Evaluation of Immobilized Boronates for Studies of Adenine Nucleotide Metabolism

Immobilized boronates were evaluated for studies of adenine nucleotide metabolism. These studies were performed using Affi-gel 601, a commercial boronate gel, and dihydroxyboryl Sepharose and dihydroxyboryl-Bio Rex which were synthesized in the laboratory. The studies performed included the determination of the relative binding affinity of a variety of adenine containing compounds for the three immobilized boronates under differing chromatographic conditions

Complex Polymers of ADP-Ribose Occur in Vitro and in Vivo

The work presented here included the development of a highly sensitive method to estimate the size and complexity of poly(ADP-ribose). This involved radiolabeling of the precursor pools, purification of polymers using a boronate resin, polymer fractionation according to size by molecular sieve chromatography and analysis of polymer complexity by enzymatic digestion to nucleotides which were quantified by strong anion exchange chromatography

Silica final lenses under HiPER laser fusion reactor operation conditions

We have studied the thermo-mechanical response and atomistic degradation of final lenses in HiPER project. Final silica lenses are squares of 75 × 75 cm2 with a thickness of 5 cm. There are two scenarios where lenses are located at 8 m from the centre: •HiPER 4a, bunches of 100 shots (maximum 5 DT shots <48 MJ at ≈0.1 Hz). No blanket in chamber geometry. •HiPER 4b, continuous mode with shots ≈50 MJ at 10 Hz to generate 0.5 GW. Liquid metal blanket in chamber design

Stabilization of phenolic compounds from opuntia oligacantha först by microencapsulation with agave sap (aguamiel)

The aim of this research was to determine the stability of phenolic compounds from Opuntia oligacantha Forst (xoconostle) ¨ by microencapsulation with a blend of biopolymers (maltodextrin and gum arabic) and agave sap (aguamiel) as a thermoprotector. The particle size distribution, morphology, stability during storage at different temperatures and water activity of the microcapsules were determined. The results showed significant differences (P<0.05) among the microcapsules. Higher protection was found in the microcapsules containing aguamiel. The microcapsules had a spherical shape with an average diameter of 7.72 µm. It was observed that the microcapsules containing aguamiel had a minor change in colour independent of the drying temperature and preserved the phenolic compounds for more than 1467 days at a storage temperature of 25 °C. These results suggest the application of microencapsulation with phenolic compounds from xoconostle for food products. Keywords: biopolymers, xoconostle, stability, spray drying

Lifetime of silica final lenses subject to HiPER irradiation conditions

The goal of the European laser fusion project, is to build an engineering facility for repetitive laser operation (HiPER 4a) and later a fusion reactor (HiPER 4b). A key aspect for laser fusion energy is the final optics. At the moment, it is based on silica transmission lenses located 8 m away from the chamber center. Lens lifetime depends on the irradiation conditions. We have used a 48 MJ shock ignition target for calculations. We have studied the thermo-mechanical effects of ions and X-rays on the lenses. Ions lead to lens melting and must therefore be mitigated. On the other hand, X-rays (~1% of the energy) does not produce either a significant temperature rise or detrimental stresses. Finally, we calculated the neutron flux and gamma dose rate on the lenses. Next, based on a simple model we studied the formation of color centers in the sample, which lead to optical absorption. Calculations show that simultaneous neutron and gamma irradiation does not significantly increase the optical absorption during the expected lifetime of the HiPER 4a facility. Under severe conditions (HiPER 4b), operation above 800 K or lens refreshing by thermal annealing treatments seem to assure adequate behavior

A hybrid Crank-Nicolson FDTD subgridding boundary condition for lossy thin-layer modelling

The inclusion of thin lossy, material layers, such as carbon based composites, is essential for many practical applications modeling the propagation of electromagnetic energy through composite structures such as those found in vehicles and electronic equipment enclosures. Many existing schemes suffer problems of late time instability, inaccuracy at low frequency, and/or large computational costs. This work presents a novel technique for the modeling of thin-layer lossy materials in FDTD schemes which overcomes the instability problem at low computational cost. For this, a 1D-subgrid is used for the spatial discretization of the thin layer material. To overcome the additional time-step constraint posed by the reduction in the spatial cell size, a Crank-Nicolson time-integration scheme is used locally in the subgridded zone, and hybridized with the usual 3D Yee-FDTD method, which is used for the rest of the computational domain. Several numerical experiments demonstrating the accuracy of this approach are shown and discussed. Results comparing the proposed technique with classical alternatives based on impedance boundary condition approaches are also presented. The new technique is shown to have better accuracy at low frequencies, and late time stability than existing techniques with low computational cost

DGTD for a Class of Low-Observable Targets: A Comparison With MoM and (2, 2) FDTD

The simulation of low-observable targets requires high accuracy, both in the geometrical discretization as well as in the numerical solution of the electromagnetic problem. In this letter, we employ the well-known NASA almond, to illustrate the accuracy of the Leap-Frog Discontinuous Galerkin method, combined with a local time stepping algorithm, comparing it with the MoM and the (2,2) FDTD methods.The work described in this paper and the research leading to these results has received funding from the European Community’s Seventh Framework Programme FP7/2007-2013, under grant agreement no 205294 (HIRF SE project), and from the Spanish National Projects TEC2010-20841-C04-04, CSD2008-00068, and the Junta de Andalucia Project P09-TIC-

Computer-assisted dental implant placement following free flap reconstruction: virtual planning, CAD/CAM templates, dynamic navigation and augmented reality

Image-guided surgery, prosthetic-based virtual planning, 3D printing, and CAD/CAM technology are changing head and neck ablative and reconstructive surgical oncology. Due to quality-of-life improvement, dental implant rehabilitation could be considered in every patient treated with curative intent. Accurate implant placement is mandatory for prosthesis long-term stability and success in oncologic patients. We present a prospective study, with a novel workflow, comprising 11 patients reconstructed with free flaps and 56 osseointegrated implants placed in bone flaps or remnant jaws (iliac crest, fibula, radial forearm, anterolateral thigh). Starting from CT data and jaw plaster model scanning, virtual dental prosthesis was designed. Then prosthetically driven dental implacement was also virtually planned and transferred to the patient by means of intraoperative infrared optical navigation (first four patients), and a combination of conventional static teeth supported 3D-printed acrylic guide stent, intraoperative dynamic navigation, and augmented reality for final intraoperative verification (last 7 patients). Coronal, apical, and angular deviation between virtual surgical planning and final guided intraoperative position was measured on each implant. There is a clear learning curve for surgeons when applying guided methods. Initial only-navigated cases achieved low accuracy but were comparable to non-guided freehand positioning due to jig registration instability. Subsequent dynamic navigation cases combining highly stable acrylic static guides as reference and registration markers result in the highest accuracy with a 1-1.5-mm deviation at the insertion point. Smartphone-based augmented reality visualization is a valuable tool for intraoperative visualization and final verification, although it is still a difficult technique for guiding surgery. A fixed screw-retained ideal dental prosthesis was achieved in every case as virtually planned. Implant placement, the final step in free flap oncological reconstruction, could be accurately planned and placed with image-guided surgery, 3D printing, and CAD/CAM technology. The learning curve could be overcome with preclinical laboratory training, but virtually designed and 3D-printed tracer registration stability is crucial for accurate and predictable results. Applying these concepts to our difficult oncologic patient subgroup with deep anatomic alterations ended in comparable results as those reported in non-oncologic patients.This work was supported by grant PI18/01625 (Ministerio de Ciencia e Innovación-Instituto de Salud Carlos III and European Regional Development Fund "Una manera de hacer Europa"). This study was also supported by Ticare® implants (Mozo-Grau, Valladolid, Spain). The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication