expansion identified the increased expression of co-inhibitory molecules PD1 and LAG3 and reduced levels of the cytotoxicity marker CD107a as predictors of a better long-term disease control.Conclusions: These data show the importance of CAR-T cells in vivo monitoring and identify the expression of PD1LAG3 and CD107a as early biomarkers of long-term disease control after CAR-T cell therapy.This work has been supported by the Instituto de Salud Carlos III (ISCIII), Project RD21/0017/0021, Red Española de Terapias Avanzadas TERAV funded by European Union-NextGenerationEU. “Plan de Recuperación Transformación y Resiliencia” and Consejería de Salud y Familia, Junta de Andalucía PECART-0185-2020-7, PECART-0185-2020 CSYF 2021 – Proyectos Fondos FEDER. Proyectos estratégicos en Investigación en CAR-T. “Monitorización inmune tras tratamiento con células CAR-T: búsqueda de biomarcadores y medición de la actividadmetabólica como predictores de respuesta”.Peer reviewe

Trabajo fin de máster

Treball final de Màster Universitari en Psicologia del Treball, de les Organitzacions i en Recursos Humans. Codi: SBE013. Curs acadèmic 2015-2016En este documento se desarrolla el trabajo fin del Máster Universitario en Psicología del Trabajo, de las Organizaciones y en Recursos Humanos de la Universidad Jaume I. De manera inicial, se realizará una exposición de los temas más relevantes que han contribuido en mi formación teórica a lo largo de todas las asignaturas desarrollas en el curso lectivo. A continuación, se realizará una valoración de las competencias adquiridas en el máster una vez finalizadas las prácticas profesionales. Todo ello, con el objetivo, de tratar de hacer una recopilación de las competencias adquiridas antes y después de la realización del máster, teniendo en cuenta la formación teórica y la estancia en empresa. Posteriormente, se desarrollará una valoración personal destacando los aspectos positivos de la formación e indicado algunas medidas de mejora que el alumnado, creemos, puedan corregirse. Y, para finalizar, una explicación del futuro profesional que nos gustaría tener intentando relacionarlo con los contenidos estudiados en el máster.In this document it is to be developed, a final master’s degree report in the psychology of work, organisations and human resources of the university Jaume I. For the start, there will be a presentation of the issues that I think have been more important in my theoretical training throughout all the subjects that I developed during the school year. It will be followed by an assessment of the skills acquired in the master once the professional practices are completed. This will be done with the addition of recording the acquired skills before and after the masters, taking into account the teaching training and business's practices. Subsequently, a personal assessment highlighting the positive aspects of academic training will be developed. With the indication of some measures of improvements that the students we believe, can be corrected. To finish, an explanation of the professional future that is expected, in accordance with what we have studied in the masters

Segundas víctimas: calidad de soporte percibido y su relación con las consecuencias del evento adverso

Introduction: The second victims are those health workers who are involved in an Adverse Event (AE) and display physical, emotional, psychological and / or work-related symptoms. To avoid these conditions, it is important to receive supportive measures. The objective of this article is to determine the relationship between the consequences of an AE on the second victims and the quality of the support measures received in public and private health institutions of the Metropolitan Region during the second semester of 2018. Materials and Method: Quantitative, exploratory, descriptive, correlational and transversal study. Through an online platform, a sociodemographic survey and SVEST instrument was conducted, consisting of 28 items on the Likert scale (1-5) and nine dimensions related to the consequences of the AE and the quality of the perceived support (α = 0.826). Results: Of a sample of 301 health workers in public and private institution, 39.2% were invol¬ved in an EA and of these, 73.0% manifested symptoms of Second Victims. Of these second victims, 69.1% were female and 45.7% worked in the Nursing Profession. There is a negative relationship between the quality of perceived support and the psychological and occupational consequence of AD (Public and Private pIntroducción: Las segundas víctimas son aquellos trabajadores de salud que están involucrados en un Evento Adverso (EA) y presentan afección física, emocional, psicológica y/o laboral. Para evitar estas afecciones, es importante recibir medidas de soporte. El objetivo de este artículo es determinar qué relación existe entre las consecuencias de un EA sobre las segundas víctimas y la calidad de las medidas de soporte percibidas en instituciones de salud públicas y privadas de la Región Metropolitana durante el segundo semestre del año 2018. Materiales y método: Estudio cuantitativo, exploratorio, descriptivo, correlacional y transversal. A través de una plataforma online se aplicó una encuesta sociodemográfica e instrumento SVEST que consta de nueve dimensiones relacionadas con consecuencias del EA y calidad del soporte percibido (α=0,826). Resultados: Muestra de 301 trabajadores de salud entre institución pública y privada, el 39,2% se involucró en un EA y de estos, el 73,0% manifestó ser Segunda Víctima. De estas segundas víctimas, 69,1% pertenece al sexo femenino y el 45,7% se desempeña como Profesional de Enfermería. Existe una relación negativa entre calidad de soporte percibido y consecuencia psicológica y laboral del EA (Público y Privado

Anomia y Alienación en Estudiantes Secundarios de Santiago de Chile: Resultados Iniciales de un Estudio Comparativo 1989-2007 Anomie and Alienation in High School Students in Santiago of Chile: Initial Results of a Comparative Research Study 1989-2007

Se presentan los avances de un estudio sobre percepción de integración social y normativa en estudiantes de cuarto año de educación media de Santiago (N = 343). Se analizan los resultados de un cuestionario sobre anomia y alienación psicosocial, validado en un estudio análogo realizado en 1989. La replicación en 2007 contribuye a la caracterización de la experiencia de integración social y normativa en los jóvenes de hoy, así como al análisis de sus transformaciones casi 20 años después. Los resultados muestran que no existe una clara diferencia en los niveles de anomia y alienación, respecto a los de 1989.<br>In a sample of Chilean students in their last year of high school (N = 343) anomie and psychosocial alienation were measured with a questionnaire validated in an analogous study done in 1989. This study compared the results of 1989 with those of 2007. This comparison contributes to the psychosocial characterization of social and normative integration experiences of Chilean youth today, and to the analysis of their transformations approximately 20 years later. The results show that there is not a clear difference in the social and normative integration levels in the studied youth, with respect to those of 1989

The Behçet's disease-associated variant of the aminopeptidase ERAP1 shapes a low-affinity HLA-B*51 peptidome by differential subpeptidome processing.

A low-activity variant of endoplasmic reticulum aminopeptidase 1 (ERAP1), Hap10, is associated with the autoinflammatory disorder Behçet's disease (BD) in epistasis with HLA-B*51, which is the main risk factor for this disorder. The role of Hap10 in BD pathogenesis is unknown. We sought to define the effects of Hap10 on the HLA-B*51 peptidome and to distinguish these effects from those due to HLA-B*51 polymorphisms unrelated to disease. The peptidome of the BD-associated HLA-B*51:08 subtype expressed in a Hap10-positive cell line was isolated, characterized by mass spectrometry, and compared with the HLA-B*51:01 peptidome from cells expressing more active ERAP1 allotypes. We additionally performed synthetic peptide digestions with recombinant ERAP1 variants and estimated peptide-binding affinity with standard algorithms. In the BD-associated ERAP1 context of B*51:08, longer peptides were generated; of the two major HLA-B*51 subpeptidomes with Pro-2 and Ala-2, the former one was significantly reduced, and the latter was increased and showed more ERAP1-susceptible N-terminal residues. These effects were readily explained by the low activity of Hap10 and the differential susceptibility of X-Pro and X-Ala bonds to ERAP1 trimming and together resulted in a significantly altered peptidome with lower affinity. The differences due to ERAP1 were clearly distinguished from those due to HLA-B*51 subtype polymorphism, which affected residue frequencies at internal positions of the peptide ligands. The alterations in the nature and affinity of HLA-B*51·peptide complexes probably affect T-cell and natural killer cell recognition, providing a sound basis for the joint association of ERAP1 and HLA-B*51 with BD

Enzymatic synthesis of EPA- and DHA-enriched structured acylglycerols at the sn-2 position starting from commercial salmon oil and concentrated by response surface methodology under supercritical conditions

Poster.-- 50th Conference of the Western European Fish Technologists Association, Rotterdam, Netherlands, 17th - 21st October 2022Marine oils are widely used due to their high content of omega 3 long-chain polyunsaturated fatty acids (n-3 LCPUFA), such as eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) since its consumption is associated with a low prevalence of heart, circulatory and inflammatory diseases. The bio-availability of such fatty acids has been shown to depend on their location in triacylglycerol (TG) mulecules at the sn-2 position. Consequently, great attention has been accorded to the synthesis of structured acylglycerols (SAcyl), which includes EPA or DHA at the sn-2 position. Supercritical fluids, such as carbon dioxide (C01), have solvent power. lts density can be changed and manipulated as a function of pressure and temperature since, at its critical pressure, its compressibility is maximum and small changes in thermal parameters can lead to significant changes in local density. Different types of biocatalysts, such as lipases, can be used to modify lipids. They are the most versatile since their regiospecificity is one of the main advantages for modifying oils and fats to obtain high added-value products, such as EPA + UHA supplements. This work aimed to optimize the synthesis of a SAcyl from commercial refined salmon oil (CRSO) and a concentrate of n-3 LCPUFA obtained from the same oil to enhance the positioning of EPA and DHA in sn-2 in glycerol backbone by enzymatic acidolysls in supercritical C02 (C02SC)ANID/CONICYT + FONDECYT Regular + folio (1181774 y 1221633).N