1,540 research outputs found
FDG-PET: a new diagnostic approach in hip prosthesis infection
Infection following hip arthroplasties can present a diagnostic challenge. No
test is 100 % sensitive and 100 % specific; this prospective study was undertaken
to evaluate the utility of FDG-PET imaging for diagnosing infected joint
replacements. 24 hip joint replacements were studied prospectively and we have
complete diagnoses with clinical signs and symptoms, laboratory test,
radiography, joint aspiration, radionuclide imaging including FDG-PET, and
histopathologic examination. 11 of 24 prostheses were infected. The sensitivity
and specificity of PET for detecting infection associated with prostheses were
64,3 % and 64,7 % respectively, in our hands. FDG imaging is not useful in
patients with suspected prosthetic infection like a screening tes
Impact of ambulatory blood pressure monitoring on reclassification of hypertension prevalence and control in older people in Spain
This is the submitted version of the following article: Impact of ambulatory blood pressure monitoring on reclassification of hypertension prevalence and control in older people in Spain, Journal of Clinical Hypertension 17.6:453-61 which has been published in final form at http://dx.doi.org/10.1111/jch.12525. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy wiley.com/WileyCDA/Section/id- 820227.htmlAmbulatory blood pressure monitoring (ABPM) accurately classifies blood pressure (BP) status but its impact on the prevalence and control of hypertension is little known. The authors conducted a cross-sectional study in 2012 among 1047 individuals 60 years and older from the follow-up of a population cohort in Spain. Three casual BP measurements and 24-hour ABPM were performed under standardized conditions. Approximately 68.8% patients were hypertensive based on casual BP (≥140/90 mm Hg or current BP medication use) and 62.1% based on 24-hour ABPM (≥130/80 mm Hg or current BP medication use) (P=.009). The proportion of patients with treatment-eligible hypertension who met BP goals increased from 37.4% based on the casual BP target to 54.1% based on the 24-hour BP target (absolute difference, 16.7%; P<.01). These results were consistent across alternative BP thresholds. Therefore, compared with casual BP, 24-hour ABPM led to a reduction in the proportion of older patients recommended for hypertension treatment and a substantial increase in the proportion of those with hypertension controlData collection was funded by Fondo de Investigación Sanitaria (FIS) grants 09/1626 and 12/1166 (Ministry of Health of Spain) and by the ‘Cátedra UAM de Epidemiología y Control del Riesgo Cardiovascular’. Specific funding for this analysis was obtained from FIS grant PI13/02321
Single-institution experience in clinical trials during the COVID-19 pandemic in Spain: Not so bad after all?
The impact of the COVID-19 outbreak in Spain during
March-April 2020 has been unbalanced throughout
the different regions of the country. The alarm status
defined by the government on March 14, and still in
place at the time of this writing, has transformed the
country in different perspectives, including care of
patients with cancer.1 In many centers, clinical trial
activity was suspended, because it was not considered
a priority under the health care challenge of the
COVID-19 pandemic.2 Nevertheless, experimental
therapy is the only and/or best therapeutic option for
many patients with cancer
Tumor necrosis factor-alpha inhibition reduces CXCL-8 levels but fails to prevent fibrin generation and does not improve outcome in a rabbit model of endotoxic shock
The effects of a monoclonal antibody (mAb) to tumor necrosis factor-alpha (TNF-alpha) were examined in a rabbit model of endotoxic shock. Intravenous administration of lipopolysaccharide (100 microg/kg/hr) for 6 hours (n = 11) increased TNF-alpha levels. Fibrinogen was partially consumed, and fibrin deposits were seen in kidney and lungs at 24 hours. Mortality at 24 hours was 64%. Levels of interleukin-8 (aka CXCL-8) were notably increased. Mean arterial pressure (MAP) and leukocyte counts decreased, whereas creatinine levels were enhanced. The anti-TNF-alpha mAb (20 mg/kg i.v. bolus + 5 mg/kg/h i.v. for the first 90 minutes) (n = 10) efficiently inhibited the TNF-activity. Rabbits exhibited lower CXCL-8 levels; MAP improved, the decrease in leukocyte counts was partially prevented and creatinine levels were lower, but fibrinogen, fibrin deposits in kidneys and lungs and mortality, 55%, were similar to the LPS group. Rabbits that did not survive exhibited lower fibrinogen levels, more fibrin in kidneys and lungs and higher CXCL-8 and creatinine levels than survivors, while there were no differences in TNF-alpha, MAP and leukocytes. Thus, the inhibition of TNF-alpha, although beneficial through lowering CXCL-8 levels, is not enough to improve the outcome, which could be partly due to the inability to prevent the fibrin deposits formation in kidneys and lungs
Analysis of the key features of the seismic actions due to the three main earthquakes of May 11, 2011 in Lorca, Spain
The seismic records are in general valuable information, especially in cases where damage in buildings has occurred. The main purpose of the present document is to describe the principal results of the analysis of features of ground motions due to the main three earthquakes that occurred in Spain on May 11, 2011. In this day the major earthquake had a magnitude of 5.1 Mw. This event triggered different levels of damage in numerous buildings in the city of Lorca located in southern Spain. Unfortunately, 9 persons died due mainly to the collapse of non-structural elements. We describe in the present paper the application of the software Seismograms Analyzer-e (SA-e) to perform the processing and the analysis of the seismic records obtained in five stations during the main three earthquakes on May 11 (the largest earthquake of magnitude 5.1 Mw, the precursor of magnitude 4.5 Mw, and the aftershock of magnitude 3.9 Mw). We also highlight the significant similitudes between the seismograms generated in the LOR station during these three earthquakes. Additionally, we determined the values of acceleration that occurred in the roof of the buildings of Lorca, because these values of acceleration contributed both to the damage of numerous buildings and the collapse of several parapets of some buildings. The analysis of these accelerations is relevant because the collapse of some parapets was the cause of the death of the 9 people that died during the main earthquake. For example, according to our study in the roof of a building with a fundamental period of 0.25 s the acceleration could have reached values near to 1.04 g. We also analyzed the potential of damage in function of the values of CAVSTD. Additionally, we determined hypothetical seismic forces for the design of parapets in buildings of Lorca considering the NCSE-02 normative, and the values of Sa based on the seismic records. We determined a significant difference between the seismic forces that could have been used to design the parapets of the buildings in the Lorca city and the forces determined according to the values of PGA that were proposed in a recent study of seismic hazard for Spain.The first author acknowledges to CONACYT, Barcelona Supercomputing Center, and to the Universidad Veracruzana by their contribution to the development of the present study. Complementary information about Seismograms Analyzer-e is available on the WEB page of SA-e2 .
.Peer ReviewedPostprint (published version
Use of colonoscopy as a primary screening test for colorectal cancer in average risk people
The use of colonoscopy as a primary screening test for colorectal
cancer (CRC) in average risk adults is a subject of controversy. Our primary
objective was to build a predictive model based on a few simple variables that
could be used as a guide for identifying average risk adults more suitable for
examination with colonoscopy as a primary screening test. METHODS: The prevalence
of advanced adenomas was assessed by primary screening colonoscopy in 2210
consecutive adults at least 40 yr old, without known risk factors for CRC. Age,
gender, and clinical and biochemical data were compared among people without
adenomas, those with non-advanced adenomas, and those with any advanced neoplasm.
A combined score to assess the risk of advanced adenomas was built with the
variables selected by multiple logistic regression analysis. RESULTS: Neoplastic
lesions were found in 617 subjects (27.9%), including 259 with at least one
neoplasm that was 10 mm or larger, villous, or with moderate-to-severe dysplasia,
and 11 with invasive cancers. Advanced lesions were more frequent among men,
older people, and those with a higher body mass index (BMI). These three
variables were independent predictors of advanced adenomas in multivariate
analysis. A score combining age, sex, and BMI was developed as a guide for
identifying individuals more suitable for screening colonoscopy. CONCLUSIONS:
Age, gender, and BMI can be used to build a simple score to select those average
risk adults who might be candidates for primary screening colonoscop
Diagnostic value of distal colonic polyps for prediction of advanced proximal neoplasia in an average-risk population undergoing screening colonoscopy
For colorectal cancer screening, the predictive value of distal
findings in the ascertainment of proximal lesions is not fully established. The
aims of this study were to assess distal findings as predictors of advanced
proximal neoplasia and to compare the predictive value of endoscopy alone vs.
combined endoscopic and histopathologic data. METHODS: Primary colonoscopy
screening was performed in 2210 consecutive, average-risk adults. Age, gender,
endoscopic (size, number of polyps), and histopathologic distal findings were
used as potential predictors of advanced proximal neoplasms (i.e., any adenoma >
or =1 cm in size, and/or with villous histology, and/or with severe dysplasia or
invasive cancer). Polyps were defined as distal if located in the descending
colon, the sigmoid colon, or the rectum. Those in other locations were designated
proximal. RESULTS: Neoplastic lesions, including 11 invasive cancers, were found
in 617 (27.9%) patients. Advanced proximal neoplasms without any distal adenoma
were present in 1.3% of patients. Of the advanced proximal lesions, 39% were not
associated with any distal polyp. Older age, male gender, and distal adenoma were
independent predictors of advanced proximal neoplasms. The predictive ability of
a model with endoscopic data alone did not improve after inclusion of
histopathologic data. In multivariate logistic regression analysis, the
predictive ability of models that use age, gender, and any combination of distal
findings was relatively low. The proportion of advanced proximal neoplasms
identified if any distal polyp was an indication for colonoscopy was only 62%.
CONCLUSIONS: A strategy in which colonoscopy is performed solely in patients with
distal colonic findings is not effective screening for the detection of advanced
proximal neoplasms in an average-risk populatio
Metal-Support Interactions and C1 Chemistry: Transforming Pt-CeO2into a Highly Active and Stable Catalyst for the Conversion of Carbon Dioxide and Methane
There is an ongoing search for materials which can accomplish the activation of two dangerous greenhouse gases like carbon dioxide and methane. In the area of C1 chemistry, the reaction between CO2 and CH4 to produce syngas (CO/H2), known as methane dry reforming (MDR), is attracting a lot of interest due to its green nature. On Pt(111), high temperatures must be used to activate the reactants, leading to a substantial deposition of carbon which makes this metal surface useless for the MDR process. In this study, we show that strong metal-support interactions present in Pt/CeO2(111) and Pt/CeO2 powders lead to systems which can bind CO2 and CH4 well at room temperature and are excellent and stable catalysts for the MDR process at moderate temperature (500 °C). The behavior of these systems was studied using a combination of in situ/operando methods (AP-XPS, XRD, and XAFS) which pointed to an active Pt-CeO2-x interface. In this interface, the oxide is far from being a passive spectator. It modifies the chemical properties of Pt, facilitating improved methane dissociation, and is directly involved in the adsorption and dissociation of CO2 making the MDR catalytic cycle possible. A comparison of the benefits gained by the use of an effective metal-oxide interface and those obtained by plain bimetallic bonding indicates that the former is much more important when optimizing the C1 chemistry associated with CO2 and CH4 conversion. The presence of elements with a different chemical nature at the metal-oxide interface opens the possibility for truly cooperative interactions in the activation of C-O and C-H bonds.Fil: Zhang, Feng. State University of New York. Stony Brook University; Estados UnidosFil: Gutiérrez, Ramón A.. Universidad Central de Venezuela; VenezuelaFil: Lustemberg, Pablo German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Consejo Superior de Investigaciones Científicas; EspañaFil: Liu, Zongyuan. Brookhaven National Laboratory; Estados UnidosFil: Rui, Ning. Brookhaven National Laboratory; Estados UnidosFil: Wu, Tianpin. Argonne National Laboratory; Estados UnidosFil: Ramírez, Pedro J.. Zoneca-cenex; México. Universidad Central de Venezuela; VenezuelaFil: Xu, Wenqian. Argonne National Laboratory; Estados UnidosFil: Idriss, Hicham. King Abdullah University of Science and Technology; Arabia SauditaFil: Ganduglia Pirovano, M. Verónica. Consejo Superior de Investigaciones Científicas; EspañaFil: Senanayake, Sanjaya D.. Brookhaven National Laboratory; Estados UnidosFil: Rodriguez, José A.. Brookhaven National Laboratory; Estados Unidos. State University of New York. Stony Brook University; Estados Unido
Identification and characterization of aspartyl-tRNA synthetase inhibitors against Mycobacterium tuberculosis by an integrated whole-cell target-based approach
Epigenetic Mechanisms Regulate MHC and Antigen Processing Molecules in Human Embryonic and Induced Pluripotent Stem Cells
Background
Human embryonic stem cells (hESCs) are an attractive resource for new therapeutic approaches that involve tissue regeneration. hESCs have exhibited low immunogenicity due to low levels of Mayor Histocompatibility Complex (MHC) class-I and absence of MHC class-II expression. Nevertheless, the mechanisms regulating MHC expression in hESCs had not been explored.
Methodology/Principal Findings
We analyzed the expression levels of classical and non-classical MHC class-I, MHC class-II molecules, antigen-processing machinery (APM) components and NKG2D ligands (NKG2D-L) in hESCs, induced pluripotent stem cells (iPSCs) and NTera2 (NT2) teratocarcinoma cell line. Epigenetic mechanisms involved in the regulation of these genes were investigated by bisulfite sequencing and chromatin immunoprecipitation (ChIP) assays. We showed that low levels of MHC class-I molecules were associated with absent or reduced expression of the transporter associated with antigen processing 1 (TAP-1) and tapasin (TPN) components in hESCs and iPSCs, which are involved in the transport and load of peptides. Furthermore, lack of β2-microglobulin (β2m) light chain in these cells limited the expression of MHC class I trimeric molecule on the cell surface. NKG2D ligands (MICA, MICB) were observed in all pluripotent stem cells lines. Epigenetic analysis showed that H3K9me3 repressed the TPN gene in undifferentiated cells whilst HLA-B and β2m acquired the H3K4me3 modification during the differentiation to embryoid bodies (EBs). Absence of HLA-DR and HLA-G expression was regulated by DNA methylation.
Conclusions/Significance
Our data provide fundamental evidence for the epigenetic control of MHC in hESCs and iPSCs. Reduced MHC class I and class II expression in hESCs and iPSCs can limit their recognition by the immune response against these cells. The knowledge of these mechanisms will further allow the development of strategies to induce tolerance and improve stem cell allograft acceptance
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