239 research outputs found
Small Near-Earth Asteroids in the Palomar Transient Factory Survey: a Real-Time Streak-detection System
Near-Earth asteroids (NEAs) in the 1β100 meter size range are estimated to be ~1,000 times more numerous than the ~15,000 currently cataloged NEAs, most of which are in the 0.5β10 kilometer size range. Impacts from 10β100 meter size NEAs are not statistically life-threatening, but may cause significant regional damage, while 1β10 meter size NEAs with low velocities relative to Earth are compelling targets for space missions. We describe the implementation and initial results of a real-time NEA-discovery system specialized for the detection of small, high angular rate (visually streaked) NEAs in Palomar Transient Factory (PTF) images. PTF is a 1.2-m aperture, 7.3 deg^2 field of view (FOV) optical survey designed primarily for the discovery of extragalactic transients (e.g., supernovae) in 60-second exposures reaching ~20.5 visual magnitude. Our real-time NEA discovery pipeline uses a machine-learned classifier to filter a large number of false-positive streak detections, permitting a human scanner to efficiently and remotely identify real asteroid streaks during the night. Upon recognition of a streaked NEA detection (typically within an hour of the discovery exposure), the scanner triggers follow-up with the same telescope and posts the observations to the Minor Planet Center for worldwide confirmation. We describe our 11 initial confirmed discoveries, all small NEAs that passed 0.3β15 lunar distances from Earth. Lastly, we derive useful scaling laws for comparing streaked-NEA-detection capabilities of different surveys as a function of their hardware and survey-pattern characteristics. This work most directly informs estimates of the streak-detection capabilities of the Zwicky Transient Facility (ZTF, planned to succeed PTF in 2017), which will apply PTF's current resolution and sensitivity over a 47-deg^2 FOV
Processing Images from the Zwicky Transient Facility
The Zwicky Transient Facility is a new robotic-observing program, in which a
newly engineered 600-MP digital camera with a pioneeringly large field of view,
47~square degrees, will be installed into the 48-inch Samuel Oschin Telescope
at the Palomar Observatory. The camera will generate ~petabyte of raw
image data over three years of operations. In parallel related work, new
hardware and software systems are being developed to process these data in real
time and build a long-term archive for the processed products. The first public
release of archived products is planned for early 2019, which will include
processed images and astronomical-source catalogs of the northern sky in the
and bands. Source catalogs based on two different methods will be
generated for the archive: aperture photometry and point-spread-function
fitting.Comment: 6 pages, 4 figures, submitted to RTSRE Proceedings (www.rtsre.org
Evaluation of a Quantitative Real-Time PCR Assay to Measure HIV-Specific Mucosal CD8+ T Cell Responses in the Cervix
Several candidate HIV vaccines aim to induce virus-specific cellular immunity particularly in the genital tract, typically the initial site of HIV acquisition. However, standardized and sensitive methods for evaluating HIV-specific immune responses at the genital level are lacking. Therefore we evaluated real-time quantitative PCR (qPCR) as a potential platform to measure these responses. Ξ²-Actin and GAPDH were identified as the most stable housekeeping reference genes in peripheral blood mononuclear cells (PBMCs) and cervical mononuclear cells (CMCs) respectively and were used for normalizing transcript mRNA expression. HIV-specific cellular T cell immune responses to a pool of optimized CD8+ HIV epitopes (HIV epitope pool) and Staphylococcal enterotoxin B (SEB) superantigen control were assayed in HIV infected PBMC by qPCR, with parallel assessment of cytokine protein production. Peak HIV-specific mRNA expression of IFNΞ³, IL-2 and TNFΞ± occurred after 3, 5 and 12 hours respectively. PBMCs were titrated to cervical appropriate cell numbers to determine minimum required assay input cell numbers; qPCR retained sensitivity with input of at least 2.5Γ104 PBMCs. This optimized qPCR assay was then used to assess HIV-specific cellular T cell responses in cytobrush-derived cervical T cells from HIV positive individuals. SEB induced IFNΞ³ mRNA transcription was detected in CMCs and correlated positively with IFNΞ³ protein production. However, qPCR was unable to detect HIV-induced cytokine mRNA production in the cervix of HIV-infected women despite robust detection of gene induction in PBMCs. In conclusion, although qPCR can be used to measure ex vivo cellular immune responses to HIV in blood, HIV-specific responses in the cervix may fall below the threshold of qPCR detection. Nonetheless, this platform may have a potential role in measuring mitogen-induced immune responses in the genital tract
Targeted knock-down of miR21 primary transcripts using snoMEN vectors induces apoptosis in human cancer cell lines
We have previously reported an antisense technology, 'snoMEN vectors', for targeted knock-down of protein coding mRNAs using human snoRNAs manipulated to contain short regions of sequence complementarity with the mRNA target. Here we characterise the use of snoMEN vectors to target the knock-down of micro RNA primary transcripts. We document the specific knock-down of miR21 in HeLa cells using plasmid vectors expressing miR21-targeted snoMEN RNAs and show this induces apoptosis. Knock-down is dependent on the presence of complementary sequences in the snoMEN vector and the induction of apoptosis can be suppressed by over-expression of miR21. Furthermore, we have also developed lentiviral vectors for delivery of snoMEN RNAs and show this increases the efficiency of vector transduction in many human cell lines that are difficult to transfect with plasmid vectors. Transduction of lentiviral vectors expressing snoMEN targeted to pri-miR21 induces apoptosis in human lung adenocarcinoma cells, which express high levels of miR21, but not in human primary cells. We show that snoMEN-mediated suppression of miRNA expression is prevented by siRNA knock-down of Ago2, but not by knock-down of Ago1 or Upf1. snoMEN RNAs colocalise with Ago2 in cell nuclei and nucleoli and can be co-immunoprecipitated from nuclear extracts by antibodies specific for Ago2
VAST: An ASKAP Survey for Variables and Slow Transients
The Australian Square Kilometre Array Pathfinder (ASKAP) will give us an
unprecedented opportunity to investigate the transient sky at radio
wavelengths. In this paper we present VAST, an ASKAP survey for Variables and
Slow Transients. VAST will exploit the wide-field survey capabilities of ASKAP
to enable the discovery and investigation of variable and transient phenomena
from the local to the cosmological, including flare stars, intermittent
pulsars, X-ray binaries, magnetars, extreme scattering events, interstellar
scintillation, radio supernovae and orphan afterglows of gamma ray bursts. In
addition, it will allow us to probe unexplored regions of parameter space where
new classes of transient sources may be detected. In this paper we review the
known radio transient and variable populations and the current results from
blind radio surveys. We outline a comprehensive program based on a multi-tiered
survey strategy to characterise the radio transient sky through detection and
monitoring of transient and variable sources on the ASKAP imaging timescales of
five seconds and greater. We also present an analysis of the expected source
populations that we will be able to detect with VAST.Comment: 29 pages, 8 figures. Submitted for publication in Pub. Astron. Soc.
Australi
Finding Anomalous Periodic Time Series: An Application to Catalogs of Periodic Variable Stars
Catalogs of periodic variable stars contain large numbers of periodic
light-curves (photometric time series data from the astrophysics domain).
Separating anomalous objects from well-known classes is an important step
towards the discovery of new classes of astronomical objects. Most anomaly
detection methods for time series data assume either a single continuous time
series or a set of time series whose periods are aligned. Light-curve data
precludes the use of these methods as the periods of any given pair of
light-curves may be out of sync. One may use an existing anomaly detection
method if, prior to similarity calculation, one performs the costly act of
aligning two light-curves, an operation that scales poorly to massive data
sets. This paper presents PCAD, an unsupervised anomaly detection method for
large sets of unsynchronized periodic time-series data, that outputs a ranked
list of both global and local anomalies. It calculates its anomaly score for
each light-curve in relation to a set of centroids produced by a modified
k-means clustering algorithm. Our method is able to scale to large data sets
through the use of sampling. We validate our method on both light-curve data
and other time series data sets. We demonstrate its effectiveness at finding
known anomalies, and discuss the effect of sample size and number of centroids
on our results. We compare our method to naive solutions and existing time
series anomaly detection methods for unphased data, and show that PCAD's
reported anomalies are comparable to or better than all other methods. Finally,
astrophysicists on our team have verified that PCAD finds true anomalies that
might be indicative of novel astrophysical phenomena
Small Near-Earth Asteroids in the Palomar Transient Factory Survey: a Real-Time Streak-detection System
Near-Earth asteroids (NEAs) in the 1β100 meter size range are estimated to be ~1,000 times more numerous than the ~15,000 currently cataloged NEAs, most of which are in the 0.5β10 kilometer size range. Impacts from 10β100 meter size NEAs are not statistically life-threatening, but may cause significant regional damage, while 1β10 meter size NEAs with low velocities relative to Earth are compelling targets for space missions. We describe the implementation and initial results of a real-time NEA-discovery system specialized for the detection of small, high angular rate (visually streaked) NEAs in Palomar Transient Factory (PTF) images. PTF is a 1.2-m aperture, 7.3 deg^2 field of view (FOV) optical survey designed primarily for the discovery of extragalactic transients (e.g., supernovae) in 60-second exposures reaching ~20.5 visual magnitude. Our real-time NEA discovery pipeline uses a machine-learned classifier to filter a large number of false-positive streak detections, permitting a human scanner to efficiently and remotely identify real asteroid streaks during the night. Upon recognition of a streaked NEA detection (typically within an hour of the discovery exposure), the scanner triggers follow-up with the same telescope and posts the observations to the Minor Planet Center for worldwide confirmation. We describe our 11 initial confirmed discoveries, all small NEAs that passed 0.3β15 lunar distances from Earth. Lastly, we derive useful scaling laws for comparing streaked-NEA-detection capabilities of different surveys as a function of their hardware and survey-pattern characteristics. This work most directly informs estimates of the streak-detection capabilities of the Zwicky Transient Facility (ZTF, planned to succeed PTF in 2017), which will apply PTF's current resolution and sensitivity over a 47-deg^2 FOV
Flash Spectroscopy: Emission Lines from the Ionized Circumstellar Material Around <10-Day-Old Type II Supernovae
The American Astronomical Society. All rights reserved.Supernovae (SNe) embedded in dense circumstellar material (CSM) may show prominent emission lines in their early-time spectra (β€10 days after the explosion), owing to recombination of the CSM ionized by the shock-breakout flash. From such spectra ("flash spectroscopy"), we can measure various physical properties of the CSM, as well as the mass-loss rate of the progenitor during the year prior to its explosion. Searching through the Palomar Transient Factory (PTF and iPTF) SN spectroscopy databases from 2009 through 2014, we found 12 SNe II showing flash-ionized (FI) signatures in their first spectra. All are younger than 10 days. These events constitute 14% of all 84 SNe in our sample having a spectrum within 10 days from explosion, and 18% of SNe II observed at ages <5 days, thereby setting lower limits on the fraction of FI events. We classified as "blue/featureless" (BF) those events having a first spectrum that is similar to that of a blackbody, without any emission or absorption signatures. It is possible that some BF events had FI signatures at an earlier phase than observed, or that they lack dense CSM around the progenitor. Within 2 days after explosion, 8 out of 11 SNe in our sample are either BF events or show FI signatures. Interestingly, we found that 19 out of 21 SNe brighter than an absolute magnitude MR = -18.2 belong to the FI or BF groups, and that all FI events peaked above MR = -17.6 mag, significantly brighter than average SNe II
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