Are epizoites biological indicators of a western Mediterranean striped dolphin die-off?

During a die-off of Mediterranean striped dolphins Stenella coeruleoalba in 1990-91, 82 individuals stranded on the Spanish coast were examined for crustacean ectoparasites and epizoites. Six species were detected: Syncyarnus aequus, PenneUa sp., Xenobalanus globicipitis, Conchoderma virgatum, Lepas pectinata and Lepas cf. hillii. The barnacles L. pectinata and L. cf. hillii are reported here for the first time on cetaceans; they were attached to the teeth. C. virgaturn was also found on the teeth; this is an unusual attachment on cetaceans. X. globicipitis showed a higher prevalence on the dolphins studied than on those stranded in the same months before (1980 to 1990) and after (1991 to 1993) the epizootic. An analysis of shell and pseudocapitulum sizes of the X. globicipitis infrapopulations indicated possible recent colonization. It is suggested that settlement of some epizoites might have increased due to reduced movement of the dolphins and/or impaired regenerative and immune functioning of the skin prior to death. This might account for the exceptional occurrence of Lepas spp. attached on the teeth, the unusual attachment of C. virgatum, and the higher prevalence and apparently young infrapopulations of X. globicipitis.Aznar Avendano Francisco Javier [email protected] Balbuena Diaz-Pines, Juan Antonio, [email protected] ; Raga Esteve, Juan Antonio, [email protected]

A review of virus infections of cetaceans and the potential impact of morbilliviruses, poxviruses and papillomaviruses on host population dynamics

Viruses belonging to 9 families have been detected in cetaceans. We critically review the clinical features, pathology and epidemiology of the diseases they cause. Cetacean morbillivirus (family Paramyxoviridae) induces a serious disease with a high mortality rate and persists in several populations. It may have long-term effects on the dynamics of cetacean populations either as enzootic infection or recurrent epizootics. The latter presumably have the more profound impact due to removal of sexually mature individuals. Members of the family Poxviridae infect several species of odontocetes, resulting in ring and tattoo skin lesions. Although poxviruses apparently do not induce a high mortality, circumstancial evidence suggests they may be lethal in young animals lacking protective immunity, and thus may negatively affect net recruitment. Papillomaviruses (family Papovaviridae) cause genital warts in at least 3 species of cetaceans. In 10% of male Burmeister's porpoises Phocoena spinipinnis from Peru, lesions were sufficiently severe to at least hamper, if not impede, copulation. Members of the families Herpesviridae, Orthomyxoviridae and Rhabdoviridae were demonstrated in cetaceans suffering serious illnesses, but with the exception of a 'porpoise herpesvirus' their causative role is still tentative. Herpes-like viruses and caliciviruses (Caliciviridae) give rise to cutaneous diseases in Monodontidae and Delphinidae. Antibodies to several serotypes of caliciviruses were found in odontocetes and mysticetes. An unrecognized Hepadnaviridae was detected by serology in a captive Pacific white-sided dolphin Lagenorhynchus obliquidens with chronic persistent hepatitis. Adenoviruses (Adenoviridae) were isolated from the intestinal tracts of mysticeti and a beluga Delphinapterus leucas but were not associated with any pathologies. We discuss the potential impact of Paramyxoviridae, Poxviridae and Papovaviridae on the dynamics of several odontocete populations.Raga Esteve, Juan Antonio, [email protected]

Composition and structure of the parasite faunas of cod, Gadus morhua L. (Teleostei: Gadidae), in the North East Atlantic

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Reproductive inequalities in the acanthocephalan Corynosoma cetaceum: looking beyond "crowding" effects

Background: At present, much research effort has been devoted to investigate overall (average) responses of parasite populations to specific factors, e.g., density-dependence in fecundity or mortality. However, studies on parasite populations usually pay little attention to individual variation (inequality) in reproductive success. A previous study on the acanthocephalan Corynosoma cetaceum in franciscana dolphins, Pontoporia blainvillei, revealed no overall intensity-dependent, or microhabitat effects, on mass and fecundity of worms. In this study, we investigated whether the same factors could influence mass inequalities for this species of acanthocephalan.Methods: A total of 10,138 specimens of C. cetaceum were collected from 10 franciscana dolphins accidentally caught in Buenos Aires Province between 1988-1990. To investigate mass inequalities, all the specimens were sexed, and females were classified according to their developmental stage and weighted. Additionally, the relationship between biomass and fecundity (estimated as the number of acanthors) was investigated for some females. Inequalities in fecundity and biomass were assessed using standard methods, i.e. the Lorenz curve and the Gini coefficient (G).Results: We found a modest, but highly significant linear relationship between mass and fecundity. The G was very low (0.314) compared with that from other helminth species. G values were significantly lower in gravid females, which presumably exhibit a slow rate of growth. Also, G values significantly increased with total intensity, but only for gravid females, and the effect was more predictable considering only the number of gravid females.Conclusions: Apparently, competition between reproducing females increases inequality without producing crowding effects. Although the mechanism whereby this occurs is unclear, gravid females, at higher intensities, expanded their distribution and occupied gut chambers with contrasting environmental conditions, which might result in greater variability in body size. The observed inequalities are not expected to strongly influence the population genetics of C. cetaceum, but they reveal subtle individual effects beyond an overall population impact.Fil: Aznar Avendaño, Francisco Javier. Universidad de Valencia; EspañaFil: Hernández Orts, Jesús Servando. Universidad Nacional del Comahue. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni". - Provincia de Río Negro. Ministerio de Agricultura, Ganadería y Pesca. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni". Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Centro Nacional Patagónico. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni"; ArgentinaFil: Vélez Rubio, Gabriela Manuela. Universidad de Valencia; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de la República; UruguayFil: Fernández, Luis M.. Ceiba Foundation for Tropical Conservation; Estados UnidosFil: Muriel, Nadia T.. No especifíca;Fil: Raga, Juan Antonio. Universidad de Valencia; Españ

Uncinaria hamiltoni (Nematoda: Ancylostomatidae) in South American Sea Lions, Otaria flavescens, From Northern Patagonia, Argentina

Thirty-one South American sea lion pups (Otaria flavescens) found dead in Punta León, Argentina, during the summer of 2002, were examined for hookworms (Uncinaria hamiltoni). Parasite parameters were analyzed in 2 locations of the rookery, i.e., a traditional, well-structured breeding area and an expanding area with juveniles and a lax social structure. Prevalence of hookworms was 50% in both localities, and no difference was observed in prevalence between pup sexes (P > 0.05). Hookworms were concentrated in the small intestine. Transmammary transmission is assumed because only adult hookworms were found in the pups. The mean intensity of hookworms per pup was 135; the mean intensity in females (92.78) was significantly different (P 0.05) in intensity was found between the 2 breeding areas, although prevalence was higher in the traditional breeding area than in the other area. Location was the only factor affecting hookworm prevalence (P log-linear model: 0.9552; χ2: 1.5629). No apparent trend between body condition and intensity of hookworms was observed.Raga Esteve, Juan Antonio, [email protected]

Presence of Genital Spines in a Male Corynosoma cetaceum Johnston and Best, 1942 (Acanthocephala)

We collected 83 females and 80 males of Corynosoma cetaceum from 2 common dolphins, Delphinus delphis, collected in northern Patagonia (Argentina). Worms were most similar to specimens collected in other South American localities. However, 1 male had 2 spines adjacent to the genital pore and isolated from the rest of body spines. This finding confirms the recent reassignment of C. cetaceum to Corynosoma. Absence of genital spines is suggested to be avoided as the sole criterion to exclude specimens from Corynosoma or Andracantha.Aznar Avendaño, Francisco Javier, [email protected] ; Raga Esteve, Juan Antonio, [email protected]

Tagging reveals limited exchange of immature loggerhead sea turtles (Caretta caretta) between regions in the western Mediterranean

El marcaje revela un intercambio limitado de inmaduros de tortuga boba (Caretta caretta) entre regiones en el Mediterráneo occidental. – Se investigó el intercambio de inmaduros de tortuga boba (Caretta caretta) entre las regiones septentrional y meridional del Mediterráneo occidental a partir de los datos obtenidos por varios programas españoles de marcaje. El rango de la longitud recta de caparazón de las tortugas marcadas fue de 23 a 74 cm. 36 de ellas se recapturaron tras un intervalo medio de 390,5±462,6 días (DS). Como la distancia media de dispersión (MDD) de una población de tortugas que se dispersan por el Mediterráneo occidental se estabilizaría al cabo de 117 días (IC 95%: 98-149), se llevaron a cabo dos análisis incluyendo los datos de las tortugas recapturadas después de 98 y 149 días, respectivamente. En ambos análisis, la frecuencia de recaptura en la misma región donde fueron marcadas fue mayor a la esperada. En un segundo análisis se comparó la distancia media entre las localizaciones de captura y recaptura de las tortugas de cada región con la MDD esperada si permanecieran confinadas en la región de la primera captura. Se excluyeron de este análisis aquellas tortugas recapturadas en un plazo inferior a 15 días (región septentrional) y 25 días (región meridional) para garantizar la independencia entre la localidad de primera captura y de recaptura. No se hallaron diferencias significativas entre la MDD esperada y la distancia media entre las localizaciones de liberación y captura para ninguno de los dos grupos de tortugas. El conjunto de los datos disponibles demuestran la fidelidad de las tortugas inmaduras a ciertas áreas y la existencia de una fuerte barrera a la dispersión de tortugas entre las regiones septentrionales y meridionales del Mediterráneo occidental, por lo que las tortugas bobas del Mediterráneo occidental se deben dividir en al menos dos unidades de manejo.Exchange of immature loggerhead sea turtles (Caretta caretta) between the northern and southern regions of the western Mediterranean was investigated using data obtained from several Spanish tagging programmes. Tagged turtles ranged in straight carapace length from 23.0 to 74.0 cm. Thirty-six turtles were recaptured after an average interval of 390.5±462.6 days (SD). As the mean dispersal distance (MDD) of a turtle population that spreads over the western Mediterranean would stabilize after 117 days (CI 95%: 98 to 149), two analyses were conducted that included data from turtles recaptured after 98 and 149 days respectively. In both analyses, turtles were recaptured more often than expected in the same region where they had been tagged. No difference was found in either of the two regions between the average distance between the capture and recapture locations and the expected MDD if the turtles were to remain in the region where they were first captured. Turtles recaptured after 15 and 25 days respectively were excluded from the analysis to ensure data independence. The overall evidence indicates that immature turtles exhibit strong site fidelity to certain areas and that there is a strong barrier to dispersal between the northern and southern parts of the western Mediterranean. Therefore, loggerhead turtles in the western Mediterranean should be split into at least two management [email protected]

Accacoelium contortum (Trematoda : Accacoeliidae) a trematode living as a monogenean: morphological and pathological implications

Accacoelium contortum (Rudolphi, 1819) Monticelli, 1893 is a frequent but poorly known trematode found on gills, pharynx and digestive tract of the ocean sunfish Mola mola (L.). Although the morphology of A. contortum agrees with that of a typical endoparasitic trematode, with two relatively small suckers and no large holdfasts, this parasite is normally ectoparasitic. The main objective of this paper is to explore this peculiar host-parasite relationship. A total of 106 ocean sunfish were examined for the presence of A. contortum. The oropharyngeal chamber (gills and pharynx) and the digestive tract were analysed. As the previous descriptions of this species seem to be based on contracted specimens, for the morphological study the parasites were killed using two methods: with hot 70 % ethanol (with relaxed bodies) and with 70 % ethanol at room temperature (with contracted bodies). For histological studies, samples from fresh fish with parasitised left gills, pharynx and digestive tract were fixed in buffered 10 % formalin. For molecular studies the 18S, 28S and ITS-2 sequences were provided and compared with the available data in GenBank®. New information on the morphology of A. contortum and on the parasite-related response and pathological alterations in the host are given. New diagnostic traits for some structures are provided: e.g. tegumental papillae of the forebody with apical digitiform swellings and mouth surrounded by a circum-oral crown of simple papillae. The length of the ventral sucker peduncle and the position of the vitellarium were found to be associated with the contraction degree of the specimen. Immature individuals of this species are described for the first time. An intense proliferative inflammatory response of host gill and pharynx epithelium at the host-parasite interface was detected and parasites became partially covered by overgrowths of host tissues. The induction of prominent histological alterations associated with A. contortum seems to be an adaptation to the external environment, an unusual location for trematodes