Síndrome hepatocutáneo : revisión bibliográfica y estudio retrospectivo de tres casos clínicos

El síndrome hepatocutáneo o dermatitis superficial necrolítica es una afección del perro que se manifiesta con lesiones costrosas y ulcerativas en la zona distal de las extremidades, en la cara y en los genitales externos. Estas lesiones cutáneas coinciden con lesiones hepáticas y con una disminución de los niveles de aminoácidos en sangre. En este trabajo se describen tres casos de perros afectados con este síndrome y se realiza un paralelismo con el eritema necrolítico migratorio descrito en medicina humana.The hepatocutaneous syndrome or superficial necrolytic dermatitis, is a dogs affection which is manifested with crusting and ulceration lesions in distal extremities on the face and the external genitals. These cutaneous lesions coincide together with hepatic lesions and decreased blood amino acid levels. In this article three cases of dogs with this syndrome are described. One comparison can be made with necrolytic migratory erythema described in human medecine

Laringitis granulomatosa en un perro

Perra mestiza de 13 años de edad con un historial de disnea aguda, debida a una obstrucción laríngea causada por una laringitis granulomatosa. Enfermedad de causa desconocida, posiblemente similar a la sarcoidosis humana

Hemangiosarcoma pulmonar primario en un Pastor Alemán con neumotórax espontáneo

Se describe un caso clínico de un hemangiosarcoma (HSA) primario pulmonar, en un pastor alemán, evaluado por un cuadro agudo de disnea asociado a un neumotórax espontáneo.

Mycobacteria emulsified in olive oil-in-water trigger a robust immune response in bladder cancer treatment

The hydrophobic composition of mycobacterial cell walls leads to the formation of clumps when attempting to resuspend mycobacteria in aqueous solutions. Such aggregation may interfere in the mycobacteria-host cells interaction and, consequently, influence their antitumor effect. To improve the immunotherapeutic activity of Mycobacterium brumae, we designed different emulsions and demonstrated their efficacy. The best formulation was initially selected based on homogeneity and stability. Both olive oil (OO)- and mineral oil-in-water emulsions better preserved the mycobacteria viability and provided higher disaggregation rates compared to the others. But, among both emulsions, the OO emulsion increased the mycobacteria capacity to induce cytokines' production in bladder tumor cell cultures. The OO-mycobacteria emulsion properties: less hydrophobic, lower pH, more neutralized zeta potential, and increased affinity to fibronectin than non-emulsified mycobacteria, indicated favorable conditions for reaching the bladder epithelium in vivo. Finally, intravesical OO-M. brumae-treated mice showed a significantly higher systemic immune response, together with a trend toward increased tumor-bearing mouse survival rates compared to the rest of the treated mice. The physicochemical characteristics and the induction of a robust immune response in vitro and in vivo highlight the potential of the OO emulsion as a good delivery vehicle for the mycobacterial treatment of bladder cancer

The tumor suppressor SirT2 regulates cell cycle progression and genome stability by modulating the mitotic deposition of H4K20 methylation

The establishment of the epigenetic mark H4K20me1 (monomethylation of H4K20) by PR-Set7 during G2/M directly impacts S-phase progression and genome stability. However, the mechanisms involved in the regulation of this event are not well understood. Here we show that SirT2 regulates H4K20me1 deposition through the deacetylation of H4K16Ac (acetylation of H4K16) and determines the levels of H4K20me2/3 throughout the cell cycle. SirT2 binds and deacetylates PR-Set7 at K90, modulating its chromatin localization. Consistently, SirT2 depletion significantly reduces PR-Set7 chromatin levels, alters the size and number of PR-Set7 foci, and decreases the overall mitotic deposition of H4K20me1. Upon stress, the interaction between SirT2 and PR-Set7 increases along with the H4K20me1 levels, suggesting a novel mitotic checkpoint mechanism. SirT2 loss in mice induces significant defects associated with defective H4K20me1-3 levels. Accordingly, SirT2-deficient animals exhibit genomic instability and chromosomal aberrations and are prone to tumorigenesis. Our studies suggest that the dynamic cross-talk between the environment and the genome during mitosis determines the fate of the subsequent cell cycle

Citología del sistema linfático aplicada a la inspección sanitaria de carnes en mataderos

El estudio citológico de los ganglios linfáticos es una herramienta de gran valor diagnóstico en la inspección sanitaria de carnes en mataderos por los veterinarios oficiales. No sólo permite descubrir procesos patológicos que sufre el animal, sino que tiene también valor en la investigación de determinados residuso en la carne

Citología del sistema linfático aplicada a la inspección sanitaria de carnes en mataderos

El estudio citológico de los ganglios linfáticos es una herramienta de gran valor diagnóstico en la inspección sanitaria de carnes en mataderos por los veterinarios oficiales. No sólo permite descubrir procesos patológicos que sufre el animal, sino que tiene también valor en la investigación de determinados residuso en la carne