The improvement in regenerated doubled haploids from anther culture of wheat by anther transfer

This study was conducted to determine the most suitable method of regeneration by comparing two approaches: transfer of anthers (with and without embryo-like structures) to regeneration conditions after a period of two to four weeks on induction medium (= anther-transfer treatment) and transfer of embryo-like structures to regeneration conditions after five to eight weeks on induction medium. The early transfer of anthers brought about a significant reduction in the number of embryos formed, but nevertheless significantly improved the frequency of plant regeneration. Combining an optimal date of anther transfer with the early addition of colchicine to the induction medium (100 mg l−1 for 1 and 3 days) led to an increase in the number of doubled haploid regenerants. The results indicate that transferring the anthers after 28 days and adding 100 mg l−1 colchicine to the induction medium on one day only caused a significant improvement in the ability of green plants to regenerate (7.0 compared to 0.50) as well as in chromosome doubling (success index: 4.0 compared to 0.33

Production of cornelian cherry (Cornus mas L.) pulp powder by foam-mat drying: analysis of physicochemical and antioxidant properties

This is the final version, Available on open access from Springer via the DOI in this recordAvailability of data and materials: Data available within the article or its supplementary materialsCornelian cherry (Cornus mas L.) is a rich source of (poly)phenols such as phenolic acids, flavonoids, and anthocyanins that are known for their bioactivity. This study aimed to enhance the industrial applications of this cherry’s pulp as a potential functional food ingredient. Foam-mat drying was used to produce cherry powder from cherry pulp. The convective drying process was performed at different temperatures (50, 60, 70, and 80°C) to study the effect of drying temperature on the physical properties, flow properties, microstructure, (poly)phenol content, antioxidant properties, and bioaccessibility of foam-dried cherry pulp, in comparison to freeze-dried sample. Different drying temperatures did not affect color properties. The highest gloss was determined at high temperatures (70–80°C). According to the Carr index, the fluidity increased between 18 and 22% as the temperature increased. The total phenolic content was higher at elevated temperatures (70–80°C), yet the anthocyanin content was the highest at 60°C. The flavonoid content was not affected by different drying temperatures, as well as compared to lyophilized samples. The antioxidant activity was higher in samples dried at elevated temperatures, with no significant difference in comparison to lyophilized samples. The bioaccessibility experiment showed that the lyophilized samples had significantly greater bioaccessibility in comparison to the convective dried samples, yet further investigation is required to understand the differences in bioaccessibility and bioavailability of individual polyphenols. Overall, foam-mat drying using a convective drying system could be an economic choice to preserve acceptable amounts of phenolics and antioxidants

Compromised dental cells viability following teeth-whitening exposure

This study aimed to assess the viability of dental cells following time-dependent carbamide peroxide teeth-whitening treatments using an in-vitro dentin perfusion assay model. 30 teeth were exposed to 5% or 16% CP gel (4 h daily) for 2-weeks. The enamel organic content was measured with thermogravimetry. The time-dependent viability of human dental pulp stem cells (HDPSCs) and gingival fibroblast cells (HGFCs) following either indirect exposure to 3 commercially available concentrations of CP gel using an in-vitro dentin perfusion assay or direct exposure to 5% H2O2 were investigated by evaluating change in cell morphology and by hemocytometry. The 5% and 16% CP produced a significantly lower (p < 0.001) enamel protein content (by weight) when compared to the control. The organic content in enamel varied accordingly to the CP treatment: for the 16% and 5% CP treatment groups, a variation of 4.0% and 5.4%, respectively, was observed with no significant difference. The cell viability of HDPSCs decreased exponentially over time for all groups. Within the limitation of this in-vitro study, we conclude that even low concentrations of H2O2 and CP result in a deleterious change in enamel protein content and compromise the viability of HGFCs and HDPSCs. These effects should be observed in-vivo

Development of auditing in Malaysia: Legal, political and historical influences

This work investigates the role and contribution of external auditing as practised in the Malaysian society during the forty year period from independence in 1957 to just before the onset of the Asian Financial Crisis in 1997.It applies the political economic theory introduced by Tinker (1980) and refined by Cooper & Sherer (1984), which focuses on the social relations aspects of professional activity rather than economic forces alone.In a case study format where qualitative data was gathered mainly from primary and secondary source materials, the study found that the function of auditing in the Malaysian society in most cases is devoid of any essence of mission; instead it is created, shaped and transformed by the pressures which give rise to its development over time.The largely insignificant role that it serves is intertwined within the contexts in which it operates

Volume coil based on hybridized resonators for magnetic resonance imaging

International audienc

The improvement in regenerated doubled haploids from anther culture of wheat by anther transfer

ISSN:0167-6857ISSN:1573-504