Heparin modulates the expression of genes encoding pro and anti-apoptotic proteins in endothelial cells exposed to intestinal ischemia and reperfusion in rats

PURPOSE: To investigate if expression of genes encoding pro and anti-apoptotic proteins in the rat enteric endothelial cells stimulated by intestinal ischemia followed by reperfusion (IR) can be modified by treatment with heparin (HP).METHODS: Eighteen adult Wistar rats were divided in three groups: sham group submitted to laparotomy only (SG), ischemia followed by reperfusion group (IRG); ischemia followed by reperfusion plus pretreatment with HP 100 mg.kg-1 (IRG+HP). Ischemia was performed by clamping of the superior mesenteric artery. After 60 min of ischemia, metal clamps were removed for reperfusion for 120 min. Gene expression of encoding pro (Casp1, Casp6, Casp3, Cflar, Fas and Pgl) and anti-apoptotic (Bcl2, Bcl2l1 and Naip2) proteins in rat enteric endothelial cells was evaluated by PCR microarray method.RESULTS: Compared to rat endothelial cells of SG, the expression of pro-apoptotic genes was up-regulated in IRG while anti-apoptotic genes were down-regulated. In contrast, the expression of anti-apoptotic genes in IRG+HP was up-regulated while pro-apoptotic genes was down-regulated compared to SG.CONCLUSION: The attenuation by heparin of intestinal ischemia-reperfusion previously demonstrated in rodents could be related with ability of this drug to stimulate and reduce gene expression of encoding anti and pro-apoptotic proteins, respectively.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Federal University of São Paulo Department of SurgeryUNIFESP Pharmacology DepartmentUNIFESP Gynecology DepartmentUniversidade Federal de São Paulo (UNIFESP) Department of BiochemistryUniversidade Federal de São Paulo (UNIFESP) Department of SurgeryUNIFESP, Department of SurgeryUNIFESP, Pharmacology DepartmentUNIFESP, Gynecology DepartmentUNIFESP, Department of BiochemistryUNIFESP, Department of SurgerySciEL

Ischemic preconditioning and the gene expression of enteric endothelial cell biology of rats submitted to intestinal ischemia and reperfusion

PURPOSE: To investigate the effects of ischemic preconditioning (IPC) on the expression of pro and anti-apoptotic genes in rat endothelial cells undergoing enteric ischemia (I) and reperfusion (R). METHODS: Thirty rats underwent clamping of the superior mesenteric vessels. Sham group (GS) laparotomy only; Ischemia (GI): intestinal ischemia (60 min); Ischemia and Reperfusion (GIR): ischemia (60 min) and reperfusion (120 min); Ischemia and intestinal ischemic preconditioning (GI + IPC) : 5 minutes of ischemia followed by 10 min of reperfusion before sustained ischemia (60 min) ischemia and reperfusion and IPC (GIR + IPC): 5 min ischemia followed by 10 min of reperfusion before sustained ischemia (60min) and reperfusion (120 min). Rat Endothelial Cell Biology (PCR array) to determine the expression of genes related to endothelial cell biology. RESULTS: Gene expression of pro-apoptotic markers (Casp1, Casp6, Cflar, Fas, and Pgl) was down regulated in GI+IPC and in GIR + IPC. In contrast, the expression of anti-apoptotic genes (Bcl2 and Naip2), was up-regulated in GI + IPC and in GIR + IPC. CONCLUSION: Ischemic preconditioning may protect against cell death caused by ischemia and reperfusion.UNIFESPUNIFESPSciEL

A pílula mágica da gripezinha: a narrativa da cloroquina nos grupos bolsonaristas de WhatsApp durante a pandemia de Covid-19

Este trabalho analisa o modo como se construiu o universo narrativo da cloroquina como cura/tratamento para a Covid-19 nos grupos de WhatsApp bolsonaristas. Para tal, exploram-se duas linhas argumentativas com base na possibilidade de haver a produção e manutenção de uma teoria conspiratória focada na lógica dicotômica do bem contra o mal. O primeiro argumento destaca a maneira como a narrativa dos benevolentes se estrutura em defesa de uma crença autossustentada sobre a eficácia do produto, que, de acordo com Cassam (2019), caracteriza um "vício epistêmico". O segundo refere-se à construção da narrativa por meio de uma rede de posições ideologicamente orientadas para criar antagonistas, que interpretamos à luz da noção de "baixa polícia", a partir de Rancière (1996, 2014). Como estratégia metodológica de análise, foi elaborada uma cronologia entre as principais mensagens sobre a cloroquina difundidas em grupos bolsonaristas, acessadas por meio do Monitor de Whatsapp, desenvolvido pela UFMG. A relação cronológica das mensagens permitiu apreender as dominâncias de cada fase da construção narrativa, de modo a reuni-las em uma rede discursiva, cuja análise tenta responder à questão investigada. Palavras- chave: Universo narrativo da cloroquina. WhatsApp bolsonaristas. Semiótica

Grupo de Pesquisa em Enfermagem na Prevenção e Controle de Infecções: 20 anos de contribuições

Relato de experiência sobre as atividades realizadas por um Núcleo de Estudos e Pesquisa em Enfermagem, no desenvolvimento da temática de prevenção e controle de infecções relacionadas à assistência à saúde. O estudo teve como objetivo descrever a trajetória histórica, a importância e contribuição de um grupo de pesquisa da área de prevenção e controle de infecção em seus vinte anos de existência. O texto discute a trajetória do grupo, que foi pioneiro na área deenfermagem na região Centro Oeste do Brasil e destaca a relevância da experiência no fortalecimento, consolidação da produção de conhecimento dos pesquisadores envolvidos e na formação de jovens pesquisadores. O grupo de pesquisa constitui-se em oportunidade de compartilhar conhecimentos teóricos e práticos, instigar estudantes e docentes à criação e aos estudos, com foco na busca de soluções para problemas identificados na realidade

Seasonality effect on ant (Hymenoptera: Formicidae) activity in an ecotonal environment in the state of Piauí, Brazil

This study aimed to compare the activity of ants during the rainy and dry seasons in a transitional environment (ecotone) at the interface of Cerrado and Caatinga biomes situated in the state of Piauí, where studies of local ant fauna are still incipient. The research was carried out at Floriano, state of Piauí, Brazil. Ants were sampled during the rainy season in December 2018 and during the dry season in June 2019. Three parallel transects were established at least 100 m apart. At each point, three sampling methods were used, with attractive baits placed on the trees and ground, and pitfall traps on the ground. Thirty-one ant species belonging to 14 genera and five subfamilies were collected, with 27 recorded in the rainy season and 17 in the dry season. The only species reported with the three collection methods and the two seasons was Camponotus crassus Mayr, 1862. Seven ant species sampled in this study are new reports for the state of Piauí. There was a significant difference in the average number of ants and the composition of the assemblages between the rainy and dry seasons. We demonstrate that the activity of ants is influenced by seasonality, corroborating our hypotheses. This is only the second study on ant diversity in the state of Piauí, and more studies are necessary to understand the processes and factors which regulate the activity patterns of ants in ecotonal situations like those observed in this region

Study of Heparin in Intestinal Ischemia and Reperfusion in Rats: Morphologic and Functional Evaluation

To study whether treatment with heparin (HEP) attenuates intestinal dysfunction caused by ischemia (I) and reperfusion (R), rats were treated with HEP (100 U/kg intravenously) or saline solution (SS) before I (60 min), which was produced by occlusion of the superior mesenteric artery, and R (120 min). After I or I/R, we mounted 2-cm jejunal segment in an organ bath to study neurogenic contractions stimulated by electrical pulses or KCl, using a digital recording system. Thin jejunal slices were stained with hematoxylin and eosin for optical microscopy. Compared with the sham group, jejunal contractions were similar in the I + HEP and the I/R + HEP groups, but reduced in the I + SS and the I/R + SS groups. the jejunal enteric nerves were damaged in the I + SS and the I/R + SS, but not in the I + HEP and the I/R + HEP cohorts. These results suggested that HEP attenuated intestinal dysfunction caused by I and I/R.Universidade Federal de São Paulo, Escola Paulista Med, Dept Surg, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pharmacol, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biochem, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morphol, BR-04023900 São Paulo, BrazilFed Univ Great Dourados, Sch Med, Dourados, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Surg, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pharmacol, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biochem, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morphol, BR-04023900 São Paulo, BrazilWeb of Scienc