Efficacy of biosecurity measures in the control of microorganisms associated to endometritis in sows. Preliminary study

Biosecurity can be defined as all the applied measurements that take as a target to minimize the sanitary risks in a stock farm, and include measurements related to the facilities and the management. The efficacy of these measurements must be reflected in a decrease of the microorganism in different productive phases. A study was carried out to evaluate if the set of applied measurements influences the microbial uterine contamination after farrowing of healthy sows. Two swine farms were been completed about biosecurity measurements was completed and a microbiological study of uterine swabs of sows after the farrowing was carried out. A total of 60 animals were studied, and 27 (45%, 95% CI [33.3%, 56.7%]) resulted positive. Significant differences between production and selection and multiplication farms were detected (OR = 3.44, IC 95%, 1.135-11.047). The colonization frequency was 65% CI [51.3%, 78.6%] and 35% CI [21%, 49%] in production and selection farm, respectively (P = 0.02). A total of 66 isolates were obtained, represented mainly by Staphylococcus spp. (33.33%) and Aerococcus spp. (27.27%), although other species included in the genus Streptococcus (9.09%), Enterococcus (6.06%) and Pseudomonas (4.55%), as well as different fungi species were also isolated. The frequency of isolation of different microorganisms was similar in both farms, with the exception of the genus Enterococcus that was not isolated in the production farm (P = 0.01). The questionnaire showed some differences in biosecurity measures in the selection and multiplication farm when it is compared to the production farm, which together with the increased uterine microbial contamination observed in the latter leads us to propose a preliminary hypothesis about the possible risk factors associated with this process, highlighting the absence of measures to avoid the presence of vectors and the establishment of strict protocols for cleaning and disinfectio

Risk factors associated with the antimicrobial resistance of staphylococci in canine pyoderma

This study reports the susceptibility to antimicrobial agents of staphylococci 4 (n=105) isolated from dogs, and the factors associated with this resistance. The study 5 animals were 23 healthy dogs (group A), 24 with first-time pyoderma (group B), and 27 6 with recurrent pyoderma that had undergone long-term antibiotic treatment (group C). 7 Staphylococci were more commonly isolated from the pyoderma-affected than the 8 healthy dogs (p<0.0001). 9 10 Some 78% of the isolates were resistant to at least one antimicrobial agent. 11 Resistance to amoxicillin-clavulanate,cephalosporins (OR 4.29, 95% CI [1.15, 16.3] 12 respectively), enrofloxacin (OR 9.47, 95% CI [1.53, 58.5]) and ciprofloxacin (OR 79.7 13 95% CI [3.26, 1947.4]) was more common among group C isolates. Some 32% of all 14 the isolates were multiresistant (MR) and 10.4% were methicillin-resistant (MRS). The 15 probability of isolating MRS staphylococci in group C increased by a factor of four 16 (95% CI [1.18, 17.9]) compared to A plus B. Multi-resistant (MR) isolates were 17 obtained more commonly from urban than rural dogs (OR 3.79, 95% CI [1.09, 13.17]). 18 All the MRS staphylococci encountered were obtained from urban dogs and more 19 commonly from male dogs (p= 0.07). 20 21 This study shows that dogs bred in urban habitat, with a history of antibiotic 22 therapy in the past year represents significant risk of being carriers of isolates resistant 23 to methicillin (MRS) and other antimicrobials. These factors should be considered 24 before applying an antimicrobial treatment in veterinary clinics

Cortometrajes para el laboratorio docente LABFIS

Alumnos de la asignatura Física de muchos primeros cursos de los nuevos Grados en que imparte docencia nuestro departamento provienen del Bachillerato de Ciencias de la Salud, con una buena base en Química y Biología, pero no en Física y Matemáticas. Esto es especialmente cierto en Grados como Ciencias Ambientales, Bioquímica, Biología, Química, Ingeniero Químico e Ingeniero de Salud. Para estos alumnos, realizar en el laboratorio experimentos intuitivos que ilustren buena parte del core teórico del curso es una forma efectiva de comprender y asimilar la teoría. Para ello, en nuestras asignaturas de Física (típicamente de 6 créditos), programamos un crédito presencial de laboratorio, de forma que los alumnos visitan el laboratorio en cinco sesiones de dos horas. En esta comunicación se expone el trabajo realizado a través de un proyecto de innovación educativa, mediante el cual se ha desarrollado material multimedia de apoyo al laboratorio docente de Física LABFIS. Este material consiste en vídeos explicativos de corta duración, a través de los cuales el alumno es capaz de desarrollar de manera autosuficiente la práctica correspondiente en el laboratorio.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Productive and sanitary characterization of suckling calves breeding in Córdoba and Santa Fé (Argentina)

A study has been made in 72 dairy farms in the Argentine provinces of Cordoba and Santa Fe between years 2000 and 2002. This article is mainly referring to the calves in suckling stage, before its transfer to the pasture. The objective was to make a valuation of the main charac-teristics of farms and the breeding system of dairy calves in the zone. A questionnaire was designed, structuring the questions in six different sections: questions of general type, workers, feeding, handling, other species and contagious, and biosecurity and health. Among the results, it emphasizes the big size of farms, the great diffusion of open systems and the little pursuit of biosecurity measures.Se ha realizado un estudio, entre los años 2000 y 2002, en 72 explotaciones bovinas de aptitud láctea situadas en las provincias argentinas de Córdoba y Santa Fé. El estudio se centró principalmente en las primeras fases de producción de terneros, previamente a su traslado a los pastos. El objetivo fue realizar una valoración de las principales características de las explotaciones y el sistema de cría de terneros en la zona. Para ello, se diseñó un cuestionario, estructu-rándose las preguntas en seis apartados: cuestiones de tipo general, personal, alimentación, prácticas de manejo, otras especies y contagio y, bioseguridad y sanidad. Entre los resultados, destaca el gran tamaño de las explotaciones, la amplia difusión de sistemas abiertos y el escaso seguimiento de medidas de bioseguridad

Multi-Etiological Nature of Tuberculosis-Like Lesions in Condemned Pigs at the Slaughterhouse.

Tuberculosis-like lesions (TBL) in pigs have been associated with microorganisms other than mycobacteria. In this work a histopathological and microbiological evaluation of TBL in pigs is shown. A total of 352 samples belonging to 171 pigs totally condemned at slaughterhouse due to generalized TBL were sampled and selected for analysis. Pyogranulomatous (56.2%) and granulomatous lesions (20.2%) were observed in all analysed organs. Most of the granulomas observed in both lymph nodes and lungs belonged to more advanced stages of development (stages III and IV) whereas in the liver and the spleen most of lesions belonged to intermediate stages (stages II and III). Different microorganisms were simultaneously detected from TBL in the 42.7% of the animals. Mycobacterium tuberculosis complex (MTC) (38%), coryneform bacteria (40.3%) and streptococci (28.1%) were the main groups of microorganisms detected after bacteriological analysis, with Trueperella pyogenes and Streptococcus suis as the most frequently isolated species. Mycobacteria belonging to MTC were the most frequently detected pathogens in granulomatous and pyogranulomatous lesions in submandibular lymph nodes (32.7%) and coryneform bacteria were the microorganisms more frequently isolated from lungs (25.9%) and spleen samples (37.2%). These results may provide new insights into the pathogenesis and diagnosis of this pathology. The importance of coryneform bacteria and streptococci in such processes must be evaluated in future studies