Bat guano and historical evidence of climate changes in the west of Iran during the Late Holocene (Meghalayan Stage)

An 86 cm thick sequence of bat guano layers in the Kolatarika Cave in Kurdistan province in the west of Iran was analysed. The sequence was radiocarbon dated and covers an age of approximately 4060 years. The results of geochemical data, statistical studies, along with the investigation, analysis and explanation of historical sources indicate the presence of warm and dry climate conditions between ca 2100 BC and 800 CE. These were contemporaneous with the occurrence of periods of drought and famine during the Achaemenid and Sassanid empires, and might have been was one of the causes of their ‘collapse. The existence of humid climate conditions between 800 and 1450 AD was contemporaneous with the period of Medieval Climate Anomaly and the historically documented prosperity of farms and agriculture during the Seljuk dynasties, the Samanids, and the rise of rainfall and river floods during the period of the Abbasid caliphate. The presence of cold and humid climate conditions between ca 1600 and 1750 AD was consistent with the so-called Little Ice Age and the Maunder Minimum. After this period, the climate of this area changed to warm and dry which was contemporaneous with the occurrence of famine and subsequent droughts of the late Safavid and Qajar dynasties in Iran.Key words: palaeoclimate, geochemistry, Little Ice Age, Medieval Climate Anomaly, Kolatarika Cave, Iran.Poznoholocenske podnebne spremembe v zahodnem Iranu: analize netopirskega gvana in zgodovinskih dokumentovV članku predstavimo analize 86 centimetrov debelega zaporedja plasti netopirskega gvana v jami Kolatarika v provinci Kurdistan, Zahodni Iran. Radiometrično ugotovljen starostni razpon gvana obsega zadnjih 4060 let. Statistična obravnava geokemičnih analiz gvana in raziskava zgodovinskih virov kažeta na toplo in suho podnebje v obdobju 2100 pr. n. št. in 800 n. št. To sovpada s sušami in lakoto v času ahamenidskega in sasanidskega cesarstva, kar je bil najverjetneje tudi vzrok njunega propada. Analize gvana kažejo na vlažno podnebje med 800 n. št. in 1450 n. št., kar ustreza srednjeveški podnebni anomaliji in obdobju razvoja kmetijstva in blaginje v času dinastij Seldžukov in Samanidov. Vlažno podnebje s padavinami je povzročilo tudi poplavljanje rek v času abasidskega kalifata. Hladno in vlažno obdobje med letoma 1600 in 1700 sovpada z malo ledeno dobo oziroma Maunderjevim minimumom. Po tem obdobju podnebje postane toplejše in bolj suho, kar spet sovpada z lakoto in sušami v poznem obdobju safavidske in kadžarske dinastije v Iranu.Ključne besede: paleoklima, geokemija, mala ledena doba, srednjeveška podnebna anomalija, jama Kolatarika, Iran.

Influence of lipopolysaccharide on proinflammatory gene expression in human corneal, conjunctival and meibomian gland epithelial cells

PURPOSE: Lipopolysaccharide (LPS), a bacterial endotoxin, is known to stimulate leuokotriene B4 (LTB4) secretion by human corneal (HCECs), conjunctival (HConjECs) and meibomian gland (HMGECs) epithelial cells. We hypothesize that this LTB4 effect represents an overall induction of proinflammatory gene expression in these cells. Our objective was to test this hypothesis. METHODS: Immortalized HCECs, HConjECs and HMGECs were cultured in the presence or absence of LPS (15 μg/ml) and ligand binding protein (LBP; 150 ng/ml). Cells were then processed for RNA isolation and the analysis of gene expression by using Illumina BeadChips, background subtraction, cubic spline normalization and GeneSifter software. RESULTS: Our findings show that LPS induces a striking increase in proinflammatory gene expression in HCECs and HConjECs. These cellular reactions are associated with a significant up-regulation of genes associated with inflammatory and immune responses (e.g. IL-1β, IL-8, and tumor necrosis factor), including those related to chemokine and Toll-like receptor signaling pathways, cytokine-cytokine receptor interactions, and chemotaxis. In contrast, with the exception of Toll-like signaling and associated innate immunity pathways, almost no proinflammatory ontologies were upregulated by LPS in HMGECs. CONCLUSIONS: Our results support our hypothesis that LPS stimulates proinflammatory gene expression in HCECs and HConjECs. However, our findings also show that LPS does not elicit such proinflammatory responses in HMGECs

Case Series: Cancer-Related Facial Pain Treated with Stellate Ganglion Block

Stellate ganglion block (SGB) is believed to modify the pathologic sympathetic pain response and has been commonly used to treat complex regional pain syndrome. We report successful treatment of cancer-related facial pain with SGB in three patients, suggesting a possible sympathetic pain-related mechanism. All patients exhibited clinically significant improvement of pain 12 weeks following the procedure. SGB should be considered a palliative pain treatment option in cancer-related facial pain

Testosterone Influence on Gene Expression in Lacrimal Glands of Mouse Models of Sjögren Syndrome

Purpose: Sjögren syndrome is an autoimmune disorder that occurs almost exclusively in women and is associated with extensive inflammation in lacrimal tissue, an immune-mediated destruction and/or dysfunction of glandular epithelial cells, and a significant decrease in aqueous tear secretion. We discovered that androgens suppress the inflammation in, and enhance the function of, lacrimal glands in female mouse models (e.g., MRL/MpJ-Tnfrsf6lpr [MRL/lpr]) of Sjögren syndrome. In contrast, others have reported that androgens induce an anomalous immunopathology in lacrimal glands of nonobese diabetic/LtJ (NOD) mice. We tested our hypothesis that these hormone actions reflect unique, strain- and tissue-specific effects, which involve significant changes in the expression of immune-related glandular genes. Methods: Lacrimal glands were obtained from age-matched, adult, female MRL/lpr and NOD mice after treatment with vehicle or testosterone for up to 3 weeks. Tissues were processed for analysis of differentially expressed mRNAs using CodeLink Bioarrays and Affymetrix GeneChips. Data were analyzed with bioinformatics and statistical software. Results: Testosterone significantly influenced the expression of numerous immune-related genes, ontologies, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in lacrimal glands of MRL/lpr and NOD mice. The nature of this hormone-induced immune response was dependent upon the autoimmune strain, and was not duplicated within lacrimal tissues of nonautoimmune BALB/c mice. The majority of immune-response genes regulated by testosterone were of the inflammatory type. Conclusions: Our findings support our hypothesis and indicate a major role for the lacrimal gland microenvironment in mediating androgen effects on immune gene expression

Quantum teleportation with nonclassical correlated states in noninertial frames

Quantum teleportation is studied in noninertial frame, for fermionic case, when Alice and Bob share a general nonclassical correlated state. In noninertial frames two fidelities of teleportation are given. It is found that the average fidelity of teleportation from a separable and nonclassical correlated state is increasing with the amount of nonclassical correlation of the state. However, for any particular nonclassical correlated state, the fidelity of teleportation decreases by increasing the acceleration.Comment: 10 pages, 3 figures, expanded version to appear in Quantum Inf. Proces

Dihydrotestosterone suppression of proinflammatory gene expression in human meibomian gland epithelial cells

Purpose: We discovered that dihydrotestosterone (DHT) decreases the ability of lipopolysaccharide, a bacterial toxin, to stimulate the secretion of leukotriene B4, a potent proinflammatory mediator, by immortalized human meibomian gland epithelial cells (IHMGECs). We hypothesize that this hormone action reflects an androgen suppression of proinflammatory gene activity in these cells. Our goal was to test this hypothesis. For comparison, we also examined whether DHT treatment elicits the same effect in immortalized human corneal (IHC) and conjunctival (IHConj) ECs. Methods: Differentiated cells were cultured in media containing vehicle or 10 nM DHT. Cells (n = 3 wells/treatment group) were then processed for RNA isolation and the analysis of gene expression by using Illumina BeadChips, background subtraction, cubic spline normalization and Geospiza software. Results: Our results demonstrate that DHT significantly suppressed the expression of numerous immune-related genes in HMGECs, such as those associated with antigen processing and presentation, innate and adaptive immune responses, chemotaxis, and cytokine production. DHT also enhanced the expression of genes for defensin β1, IL-1 receptor antagonist, and the anti-inflammatory serine peptidase inhibitor, Kazal type 5. In contrast, DHT had no effect on proinflammatory gene expression in HCECs, and significantly increased 33 gene ontologies linked to the immune system in HConjECs. Conclusions: Our findings support our hypothesis that androgens suppress proinflammatory gene expression in IHMGECs. This hormone effect may contribute to the typical absence of inflammation within the human meibomian gland

Corneal Epithelial Immune Dendritic Cell Alterations in Subtypes of Dry Eye Disease: A Pilot In Vivo Confocal Microscopic Study

Citation: Kheirkhah A, Rahimi Darabad R, Cruzat A, et al. Corneal epithelial immune dendritic cell alterations in subtypes of dry eye disease: a pilot in vivo confocal microscopic study. Invest Ophthalmol Vis Sci. 2015;56:7179-7185. DOI:10.1167/ iovs.15-17433 PURPOSE. To evaluate density and morphology of corneal epithelial immune dendritic cells (DCs) in different subtypes of dry eye disease (DED) using in vivo confocal microscopy (IVCM). METHODS. This retrospective study included 59 eyes of 37 patients with DED and 40 eyes of 20 age-matched healthy controls. Based on clinical tests, eyes with DED were categorized into two subtypes: aqueous-deficient (n ¼ 35) and evaporative (n ¼ 24). For all subjects, images of laser scanning in vivo confocal microscopy (IVCM) of the central cornea were analyzed for DC density and DC morphology (DC size, number of dendrites, and DC field). These DC parameters were compared among all dry eye and control groups. RESULTS. Compared with the controls, patients with DED had significantly higher DC density, larger DC size, higher number of dendrites, and larger DC field (all P < 0.001). Comparison between aqueous-deficient and evaporative subtypes demonstrated that DC density was significantly higher in aqueous-deficient subtype (189.8 6 36.9 vs. 58.9 6 9.4 cells/mm 2 , P ¼ 0.001). However, there were no significant differences in morphologic parameters between DED subtypes. When aqueous-deficient DED with underlying systemic immune disease (Sjögren's syndrome and graft versus host disease) were compared with nonimmune conditions, the immunologic subgroup showed significantly higher DC density, DC size, and number of dendrites (all P < 0.05). CONCLUSIONS. Corneal IVCM demonstrated differential changes in DC density and morphologic DC parameters between subtypes of DED. These changes, which reflect the degree of immune activation and inflammation in DED, can be used for clinical practice and endpoints in clinical trials. Keywords: dry eye disease, in vivo confocal microscopy, inflammation, dendritic cells D ry eye disease (DED) is one of the most commonly encountered ophthalmic disorders. It is a multifactorial disease of the ocular surface and tear film, characterized by symptoms of eye irritation, tear instability, and vision impairment. 1 In addition to evaluating symptoms, a variety of clinical tests are currently being used to diagnose DED, including the Schirmer's wetting test, tear break-up time (TBUT), tear osmolarity, and vital dye staining of the ocular surface by fluorescein, Rose Bengal and Lissamine Green. However, complex clinical features of the disease make the diagnosis a challenge in many cases. 2,3 Therefore, there remains a significant need for objective tests, which can be used to accurately diagnose DED and/or monitor therapeutic response in DED and its underlying changes. Recent studies have shown that the immune changes play an important role in the pathogenesis of DED. To evaluate changes in DCs in patients with DED, corneal in vivo confocal microscopy (IVCM) has lately been used. In vivo confocal microscopy is a noninvasive imaging modality that enables studying the cornea at a cellular level

Overgeneral Memory Retrieval an Ineffective Problem-Solving in Depressed Patients with Suicidal Ideation: implications for Therapy

The present study was designed to extend our knowledge around cognitive aspects of memory retrieval and problem-solving in two groups of clinically depressed patients. We administered an autobiographical memory test, a means-ends problem-solving task, Beck Depression Inventory, and Beck Hopelessness Scale to measure respectively level of specificity in memory retrieval, problem-solving effectiveness, depression and hopelessness in clinically depressed patients with suicidal ideation and in a matched control group of depressed patients without suicide ideation. Both groups met DSM-IV criteria for major depression disorder without psychotic features. The depressed suicide-ideators were significantly more hopeless than their non-ideator counterparts. Also an overall tendency was found for the ideator group to retrieve less specific memories compared to the non-ideator group. Depressed patients’ bias toward negative memory retrieval is broadly documented. Access to non-specific memories in depressed people can lead to ineffective problem solutions and subsequently hopelessness. By this account, this very vicious cycle continues over time to resonate powerfully, perhaps leading to suicidal attempt. An emergence of suicidal ideation when assessing helps clinicians identify those who are most at risk for suicide. They can be targeted then for intensive therapy designed to lower the risk.El presente estudio se diseñó con el propósito de ampliar el conocimiento acerca de los aspectos cognifivos de la recuperación de memoria y la solución de problemas en dos grupos de pacientes con depresión: pacientes con ideación suicida y pacientes sin ideación suicida. Ambos grupos cumplían los criterios DSM-IV para el trastorno de depresión mayor sin características psicóticas. Se administraron a los participantes una prueba de memoria autobiográfica, una tarea de solución de problemas, el Inventario de Depresión y la Escala de Desesperanza de Beck, para medir el nivel de especificidad en la recuperación de recuerdos, la efectividad en solución de problemas, depression and hopelessness. Los ideadores suicidas puntuaron significativamente más alto en desesperanza que los no ideadores. Se encontró una tendencia global en el grupo de ideadores a recuperar menos memorias específicas en comparación con los no ideadores. Se discuten los resultados de acuerdo al conocido sesgo en la recuperación de memorias negativas en pacientrs depresivos y la emergencia de la ideación suicida como identificador de riesgo para el suicidio.