o Processo Pré-transição a Aposentadoria: Desenvolvimento de Escalas Psicométricas e Correlatos Psicossociais.

RESUMO Rafalski, J. C. (2015). O processo pré-transição para a aposentadoria: desenvolvimento de escalas psicométricas e correlatos psicossociais (Dissertação de Mestrado). Programa de Pós-Graduação em Psicologia da Universidade Federal do Espírito Santo, Vitória. O envelhecimento da população e a diminuição das taxas de natalidade e fertilidade contribuíram para que a temática da aposentadoria crescesse em importância em diversas áreas como a saúde e políticas públicas, políticas organizacionais e a previdência social. Esse trabalho teve como foco compreender o processo pré-transição da aposentadoria nas suas fases de percepção, decisão e planejamento, e observar as variáveis demográficas, de saúde e trabalho que atuam de modo a influenciar essas passagens. Para tanto, a dissertação está estruturada em três artigos. O primeiro foi destinado ao desenvolvimento e validação da Escala de Percepção de Futuro da Aposentadoria (EPFA), que apresentou estrutura de cinco dimensões: Percepções de Saúde, Desligamento do Trabalho, Finanças, Relacionamentos Interpessoais e Perdas na Aposentadoria. A escala apresentou índices favoráveis de confiabilidade em todas as subscalas (acima de 0,70) e relação com outras variáveis. O segundo artigo apresenta a adaptação da Escala de Processo de Planejamento da Aposentadoria (EPPlA) do inglês para o português e a relação desta escala com estilos de tomada de decisão. A EPPlA foi adaptada satisfatoriamente e os resultados mostram a capacidade dos estilos de decisão em predizer maior planejamento para a aposentadoria. O terceiro artigo teve como objetivo entender como as três etapas do processo da aposentadoria se relacionam entre si e com variáveis demográficas e de saúde mental. Os resultados apontaram que as variáveis que influenciam o processo pré-transição da aposentadoria são mutáveis dependendo da dimensão de planejamento a ser observada, mas a predição das percepções e dos motivos para aposentar e os estilos de decisão sobre o planejamento foi confirmada em todas as 4 dimensões (saúde, estilo de vida, psicossocial e finanças). De forma geral, o estudo permitiu verificar a existência da tríade pré-transição para a aposentadoria no Brasil, enfocando a influência de fatores demográficos, como a idade, e pessoais, como as percepções, o estilo de decisão e os motivos para aposentar no sequente planejamento para a vida pós trabalho. Destaca-se a necessidade de pesquisas adicionais, a fim de verificar a existência de outras variáveis psicossociais que possam estar relacionadas ao processo da aposentadoria nos trabalhadores brasileiros. Palavras-Chave: Aposentadoria; Planejamento da Aposentadoria; Carreira; Envelhecimento; Escalas Psicométricas

Performance of a small size telescope (SST-1M) camera for gamma-ray astronomy with the Cherenkov Telescope Array

The foreseen implementations of the Small Size Telescopes (SST) in CTA will provide unique insights into the highest energy gamma rays offering fundamental means to discover and under- stand the sources populating the Galaxy and our local neighborhood. Aiming at such a goal, the SST-1M is one of the three different implementations that are being prototyped and tested for CTA. SST-1M is a Davies-Cotton single mirror telescope equipped with a unique camera technology based on SiPMs with demonstrated advantages over classical photomultipliers in terms of duty-cycle. In this contribution, we describe the telescope components, the camera, and the trigger and readout system. The results of the commissioning of the camera using a dedicated test setup are then presented. The performances of the camera first prototype in terms of expected trigger rates and trigger efficiencies for different night-sky background conditions are presented, and the camera response is compared to end-to-end simulations.Comment: All CTA contributions at arXiv:1709.0348

Development of a strategy for calibrating the novel SiPM camera of the SST-1M telescope proposed for the Cherenkov Telescope Array

CTA will comprise a sub-array of up to 70 small size telescopes (SSTs) at the southern array. The SST-1M project, a 4 m-diameter Davies Cotton telescope with 9 degrees FoV and a 1296 pixels SiPM camera, is designed to meet the requirements of the next generation ground based gamma-ray observatory CTA in the energy range above 3 TeV. Silicon photomultipliers (SiPM) cameras of gamma-ray telescopes can achieve good performance even during high night sky background conditions. Defining a fully automated calibration strategy of SiPM cameras is of great importance for large scale production validation and online calibration. The SST-1M sub-consortium developed a software compatible with CTA pipeline software (CTApipe). The calibration of the SST-1M camera is based on the Camera Test Setup (CTS), a set of LED boards mounted in front of the camera. The CTS LEDs are operated in pulsed or continuous mode to emulate signal and night sky background respectively. Continuous and pulsed light data analysis allows us to extract single pixel calibration parameters to be used during CTA operation.Comment: All CTA contributions at arXiv:1709.0348

Control Software for the SST-1M Small-Size Telescope prototype for the Cherenkov Telescope Array

The SST-1M is a 4-m Davies--Cotton atmospheric Cherenkov telescope optimized to provide gamma-ray sensitivity above a few TeV. The SST-1M is proposed as part of the Small-Size Telescope array for the Cherenkov Telescope Array (CTA), the first prototype has already been deployed. The SST-1M control software of all subsystems (active mirror control, drive system, safety system, photo-detection plane, DigiCam, CCD cameras) and the whole telescope itself (master controller) uses the standard software design proposed for all CTA telescopes based on the ALMA Common Software (ACS) developed to control the Atacama Large Millimeter Array (ALMA). Each subsystem is represented by a separate ACS component, which handles the communication to and the operation of the subsystem. Interfacing with the actual hardware is performed via the OPC UA communication protocol, supported either natively by dedicated industrial standard servers (PLCs) or separate service applications developed to wrap lower level protocols (e.g. CAN bus, camera slow control) into OPC UA. Early operations of the telescope without the camera were already carried out. The camera is fully assembled and is capable to perform data acquisition using artificial light source.Comment: In Proceedings of the 35th International Cosmic Ray Conference (ICRC2017), Busan, Korea. All CTA contributions at arXiv:1709.0348

Use or abuse of bioinformatic tools: A response to Samach

In a recent paper, we described for the first time the effects of fruit on the expression of putative homologues of genes involved in flowering pathways. It was our aim to provide insight into the molecular mechanisms underlying alternate bearing in citrus. However, a bioinformatics-based critique of our and other related papers has been given by Samach in the preceding Viewpoint article in this issue of Annals of Botany. The use of certain bioinformatic tools in a context of structural rather than functional genomics can cast doubts about the veracity of a large amount of data published in recent years. In this response, the contentions raised by Samach are analysed, and rebuttals of his criticisms are presented.Muñoz Fambuena, N.; Mesejo Conejos, C.; Gonzalez Más, MC.; Primo-Millo, E.; Agustí Fonfría, M.; Iglesias, DJ. (2013). Use or abuse of bioinformatic tools: A response to Samach. Annals of Botany. 111(3):335-336. doi:10.1093/aob/mct020S3353361113Aleza, P., Juárez, J., Hernández, M., Pina, J. A., Ollitrault, P., & Navarro, L. (2009). Recovery and characterization of a Citrus clementina Hort. ex Tan. «Clemenules» haploid plant selected to establish the reference whole Citrus genome sequence. BMC Plant Biology, 9(1), 110. doi:10.1186/1471-2229-9-110Huang, X. (1999). CAP3: A DNA Sequence Assembly Program. Genome Research, 9(9), 868-877. doi:10.1101/gr.9.9.868Muñoz-Fambuena, N., Mesejo, C., Carmen González-Mas, M., Primo-Millo, E., Agustí, M., & Iglesias, D. J. (2011). Fruit regulates seasonal expression of flowering genes in alternate-bearing ‘Moncada’ mandarin. Annals of Botany, 108(3), 511-519. doi:10.1093/aob/mcr164Rafalski, J. A. (2002). Plant genomics: Present state and a perspective on future developments. Briefings in Functional Genomics and Proteomics, 1(1), 80-94. doi:10.1093/bfgp/1.1.80Rhee, S. Y. (2005). Bioinformatics. Current Limitations and Insights for the Future: Figure 1. Plant Physiology, 138(2), 569-570. doi:10.1104/pp.104.900153Samach, A. (2012). Congratulations, you have been carefully chosen to represent an important developmental regulator! Annals of Botany, 111(3), 329-333. doi:10.1093/aob/mcs161Vassilev, D., Leunissen, J., Atanassov, A., Nenov, A., & Dimov, G. (2005). Application of Bioinformatics in Plant Breeding. Biotechnology & Biotechnological Equipment, 19(sup3), 139-152. doi:10.1080/13102818.2005.1081729

Empirical Comparison of Simple Sequence Repeats and Single Nucleotide Polymorphisms in Assessment of Maize Diversity and Relatedness

While Simple Sequence Repeats (SSRs) are extremely useful genetic markers, recent advances in technology have produced a shift toward use of single nucleotide polymorphisms (SNPs). The different mutational properties of these two classes of markers result in differences in heterozygosities and allele frequencies that may have implications for their use in assessing relatedness and evaluation of genetic diversity. We compared analyses based on 89 SSRs (primarily dinucleotide repeats) to analyses based on 847 SNPs in individuals from the same 259 inbred maize lines, which had been chosen to represent the diversity available among current and historic lines used in breeding. The SSRs performed better at clustering germplasm into populations than did a set of 847 SNPs or 554 SNP haplotypes, and SSRs provided more resolution in measuring genetic distance based on allele-sharing. Except for closely related pairs of individuals, measures of distance based on SSRs were only weakly correlated with measures of distance based on SNPs. Our results suggest that 1) large numbers of SNP loci will be required to replace highly polymorphic SSRs in studies of diversity and relatedness and 2) relatedness among highly-diverged maize lines is difficult to measure accurately regardless of the marker system

A pipeline for high throughput detection and mapping of SNPs from EST databases

Single nucleotide polymorphisms (SNPs) represent the most abundant type of genetic variation that can be used as molecular markers. The SNPs that are hidden in sequence databases can be unlocked using bioinformatic tools. For efficient application of these SNPs, the sequence set should be error-free as much as possible, targeting single loci and suitable for the SNP scoring platform of choice. We have developed a pipeline to effectively mine SNPs from public EST databases with or without quality information using QualitySNP software, select reliable SNP and prepare the loci for analysis on the Illumina GoldenGate genotyping platform. The applicability of the pipeline was demonstrated using publicly available potato EST data, genotyping individuals from two diploid mapping populations and subsequently mapping the SNP markers (putative genes) in both populations. Over 7000 reliable SNPs were identified that met the criteria for genotyping on the GoldenGate platform. Of the 384 SNPs on the SNP array approximately 12% dropped out. For the two potato mapping populations 165 and 185 SNPs segregating SNP loci could be mapped on the respective genetic maps, illustrating the effectiveness of our pipeline for SNP selection and validation

Extent and structure of linkage disequilibrium in canola quality winter rapeseed (Brassica napus L.)

Linkage disequilibrium was investigated in canola quality winter rapeseed to analyze (1) the prospects for whole-genome association analyses and (2) the impact of the recent breeding history of rapeseed on linkage disequilibrium. A total of 845 mapped AFLP markers with allele frequencies ≥0.1 were used for the analysis of linkage disequilibrium in a population of 85 canola quality winter rapeseed genotypes. A low overall level of linkage disequilibrium was found with a mean r2 of only 0.027 over all 356,590 possible marker pairs. At a significance threshold of P = 2.8 × 10−7, which was derived by a Bonferroni correction from a global α-level of 0.1, only 0.78% of the marker pairs were in significant linkage disequilibrium. Among physically linked marker pairs, the level of linkage disequilibrium was about five times higher with more than 10% of marker pairs in significant linkage disequilibrium. Linkage disequilibrium decayed rapidly with distance between linked markers with high levels of linkage disequilibrium extending only for about 2 cM. Owing to the rapid decay of linkage disequilibrium with distance association analyses in canola quality rapeseed will have a significantly higher resolution than QTL analyses in segregating populations by interval mapping, but much larger number of markers will be necessary to cover the whole genome. A major impact of the recent breeding history of rapeseed on linkage disequilibrium could not be observed