The invariant manifold approach applied to nonlinear dynamics of a rotor-bearing system

The invariant manifold approach is used to explore the dynamics of a nonlinear rotor, by determining the nonlinear normal modes, constructing a reduced order model and evaluating its performance in the case of response to an initial condition. The procedure to determine the approximation of the invariant manifolds is discussed and a strategy to retain the speed dependent effects on the manifolds without solving the eigenvalue problem for each spin speed is presented. The performance of the reduced system is analysed in function of the spin speed

Management of phosphorus supply to Australian floricultural species

Young plants of Sticherus, waxflower (Chamelaucium) and two Caustis cultivars were grown in a soilless potting medium. The plants were fertilised at seven rates of the soluble monocalcium phosphate (MCP) fertiliser and one rate of the slightly soluble rock phosphate (RP). One group of plants was watered by a non-leaching and a second group of plants watered by a leaching method. Both Caustis cultivars (M63, B84) grown in the potting medium under the non-leaching regime did not show deficiency symptoms at no added P, but they showed P toxicity symptoms at MCP-P application rates > 11 g m-3. The critical P concentration in the shoots associated with a 10% decrease in yield from the maximum was 0.26% for Caustis M63 and 0.33% for Caustis B84. The two Caustis cultivars did not develop P toxicity symptoms or show any decline in shoot dry weight when fertilised with RP under both non-leaching and leaching regimes. Leaching reduced but did not eliminate P toxicity in both Caustis cultivars, although shoot dry weight did not differ significantly between the two watering regimes used. Waxflower grew poorly in the potting medium under the non-leaching regime at no added P or when fertilised with RP but did not develop P toxicity symptoms at any rate of P application as MCP, including the highest rate of 352 g m-3. The waxflower plants grew less well under the leaching than when grown under the non-leaching regime. The critical NaHCO3-extractable P level required for production of 90% relative dry weight of shoots of waxflower was about 46 mg kg-1 under the non-leaching and about 69 mg kg-1 medium under the leaching regime. Under the non-leaching regime the critical NaHCO3-extractable P level for toxicity of Caustis B84 was about 13 mg kg-1 while under the leaching regime this was higher at about 22 mg kg-1. Sticherus plants did not respond significantly to P application as either MCP or RP. However, Sticherus plants grew much better when grown under the leaching than when they were grown under the non-leaching regime. Although leaching conferred some advantages in the growth of Sticherus and in the reduction of P toxicity in Caustis, it also caused a considerable loss of P

Genome-Wide Profiling of Pluripotent Cells Reveals a Unique Molecular Signature of Human Embryonic Germ Cells

Human embryonic germ cells (EGCs) provide a powerful model for identifying molecules involved in the pluripotent state when compared to their progenitors, primordial germ cells (PGCs), and other pluripotent stem cells. Microarray and Principal Component Analysis (PCA) reveals for the first time that human EGCs possess a transcription profile distinct from PGCs and other pluripotent stem cells. Validation with qRT-PCR confirms that human EGCs and PGCs express many pluripotency-associated genes but with quantifiable differences compared to pluripotent embryonic stem cells (ESCs), induced pluripotent stem cells (IPSCs), and embryonal carcinoma cells (ECCs). Analyses also identified a number of target genes that may be potentially associated with their unique pluripotent states. These include IPO7, MED7, RBM26, HSPD1, and KRAS which were upregulated in EGCs along with other pluripotent stem cells when compared to PGCs. Other potential target genes were also found which may contribute toward a primed ESC-like state. These genes were exclusively up-regulated in ESCs, IPSCs and ECCs including PARP1, CCNE1, CDK6, AURKA, MAD2L1, CCNG1, and CCNB1 which are involved in cell cycle regulation, cellular metabolism and DNA repair and replication. Gene classification analysis also confirmed that the distinguishing feature of EGCs compared to ESCs, ECCs, and IPSCs lies primarily in their genetic contribution to cellular metabolism, cell cycle, and cell adhesion. In contrast, several genes were found upregulated in PGCs which may help distinguish their unipotent state including HBA1, DMRT1, SPANXA1, and EHD2. Together, these findings provide the first glimpse into a unique genomic signature of human germ cells and pluripotent stem cells and provide genes potentially involved in defining different states of germ-line pluripotency

Compositional Analysis of Carbohydrates of a Family of Legumes

Legumes, most commonly identified as beans or lentils, provide a good source of both protein and carbohydrates. Many legumes contain the polysaccharide arabinogalactans, classified as dietary fiber and have unique functional properties in foods. However, these, and other plant polysaccharides have not been well characterized. A preliminary collaborative study between Florida State University and the Whistler Center at Purdue indicated that isolated legume arabinogalactans appear to have high texturizing capability through formation of viscoelastic structures. Their soluble property may also present unique fiber nutritional trait. These properties of arabinogalactans and their variability among different legumes are yet to be analyzed. In this study the carbohydrate composition of the 24 most common legumes were analyzed. Gas chromatography (GC) is routinely used to determine the carbohydrate, or monosaccharide, composition by hydrolyzing and converting sugars into volatile derivatives. Results from the experiment revealed that the legumes varied in their abundance of both arabinose and galactose. Legumes with higher relative abundance of these polysaccharides have higher dietary fiber content. These results provide a better understanding of carbohydrate composition of legumes including arabinogalactans. In the remainder of the project, variability in polysaccharide contents and ratios of arabinose/galactose will be assessed to obtain a comprehensive picture of the structural differences among the arabinogalactans of the 20 legume varieties. In the future, continuing with linkage analysis of arabinogalactans will enable us understand detailed structure of the polysaccharides and identify chemical and genetic markers for breeding of high quality legumes or development of new bean varieties