RAGHOTHAMA, K. G.

VASCONCELOS, M. J. V. de

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  Plant & Animal Genomes XV ConferenceJanuary 13-17, 2007Town & Country Convention CenterSan Diego, CAP747 : MicroarraysExpression Of Bat-3 And Phosphate Stress Induced Genes In Maize And SorghumMaria Jose V Vasconcelos1 , Kashchandra G Raghothama21  Rodovia MG 424 Km 65, P.O.Box 151, Sete Lagoas-MG, 35701-970, Brazil2  1165 Agriculture Mall, Department of Horticulture and Landscape Architecture, Purdue University, WestLafayette-IN 47907, USAPhosphate unavailability is a growth-limiting factor for plants in many natural ecosystems. To thrive under Pilimited conditions plants have developed efficient mechanisms of phosphate acquisition and utilization of absorbedphosphorus in plant tissue. In order to decipher molecular determinants which could have a potential role inphosphorus acquisition and utilization efficiency, further studies on expression profiles of some of the phosphateresponsive genes identified through microarray analysis were done. The advent of DNA microarray technology hasmade possible the analysis of global patterns of gene expression and revealed unexpected networks ofcoordinated regulation (Lockhart et al., 1996). The evaluation of expression of thousands of genes by microarrayanalyses in parallel has become an important tool in functional genomics. Recently, Girke et al. (2000) haveidentified genes which are transcriptionally regulated by different concentrations of phosphate. In collaboration withPioneer HiBred Co. we have conducted a microarray analysis of a subset of genes expressed in maize. Themicroarray chips used were referred to as metabolic chips and transcription factor chips. Analysis was done withRNA isolated from P+ and P- roots and cobs. A set of genes were selected for expression analysis and furthercharacterization. Based on the results of RNA blot experiments, Bat-3 gene was selected for further maize andsorghum analyses described in this study.PAG-XV (P747) Expression Of Bat-3 And Phosphate Stress Induced G... http://www.intl-pag.org/15/abstracts/PAG15_P07a_747.html1 de 1 23/5/2011 15:44

Expression of Bat-3 and phosphate stress induced genes In maize and Sorghum.

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Expression of Bat-3 and phosphate stress induced genes In maize and Sorghum.

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