183 research outputs found
Methods to break seed dormancy of Astragalus cyclophyllon
The aim of this study was to enhance the germination rate of Astragalus cyclophyllon seeds which have a very low germination rate under normal conditions. The seeds were soaked for 72 h in 100, 200, 300,400 and 500 ppm gibberellic acid (GA3) solution, H2SO4 concentrations (50 and 98%) at two treatment times (5 and 10 min) and 60, 80 and 100ºC hot water at two treatment times (5 and 10 min) beforeplacing in Petri dishes. The fresh seeds (non-stratified) of A. cyclophyllon had 55% germination. Analysis of variance indicated that both GA3 and H2SO4 concentrations had significant effects on seed germination and final germination percentage. The highest germination percentage (81%) was obtained when the seeds were treated with 500 ppm GA3. The results showed that hot water treatments are not useful methods for breaking the seeds dormancy
A Distributed Transaction and Accounting Model for Digital Ecosystem Composed Services
This paper addresses two known issues for dynamically
composed services in digital ecosystems. The first
issue is that of efficient distributed transaction management.
The conventional view of transactions is unsuitable as the local
autonomy of the participants is vital for the involvement of
SMEs. The second issue is that of charging for such distributed
transactions, where there will often be dynamically created
services whose composition is not known in advance and might
involve parts of different transactions. The paper provides solutions
for both of these issues, which can be combined to provide
for a unified approach to transaction management and
accounting of dynamically composed services in digital ecosystems
Long-running Transactions: Semantics, schemas, implementation
In this paper we describe a formal model for the distributed coordination of long-running transactions in a Digital Ecosystem for business, involving Small and Medium Enterprises (SMEs). The proposed non-interleaving model of interaction-based service composition allows for communication between internal activities of transactions. The formal semantics of the various modes of service composition are represented by standard xml schemas. The current implementation framework uses suitable asynchronous message passing techniques and reflects the design decisions of the proposed model for distributed transactions in digital ecosystems
Diagnostic value of serum HER2 levels in breast cancer: a systematic review and meta-analysis.
BACKGROUND:Measurement of serum human epidermal growth factor receptor-2 (HER-2/neu) levels might play an essential role as a diagnostic/screening marker for the early selection of therapeutic approaches and predict prognosis in breast cancer patients. We aimed to undertake a systematic review and meta-analysis focusing on the diagnostic/screening value of serum HER-2 levels in comparison to routine methods. METHODS:We performed a systematic search via PubMed, Scopus, Cochrane-Library, and Web of Science databases for human diagnostic studies reporting the levels of serum HER-2 in breast cancer patients, which was confirmed using the histopathological examination. Meta-analyses were carried out for sensitivity, specificity, accuracy, area under the ROC curve (AUC), positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio (PLR), and negative likelihood ratio (NLR). RESULTS:Fourteen studies entered into this investigation. The meta-analysis indicated the low sensitivity for serum HER2 levels (Sensitivity: 53.05, 95%CI 40.82-65.28), but reasonable specificity of 79.27 (95%CI 73.02-85.51), accuracy of 72.06 (95%CI 67.04-77.08) and AUC of 0.79 (95%CI 0.66-0.92). We also found a significant differences for PPV (PPV: 56.18, 95%CI 44.16-68.20), NPV (NPV: 76.93, 95%CI 69.56-84.31), PLR (PLR: 2.10, 95%CI 1.69-2.50) and NLR (NLR: 0.58, 95%CI 0.44-0.71). CONCLUSION:Our findings revealed that although serum HER-2 levels showed low se nsitivity for breast cancer diagnosis, its specificity, accuracy and AUC were reasonable. Hence, it seems that the measurement of serum HER-2 levels can play a significant role as a verification test for initial negative screening test results, especially in low-income regions due to its cost-effectiveness and ease of implementation
Self-inflicted penetrating eye injuries using a razor blade: Case report
<p>Abstract</p> <p>Background</p> <p>A 23 year old white male with a history of social and behavioural problems attempted to blind himself chemically, with alcohol, and mechanically, with a razor blade.</p> <p>Methods</p> <p>Observational case report of a patient who self-inflicted bilateral scleral lacerations with a razor blade, after losing his job.</p> <p>Results</p> <p>The patient sustained bilateral inferior scleral perforations, with hypotony and a right traumatic cataract. He received urgent surgical repair, and prophylactic antibiotics. There were no retinal breaks or detachments. He later underwent successful cataract surgery to the right eye.</p> <p>Conclusion</p> <p>Self-inflicted ocular injury may be possible in non-psychotic patients, as a situational response to a life event. Urgent repair can completely restore vision in some cases. Referral for psychiatric counseling is mandatory.</p
Portable, Battery-Operated, Low-Cost, Bright Field and Fluorescence Microscope
This study describes the design and evaluation of a portable bright-field and fluorescence microscope that can be manufactured for $240 USD. The microscope uses a battery-operated LED-based flashlight as the light source and achieves a resolution of 0.8 µm at 1000× magnification in fluorescence mode. We tested the diagnostic capability of this new instrument to identify infections caused by the human pathogen, Mycobacterium tuberculosis. Sixty-four direct, decontaminated, and serially diluted smears were prepared from sputa obtained from 19 patients suspected to have M. tuberculosis infection. Slides were stained with auramine orange and evaluated as being positive or negative for M. tuberculosis with both the new portable fluorescence microscope and a laboratory grade fluorescence microscope. Concordant results were obtained in 98.4% of cases. This highly portable, low cost, fluorescence microscope may be a useful diagnostic tool to expand the availability of M. tuberculosis testing at the point-of-care in low resource settings
Iodine Atoms: A New Molecular Feature for the Design of Potent Transthyretin Fibrillogenesis Inhibitors
The thyroid hormone and retinol transporter protein known as transthyretin (TTR) is in the origin of one of the 20 or so known amyloid diseases. TTR self assembles as a homotetramer leaving a central hydrophobic channel with two symmetrical binding sites. The aggregation pathway of TTR into amiloid fibrils is not yet well characterized but in vitro binding of thyroid hormones and other small organic molecules to TTR binding channel results in tetramer stabilization which prevents amyloid formation in an extent which is proportional to the binding constant. Up to now, TTR aggregation inhibitors have been designed looking at various structural features of this binding channel others than its ability to host iodine atoms. In the present work, greatly improved inhibitors have been designed and tested by taking into account that thyroid hormones are unique in human biochemistry owing to the presence of multiple iodine atoms in their molecules which are probed to interact with specific halogen binding domains sitting at the TTR binding channel. The new TTR fibrillogenesis inhibitors are based on the diflunisal core structure because diflunisal is a registered salicylate drug with NSAID activity now undergoing clinical trials for TTR amyloid diseases. Biochemical and biophysical evidence confirms that iodine atoms can be an important design feature in the search for candidate drugs for TTR related amyloidosis
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Detection of cosmic structures using the bispectrum phase. II. First results from application to cosmic reionization using the Hydrogen Epoch of Reionization Array
Characterizing the epoch of reionization (EoR) at via the
redshifted 21 cm line of neutral Hydrogen (HI) is critical to modern
astrophysics and cosmology, and thus a key science goal of many current and planned low-frequency radio telescopes. The primary challenge to detecting this signal is the overwhelmingly bright foreground emission at these frequencies, placing stringent requirements on the knowledge of the instruments and inaccuracies in analyses. Results from these experiments have largely been limited not by thermal sensitivity but by systematics, particularly caused by the inability to calibrate the instrument to high accuracy. The interferometric bispectrum phase is immune to antenna-based calibration and errors therein, and presents an independent alternative to detect the EoR HI fluctuations while largely avoiding calibration systematics. Here, we provide a demonstration of this technique on a subset of data from the Hydrogen Epoch of Reionization Array (HERA) to place approximate constraints on the IGM brightness temperature. From this limited data, at we infer "" upper limits on the IGM brightness temperature to be "pseudo" mK at Mpc (data-limited) and
"pseudo" mK at
Mpc (noise-limited). The "pseudo" units denote only an approximate and not an exact correspondence to the actual distance scales and brightness temperatures. By propagating models in parallel to the data analysis, we confirm that the dynamic range required to separate the cosmic HI signal from the foregrounds is similar to that in standard approaches, and the power spectrum of the bispectrum phase is still data-limited (at dynamic range) indicating scope for further improvement in sensitivity as the array build-out continues
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Detection of cosmic structures using the bispectrum phase. II. First results from application to cosmic reionization using the Hydrogen Epoch of Reionization Array
Characterizing the epoch of reionization (EoR) at via the
redshifted 21 cm line of neutral Hydrogen (HI) is critical to modern
astrophysics and cosmology, and thus a key science goal of many current and planned low-frequency radio telescopes. The primary challenge to detecting this signal is the overwhelmingly bright foreground emission at these frequencies, placing stringent requirements on the knowledge of the instruments and inaccuracies in analyses. Results from these experiments have largely been limited not by thermal sensitivity but by systematics, particularly caused by the inability to calibrate the instrument to high accuracy. The interferometric bispectrum phase is immune to antenna-based calibration and errors therein, and presents an independent alternative to detect the EoR HI fluctuations while largely avoiding calibration systematics. Here, we provide a demonstration of this technique on a subset of data from the Hydrogen Epoch of Reionization Array (HERA) to place approximate constraints on the IGM brightness temperature. From this limited data, at we infer "" upper limits on the IGM brightness temperature to be "pseudo" mK at Mpc (data-limited) and
"pseudo" mK at
Mpc (noise-limited). The "pseudo" units denote only an approximate and not an exact correspondence to the actual distance scales and brightness temperatures. By propagating models in parallel to the data analysis, we confirm that the dynamic range required to separate the cosmic HI signal from the foregrounds is similar to that in standard approaches, and the power spectrum of the bispectrum phase is still data-limited (at dynamic range) indicating scope for further improvement in sensitivity as the array build-out continues
Imaging and Modeling Data from the Hydrogen Epoch of Reionization Array
We analyze data from the Hydrogen Epoch of Reionization Array. This is the
third in a series of papers on the closure phase delay-spectrum technique
designed to detect the HI 21cm emission from cosmic reionization. We present
the details of the data and models employed in the power spectral analysis, and
discuss limitations to the process. We compare images and visibility spectra
made with HERA data, to parallel quantities generated from sky models based on
the GLEAM survey, incorporating the HERA telescope model. We find reasonable
agreement between images made from HERA data, with those generated from the
models, down to the confusion level. For the visibility spectra, there is broad
agreement between model and data across the full band of MHz. However,
models with only GLEAM sources do not reproduce a roughly sinusoidal spectral
structure at the tens of percent level seen in the observed visibility spectra
on scales MHz on 29 m baselines. We find that this structure is
likely due to diffuse Galactic emission, predominantly the Galactic plane,
filling the far sidelobes of the antenna primary beam. We show that our current
knowledge of the frequency dependence of the diffuse sky radio emission, and
the primary beam at large zenith angles, is inadequate to provide an accurate
reproduction of the diffuse structure in the models. We discuss implications
due to this missing structure in the models, including calibration, and in the
search for the HI 21cm signal, as well as possible mitigation techniques
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