3,081 research outputs found
Recommended from our members
Longitudinal Monitoring of SARS-CoV-2 IgM and IgG Seropositivity to Detect COVID-19.
BackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a novel beta-coronavirus that has recently emerged as the cause of the 2019 coronavirus pandemic (COVID-19). Polymerase chain reaction (PCR) based tests are optimal and recommended for the diagnosis of an acute SARS-CoV-2 infection. Serology tests for viral antibodies provide an important tool to diagnose previous exposure to the virus. Here we evaluate the analytical performance parameters of the Diazyme SARS-CoV-2 IgM/IgG serology assays and describe the kinetics of IgM and IgG seroconversion observed in patients with PCR-confirmed COVID-19 who were admitted to our hospital.MethodsWe validated the performance of the Diazyme assay in 235 presumed SARS-CoV-2 negative subjects to determine specificity. Subsequently, we evaluated the SARS-CoV-2 IgM and IgG seroconversion of 54 PCR-confirmed COVID-19 patients and determined sensitivity of the assay at three different timeframes.ResultSensitivity and specificity for detecting seropositivity at ≥15 days following a positive SARS-CoV-2 PCR result, was 100.0% and 98.7% when assaying for the panel of IgM and IgG. The median time to seropositivity observed for a reactive IgM and IgG result from the date of a positive PCR was 5 days (IQR: 2.75-9 days) and 4 days (IQR: 2.75-6.75 days), respectively.ConclusionsOur data demonstrate that the Diazyme IgM/IgG assays are suited for the purpose of detecting SARS-CoV-2 IgG and IgM in patients with suspected SARS-CoV-2 infections. For the first time, we report longitudinal data showing the evolution of seroconversion for both IgG and IgM in a cohort of acutely ill patients in the United States. We also demonstrate a low false positive rate in patients who were presumed to be disease free
On the master equation approach to kinetic theory: linear and nonlinear Fokker--Planck equations
We discuss the relationship between kinetic equations of the Fokker-Planck
type (two linear and one non-linear) and the Kolmogorov (a.k.a. master)
equations of certain N-body diffusion processes, in the context of Kac's
"propagation of chaos" limit. The linear Fokker-Planck equations are
well-known, but here they are derived as a limit N->infty of a simple linear
diffusion equation on (3N-C)-dimensional N-velocity spheres of radius sqrt(N)
(with C=1 or 4 depending on whether the system conserves energy only or energy
and momentum). In this case, a spectral gap separating the zero eigenvalue from
the positive spectrum of the Laplacian remains as N->infty,so that the
exponential approach to equilibrium of the master evolution is passed on to the
limiting Fokker-Planck evolution in R^3. The non-linear Fokker-Planck equation
is known as Landau's equation in the plasma physics literature. Its N-particle
master equation, originally introduced (in the 1950s) by Balescu and Prigogine
(BP), is studied here on the (3N-4)-dimensional N-velocity sphere. It is shown
that the BP master equation represents a superposition of diffusion processes
on certain two-dimensional sub-manifolds of R^{3N} determined by the
conservation laws for two-particle collisions. The initial value problem for
the BP master equation is proved to be well-posed and its solutions are shown
to decay exponentially fast to equilibrium. However, the first non-zero
eigenvalue of the BP operator is shown to vanish in the limit N->infty. This
indicates that the exponentially fast approach to equilibrium may not be passed
from the finite-N master equation on to Landau's nonlinear kinetic equation.Comment: 20 pages; based on talk at the 18th ICTT Conference. Some typos and a
few minor technical fixes. Modified title slightl
Recommended from our members
Allantoin Crystal Formation in Bagrada hilaris (Burmeister) (Heteroptera: Pentatomidae) Females.
Bagrada hilaris is a polyphagous herbivore reported as an invasive pest in the United States. During the course of dissecting Burmeister hilaris unique crystals were observed in both the midgut and oviducts. Crystals were identified using X-ray diffraction techniques. Both acicular (i.e., needle-like, slender, and/or tapered) and cubic (i.e., cube shaped) crystals were observed in six of 75 individuals examined (8.0%). The crystals were mainly observed in females (6.7%), followed by males (1.3%) with no crystals observed in the minimal number of nymphs examined (0%). Crystals of both types were detected in the midgut and lateral oviducts of the females and midgut in males. The acicular crystals often appeared as distinct bundles when present in the midgut and oviducts. Crystals varied in size with the acicular crystals ranging from 0.12 mm to 0.5 mm in length although the cubic crystals ranged in length from 0.25 mm to over 1.0 mm with widths of ∼0.25 mm. The cubic crystals were identified as allantoin although the acicular crystals were most likely dl-allantoin in combination with halite. While allantoin in a soluble form is often found in insect tissues and excreta; being present as a crystal, especially in such a large form, is curious and raises some interesting questions. More research is warranted to further understand mechanisms associated with such crystal formation in B. hilaris and can lead to a better understanding of the excretory process in this species and the role allantoin plays in the elimination of excess nitrogen
Wave operator bounds for 1-dimensional Schr\"odinger operators with singular potentials and applications
Boundedness of wave operators for Schr\"odinger operators in one space
dimension for a class of singular potentials, admitting finitely many Dirac
delta distributions, is proved. Applications are presented to, for example,
dispersive estimates and commutator bounds.Comment: 16 pages, 0 figure
Elevated corticosterone in feathers correlates with corticosterone-induced decreased feather quality: A validation study
The newly described technique of extracting corticosterone (CORT) from bird feathers may serve as a less invasive, more integrated measure of a bird\u27s stress response. Previous work indicated that elevated plasma CORT resulted in poorer quality feathers during molt. We tested the hypothesis that a direct link exists between plasma and feather CORT concentrations. We experimentally increased plasma CORT concentrations using implants and found that the corresponding rise in CORT could be detected in feathers grown during implantation. Furthermore, CORT levels in two feathers grown at the same time from the same bird were very consistent. These results provide evidence that elevated CORT is a causative factor in decreasing feather quality during molt. However, there remain technical details that suggest caution when interpreting data from CORT extracted from feathers. Different portions of a growing feather did not necessarily reflect changes in plasma CORT at the time different parts of the feather were forming, a standard pool of homogenized feathers indicated that sample mass affects measured feather CORT concentration, and different antibodies produced different measured CORT concentrations, leaving in doubt the exact steroid being assayed. © 2011 The Authors
Are receptor concentrations correlated across tissues within individuals? A case study examining glucocorticoid and mineralocorticoid receptor binding
Copyright © 2015 by the Endocrine Society. Hormone receptors are a necessary (although not sufficient) part of the process through which hormones like corticosterone create physiological responses. However, it is currently unknown to what extent receptor concentrations across different target tissues may be correlated within individual animals. In this study, we examined this question using a large dataset of radioligand binding data for glucocorticoid receptors (GRs) and mineralocorticoid receptors (MRs) in 13 different tissues in the house sparrow (Passer domesticus) (n = 72). Our data revealed that individual house sparrows tended to exhibit higher or lower receptor binding across all tissues, which could be part of what creates the physiological and behavioral syndromes associated with different hormonal profiles. However, although statistically significant, the correlations between tissues were very weak. Thus, when each tissue was independently regressed on receptor concentrations in the other tissues, multivariate analysis revealed significant relationships only for sc fat (for GR) and whole brain, hippocampus, kidney, omental fat, and sc fat (for MR). We also found significant pairwise correlations only between receptor concentrations in brain and hippocampus, and brain and kidney (both for MR). This research reveals that although there are generalized individual consistencies in GR and MR concentrations, possibly due to such factors as hormonal regulation and genetic effects, the ability of 2 different tissues to respond to the same hormonal signal appears to be affected by additional factors that remain to be identified
Calibrations of phase abundance, composition, and particle size distribution for olivine-orthopyroxene mixtures from reflectance spectra
Spectral reflectance measurements of characterized (phase abundance, particle size) mixtures of olivine and orthopyroxene were utilized to define the correlations between spectral and albedo parameters of such assemblages and their mineralogical or textural properties. Thirty-three different spectral parameters falling into three general classes (relative or ratioed, absolute or albedo, and wavelength) were investigated for empirical sensitivity to one or more of the mixture properties. Theoretical considerations and previous experimental observations were utilized to understand their functional relationships. The ratio of areas for the 1- and 2-3µm absorption bands is shown to be a sensitive indicator of the olivine-orthopyroxene abundance and is very nearly independent of particle size and mineral composition. In conjunction with an abundance determination, the wavelength position of the 1-3tm absorption feature can be utilized to determine the molar iron contents of the olivine and orthopyroxene phases. This calibration is insensitive to particle size but will produce systematic deviations if the phases have significantly different iron contents or if more than a few percent of a clinopyroxene component is present. The spectral albedo in the 0.6- to 0.7-µm region is relatively insensitive to phase abundance and can be used to constrain particle size if phase composition has been determinedVarious portions of this work were supported at the University of Hawaii by NASA grant NSG-7312 and by a Sigma Xi Foundation grant to E.A.C. and at Rensselaer Polytechnic Institute by NASA grant NAGW-642. Planetary Geosciences Division, Hawaii Institute of Geophysics, University of Hawaii, publication4 70
Population III star formation in a Lambda CDM universe, I: The effect of formation redshift and environment on protostellar accretion rate
(abridged) We perform 12 extremely high resolution adaptive mesh refinement
cosmological hydrodynamic simulations of Population III star formation in a
Lambda CDM universe, varying the box size and large-scale structure, to
understand systematic effects in the formation of primordial protostellar
cores. We find results that are qualitatively similar to those observed
previously. We observe that the threshold halo mass for formation of a
Population III protostar does not evolve significantly with time in the
redshift range studied (33 > z > 19) but exhibits substantial scatter due to
different halo assembly histories: Halos which assembled more slowly develop
cooling cores at lower mass than those that assemble more rapidly, in agreement
with Yoshida et al. (2003). We do, however, observe significant evolution in
the accretion rates of Population III protostars with redshift, with objects
that form later having higher maximum accretion rates, with a variation of two
orders of magnitude (10^-4 - 10^-2 Msolar/year). This can be explained by
considering the evolving virial properties of the halos with redshift and the
physics of molecular hydrogen formation at low densities. Our result implies
that the mass distribution of Population III stars inferred from their
accretion rates may be broader than previously thought, and may evolve with
redshift. Finally, we observe that our collapsing protostellar cloud cores do
not fragment, consistent with previous results, which suggests that Population
III stars which form in halos of mass 10^5 - 10^6 Msun always form in
isolation.Comment: Accepted by The Astrophysical Journal. Some minor changes. 65 pages,
3 tables, 21 figures (3 color). To appear in January 1, 2007 issu
Decay of Quantum Accelerator Modes
Experimentally observable Quantum Accelerator Modes are used as a test case
for the study of some general aspects of quantum decay from classical stable
islands immersed in a chaotic sea. The modes are shown to correspond to
metastable states, analogous to the Wannier-Stark resonances. Different regimes
of tunneling, marked by different quantitative dependence of the lifetimes on
1/hbar, are identified, depending on the resolution of KAM substructures that
is achieved on the scale of hbar. The theory of Resonance Assisted Tunneling
introduced by Brodier, Schlagheck, and Ullmo [9], is revisited, and found to
well describe decay whenever applicable.Comment: 16 pages, 11 encapsulated postscript figures (figures with a better
resolution are available upon request to the authors); added reference for
section
The Hartree limit of Born's ensemble for the ground state of a bosonic atom or ion
The non-relativistic bosonic ground state is studied for quantum N-body
systems with Coulomb interactions, modeling atoms or ions made of N "bosonic
point electrons" bound to an atomic point nucleus of Z "electron" charges,
treated in Born--Oppenheimer approximation. It is shown that the (negative)
ground state energy E(Z,N) yields the monotonically growing function (E(l N,N)
over N cubed). By adapting an argument of Hogreve, it is shown that its limit
as N to infinity for l > l* is governed by Hartree theory, with the rescaled
bosonic ground state wave function factoring into an infinite product of
identical one-body wave functions determined by the Hartree equation. The proof
resembles the construction of the thermodynamic mean-field limit of the
classical ensembles with thermodynamically unstable interactions, except that
here the ensemble is Born's, with the absolute square of the ground state wave
function as ensemble probability density function, with the Fisher information
functional in the variational principle for Born's ensemble playing the role of
the negative of the Gibbs entropy functional in the free-energy variational
principle for the classical petit-canonical configurational ensemble.Comment: Corrected version. Accepted for publication in Journal of
Mathematical Physic
- …