2,189 research outputs found

    Effects of soil and canopy characteristics on microwave backscattering of vegetation

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    A frequency modulated continuous wave C-band (4.8 GHz) scatterometer was mounted on an aerial lift truck and backscatter coefficients of corn were acquired as functions of polarizations, view angles, and row directions. As phytomass and green leaf area index increased, the backscatter also increased. Near anthesis when the canopies were fully developed, the major scattering elements were located in the upper 1 m of the 2.8 m tall canopy and little backscatter was measured below that level. C-band backscatter data could provide information to monitor vegetation at large view zenith angles

    Estimating scattered and absorbed radiation in plant canopies by remote sensing

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    Several research avenues are summarized. The relationships of canopy characteristics to multispectral reflectance factors of vegetation are reviewed. Several alternative approaches for incorporating spectrally derived information into plant models are discussed, using corn as the main example. A method is described and evaluated whereby a leaf area index is estimated from measurements of radiation transmitted through plant canopies, using soybeans as an example. Albedo of a big bluestem grass canopy is estimated from 60 directional reflectance factor measurements. Effects of estimating albedo with substantially smaller subsets of data are evaluated

    Changes in murine anorectum signaling across the life course

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    Background: Increasing age is associated with an increase in the incidence of chronic constipation and fecal impaction. The contribution of the natural aging process to these conditions is not fully understood. This study examined the effects of increasing age on the function of the murine anorectum.Methods: The effects of increasing age on cholinergic, nitrergic, and purinergic signaling pathways in the murine anorectum were examined using classical organ bath assays to examine tissue function and electrochemical sensing to determine age‐related changes in nitric oxide and acetylcholine release.Key Results: Nitrergic relaxation increased between 3 and 6 months, peaked at 12 months and declined in the 18 and 24 months groups. These changes were in part explained by an age‐related decrease in nitric oxide (NO) release. Cholinergic signaling was maintained with age by an increase in acetylcholine (ACh) release and a compensatory decrease in cholinesterase activity. Age‐related changes in purinergic relaxation were qualitatively similar to nitrergic relaxation although the relaxations were much smaller. Increasing age did not alter the response of the anorectum smooth muscle to exogenously applied ACh, ATP, sodium nitroprusside or KCl. Similarly, there was no change in basal tension developed by the anorectum.Conclusions and Inferences: The decrease in nitrergic signaling with increasing age may contribute to the age‐related fecal impaction and constipation previously described in this model by partially obstructing defecation

    Characterization of vegetation by microwave and optical remote sensing

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    Two series of carefully controlled experiments were conducted. First, plots of important crops (corn, soybeans, and sorghum), prairie grasses (big bluestem, switchgrass, tal fescue, orchardgrass, bromegrass), and forage legumes (alfalfa, red clover, and crown vetch) were manipulated to produce wide ranges of phytomass, leaf area index, and canopy architecture. Second, coniferous forest canopies were simulated using small balsam fir trees grown in large pots of soil and arranged systematically on a large (5 m) platform. Rotating the platform produced many new canopies for frequency and spatial averaging of the backscatter signal. In both series of experiments, backscatter of 5.0 GHz (C-Band) was measured as a function of view angle and polarization. Biophysical measurements included leaf area index, fresh and dry phytomass, water content of canopy elements, canopy height, and soil roughness and moisture content. For a subset of the above plots, additional measurements were acquired to exercise microwave backscatter models. These measurements included size and shape of leaves, stems, and fruit and the probability density function of leaf and stem angles. The relationships of the backscattering coefficients and the biophysical properties of the canopies were evaluated using statistical correlations, analysis of variance, and regression analysis. Results from the corn density and balsam fir experiments are discussed and analyses of data from the other experiments are summarized

    Soybean canopy reflectance modeling data sets

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    Numerous mathematical models of the interaction of radiation with vegetation canopies have been developed over the last two decades. However, data with which to exercise and validate these models are scarce. During three days in the summer of 1980, experiments are conducted with the objective of gaining insight about the effects of solar illumination and view angles on soybean canopy reflectance. In concert with these experiment, extensive measurements of the soybean canopies are obtained. This document is a compilation of the bidirectional reflectance factors, agronomic, characteristics, canopy geometry, and leaf, stem, and pod optical properties of the soybean canopies. These data sets should be suitable for use with most vegetation canopy reflectance models

    Transparent and Flexible Thin Film Electroluminescent Devices Using HiTUS Deposition and Laser Processing Fabrication

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    Highly transparent thin film electroluminescent structures offering excellent switch on characteristics, high luminance and large break-down voltages have been deposited onto glass and flexible polymeric materials with no substrate heating using high target utilization sputtering. Deposition of ZnS:Mn as the active light emitting layer and Y2O3,Al2O3,Ta2O5, and HfO2 as dielectric materials arranged in single and multiple layer configurations were investigated. Devices incorporating Al2O3,HfO2 quadruple layers demonstrate the highest attainable luminance at low threshold voltage. Single pulse excimer laser irradiation of the phosphor layer prior to deposition of the top dielectric layer enhanced the luminance of the devices. The devices fabricated on glass and polymeric substrates exhibited a maximum luminance of 500 and 450 cdm−2 when driven at 270 VRMS and 220 VRMS, respectively, with a 1.0 kHz sine wave

    Identification and analysis of Single Nucleotide Polymorphisms (SNPs) in the mosquito Anopheles funestus, malaria vector

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    BACKGROUND: Single nucleotide polymorphisms (SNPs) are the most common source of genetic variation in eukaryotic species and have become an important marker for genetic studies. The mosquito Anopheles funestus is one of the major malaria vectors in Africa and yet, prior to this study, no SNPs have been described for this species. Here we report a genome-wide set of SNP markers for use in genetic studies on this important human disease vector. RESULTS: DNA fragments from 50 genes were amplified and sequenced from 21 specimens of An. funestus. A third of specimens were field collected in Malawi, a third from a colony of Mozambican origin and a third form a colony of Angolan origin. A total of 494 SNPs including 303 within the coding regions of genes and 5 indels were identified. The physical positions of these SNPs in the genome are known. There were on average 7 SNPs per kilobase similar to that observed in An. gambiae and Drosophila melanogaster. Transitions outnumbered transversions, at a ratio of 2:1. The increased frequency of transition substitutions in coding regions is likely due to the structure of the genetic code and selective constraints. Synonymous sites within coding regions showed a higher polymorphism rate than non-coding introns or 3' and 5'flanking DNA with most of the substitutions in coding regions being observed at the 3(rd )codon position. A positive correlation in the level of polymorphism was observed between coding and non-coding regions within a gene. By genotyping a subset of 30 SNPs, we confirmed the validity of the SNPs identified during this study. CONCLUSION: This set of SNP markers represents a useful tool for genetic studies in An. funestus, and will be useful in identifying candidate genes that affect diverse ranges of phenotypes that impact on vector control, such as resistance insecticide, mosquito behavior and vector competence

    Interstitial cell network volume is reduced in the terminal bowel of ageing mice

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    Ageing is associated with impaired neuromuscular function of the terminal gastrointestinal (GI) tract, which can result in chronic constipation, faecal impaction and incontinence. Interstitial cells of cajal (ICC) play an important role in regulation of intestinal smooth muscle contraction. However, changes in ICC volume with age in the terminal GI tract (the anal canal including the anal sphincter region and rectum)have not been studied. Here, the distribution, morphology and network volume of ICC in the terminal GI tract of 3‐to 4‐month‐old and 26‐to 28‐month‐old C57BL/6mice were investigated. ICC were identified by immunofluorescence labelling of wholemount preparations with an antibody against c‐Kit. ICC network volume was measured by software‐based 3D volume rendering of confocal Z stacks. A significant reduction in ICC network volume per unit volume of muscle was measured in aged animals. No age‐associated change in ICC morphology was detected. The thickness of the circular muscle layer of the anal sphincter region and rectum increased with age, while that in the distal colon decreased. These results suggest that ageing is associated with a reduction in the network volume of ICC in the terminal GI tract, which may influence the normal function of these regions
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