Progression and CSF Inflammation after Eradication of Oligoclonal Bands in an MS Patient Treated with Allogeneic Hematopoietic Cell Transplantation for Follicular Lymphoma

Background: Allogeneic hematopoietic cell transplantation (allo-HCT) has been proposed as treatment for multiple sclerosis (MS) and other autoimmune diseases. Aims: To describe the effects of allo-HCT on the course of MS in a 49-year-old woman with longstanding progressive MS who was treated with allo-HCT for follicular lymphoma. Methods: Non-myeloablative conditioning allo-HCT, examination for IgG oligoclonal bands and measurement of CXCL13 and matrix metalloproteinase-9 (MMP-9) concentration in the cerebrospinal fluid (CSF). Results: Despite the disappearance of oligoclonal bands in CSF, disease progression and CSF inflammation was observed. Conclusions: We hypothesize that CXCL13 and MMP-9 detected in CSF may reflect ongoing, pathogenic immune activation even after the eradication of intrathecal IgG synthesis. This suggests that progressive MS may depend more on innate than on adaptive immune activation

An applied methodology for stakeholder identification in transdisciplinary research

In this paper we present a novel methodology for identifying stakeholders for the purpose of engaging with them in transdisciplinary, sustainability research projects. In transdisciplinary research, it is important to identify a range of stakeholders prior to the problem-focussed stages of research. Early engagement with diverse stakeholders creates space for them to influence the research process, including problem definition, from the start. However, current stakeholder analysis approaches ignore this initial identification process, or position it within the subsequent content-focussed stages of research. Our methodology was designed as part of a research project into a range of soil threats in seventeen case study locations throughout Europe. Our methodology was designed to be systematic across all sites. It is based on a snowball sampling approach that can be implemented by researchers with no prior experience of stakeholder research, and without requiring significant financial or time resources. It therefore fosters transdisciplinarity by empowering physical scientists to identify stakeholders and understand their roles. We describe the design process and outcomes, and consider their applicability to other research projects. Our methodology therefore consists of a two-phase process of design and implementation of an identification questionnaire. By explicitly including a design phase into the process, it is possible to tailor our methodology to other research projects

Use of farmers' indicators to evaluate the sustainability of cropping systems on sloping land in Yunnan Province, China.

Diversity in the biophysical and socio-economic attributes of agricultural systems makes them uniquely niche based. Farmers are expert in local biophysical and socio-economic situations and can contribute in developing pragmatic indicators of agro-environmental development. During evaluation of an agricultural research project in Yunnan, China, local farmers were capable of evaluating the effects of modified technologies on existing cropping systems and discussed their attitudes to the interventions using their own indicators. Farmers' response can be grouped into seven major aspects: i) effects on income, ii) effects on production resources, iii) effects on crop management, iv) existing local knowledge about the technology, v) availability of inputs, vi) access to information, and vii) socio-economic conditions of farming households. Farmers concluded that environmental conditions in the experimental catchment in comparison to an adjacent untreated catchment were better in terms of soil and water losses, vegetation cover and natural resources, infrastructures and catchment management, use of environmentally-friendly technologies, and crop productivity. Success in soil and water conservation programmes depends on the efforts of the farmers and other local users and their greater involvement helps to identify more pragmatic indicators. Furthermore, it increases ownership of the programme, enhances interactions with the project scientists, increases farmers' awareness of agro-environmental problems and their possible consequences. These development will enable scientists to develop better targeted interventions and increase the likelihood of adoption of tested technologies by local communities. The use of paired adjacent catchments improved evaluation activities and is proposed as good practice for future catchment improvement programmes