Topography induced spatial variations in diurnal cycles of assimilation and latent heat of Mediterranean forest

The aim of this study is to explain topography induced spatial variations in the diurnal cycles of assimilation and latent heat of Mediterranean forest. Spatial variations of the fluxes are caused by variations in weather conditions and in vegetation characteristics. Weather conditions reflect short-term effects of climate, whereas vegetation characteristics, through adaptation and acclimation, long-term effects of climate. In this study measurements of plant physiology and weather conditions are used to explain observed differences in the fluxes. A model is used to study which part of the differences in the fluxes is caused by weather conditions and which part by vegetation characteristics. Data were collected at four experimental sub-Mediterranean deciduous forest plots in a heterogeneous terrain with contrasting aspect, soil water availability, humidity and temperature. We used a sun-shade model to scale fluxes from leaf to canopy, and calculated the canopy energy balance. Parameter values were derived from measurements of light interception, leaf chamber photosynthesis, leaf nitrogen content and ¹³C isotope discrimination in leaf material. Leaf nitrogen content is a measure of photosynthetic capacity, and ¹³C isotope discrimination of water use efficiency. For validation, sap-flux based measurements of transpiration were used. The model predicted diurnal cycles of transpiration and stomatal conductance, both their magnitudes and differences in afternoon stomatal closure between slopes of different aspect within the confidence interval of the validation data. Weather conditions mainly responsible for the shape of the diurnal cycles, and vegetation parameters for the magnitude of the fluxes. Although the data do not allow for a quantification of the two effects, the differences in vegetation parameters and weather among the plots and the sensitivity of the fluxes to them suggest that the diurnal cycles were more strongly affected by spatial variations in vegetation parameters than by meteorological variables. This indicates that topography induced variations in vegetation parameters are of equal importance to the fluxes as topography induced variations in radiation, humidity and temperature

Yeast biodiversity in vineyard environments is increased by human intervention

One hundred and five grape samples were collected during two consecutive years from 33 locations on seven oceanic islands of the Azores Archipelago. Grape samples were obtained from vineyards that were either abandoned or under regular cultivation involving common viticultural interventions, to evaluate the impact of regular human intervention on grape yeast biota diversity in vineyards. A total of 3150 yeast isolates were obtained and 23 yeast species were identified. The predominant species were Hanseniaspora uvarum, Pichia terricola, Starmerella bacillaris and Issatchenkia hanoiensis. The species Barnettozyma californica, Candida azymoides and Pichia cecembensis were reported in grapes or wine-associated environments for the first time. A higher biodiversity was found in active vineyards where regular human intervention takes place (Shannon index: 1.89 and 1.53 in the first and second years, respectively) when compared to the abandoned ones (Shannon index: 0.76 and 0.31). This finding goes against the assumptions that human intervention can destroy biodiversity and lead to homogeneity in the environment. Biodiversity indices were considerably lower in the year with the heaviest rainfall. This study is the first to report on the grape yeast communities from several abandoned vineyards that have undergone no human intervention.Joao Drumonde Neves is the recipient of a fellowship of the Azorean Government (M321/006/F/2008) and PROEMPREGO. This work was supported by the strategic programme UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569) funded by national funds through the FCT I.P. and by the ERDF through the COMPETE2020 - Programa Operacional Competitividade e Internacionalizacao (POCI), and by national funds through FCT by the projects FCOMP-01-0124-008775, PTDC/AGR-ALI/103392/2008 and PTDC/AGR-ALI/121062/2010.info:eu-repo/semantics/publishedVersio

Metal Bioavailability in the Sava River Water

Metals present one of the major contamination problems for freshwater systems, such as the Sava River, due to their high toxicity, persistence, and tendency to accumulate in sediment and living organisms. The comprehensive assessment of the metal bioavailability in the Sava River encompassed the analyses of dissolved and DGT-labile metal species of nine metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) in the river water, as well as the evaluation of the accumulation of five metals (Cd, Cu, Fe, Mn, and Zn) in three organs (liver, gills, and gastrointestinal tissue) of the bioindicator organism, fish species European chub (Squalius cephalus L.).This survey was conducted mainly during the year 2006, in two sampling campaigns, in April/May and September, as periods representative for chub spawning and post-spawning. Additionally, metal concentrations were determined in the intestinal parasites acanthocephalans, which are known for their high affinity for metal accumulation. Metallothionein concentrations were also determined in three chub organs, as a commonly applied biomarker of metal exposure. Based on the metal concentrations in the river water, the Sava River was defined as weakly contaminated and mainly comparable with unpolluted rivers, which enabled the analyses of physiological variability of metal and metallothionein concentrations in the chub organs, as well as the establishment of their constitutive levels

Electrochemical methods for speciation of trace elements in marine waters. Dynamic aspects

The contribution of electrochemical methods to the knowledge of dynamic speciation of toxic trace elements in marine waters is critically reviewed. Due to the importance of dynamic considerations in the interpretation of the electrochemical signal, the principles and recent developments of kinetic features in the interconversion of metal complex species will be presented. As dynamic electrochemical methods, only stripping techniques (anodic stripping voltammetry and stripping chronopotentiometry) will be used because they are the most important for the determination of trace elements. Competitive ligand ex- change-adsorptive cathodic stripping voltammetry, which should be considered an equilibrium technique rather than a dynamic method, will be also discussed because the complexing parameters may be affected by some kinetic limitations if equilibrium before analysis is not attained and/or the flux of the adsorbed complex is in fluenced by the lability of the natural complexes in the water sample. For a correct data interpretation and system characterization the comparison of results obtained from different techniques seems essential in the articulation of a serious discussion of their meaning