Laboratory evaluation of stable isotope labeling of Culicoides (Diptera: Ceratopogonidae) for adult dispersal studies.

BackgroundStable isotope labeling is a promising method for use in insect mark-capture and dispersal studies. Culicoides biting midges, which transmit several important animal pathogens, including bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV), are small flies that develop in various semi-aquatic habitats. Previous Culicoides dispersal studies have suffered from the limitations of other labeling techniques, and an inability to definitively connect collected adult midges to specific immature development sites.ResultsAdult C. sonorensis were successfully labeled with 13C and 15N stable isotopes as larvae developing in a semi-aquatic mud substrate in the laboratory. High and low-dose isotope treatments for both elements significantly enriched midges above the background isotope levels of unenriched controls. Enrichment had no effect on C. sonorensis survival, though a slight (~ 5 day) delay in emergence was observed, and there was no significant effect of pool size on 13C or 15N enrichment levels.ConclusionsStable isotope labeling is life-long, and does not interfere with natural insect behaviors. Stable isotope enrichment using 13C or 15N shows promise for Culicoides dispersal studies in the field. This method can be used to identify adult dispersal from larval source habitat where a midge developed. It may be possible to detect a single enriched midge in a pool of unenriched individuals, though further testing is needed to confirm the sensitivity of this method

Roughness of Crack Interfaces in Two-Dimensional Beam Lattices

The roughness of crack interfaces is reported in quasistatic fracture, using an elastic network of beams with random breaking thresholds. For strong disorders we obtain 0.86(3) for the roughness exponent, a result which is very different from the minimum energy surface exponent, i.e., the value 2/3. A cross-over to lower values is observed as the disorder is reduced, the exponent in these cases being strongly dependent on the disorder.Comment: 9 pages, RevTeX, 3 figure

Sea Water Radiocarbon Evolution in the Gulf of Alaska: 2002 Observations

Oceanic uptake and transport of bomb radiocarbon as {sup 14}CO{sub 2} created by atmospheric nuclear weapons testing in the 1950s and 1960s has been a useful diagnostic to determine the carbon transfer between the ocean and atmosphere. In addition, the distribution of radiocarbon in the ocean can be used as a tracer of oceanic circulation. Results obtained from samples collected in the Gulf of Alaska in the summer of 2002 provide a direct comparison with results in the 1970s during GEOSECS and in the early 1990s during WOCE. The open gyre values are 20-40{per_thousand} more negative than those documented in 1991 and 1993 (WOCE) although the general trends as a function of latitude are reproduced. Surface values are still significantly higher than pre-bomb levels ({approx}-105{per_thousand} or lower). In the central gyre, we observe {Delta}{sup 14}C-values that are lower in comparison to GEOSECS (stn 218) and WOCE P16/P17 to a density of {approx}26.8{sigma}t. This observation is consistent with the overall decrease in surface {Delta}{sup 14}C values, and reflects the erosion of the bomb-{sup 14}C transient. We propose that erosion of the bomb-{sup 14}C transient is accomplished by entrainment of low {sup 14}C water via vertical exchange within the Gulf of Alaska and replenishment of surface and sub-thermocline waters with waters derived from the far northwest Pacific

DART-RAY: a 3D ray-tracing radiative transfer code for calculating the propagation of light in dusty galaxies

We present DART-Ray, a new ray-tracing 3D dust radiative transfer (RT) code designed specifically to calculate radiation field energy density (RFED) distributions within dusty galaxy models with arbitrary geometries. In this paper, we introduce the basic algorithm implemented in . DART-Ray which is based on a pre-calculation of a lower limit for the RFED distribution. This pre-calculation allows us to estimate the extent of regions around the radiation sources within which these sources contribute significantly to the RFED. In this way, ray-tracing calculations can be restricted to take place only within these regions, thus substantially reducing the computational time compared to a complete ray-tracing RT calculation. Anisotropic scattering is included in the code and handled in a similar fashion. Furthermore, the code utilizes a Cartesian adaptive spatial grid and an iterative method has been implemented to optimize the angular densities of the rays originated from each emitting cell. In order to verify the accuracy of the RT calculations performed by DART-Ray, we present results of comparisons with solutions obtained using the dusty 1D RT code for a dust shell illuminated by a central point source and existing 2D RT calculations of disc galaxies with diffusely distributed stellar emission and dust opacity. Finally, we show the application of the code on a spiral galaxy model with logarithmic spiral arms in order to measure the effect of the spiral pattern on the attenuation and RFED. © 2014 The Authors Published by Oxford University Press on behalf of the Royal Astronomical Society

Efficient transplacental IgG transfer in women infected with Zika virus during pregnancy

Zika virus (ZIKV) is a newly-identified infectious cause of congenital disease. Transplacental transfer of maternal IgG to the fetus plays an important role in preventing many neonatal infections. However, antibody transfer may also have negative consequences, such as mediating enhancement of flavivirus infections in early life, or trafficking of virus immune complexes to the fetal compartment. ZIKV infection produces placental pathology which could lead to impaired IgG transfer efficiency as occurs in other maternal infections, such as HIV-1 and malaria. In this study, we asked whether ZIKV infection during pregnancy impairs transplacental transfer of IgG. We enrolled pregnant women with fever or rash in a prospective cohort in Vitoria, Brazil during the recent ZIKV epidemic. ZIKV and dengue virus (DENV)-specific IgG, ZIKV and DENV neutralizing antibodies, and routine vaccine antigenspecific IgG were measured in maternal samples collected around delivery and 20 paired cord blood samples. We concluded that 8 of these mothers were infected with ZIKV during pregnancy and 12 were ZIKV-uninfected. The magnitude of flavivirus-specific IgG, neutralizing antibody, and vaccine-elicited IgG were highly correlated between maternal plasma and infant cord blood in both ZIKV-infected and -uninfected mother-infant pairs. Moreover, there was no difference in the magnitude of plasma flavivirus-specific IgG levels between mothers and infants regardless of ZIKV infection status. Our data suggests that maternal ZIKV infection during pregnancy does not impair the efficiency of placental transfer of flavivirus-specific, functional, and vaccine-elicited IgG. These findings have implications for the neonatal outomes of maternal ZIKV infection and optimal administration of antibody-based ZIKV vaccines and therapeutics

Diversity of Zoanthids (Anthozoa: Hexacorallia) on Hawaiian Seamounts: Description of the Hawaiian Gold Coral and Additional Zoanthids

The Hawaiian gold coral has a history of exploitation from the deep slopes and seamounts of the Hawaiian Islands as one of the precious corals commercialised in the jewellery industry. Due to its peculiar characteristic of building a scleroproteic skeleton, this zoanthid has been referred as Gerardia sp. (a junior synonym of Savalia Nardo, 1844) but never formally described or examined by taxonomists despite its commercial interest. While collection of Hawaiian gold coral is now regulated, globally seamounts habitats are increasingly threatened by a variety of anthropogenic impacts. However, impact assessment studies and conservation measures cannot be taken without consistent knowledge of the biodiversity of such environments. Recently, multiple samples of octocoral-associated zoanthids were collected from the deep slopes of the islands and seamounts of the Hawaiian Archipelago. The molecular and morphological examination of these zoanthids revealed the presence of at least five different species including the gold coral. Among these only the gold coral appeared to create its own skeleton, two other species are simply using the octocoral as substrate, and the situation is not clear for the final two species. Phylogenetically, all these species appear related to zoanthids of the genus Savalia as well as to the octocoral-associated zoanthid Corallizoanthus tsukaharai, suggesting a common ancestor to all octocoral-associated zoanthids. The diversity of zoanthids described or observed during this study is comparable to levels of diversity found in shallow water tropical coral reefs. Such unexpected species diversity is symptomatic of the lack of biological exploration and taxonomic studies of the diversity of seamount hexacorals

Improving object segmentation by using EEG signals and rapid serial visual presentation

This paper extends our previous work on the potential of EEG-based brain computer interfaces to segment salient objects in images. The proposed system analyzes the Event Related Potentials (ERP) generated by the rapid serial visual presentation of windows on the image. The detection of the P300 signal allows estimating a saliency map of the image, which is used to seed a semi-supervised object segmentation algorithm. Thanks to the new contributions presented in this work, the average Jaccard index was improved from $0.47$ to $0.66$ when processed in our publicly available dataset of images, object masks and captured EEG signals. This work also studies alternative architectures to the original one, the impact of object occupation in each image window, and a more robust evaluation based on statistical analysis and a weighted F-score