The Effect of Epstein-Barr Virus Latent Membrane Protein 2 Expression on the Kinetics of Early B Cell Infection

Infection of human B cells with wild-type Epstein-Barr virus (EBV) in vitro leads to activation and proliferation that result in efficient production of lymphoblastoid cell lines (LCLs). Latent Membrane Protein 2 (LMP2) is expressed early after infection and previous research has suggested a possible role in this process. Therefore, we generated recombinant EBV with knockouts of either or both protein isoforms, LMP2A and LMP2B (Δ2A, Δ2B, Δ2A/Δ2B) to study the effect of LMP2 in early B cell infection. Infection of B cells with Δ2A and Δ2A/Δ2B viruses led to a marked decrease in activation and proliferation relative to wild-type (wt) viruses, and resulted in higher percentages of apoptotic B cells. Δ2B virus infection showed activation levels comparable to wt, but fewer numbers of proliferating B cells. Early B cell infection with wt, Δ2A and Δ2B viruses did not result in changes in latent gene expression, with the exception of elevated LMP2B transcript in Δ2A virus infection. Infection with Δ2A and Δ2B viruses did not affect viral latency, determined by changes in LMP1/Zebra expression following BCR stimulation. However, BCR stimulation of Δ2A/Δ2B cells resulted in decreased LMP1 expression, which suggests loss of stability in viral latency. Long-term outgrowth assays revealed that LMP2A, but not LMP2B, is critical for efficient long-term growth of B cells in vitro. The lowest levels of activation, proliferation, and LCL formation were observed when both isoforms were deleted. These results suggest that LMP2A appears to be critical for efficient activation, proliferation and survival of EBV-infected B cells at early times after infection, which impacts the efficient long-term growth of B cells in culture. In contrast, LMP2B did not appear to play a significant role in these processes, and long-term growth of infected B cells was not affected by the absence of this protein. © 2013 Wasil et al

Gamma-induced background in the KATRIN main spectrometer

International audienceThe KArlsruhe TRItium Neutrino (KATRIN) experiment aims to make a model-independent determination of the effective electron antineutrino mass with a sensitivity of 0.2 eV/c 2 . It investigates the kinematics of β -particles from tritium β -decay close to the endpoint of the energy spectrum. Because the KATRIN main spectrometer (MS) is located above ground, muon-induced backgrounds are of particular concern. Coincidence measurements with the MS and a scintillator-based muon detector system confirmed the model of secondary electron production by cosmic-ray muons inside the MS. Correlation measurements with the same setup showed that about 12% of secondary electrons emitted from the inner surface are induced by cosmic-ray muons, with approximately one secondary electron produced for every 17 muon crossings. However, the magnetic and electrostatic shielding of the MS is able to efficiently suppress these electrons, and we find that muons are responsible for less than 17% (90% confidence level) of the overall MS background

Muon-induced background in the KATRIN main spectrometer

The KArlsruhe TRItium Neutrino (KATRIN) experiment aims to make a model-independent determination of the effective electron antineutrino mass with a sensitivity of 0.2 eV/c 2 . It investigates the kinematics of β-particles from tritium β-decay close to the endpoint of the energy spectrum. Because the KATRIN main spectrometer (MS) is located above ground, muon-induced backgrounds are of particular concern. Coincidence measurements with the MS and a scintillator-based muon detector system confirmed the model of secondary electron production by cosmic-ray muons inside the MS. Correlation measurements with the same setup showed that about 12% of secondary electrons emitted from the inner surface are induced by cosmic-ray muons, with approximately one secondary electron produced for every 17 muon crossings. However, the magnetic and electrostatic shielding of the MS is able to efficiently suppress these electrons, and we find that muons are responsible for less than 17% (90% confidence level) of the overall MS background