Isolation of a 60 kDa protein with in vitro anticancer activity against human cancer cell lines from the purple fluid of the Persian Gulf sea hare, Aplysia dactylomela

Sea hares have greatly attracted the interest of all those investigating chemical defense substances. Most of these substances are low molecular weight compounds derived from algal diets. In vitroanticancer effect of a 60 kDa protein isolated from the purple fluid of Aplysia dactylomela on four human cancer cell lines was investigated in this study. A 60 kDa protein was purified from secreted purple fluid of A. dactylomela, a sea hare from Persian Gulf. The protein purification procedure consisted basically of ammonium sulfate precipitation, ion exchange chromatography using DEAE– Sepharose and ultra-filtration method. In vitro antiproliferative and cytotoxic activity of the protein ofinterest were evaluated on L929, K562, HL60 and NB4 human cancer cell lines. The antiproliferative and cytotxic effects of 60 kDa protein on human cancer cell lines were measured by MTT assay. Resultsshowed that the 60 kDa protein of the purple fluid of A. dactylomela exhibited the antiproliferative effect on human cancer cell lines, especially on NB4 cell line. It was maximally active at 0.5 – 1.5 ìg/ml on NB4 cell line. Interestingly, the protein did not show significant cytotoxic effects

Towards an aesthetics of theatre technology

This thesis establishes groundwork for producing an aesthetic language for theatre technology by creating and testing a model for looking at theatre technologies in a critical manner. This model has several functions: Firstly it identifies theatre technology as something which can have a specific or a psycho-plastic scenographic effect. Through processes of re-invigoration and diversification the model allows a device to be regarded in its own context while historiologically allowing for precedent technologies to be acknowledged and compared. Lastly, because the model is ouroboric, self-consuming, it accounts for theatre technologies to be able to interpolate (and be interpolated by) other technologies whilst maintaining its own aesthetic integrity. This allows a critic to treat technology as a text rather than as a medium, and therefore enables it to be closely "read" as a text of the stage affording the technology a content of its own. Through problematising this model against theories of media and remediation, the thesis observes that the common critical position in theatre and performance studies is to treat theatre technology merely as a theatrical technē -- a tool or craft of the art. The arguments presented in the thesis reposition theatre technology from the position of craft to a position of art -- as alētheia, an artistic truth revealed through poiētic means. In the repositioning of attitudes towards technology, and by identifying theatre technologies as separate alētheuein, this thesis is then able to investigate theatre technologies aesthetically. Examining the contexts of technologies through the ouroboric model, and then critically studying their content, usage and meaning textually, this thesis is able to take a theatrical technological effect and begin to identify its affect. It posits that technology as an art in its own right can be aesthetically criticised and awarded meaning of equal weight to other elements of performance and theatre art.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Purification and characterization of extracellular α-amylase from a thermophilic Anoxybacillus thermarum A4 strain

Adiguzel, Ahmet/0000-0001-8848-6647WOS: 000396245100008alpha-Amylase from Anoxybacillus thermarum A4 was purified using ammonium sulphate precipitation and Sephadex G-100 gel filtration chromatography, with 29.8-fold purification and 74.6% yield. A4 amylase showed best performance for soluble potato starch hydrolysis at 70 degrees C and pH 5.5-10.5. A4 amylase was extremely stable at +4 degrees C, and the enzyme retained over 65% of its original alpha-amylase activity at 70 degrees C and 43% at 90 degrees C. the enzyme's Km values for soluble starch, amylopectin and amylose substrates were obtained as 0.9, 1.3 and 0.5 mg/mL, respectively. EDTA, Hg2+, B4O72-, OH-, CN-, and urea exhibited different inhibition effects; their IC50 values were identified as 8.0, 5.75, 16.5, 15.2, 8.2 and 10.9 mM, respectively. A4 amylase exhibited extreme stability toward some surfactants and perfect match for a wide variety of commercial solid and liquid detergents at 55 degrees C. So, it may be considered to be potential applications for detergent and other industrial uses