Beam shaping to enhance zero group velocity Lamb mode generation in a composite plate and nondestructive testing application

International audienceZero group velocity (ZGV) Lamb modes have already shown their potential in nondestructive testing applications as they are sensitive to the sample structural characteristics. In this paper, we first consider an aluminum sample to validate a method based on the beam shaping of the generation laser. This method is proven to enhance ZGV Lamb modes in aluminum, and then advantageously applied to a composite material plate. Finally, based on the proposed method, scanning the sample over healthy and flawed zones demonstrates the ability to detect subsurface flaws

High-Frequency Elastic Coupling at the Interface of van der Waals Nanolayers Imaged by Picosecond Ultrasonics

Although the topography of van de Waals (vdW) layers and heterostructures can be imaged by scanning probe microscopy, high-frequency interface elastic properties are more difficult to assess. These can influence the stability, reliability and performance of electronic devices that require uniform layers and interfaces. Here, we use picosecond ultrasonics to image these properties in vdW layers and heterostructures based on well-known exfoliable materials, i.e. InSe, hBN and graphene. We reveal a strong, uniform elastic coupling between vdW layers over a wide range of frequencies of up to tens of gigahertz (GHz) and in-plane areas of 100 _m2. In contrast, the vdW layers can be weakly coupled to their supporting substrate, behaving effectively as free standing membranes. Our data and analysis demonstrate that picosecond ultrasonics offers opportunities can probe the high-frequency elastic coupling of vdW nanolayers and image both perfect and broken interfaces between different materials over a wide frequency range, as required for future scientific and technological developments

O-Antigen Delays Lipopolysaccharide Recognition and Impairs Antibacterial Host Defense in Murine Intestinal Epithelial Cells

Although Toll-like receptor (TLR) 4 signals from the cell surface of myeloid cells, it is restricted to an intracellular compartment and requires ligand internalization in intestinal epithelial cells (IECs). Yet, the functional consequence of cell-type specific receptor localization and uptake-dependent lipopolysaccharide (LPS) recognition is unknown. Here, we demonstrate a strikingly delayed activation of IECs but not macrophages by wildtype Salmonella enterica subsp. enterica sv. (S.) Typhimurium as compared to isogenic O-antigen deficient mutants. Delayed epithelial activation is associated with impaired LPS internalization and retarded TLR4-mediated immune recognition. The O-antigen-mediated evasion from early epithelial innate immune activation significantly enhances intraepithelial bacterial survival in vitro and in vivo following oral challenge. These data identify O-antigen expression as an innate immune evasion mechanism during apical intestinal epithelial invasion and illustrate the importance of early innate immune recognition for efficient host defense against invading Salmonella

Relationship between Antibody Susceptibility and Lipopolysaccharide O-Antigen Characteristics of Invasive and Gastrointestinal Nontyphoidal Salmonellae Isolates from Kenya

Background: Nontyphoidal Salmonellae (NTS) cause a large burden of invasive and gastrointestinal disease among young children in sub-Saharan Africa. No vaccine is currently available. Previous reports indicate the importance of the O-antigen of Salmonella lipopolysaccharide for virulence and resistance to antibody-mediated killing. We hypothesised that isolates with more O-antigen have increased resistance to antibody-mediated killing and are more likely to be invasive than gastrointestinal. Methodology/Principal findings: We studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011. Isolates were tested for susceptibility to antibody-mediated killing, using whole adult serum. O-antigen structural characteristics, including O-acetylation and glucosylation, were investigated. Overall, isolates were susceptible to antibody-mediated killing, but S. Enteritidis were less susceptible and expressed more O-antigen than Typhimurium (p\u3c0.0001 for both comparisons). For S. Typhimurium, but not Enteritidis, O-antigen expression correlated with reduced sensitivity to killing (r = 0.29, 95% CI = 0.10-0.45, p = 0.002). Both serovars expressed O-antigen populations ranging 21-33 kDa average molecular weight. O-antigen from most Typhimurium were O-acetylated on rhamnose and abequose residues, while Enteritidis O-antigen had low or no O-acetylation. Both Typhimurium and Enteritidis O-antigen were approximately 20%-50% glucosylated. Amount of S. Typhimurium O-antigen and O-antigen glucosylation level were inversely related. There was no clear association between clinical presentation and antibody susceptibility, O-antigen level or other O-antigen features. Conclusion/Significance: Kenyan S. Typhimurium and Enteritidis clinical isolates are susceptible to antibody-mediated killing, with degree of susceptibility varying with level of O-antigen for S. Typhimurium. This supports the development of an antibody-inducing vaccine against NTS for Africa. No clear differences were found in the phenotype of isolates from blood and stool, suggesting that the same isolates can cause invasive disease and gastroenteritis. Genome studies are required to understand whether invasive and gastrointestinal isolates differ at the genotypic level

Development of numerical methods for the imaging of optoacoustic sources in solid media

L’objectif de ces travaux est le développement d’une méthode d’imagerie de sources optoacoustiques en milieu solide. Afin d’analyser l’influence de la géométrie de la source sur les ondes acoustiques qu’elle génère et ainsi prévoir qualitativement si l’image de la source peut être obtenue avec une précision suffisante, la directivité d’une source acoustique est d’abord considérée. Pour quantifier plus précisément cette influence, la résolution complète de la propagation des ondes générées par cette source est ensuite menée. L’analyse de l’influence de l’incidence oblique d’un faisceau laser lors de la génération photoacoustique est proposée en illustration. La seconde partie de ce manuscrit est consacrée à la résolution du problème inverse qui permet d’obtenir l’image de la source initiale. Un algorithme de rétropropagation est alors mis en place. Il est basé sur les principes du retournement temporel et de Huygens, et simule la propagation, en milieu solide, des ondes mesurées vers la position initiale de la source acoustique.The purpose of this work is to develop a method to image optoacoustic sources in solid media. To analyze the influence of the source geometry on laser-generated acoustic waves and thus qualitatively predict if the image of the source can be obtained with sufficient accuracy, the directivity of an acoustic source is considered first. In order to quantify more precisely this influence, the complete resolution of the propagation of acoustic waves generated by this source is then achieved. The analysis of the effect of oblique incidence of a laser beam in the photoacoustic generation process is proposed as an illustration. The second part of this thesis is devoted to the inverse problem resolution, which provides the image of the initial acoustic source. A backpropagation algorithm is then implemented. It is based on the time-reversal principle and the Huygens’ principle, and simulates, in a solid medium, the propagation of the measured acoustic waves back to the initial position of the acoustic source

Développement de méthodes numériques pour l'imagerie de sources otpoacoustiques en milieu solide

The purpose of this work is to develop a method to image optoacoustic sources in solid media. To analyze the influence of the source geometry on laser-generated acoustic waves and thus qualitatively predict if the image of the source can be obtained with sufficient accuracy, the directivity of an acoustic source is considered first. In order to quantify more precisely this influence, the complete resolution of the propagation of acoustic waves generated by this source is then achieved. The analysis of the effect of oblique incidence of a laser beam in the photoacoustic generation process is proposed as an illustration. The second part of this thesis is devoted to the inverse problem resolution, which provides the image of the initial acoustic source. A backpropagation algorithm is then implemented. It is based on the time-reversal principle and the Huygens' principle, and simulates, in a solid medium, the propagation of the measured acoustic waves back to the initial position of the acoustic source.L'objectif de ces travaux est le développement d'une méthode d'imagerie de sources optoacoustiques en milieu solide. Afin d'analyser l'influence de la géométrie de la source sur les ondes acoustiques qu'elle génère et ainsi prévoir qualitativement si l'image de la source peut être obtenue avec une précision suffisante, la directivité d'une source acoustique est d'abord considérée. Pour quantifier plus précisément cette influence, la résolution complète de la propagation des ondes générées par cette source est ensuite menée. L'analyse de l'influence de l'incidence oblique d'un faisceau laser lors de la génération photoacoustique est proposée en illustration. La seconde partie de ce manuscrit est consacrée à la résolution du problème inverse qui permet d'obtenir l'image de la source initiale. Un algorithme de rétropropagation est alors mis en place. Il est basé sur les principes du retournement temporel et de Huygens, et simule la propagation, en milieu solide, des ondes mesurées vers la position initiale de la source acoustique

Table ronde

Temps de discussion à l'issue de la session <i>Ultrasons laser, interactions son-lumière</i>

Développement de méthodes numériques pour l'imagerie de sources optoacoustiques en milieu solide

L objectif de ces travaux est le développement d une méthode d imagerie de sources optoacoustiques en milieu solide. Afin d analyser l influence de la géométrie de la source sur les ondes acoustiques qu elle génère et ainsi prévoir qualitativement si l image de la source peut être obtenue avec une précision suffisante, la directivité d une source acoustique est d abord considérée. Pour quantifier plus précisément cette influence, la résolution complète de la propagation des ondes générées par cette source est ensuite menée. L analyse de l influence de l incidence oblique d un faisceau laser lors de la génération photoacoustique est proposée en illustration. La seconde partie de ce manuscrit est consacrée à la résolution du problème inverse qui permet d obtenir l image de la source initiale. Un algorithme de rétropropagation est alors mis en place. Il est basé sur les principes du retournement temporel et de Huygens, et simule la propagation, en milieu solide, des ondes mesurées vers la position initiale de la source acoustique.The purpose of this work is to develop a method to image optoacoustic sources in solid media. To analyze the influence of the source geometry on laser-generated acoustic waves and thus qualitatively predict if the image of the source can be obtained with sufficient accuracy, the directivity of an acoustic source is considered first. In order to quantify more precisely this influence, the complete resolution of the propagation of acoustic waves generated by this source is then achieved. The analysis of the effect of oblique incidence of a laser beam in the photoacoustic generation process is proposed as an illustration. The second part of this thesis is devoted to the inverse problem resolution, which provides the image of the initial acoustic source. A backpropagation algorithm is then implemented. It is based on the time-reversal principle and the Huygens principle, and simulates, in a solid medium, the propagation of the measured acoustic waves back to the initial position of the acoustic source.BORDEAUX1-Bib.electronique (335229901) / SudocSudocFranceF

Oblique laser incidence to select laser-generated acoustic modes

The purpose of this paper is to study the effect of a non-normal optical penetration due to an obliquely incident laser source. It is shown that the loss of symmetry due to such a penetration influences specific bulk acoustic modes. For a given detection position, increase in shear wave amplitude is obtained by orienting the incident laser source. 1. Introduction Since the first quantitative approaches in the 60's [1], a large number of studies has been conducted to better understand the generation of laser-generated acoustic waves. Models for the acoustic generation in the thermoelastic regime, taking into account the optical penetration of the source [2] or the effect of source width [3], were developed. A more general approach based on Green's function formalism also included thermal diffusion in the medium [4]. These works have dealt with the modeling of a circular spot of laser illumination. However, modeling of the acoustic field generated by a line source is of interest, since the signal-to-noise ratio may be increased in this experimental situation [5]. All the works cited above assume a normal incidence of the laser beam with respect to the illuminated surface of the sample. The purpose of this paper is to analyse the effects of oblique incidence on acoustic waves generated by a line-focused laser source. We present a theoretical model accounting for the effects of optical penetration, finite width of the beam and pulse duration of the laser source. The absorbed energy density is first calculated by solving Maxwell's equations. Then the thermoelastic equations are solved to obtain the displacement field through a semi-analytical calculation based on a double Fourier transform in space and time. It is shown that oblique incidence increases shear wave amplitude. Changes of the longitudinal waveforms are also discussed