Формування та оцінка податкового потенціалу регіонів України

У статті розглянуті теоретичні підходи до розкриття сутності поняття «податковий потенціал», досліджено питання оцінки податкового потенціалу, а також запропоновано науково-методичні підходи щодо визначення впливу податкового потенціалу на соціально-економічний розвиток регіонів України. The article deals with theoretical approaches to elucidate the essence of the concept of “tax a potential”, theoretical approaches to determination of tax potential and suggested the scientific and methodical approaches to determined the impact of tax potential to the social and economic regional’s development

Automation of optimization synthesis for modular technological equipment

Technological equipment design based on functionally modular methods is widely used in various technical fields. The designed object can be a technological machine, a production line, or a manufacturing complex. Special attention is paid to the optimization of its structure. The sequence of performing all stages of the optimization synthesis problem is presented in the article. To find a solution to this task, the developer should apply the complete or directed search of acceptable structure options and determine the best one using some optimization criteria to evaluate their quality. It can be simple enough if the designed technical system structure consists of no more than several elements. For example, if the number of alternative elements options is several dozen, it takes much time to accomplish the search correctly. Thus, the greater the number of components considered, the more difficult it is to do all the necessary calculations manually. In this case, machine resources should be involved. This scientific work aims to identify procedures of optimization synthesis that can be automated. Also, appropriate software has to be developed. Our computer program is based on the algorithm of a complete search of all options of the technical system structure. It can process an extensive array of input data and produce all possible and logically permissible results in the form the designer can analyze using the Pareto method to choose the best one. This software can be used for any technical system with a modular structure.info:eu-repo/semantics/publishedVersio

Radial-piston pump for drive of test machines

The article reviews the development of radial-piston pump with phase control and alternating-flow mode for seismic-testing platforms and other test machines. The prospects for use of the developed device are proved. It is noted that the method of frequency modulation with the detection of the natural frequencies is easily realized by using the radial-piston pump. The prospects of further research are given proof

Teriparatide and Pelvic Fracture Healing: A Phase 2 Randomized Controlled Trial

Purpose: To determine if teriparatide (20 ug/day; TPTD) results in improved radiologic healing, reduced pain and improved functional outcome vs. placebo over 3 months in pelvic fracture patients. Methods: This randomized-placebo-controlled study enrolled 35 patients (women and men ≥50 years old) within 4 weeks of pelvic fracture and evaluated the effect of blinded TPTD versus placebo over 3 months on fracture healing. Fracture healing from CT images at 0 and 3 months was assessed as cortical bridging using a 5-point scale. The numeric rating scale (NRS) for pain was administered monthly. Physical performance was assessed monthly by Continuous Summary Physical Performance Score (based on 4m walk speed, timed repeated chair stands, and balance) and the Timed Up and Go (TUG) test. Results: The mean age was 82 and >80% were female. The intention to treat analysis showed no group difference in cortical bridging score and 50% of fractures in TPTD-treated and 53% of fractures in placebo-treated patients were healed at 3 months, unchanged after adjustment for age, sacral fracture, and fracture displacement. Median pain score dropped significantly in both groups with no group differences. Both CSPPS and TUG improved in the teriparatide group, whereas there was no improvement in the placebo group (group difference p<0.03 for CSPPS at 2 and 3 months). Conclusion: In this small randomized, blinded study, there was no improvement in radiographic healing (CT at 3 months) or pain with TPTD vs placebo, however, there was improved physical performance in TPTD-treated subjects that was not evident in the placebo group

Получение компактной меди из карбонатной руды электрохимическим способом

Розглянуто процес електроекстракції міді з розчинів, отриманих вилуговуванням міді з карбонатної руди сульфатною кислотою. Показано, що обробку руди доцільно проводити 10 %-ною кислотою впродовж 30-40 хв при інтенсивному перемішуванні суміші. Здійснено процес електроекстракції міді з концентрованого розчину при ступеневому зниженні густини струму пропорційно до концентрації іонів міді, що зменшується в ході процесу. Виконано порівняння параметрів реального процесу і розрахованого за теоретично отриманими формулами. Встановлено, що прогнозування оптимального режиму електролізу можливо здійснювати за електрохімічними характеристиками, початковою концентрацією металу і геометричними параметрами електрохімічної комірки. Одержано експериментальну залежність напруги на електролізері від концентрації кислоти, іонів міді та густини катодного струму. Виявлено, що основною складовою напруги на електролізері є електрохімічна — різниця потенціалів електродів під струмом. Показано зменшення напруги в часі й розраховано питомі витрати електроенергії при отриманні міді методом електроекстракції.A process of compact copper electrowinning from the solution obtained by leaching copper from the ore by sulfuric acid was discussed. It was shown that the ore leaching is advisable to carry out with 10 % hydrochloric acid for 30-40 minutes with intensive stirring of the mixture. Copper electrowinning process was done from a concentrated solution with a stepped reduction of the current density which was proportional to the copper ion concentration decreasing during the process. The comparison of the parameters of the real and theoretical calculated processes was performed. It was found that the prediction of optimal electrolysis conditions can be achieved by electrochemical characteristics, initial metal concentration and geometric parameters of the electrochemical cell. An experimental cell voltage dependence of the acid concentration, copper ions and the cathode current density was determined. It was found that the main component of the cell voltage is electrochemical component — the difference potential at the electric current. The decreasing of the cell voltage in time was shown and the relative cost of electricity in the producing of copper by electrowinning was calculated.Рассмотрен процесс электроэкстракции меди из растворов, полученных выщелачиванием меди из карбонатной руды серной кислотой. Показано, что обработку руды целесообразно проводить 10 %-ной кислотой в течение 30-40 мин при интенсивном перемешивании смеси. Осуществлен процесс электроэкстракции меди из концентрированного раствора при ступенчатом снижении плотности тока пропорционально концентрации ионов меди, уменьшающейся в ходе процесса. Выполнено сравнение параметров реального процесса и теоретически рассчитанного по формулам. Установлено, что прогнозирование оптимального режима электролиза возможно осуществлять с использованием электрохимических характеристик, начальной концентрации металла и геометрических параметров электрохимической ячейки. Получена экспериментальна зависимость напряжения на электролизере от концентрации кислоты, ионов меди и плотности катодного тока. Определено, что основной составляющей напряжения на электролизере является электрохимическая — разность потенциалов электродов под током. Показано уменьшение напряжения во времени, рассчитаны дельные затраты электроэнергии при получении меди методом электроэкстракции

Localization of Human RNase Z Isoforms: Dual Nuclear/Mitochondrial Targeting of the ELAC2 Gene Product by Alternative Translation Initiation

RNase Z is an endonuclease responsible for the removal of 3′ extensions from tRNA precursors, an essential step in tRNA biogenesis. Human cells contain a long form (RNase ZL) encoded by ELAC2, and a short form (RNase ZS; ELAC1). We studied their subcellular localization by expression of proteins fused to green fluorescent protein. RNase ZS was found in the cytosol, whereas RNase ZL localized to the nucleus and mitochondria. We show that alternative translation initiation is responsible for the dual targeting of RNase ZL. Due to the unfavorable context of the first AUG of ELAC2, translation apparently also starts from the second AUG, whereby the mitochondrial targeting sequence is lost and the protein is instead routed to the nucleus. Our data suggest that RNase ZL is the enzyme involved in both, nuclear and mitochondrial tRNA 3′ end maturation

Carrier transport in porous-SiNic-Si nanostructures

In the present paper we have studied the peculiarities of carrier transport properties of nanoheterostructures containing silicon substrate covered with porous silicon layer, where pores were either filled or non-filled with ferromagnetic Ni clusters.We have carried out DC conductivity experiments as a function of temperature (ranging from 2 to 300 K) and porosity of porous silicon layer (between 30% and 70%). Presence of a surface layer with high resistance on the porous silicon top and its role in nanoheterostructure formation was revealed. It was shown that specific electrochemical kinetics of Ni deposition into porous silicon significantly influences resultant nanostructure resistance and high temperature conductance activation energy

Разработка методики получения дермального внеклеточного матрикса

Despite advancements in modern surgery in the treatment of cutaneous injuries, the search for new methods of ensuring faster and more effective wound healing appears especially urgent today. Tissue engineering is undoubtedly of interest when it comes to developing such technologies. Objective: to determine the optimal protocol for obtaining a decellularized dermal matrix scaffold for subsequent development of tissue-engineered skin. Materials and methods. One Landrace piglet was used as the experimental animal. After preliminary skin treatment with dermatome, 0.3 cm thick samples were taken. Two decellularization protocols were considered: protocol No. 1 was based on the use of Triton X-100 and deoxycholate, protocol No. 2 was only based on deoxycholate. There were 5 processing cycles in total for the 2 protocols. After treatment, acellular matrix scaffolds were examined through histological analysis and quantitative determination of DNA concentration. Next, static recellarization of the matrix scaffolds was carried out with porcine dermal fibroblasts. After that, the matrix scaffolds were tested for cytotoxicity using XTT test and differential staining test to differentiate between live and dead cells. Results. Comparative analysis of the two protocols for porcine dermis decellularization showed that both protocols effectively remove cells and nuclear material, while maintaining the architectonics of the intercellular substance intact, since fibrous structures are not destroyed. But when assessing the biocompatibility of matrix scaffolds based on analysis of cell viability according to data obtained from XTT test and cell–matrix adhesion, the matrix scaffold processed under protocol No. 1, shows advantages. Conclusion. In this study, a decellularization protocol based on Triton X-100 and deoxycholate was noted. The results obtained mark the first stage towards developing a tissue-engineered skin.Несмотря на достижения современной хирургии в лечении повреждений кожных покровов, актуальным остается поиск новых методов для более быстрого и эффективного заживления ран. Тканевая инженерия, несомненно, представляет интерес для разработки таких технологий. Цель данной работы состояла в определении оптимального протокола получения децеллюляризированного дермального матрикса для последующей разработки тканеинженерной кожи. Материалы и методы. Экспериментальным животным был 1 поросенок породы Ландрас. После предварительной обработки кожи дерматомом забирали образцы толщиной 0,3 см. В работе рассматривалось 2 протокола децеллюляризации: протокол № 1 на основе применения тритон Х100 и дезоксихолата, протокол № 2 только на основе дезоксихолата. Всего циклов обработки по 2 протоколам было 5. Ацеллюлярные матриксы после обработки были исследованы следующим образом: гистологический анализ, количественное определение содержания ДНК. Далее была проведена статическая рецеллюляризация матриксов фибробластами дермы свиньи. После чего матриксы были исследованы на цитотоксичность с помощью ХТТ-теста и теста на дифференциальное окрашивание живых и погибших клеток. Результаты. Проведенный сравнительный анализ двух протоколов децеллюляризации дермы свиной кожи показал, что оба протокола эффективно удаляют клетки и ядерный материал, при этом сохраняется архитектоника межклеточного вещества неповрежденной, так как не происходит разрушения волокнистых структур. Но при оценке биосовместимости матриксов на основе анализа жизнеспособности клеток по данным ХТТ-теста и адгезии клеток к матриксу преимущества демонстрирует матрикс, обработанный по протоколу № 1. Заключение. В настоящем исследовании был отмечен протокол децеллюляризации на основе тритон Х100 и дезоксихолата. Полученные результаты являются первым этапом для дальнейшей разработки тканеинженерной кожи

A survey of green plant tRNA 3'-end processing enzyme tRNase Zs, homologs of the candidate prostate cancer susceptibility protein ELAC2

<p>Abstract</p> <p>Background</p> <p>tRNase Z removes the 3'-trailer sequences from precursor tRNAs, which is an essential step preceding the addition of the CCA sequence. tRNase Z exists in the short (tRNase Z^S) and long (tRNase Z^L) forms. Based on the sequence characteristics, they can be divided into two major types: bacterial-type tRNase Z^Sand eukaryotic-type tRNase Z^L, and one minor type, <it>Thermotoga maritima </it>(TM)-type tRNase Z^S. The number of tRNase Zs is highly variable, with the largest number being identified experimentally in the flowering plant <it>Arabidopsis thaliana</it>. It is unknown whether multiple tRNase Zs found in <it>A. thaliana </it>is common to the plant kingdom. Also unknown is the extent of sequence and structural conservation among tRNase Zs from the plant kingdom.</p> <p>Results</p> <p>We report the identification and analysis of candidate tRNase Zs in 27 fully sequenced genomes of green plants, the great majority of which are flowering plants. It appears that green plants contain multiple distinct tRNase Zs predicted to reside in different subcellular compartments. Furthermore, while the bacterial-type tRNase Z^Ss are present only in basal land plants and green algae, the TM-type tRNase Z^Ss are widespread in green plants. The protein sequences of the TM-type tRNase Z^Ss identified in green plants are similar to those of the bacterial-type tRNase Z^Ss but have distinct features, including the TM-type flexible arm, the variant catalytic HEAT and HST motifs, and a lack of the PxKxRN motif involved in CCA anti-determination (inhibition of tRNase Z activity by CCA), which prevents tRNase Z cleavage of mature tRNAs. Examination of flowering plant chloroplast tRNA genes reveals that many of these genes encode partial CCA sequences. Based on our results and previous studies, we predict that the plant TM-type tRNase Z^Ss may not recognize the CCA sequence as an anti-determinant.</p> <p>Conclusions</p> <p>Our findings substantially expand the current repertoire of the TM-type tRNase Z^Ss and hint at the possibility that these proteins may have been selected for their ability to process chloroplast pre-tRNAs with whole or partial CCA sequences. Our results also support the coevolution of tRNase Zs and tRNA 3'-trailer sequences in plants.</p