A fixed combination of probiotics and herbal extracts attenuates intestinal barrier dysfunction from inflammatory stress in an in vitro model using Caco-2 cells.

Background: Inflammatory Bowel Diseases (IBD), are considered a growing global disease, with about ten million people being affected worldwide. Maintenance of intestinal barrier integrity is crucial for preventing IBD onset and exacerbations. Some recent patents regarding oily formulations containing probiotics (WO2010122107A1 and WO2010103374A9) and the use of probiotics for gastrointestinal complaints (US20110110905A1 and US9057112B2) exist, or are pending application. Objective: In this work, we studied the effect of a fixed combination of registered Lactobacillus reuteri and Lactobacillus acidophilus strains and herbal extracts in an in vitro inflammation experimental model. Methods: Caco-2 cell monolayer was exposed to INF-\u3b3+TNF-\u3b1 or to LPS; Trans Epithelial Electrical Resistance (TEER) and paracellular permeability were investigated. ZO-1 and occludin Tight Junctions (TJs) were also investigated by mean of immunofluorescence. Results: Pre-treatment with the fixed combination of probiotics and herbal extracts prevented the inflammation-induced TEER decrease, paracellular permeability increase and TJs translocation. Conclusions: In summary, the fixed combination of probiotics and herbal extracts investigated in this research was found to be an interesting candidate for targeting the re-establishment of intestinal barrier function in IBD conditions

Tamanho mínimo de amostra de plantas e frutos do algodoeiro para monitoramento das populações de Anthonomus grandis Boh. (Coleoptera: Curculionidae).

Dois modelos estatísticos foram desenvolvidos visando determinar o tamanho mínimo de amostra para monitoramento de populações do bicudo do algodoeiro, Anthonomus grandis Boh. (Coleoptera: Curculionidae). A amostra consistiu no número mínimo de plantas e/ou frutos do algodoeiro avaliados para se detectar as densidades populacionais do inseto próximas ao limiar econômico, considerando-se ainda a área da cultura a ser monitorada

Poultry manure gasification and its energy yield

The disposal of poultry manure is one of the main problems of the poultry sector. The results of some tests developed in a gasification pilot plant are presented in this paper. The aim was to preliminarily analyze the energy generation from poultry manure treated by gasification. The level of energy yields and the quantification of the produced syngas are also reported. The good results suggests an possible application at full scale

Perspectives of decentralised gasification of residual municipal solid waste

In the European Union (EU), the dominant technology for high-temperature thermal treatment of residual Municipal Solid Waste (i.e., the unsorted waste where source separation is performed) is the moving grate incineration. The process of combustion in this sector has been optimised thanks to the introduction of stringent criteria of operation both in the combustion chamber and in the treatment of the generated off-gas. However, the costs of treatment can be sustainable only if the tariff to be applied for the service is coherent with the average value found in the sector. The literature demonstrates that, under a capacity threshold, the grate system is out of market, thus limiting the implementation of small decentralised plants. This paper discusses the potential benefits of small-scale and decentralised thermo-chemical treatment plants replacing a single large-scale one. In addition, the present article analyses the consequences of the results of a recent survey that zoomed in on the availability of small-scale gasifiers for implementing such a strategy. The results of this analysis show that small-scale gasification is preferable to other technologies (e.g., incineration and pyrolysis) in terms of scale effect and flexibility/modularity. Compared to other thermal treatments, the local environmental impact could be reduced by converting syngas into fuels or chemicals rather than burning it for direct energy recovery. The paper also shows that small-scale gasification is able to respond to different needs in both EU and non-EU countries, like the management of progressively lower amounts of residual waste requiring treatment/management of uncontrolled dump sites

Critical Analysis of the GreenMetric World University Ranking System: The Issue of Comparability

The Universitas Indonesia GreenMetric World Ranking is the most widely adopted system nowadays to rank worldwide universities' sustainability. The number of participating universities has consistently increased throughout the last decade. An in-depth analysis of this ranking system is made to assess how sustainability in universities is measured through specific indicators. Specifically, based on expert knowledge, common logic and the scientific literature, these indicators are assessed with respect to whether they can be used to fairly quantify and rank worldwide universities' sustainability development. Some indicators proposed by the ranking system, such as the number of renewable energy sources on campus and the number of various types of programs for sustainable development, were found to be unable to measure any sustainability development effectively and fairly. Many others, such as the opted sewage disposal modality, the percentage of university budget for sustainability efforts and the ratio of sustainability research funding to total research funding, were found to need adjustment to account for context-specific factors such as availability of renewable energy sources, weather, landscape, original construction and the cultural habits of the enrolled people. Taking into account these considerations, a fairer evaluation and comparison of universities' sustainability could be achieved which provides universities with information on how to effectively improve their sustainability

Potentials of the waste-to-energy sector for an unconventional district heating system

In spite of being a process that exploits a renewable source of energy, the combustion of wood-based biomass contributes to deteriorate outdoor and indoor air quality. Critical situations for human exposure may occur in mountainous areas, where wood-based biomass is usually abundant and the complex morphology may favour the stagnation of air pollutants in valleys. Replacing wood/pellet stoves with centralised systems would reduce the impact, but the construction of district heating systems may not be convenient in areas with low density of houses. A possible solution could rely on direct electrical heating (DEH) systems, preferably fed by thermochemical processes that help achieve environmental goals for the local community, like the reduction of waste landfilling and the valorisation of the energy content of waste. This paper aims at presenting a comparison between the impacts expected by household wood/pellet stoves and by a modern waste-to-energy (WtE) plant, in terms of emissions of air pollutants into the atmosphere, when replacing wood stoves with a DEH system fed by the electric energy generated by the WtE plant. The comparison shows that the replacement of household stoves with an equivalent DEH system would be beneficial in terms of impacts on the local air quality. Such an approach could be considered to reduce the health impacts from biomass burning in critical areas like the Alpine region

Assessment of biological kinetics in a conventional municipal WWTP by means of the oxygen uptake rate method

Pollution control of surface water bodies requires stringent checks on wastewater treatment plants performances. The satisfactory operation of biological treatment, commonly performed by means of activated sludge processes, requires a number of controlling and monitoring procedures. Suitable respirometric techniques for the determination of the kinetic parameters that regulate biological processes have been implemented in order to achieve this aim. This paper describes the results of an experimental research carried out in a conventional Italian municipal wastewater treatment plant. Particularly, the research has been finalized to both evaluate the biological process for the removal of biodegradable pollutants, such as carbonaceous substrates and ammonia nitrogen, and to collect data in order to evaluate a possible plant upgrade. Heterotrophic and autotrophic biomass kinetic parameters have been examined using respirometric techniques based on oxygen uptake measurements. The research performed makes a valuable contribution toward verifying the reliability of the values proposed in the literature for some kinetic parameters, which have been commonly used for a long time

Risk assessment in a materials recycling facility: Perspectives for reducing operational issues

Mechanical separation of light packaging waste is a useful practice for improving the quality of the recyclable waste flows and its exploitation in a frame of the circular economy. Materials Recovery Facilities can treat from 3000 to 5000 tons per year of light packaging waste. Concerning the plastic content, this is divided in four flows: PET, HDPE, other plastics, and waste rejects. The last two are generally used for energy recovery. For improving the quality of the recyclable plastic waste, a manual separation is required for reducing the impurities detectable in the final products. However, this practice could enhance the risk at work of the operators, which should be constantly monitored. This article explores the main differences of a manual separation and of a mechanical separation, assessing the costs and the health risk for the workers. The analysis started from the situation in an Italian Materials Recovery Facility, generalizing the context; a future scenario with the application of a mechanical separation is theoretically introduced. The main results obtained suggest that the manual separation plant improves the quality of the material, though increasing the risk of the operators due to the possible contact with sharp waste, sanitary danger, and risk of injuries for the mismanagement of machines, among others. The mechanical separation can be considered a real advantage from an economic point of view, since the operating costs are lower and the investment could be recovered in around 10 years, in an Italian-like context. On balance, on the one hand, the article provides indications for the private sector for improving the management of a Materials Recovery Facility, while, on the other hand, it detects the main pros and cons of both methodologies. © 2018 by the authors

H-Content Is Not Predictive of Perfluorocarbon Ocular Endotamponade Cytotoxicity in Vitro

In recent years, cases of retinal toxicity occurred in some European, Middle Eastern, and South American countries following the use of perfluorocarbon liquids (PFCLs) on vitreoretinal surgeries owing to impurities in the product. Moreover, Spanish ophthalmologists reported several toxic cases on the use of perfluoro-n-octane Ala Octa (Alamedics, Dornstadt, Germany), raising the necessity of reviewing the current validated methods used for assessing the safety of PFCLs. We proved that in samples of PFCLs contaminated on purpose with impurities previously detected in Ala Octa devices, the determination of the so-called H-content using a 1H NMR quantitative assay implemented with the electronic reference to access in vivo concentrations 2 technology failed to demonstrate a correlation between the H-content and in vitro cytotoxicity test in ARPE-19 and BALB 3T3 cell lines. Therefore, direct information on the safety of PFCLs was provided only by the cytotoxicity test in vitro validated according to ISO 10993-5, and the H-content was not predictive of perfluorocarbon ocular endotamponade cytotoxicity in vitr