Secondary Sclerosing Cholangitis in Critically Ill Patients Alters the Gut–Liver Axis: A Case Control Study

Secondary sclerosing cholangitis in critically ill patients (SC-CIP) occurs after long-term intensive care treatment. This study aimed to assess the gut–liver axis in SC-CIP. Stool microbiome composition, gut permeability, bacterial translocation and serum bile acid profiles of 18 SC-CIP patients compared to 11 patients after critical illness without liver disease (CIP controls), 21 patients with cirrhosis and 21 healthy controls were studied. 16S rDNA was isolated from stool and sequenced using the Illumina technique. Diamine oxidase, zonulin, soluble CD14 (sCD14) and lipopolysaccharide binding protein were measured in serum and calprotectin in stool. Serum bile acids were analyzed by high-performance liquid chromatography-mass spectrometry (HPLC-MS). Reduced microbiome alpha diversity and altered beta diversity were seen in SC-CIP, CIP controls and cirrhosis compared to healthy controls. SC-CIP patients showed a shift towards pathogenic taxa and an oralization. SC-CIP, CIP controls and cirrhotic patients presented with impaired gut permeability, and biomarkers of bacterial translocation were increased in SC-CIP and cirrhosis. Total serum bile acids were elevated in SC-CIP and cirrhosis and the bile acid profile was altered in SC-CIP, CIP controls and cirrhosis. In conclusions, observed alterations of the gut–liver axis in SC-CIP cannot solely be attributed to liver disease, but may also be secondary to long-term intensive care treatment

The Sitan-countries: History as Challenge Along the Silk Road

The Eurasian trade route was more than 6,000 kilometers long and split into several sub-strands and has a long history. Now all of these routes have been revived. With a corresponding expansion of the infrastructure, certain circumstances are still challenging in the 21st century: nationalism, especially among the former Soviet states, security in times of global terror, and economic competition. The Central Asian region is a melting pot of different ethnic groups, in ancient, as well as more recent, history, sometimes with more harmonious coexistence, and sometimes with more conflicts that influence politics and social background. The author traveled through the individual states, examined the ecological-economic factors and conducted interviews to clarify socio-political views. This paper examines the implications regarding economy and politics, the cultural background, and to where it refers

AN AEGEAN MIRROR FROM HALA SULTAN TEKKE, CYPRUS

Publication status: PublishedSummaryThis study deals with the results of the 2023 fieldwork at the extramural cemetery of the Late Bronze Age harbour city of Hala Sultan Tekke. One of the three excavated tombs in 2023 was the undisturbed Chamber Tomb XX, which is dated around 1300 BC. It contained a riveted bronze mirror, a rare type in Cyprus at that time, which is part of a mortuary context of four individuals out of a total of 17 individuals and 264 complete objects, many of them imported from a vast area, which includes the Mycenaean, Minoan, Egyptian and Levantine cultures. The current paper presents this mirror and associated contexts. As regards the provenance of the mirror, the Aegean is suggested as the area of manufacture, and more precisely Crete, suggesting potential evidence of direct contact between individuals from Crete and Hala Sultan Tekke.</jats:p

A single alcohol binge impacts on neutrophil function without changes in gut barrier function and gut microbiome composition in healthy volunteers.

Alcohol binge drinking is a dangerous drinking habit, associated with neurological problems and inflammation. The impact of a single alcohol binge on innate immunity, gut barrier and gut microbiome was studied. In this cohort study 15 healthy volunteers received 2 ml vodka 40% v/v ethanol/kg body weight. Neutrophil function was studied by flow cytometry; markers of gut permeability and inflammation (lactulose/mannitol/sucrose test, zonulin, calprotectin, diamino-oxidase) were studied with NMR spectroscopy and enzyme-linked immunosorbent assay in urine, stool and serum respectively. Bacterial products in serum were quantified using different reporter cell lines. Gut microbiome composition was studied by 16S rDNA sequencing and bioinformatics analysis. After a single alcohol binge, neutrophils were transiently primed and the response to E.coli stimulation with reactive oxygen species (ROS) production was transiently increased, on the other hand the percentage of neutrophils that did not perform phagocytosis increased. No changes in gut permeability, inflammatory biomarker, bacterial translocation and microbiome composition could be detected up to 4 hours after a single alcohol binge or on the next day. A single alcohol binge in young, healthy volunteers transiently impacts on neutrophil function. Although the exact biological consequence of this finding is not clear yet, we believe that this strengthens the importance to avoid any alcohol binge drinking, even in young, otherwise healthy persons