Soils of Tropical Dry Forest and with Different Crops Presenting Ascospores of Monosporascus cannonballus

The vine decline caused by Monosporascus cannonballus is a limiting factor in different crops in several countries. The objective of this study was to quantify the M. cannonballus ascospores in soils covered with tropical dry forest and areas cultivated with pineapple, cotton, coconut, corn, mango, melon, papaya, sorghum and watermelon. Five areas were sampled in tropical dry forest and every crop. The M. cannonballus ascospores were extracted using the flotation method of sucrose. Ascospores of M. cannonballus were detected in all soil samples from Rio Grande do Norte and Ceará states, including tropical dry forest. There were significant differences among the ascospores densities of M. cannonballus, which varied from 0.55 to 2.21 ascospores g-1 soil. The lower densities were found in areas with cotton, coconut, mango, pineapple, and melon within the first and fifth years of cultivation, in addition to uncultivated areas of tropical dry forest. The highest ascospores density was found in papaya areas. Up to date, there is no study to prove that this crop is considered host of this phytopathogen. Cultivated areas with cucurbitaceous with more years of cultivation presented higher densities of M. cannonballus ascospores in soils from Brazilian semiarid. However, there is no direct relationship between M. cannonballus population density in the soil and the susceptibility of the host being cultivated in the soil at the time of sampling

Thrombosed great saphenous vein aneurysm accompanied by venous thrombosis

Superficial venous aneurysms of the lower extremities are considered rare and their clinical significance is poorly defined. The purpose of this article is to report a case of a 72- year-old woman with a thrombosed great saphenous vein aneurysm along with deep venous thrombosis and review its clinical presentation, diagnosis and treatment

Naloxone Prolongs Abdominal Constriction Writhing-Like Behavior in a Zebrafish-Based Pain Model

The ability to detect noxious stimuli is essential to survival. However, pathological pain is maladaptive and severely debilitating. Endogenous and exogenous opioids modulate pain responses via opioid receptors, reducing pain sensibility. Due to the high genetic and physiological similarities to rodents and humans, the zebrafish is a valuable tool to assess pain responses and the underlying mechanisms involved in nociception. Although morphine attenuates pain-like responses of zebrafish, there are no data showing if the antagonism of opioid receptors prolongs pain duration in the absence of an exogenous opioid. Here, we investigated whether a common opioid antagonist naloxone affects the abdominal constriction writhing-like response, recently characterized as a zebrafish-based pain behavior. Animals were injected intraperitoneally with acetic acid (5.0%), naloxone (1.25 mg/kg; 2.5 mg/kg; 5.0 mg/kg) or acetic acid with naloxone to investigate the changes in their body curvature for 1 h. Acetic acid elicited a robust pain-like response in zebrafish, as assessed by aberrant abdominal body curvature, while no effects were observed following PBS injection. Although naloxone alone did not alter the frequency and duration of this behavior, it dose-dependently prolonged acetic acid-induced abdominal curvature response. Besides reinforcing the use of the abdominal writhing-like phenotype as a behavioral endpoint to measure acute pain responses in zebrafish models, our novel data suggest a putative role of endogenous opioids in modulating the recovery from pain stimulation in zebrafish. © 2019 Elsevier B.V.We recognize the financial support and fellowships from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) . F.V.C. was recipient of CAPES fellowship. J.C. and F.V.S. receive the CNPq fellowship. D.B.R. is a recipient of CNPq research productivity grant ( 305051/2018-0 ) and his work is also supported by the PROEX/CAPES (process number 23038.005848/2018-31) and PRONEM/FAPERGS (process number 16/2551-0000248-7) fellowship grants. A.V.K. is the Chair of the International Zebrafish Neuroscience Research Consortium (ZNRC). His research is supported by the Russian Science Foundation (RSF) grant 19-15-00053. All authors contributed to the preparation of the manuscript and approved its final version. The funders had no influence on the study design, collection, analysis, and interpretation of data, as well as on writing and submission of this manuscript

IDH1 mutations in a Brazilian series of Glioblastoma

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Albert Einstein Jewish HospitalUniversidade de São Paulo Faculdade de Medicina Department of NeurologyUniversidade de São Paulo Faculdade de Medicina Department of PathologyCancer Institute of São PauloFundação Pio XII Barretos Cancer HospitalFederal University of São Paulo School of Medicine Department of NeurologyFederal University of São Paulo School of Medicine Department of PathologyNove de Julho HospitalAlbert Einstein Jewish HospitalUNIFESP, EPM, Department of NeurologyUNIFESP, EPM, Department of PathologyFAPESP: 04/12133-6SciEL