Effect of a commercial root growth enhancer on the turfgrass species "Lolium perenne" and the weed "Poa annua"

Se pretende comprobar si la aplicación de un enraizante durante la resiembra otoñal de la cespitosa “Lolium perenne” sobre las calles de los campos de golf conformados por “Cynodon dactylon”, favoreciese la competencia de “L. perenne” frente a Poa annua, mala hierba que germina precisamente en esta época. Se realizaron 2 ensayos iguales en cámara de crecimiento aplicando 10 dosis distintas del enraizante en ambas especies y se determinó al final del ensayo, tanto el crecimiento como el peso seco de la parte aérea y radical de ambas especies. Tan sólo en uno de los 2 ensayos el enraizante parece favorecer a la cespitosa frente a “P. annua” por lo que no puede concluirse categóricamente que el enraizante pueda ser un medio de control efectivo frente a la mala hierba.It is intended to check the performance of a root growth enhancer in order to promote “Lolium perenne” competiveness over “Poa annua”, during the overseeding cultural practice on “Cynodon dactylon” golf course fairway, as “P. annua” naturally germinates at the same time. Two equal laboratory experiments were conducted testing 10 doses of the root enhancer in both species. At the end of the experiments seedling growth and root and leaf dry weight were determined for both species. The tested product only promotes “L. perenne” competiveness in one experiment, therefore it cannot be concluded the root enhancer could be an effective weed control method

Analysis of Geometrical Relationships and Friction Losses in Small-Diameter Lay-Flat Polyethylene Pipes

[EN] The use of lay-flat polyethylene pipes to irrigate horticultural crops has been receiving widespread attention in the last decade, due to the significant improvements in their hydraulic performance, their potentially high application efficiency, and their limited installation costs. However, even if hydraulic design procedures for conventional microirrigation systems are fairly well established, there is still the need to know how different pipe-wall thicknesses of lay-flat pipes can affect the pipe geometry under different operating pressures as well as the related consequences on friction losses. This paper, after comparing two different procedures (caliper and photographic) to assess the geometry of lay-flat polyethylene pipes under different operating pressures, analyzes the friction losses per unit of pipe length, J, in order to identify and to assess a procedure for their evaluation. Hydrostatic tests, initially carried out on pipes with wall thicknesses of 6, 8, and 10 thousandth of an inch (mil), evidenced that the pipe dimensions measured with both methods are quite similar, despite the generally higher standard deviations characterizing caliper measurements when compared to photographic method. Tests allowed to verify that most of the changes in pipe dimensions occur within a range of pressure from 0 kPa to about 30 kPa, with pipe horizontal width and vertical height quite similar at higher pressures and pipes have a tendency to become circular. Additionally, due to the elasticity of the material, over a certain limit of water pressure, both the pipe dimensions tend to rise, with a trend depending on pipe thickness. According to the experimental data, the relationships between pipe effective diameter and water pressure were then determined for the three considered pipes. Moreover, based on measured friction losses and pipe effective diameters, it was confirmed that the relationship between the Darcy-Weisbach friction factor, f, and the Reynolds number, R, can be described by a power equation in which, by assuming a value of -0.25 for the exponent, it results a coefficient c = 0.285, lower than the theoretical. For the three investigated pipes the errors associated to estimated J were finally evaluated by considering (1) the experimental relationships between friction factor and Reynolds number as well as between pipe diameter and operating pressure (Case A); (2) the same value of c, but pipe effective diameters of 16.20, 16.10, and 15.85 mm corresponding to p = p(lim) (Case B); (3) the standard procedure, with a value of c = 0.302 and the pipe diameter equal to 16.10 mm, as suggested by the manufacturer. The results evidenced that suitable estimations of J need to account for the variations of the pipe effective diameter with water pressure. On the other hand, incorrect values of pipe diameter combined with inexact values of the friction factor generate inaccurate estimations of friction losses, with unavoidable consequences in pipe design. (C) 2015 American Society of Civil Engineers.The research was cofinanced by Universita di Palermo (FFR 2011) and Ministero dell'Istruzione, dell'Universita e della Ricerca (PRIN 2010). All the authors setup the research and discussed the results. V. Alagna and D. Autovino carried out the experimental measurements and G. Provenzano wrote the paper. A special thank to the Committee for International Relations Office (CORI) of University of Palermo to support the research cooperation with the University of Valencia.Provenzano, G.; Alagna, V.; Autovino, D.; Manzano Juarez, J.; Rallo, G. (2016). Analysis of Geometrical Relationships and Friction Losses in Small-Diameter Lay-Flat Polyethylene Pipes. Journal of Irrigation and Drainage Engineering. 142(2):1-9. https://doi.org/10.1061/(ASCE)IR.1943-4774.0000958S19142

Epigenetics, stem cells, and autophagy: Exploring a path involving miRNA

MiRNAs, a small family of non-coding RNA, are now emerging as regulators of stem cell pluripotency, differentiation, and autophagy, thus controlling stem cell behavior. Stem cells are undifferentiated elements capable to acquire specific phenotype under different kind of stimuli, being a main tool for regenerative medicine. Within this context, we have previously shown that stem cells isolated from Wharton jelly multipotent stem cells (WJ-MSCs) exhibit gender differences in the expression of the stemness related gene OCT4 and the epigenetic modulator gene DNA-Methyltransferase (DNMT1). Here, we further analyze this gender difference, evaluating adipogenic and osteogenic differentiation potential, autophagic process, and expression of miR-145, miR-148a, and miR-185 in WJ-MSCs derived from males and females. These miRNAs were selected since they are involved in OCT4 and DNMT1 gene expression, and in stem cell differentiation. Our results indicate a difference in the regulatory circuit involving miR-148a/DNMT1/OCT4 autophagy in male WJ-MSCs as compared to female cells. Moreover, no difference was detected in the expression of the two-differentiation regulating miRNA (miR-145 and miR-185). Taken together, our results highlight a different behavior of WJ-MSCs from males and females, disclosing the chance to better understand cellular processes as autophagy and stemness, usable for future clinical applications

A regulated deficit irrigation strategy for hedgerow olive orchards with high plant density

Background & Aims There is not a consensus on the best irrigation approach for super-high density (SHD) olive orchards. Our aim was to design and test a regulated deficit irrigation (RDI) strategy for a sustainable balance between water saving, tree vigour and oil production. Methods We tested our RDI strategy for 3 years in an ‘Arbequina’ orchard with 1,667 trees ha−1. Two levels of irrigation reduction were applied, 60RDI and 30RDI, scaled to replacing 60 % and 30 %, respectively, of the of irrigation needs (IN). We also had a full irrigation (FI) treatment as control, with IN totalling 4,701 m3 ha−1 Results The 30RDI treatment showed the best balance between water saving, tree vigour and oil production. With a yearly irrigation amount (IA) of 1,366 m3 ha−1, which meant 72 % water saving as compared to FI, the reduction in oil yield was 26 % only. Conclusions Our results, together with recent knowledge on the effect of water stress on fruit development, allowed us to suggest a potentially improved RDI strategy for which a total IA of ca. 2,100 m3 ha−1 was calculated. Both some management details and the benefits of this suggested RDI strategy are still to be tested

Decay in survival motor neuron and plastin 3 levels during differentiation of iPSC-derived human motor neurons

Spinal muscular atrophy (SMA) is a neuromuscular disease caused by mutations in Survival Motor Neuron 1 (SMN1), leading to degeneration of alpha motor neurons (MNs) but also affecting other cell types. Induced pluripotent stem cell (iPSC)-derived human MN models from severe SMA patients have shown relevant phenotypes. We have produced and fully characterized iPSCs from members of a discordant consanguineous family with chronic SMA. We differentiated the iPSC clones into ISL-1+/ChAT+ MNs and performed a comparative study during the differentiation process, observing significant differences in neurite length and number between family members. Analyses of samples from wild-type, severe SMA type I and the type IIIa/IV family showed a progressive decay in SMN protein levels during iPSC-MN differentiation, recapitulating previous observations in developmental studies. PLS3 underwent parallel reductions at both the transcriptional and translational levels. The underlying, progressive developmental decay in SMN and PLS3 levels may lead to the increased vulnerability of MNs in SMA disease. Measurements of SMN and PLS3 transcript and protein levels in iPSC-derived MNs show limited value as SMA biomarkers

CATMoS: Collaborative Acute Toxicity Modeling Suite.

BACKGROUND: Humans are exposed to tens of thousands of chemical substances that need to be assessed for their potential toxicity. Acute systemic toxicity testing serves as the basis for regulatory hazard classification, labeling, and risk management. However, it is cost- and time-prohibitive to evaluate all new and existing chemicals using traditional rodent acute toxicity tests. In silico models built using existing data facilitate rapid acute toxicity predictions without using animals. OBJECTIVES: The U.S. Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) Acute Toxicity Workgroup organized an international collaboration to develop in silico models for predicting acute oral toxicity based on five different end points: Lethal Dose 50 (LD50 value, U.S. Environmental Protection Agency hazard (four) categories, Globally Harmonized System for Classification and Labeling hazard (five) categories, very toxic chemicals [LD50 (LD50≤50mg/kg)], and nontoxic chemicals (LD50>2,000mg/kg). METHODS: An acute oral toxicity data inventory for 11,992 chemicals was compiled, split into training and evaluation sets, and made available to 35 participating international research groups that submitted a total of 139 predictive models. Predictions that fell within the applicability domains of the submitted models were evaluated using external validation sets. These were then combined into consensus models to leverage strengths of individual approaches. RESULTS: The resulting consensus predictions, which leverage the collective strengths of each individual model, form the Collaborative Acute Toxicity Modeling Suite (CATMoS). CATMoS demonstrated high performance in terms of accuracy and robustness when compared with in vivo results. DISCUSSION: CATMoS is being evaluated by regulatory agencies for its utility and applicability as a potential replacement for in vivo rat acute oral toxicity studies. CATMoS predictions for more than 800,000 chemicals have been made available via the National Toxicology Program's Integrated Chemical Environment tools and data sets (ice.ntp.niehs.nih.gov). The models are also implemented in a free, standalone, open-source tool, OPERA, which allows predictions of new and untested chemicals to be made. https://doi.org/10.1289/EHP8495

Vowel reduction in word-final position by early and late Spanish-English bilinguals

Vowel reduction is a prominent feature of American English, as well as other stress-timed languages. As a phonological process, vowel reduction neutralizes multiple vowel quality contrasts in unstressed syllables. For bilinguals whose native language is not characterized by large spectral and durational differences between tonic and atonic vowels, systematically reducing unstressed vowels to the central vowel space can be problematic. Failure to maintain this pattern of stressed-unstressed syllables in American English is one key element that contributes to a ?foreign accent? in second language speakers. Reduced vowels, or ?schwas,? have also been identified as particularly vulnerable to the co-articulatory effects of adjacent consonants. The current study examined the effects of adjacent sounds on the spectral and temporal qualities of schwa in word-final position. Three groups of English-speaking adults were tested: Miami-based monolingual English speakers, early Spanish-English bilinguals, and late Spanish-English bilinguals. Subjects performed a reading task to examine their schwa productions in fluent speech when schwas were preceded by consonants from various points of articulation. Results indicated that monolingual English and late Spanish-English bilingual groups produced targeted vowel qualities for schwa, whereas early Spanish-English bilinguals lacked homogeneity in their vowel productions. This extends prior claims that schwa is targetless for F2 position for native speakers to highly-proficient bilingual speakers. Though spectral qualities lacked homogeneity for early Spanish-English bilinguals, early bilinguals produced schwas with near native-like vowel duration. In contrast, late bilinguals produced schwas with significantly longer durations than English monolinguals or early Spanish-English bilinguals. Our results suggest that the temporal properties of a language are better integrated into second language phonologies than spectral qualities. Finally, we examined the role of nonstructural variables (e.g. linguistic history measures) in predicting native-like vowel duration. These factors included: Age of L2 learning, amount of L1 use, and self-reported bilingual dominance. Our results suggested that different sociolinguistic factors predicted native-like reduced vowel duration than predicted native-like vowel qualities across multiple phonetic environments