Improved design of electrophoretic equipment for rapid sickle-cell-anemia screening

Effective mass screening may be accomplished by modifying existing electrophoretic equipment in conjunction with multisample applicator used with cellulose-acetate-matrix test paper. Using this method, approximately 20 to 25 samples can undergo electrophoresis in 5 to 6 minutes

New challenges for scholarly communication in the digital era - changing roles and expectations in the academic community: a scholarly report

Journal ArticleThis conference, co-sponsored by the Association of Research Libraries, the American Association of University Professors, the American Council of Learned Societies, the Association of American University Presses, and the Coalition for Networked Information, was held March 26-27, 1999, in Washington, D.C. It was organized, in part, as a follow-up to a similar conference held in September 1997, titled The Specialized Scholarly Monograph in Crisis

QUANTITATION AND IMMUNOCYTOCHEMICAL LOCALIZATION OF HUMAN SKIN COLLAGENASE IN BASAL CELL CARCINOMA

Human skin collagenase was quantitated by radioimmunoassay in 21 basal cell carcinomas. Immunoreactive collagenase protein was found to be approximately 2-fold greater in extracts of these tumors than in extracts of normal skin, suggesting that this enzyme may be important in the pathogenesis of soft tissue destruction in vivo. To further define the role of collagenase in such destruction, immunofluorescent staining with specific antiserum to human skin collagenase was used to localize collagenase in the basal cell carcinomas. The enzyme was found only in the stromal elements surrounding the tumor islands. No staining of the epithelial components of the basal cell carcinomas was found. These findings suggest that the normal connective tissue elements may have been stimulated to produce an increased amount of collagenase and emphasize the importance of epithelial-stromal interaction in soft tissue invasiveness

RedMaPPer: Evolution and Mass Dependence of the Conditional Luminosity Functions of Red Galaxies in Galaxy Clusters

We characterize the luminosity distribution, halo mass dependence, and redshift evolution of red galaxies in galaxy clusters using the SDSS Data Release 8 RedMaPPer cluster sample. We propose a simple prescription for the relationship between the luminosity of both central and satellite galaxies and the mass of their host halos, and show that this model is well-fit by the data. Using a larger galaxy cluster sample than previously employed in the literature, we find that the luminosities of central galaxies scale as $\langle \log L \rangle \propto A_L \log (M_{200b})$, with $A_L=0.39\pm0.04$, and that the scatter of the central--galaxy luminosity at fixed $M_{200b}$ ( $\sigma_{\log L|M}$) is $0.23 ^{+0.05}_{-0.04}$ dex, with the error bar including systematics due to miscentering of the cluster finder, photometry, and photometric redshift estimation. Our data prefers a positive correlation between the luminosity of central galaxies and the observed richness of clusters at a fixed halo mass, with an effective correlation coefficient $d_{\rm{eff}}=0.36^{+0.17}_{-0.16}$. The characteristic luminosity of satellites becomes dimmer from $z=0.3$ to $z=0.1$ by $\sim 20\%$ after accounting for passive evolution. We estimate the fraction of galaxy clusters where the brightest galaxy is not the central to be $P_{\rm{BNC}} \sim 20\%$. We discuss implications of these findings in the context of galaxy evolution and the galaxy--halo connection.Comment: 29 pages, 17 figures, 5 tables. Accepted by AP

Impact of Strain on Drain Current and Threshold Voltage of Nanoscale Double Gate Tunnel Field Effect Transistor: Theoretical Investigation and Analysis

Tunnel field effect transistor (TFET) devices are attractive as they show good scalability and have very low leakage current. However they suffer from low on-current and high threshold voltage. In order to employ the TFET for circuit applications, these problems need to be tackled. In this paper, a novel lateral strained double-gate TFET (SDGTFET) is presented. Using device simulation, we show that the SDGTFET has a higher on-current, low leakage, low threshold voltage, excellent subthreshold slope, and good short channel effects and also meets important ITRS guidelines.Comment: http://web.iitd.ac.in/~mamidal

PksS from Bacillus subtilis is a cytochrome P450 involved in bacillaene metabolism

As part of the pksX gene cluster of Bacillus subtilis strain 168, pksX has been preliminarily annotated as a cytochrome P450 homolog that hydroxylates the polyketide product of this cluster, which was recently shown to be involved in the biosynthesis of bacillaene and dihydrobacillaene. Here we report that there is a frame-shift error in the reported sequence for pksX, and that we have successfully cloned, overexpressed, and purified the protein encoded by the corrected sequence. By utilizing electronic absorption spectrophotometry, we have observed that the ferrous CO complex of pksX absorbs maximally near 450 nm, which confirms the annotation that this protein is a cytochrome P450. We have also established a cell-free system derived from crude cytosolic B. subtilis protein extracts which provides reductase activity essential to sustaining the putative catalytic cycle of pksX. Using LC–MS analysis we have collected data which suggests that the substrate for pksX is dihydrobacillaene

Mapping analysis and planning system for the John F. Kennedy Space Center

Environmental management, impact assessment, research and monitoring are multidisciplinary activities which are ideally suited to incorporate a multi-media approach to environmental problem solving. Geographic information systems (GIS), simulation models, neural networks and expert-system software are some of the advancing technologies being used for data management, query, analysis and display. At the 140,000 acre John F. Kennedy Space Center, the Advanced Software Technology group has been supporting development and implementation of a program that integrates these and other rapidly evolving hardware and software capabilities into a comprehensive Mapping, Analysis and Planning System (MAPS) based in a workstation/local are network environment. An expert-system shell is being developed to link the various databases to guide users through the numerous stages of a facility siting and environmental assessment. The expert-system shell approach is appealing for its ease of data access by management-level decision makers while maintaining the involvement of the data specialists. This, as well as increased efficiency and accuracy in data analysis and report preparation, can benefit any organization involved in natural resources management