159 research outputs found
Peri-Vesical Fat Interposition Flap Reinforcement in High Vesico-Vaginal Fistulas
Background and Aim: The urinary bladder becomes small, contracted and is associated with excess pelvic fat in long standing cases of vesico-vaginal fistulas (VVFs). The aim of this new technique was to use this excess pelvic fat for harvesting an interposition flap. Materials and Methods: An interposition flap of peri-vesical fat was raised from the anterior, superior and posterior surfaces of the urinary bladder and was interposed between the right angle closed vaginal vault and the urinary bladder to strengthen the repair. This technique was used in two patients of VVFs. Results: Both the patients had successful outcome and were able to retain sufficient quantity of urine at 3 months follow-up. Conclusions: Peri-vesical fat flap proved an effective
interposition flap in the repairs of VVFs in selected cases.
Key words: Interposition flap, peri-vesical fat flap, vesicovaginal fistula
Gravitational detection of a low-mass dark satellite at cosmological distance
The mass-function of dwarf satellite galaxies that are observed around Local
Group galaxies substantially differs from simulations based on cold dark
matter: the simulations predict many more dwarf galaxies than are seen. The
Local Group, however, may be anomalous in this regard. A massive dark satellite
in an early-type lens galaxy at z = 0.222 was recently found using a new method
based on gravitational lensing, suggesting that the mass fraction contained in
substructure could be higher than is predicted from simulations. The lack of
very low mass detections, however, prohibited any constraint on their mass
function. Here we report the presence of a 1.9 +/- 0.1 x 10^8 M_sun dark
satellite in the Einstein-ring system JVAS B1938+666 at z = 0.881, where M_sun
denotes solar mass. This satellite galaxy has a mass similar to the Sagittarius
galaxy, which is a satellite of the Milky Way. We determine the logarithmic
slope of the mass function for substructure beyond the local Universe to be
alpha = 1.1^+0.6_-0.4, with an average mass-fraction of f = 3.3^+3.6_-1.8 %, by
combining data on both of these recently discovered galaxies. Our results are
consistent with the predictions from cold dark matter simulations at the 95 per
cent confidence level, and therefore agree with the view that galaxies formed
hierarchically in a Universe composed of cold dark matter.Comment: 25 pages, 7 figures, accepted for publication in Nature (19 January
2012
Clumps and streams in the local dark matter distribution
In cold dark matter cosmological models, structures form and grow by merging
of smaller units. Numerical simulations have shown that such merging is
incomplete; the inner cores of halos survive and orbit as "subhalos" within
their hosts. Here we report a simulation that resolves such substructure even
in the very inner regions of the Galactic halo. We find hundreds of very
concentrated dark matter clumps surviving near the solar circle, as well as
numerous cold streams. The simulation reveals the fractal nature of dark matter
clustering: Isolated halos and subhalos contain the same relative amount of
substructure and both have cuspy inner density profiles. The inner mass and
phase-space densities of subhalos match those of recently discovered faint,
dark matter-dominated dwarf satellite galaxies and the overall amount of
substructure can explain the anomalous flux ratios seen in strong gravitational
lenses. Subhalos boost gamma-ray production from dark matter annihilation, by
factors of 4-15, relative to smooth galactic models. Local cosmic ray
production is also enhanced, typically by a factor 1.4, but by more than a
factor of ten in one percent of locations lying sufficiently close to a large
subhalo. These estimates assume that gravitational effects of baryons on dark
matter substructure are small.Comment: 14 pages, 5 figures, to appear in Nature, includes supplementary
information. Full version of Figure 1 available at
http://www.ucolick.org/~diemand/vl2/fig1.pn
A New Era in the Quest for Dark Matter
There is a growing sense of `crisis' in the dark matter community, due to the
absence of evidence for the most popular candidates such as weakly interacting
massive particles, axions, and sterile neutrinos, despite the enormous effort
that has gone into searching for these particles. Here, we discuss what we have
learned about the nature of dark matter from past experiments, and the
implications for planned dark matter searches in the next decade. We argue that
diversifying the experimental effort, incorporating astronomical surveys and
gravitational wave observations, is our best hope to make progress on the dark
matter problem.Comment: Published in Nature, online on 04 Oct 2018. 13 pages, 1 figur
Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging
Time-lapse imaging of multiple labels is challenging for biological imaging as noise, photobleaching and phototoxicity compromise signal quality, while throughput can be limited by processing time. Here, we report software called Hyper-Spectral Phasors (HySP) for denoising and unmixing multiple spectrally overlapping fluorophores in a low signal-to-noise regime with fast analysis. We show that HySP enables unmixing of seven signals in time-lapse imaging of living zebrafish embryos
The Hubble Constant
I review the current state of determinations of the Hubble constant, which
gives the length scale of the Universe by relating the expansion velocity of
objects to their distance. There are two broad categories of measurements. The
first uses individual astrophysical objects which have some property that
allows their intrinsic luminosity or size to be determined, or allows the
determination of their distance by geometric means. The second category
comprises the use of all-sky cosmic microwave background, or correlations
between large samples of galaxies, to determine information about the geometry
of the Universe and hence the Hubble constant, typically in a combination with
other cosmological parameters. Many, but not all, object-based measurements
give values of around 72-74km/s/Mpc , with typical errors of 2-3km/s/Mpc.
This is in mild discrepancy with CMB-based measurements, in particular those
from the Planck satellite, which give values of 67-68km/s/Mpc and typical
errors of 1-2km/s/Mpc. The size of the remaining systematics indicate that
accuracy rather than precision is the remaining problem in a good determination
of the Hubble constant. Whether a discrepancy exists, and whether new physics
is needed to resolve it, depends on details of the systematics of the
object-based methods, and also on the assumptions about other cosmological
parameters and which datasets are combined in the case of the all-sky methods.Comment: Extensively revised and updated since the 2007 version: accepted by
Living Reviews in Relativity as a major (2014) update of LRR 10, 4, 200
Fibroblast growth factor 23 is associated with proteinuria and smoking in chronic kidney disease: An analysis of the MASTERPLAN cohort
Contains fulltext :
107913.pdf (postprint version ) (Open Access)BACKGROUND: Fibroblast growth factor 23 (FGF23) has emerged as a risk factor for cardiovascular disease and mortality throughout all stages of chronic kidney disease (CKD), independent from established risk factors and markers of mineral homeostasis. The relation of FGF23 with other renal and non-renal cardiovascular risk factors is not well established. METHODS: Using stored samples, plasma FGF23 was determined in 604 patients with moderate to severe kidney disease that participated in the MASTERPLAN study (ISRCTN73187232). The association of FGF23 with demographic and clinical parameters was evaluated using multivariable regression models. RESULTS: Mean age in the study population was 60 years and eGFR was 37 (+/- 14) ml/min/1.73 m(2). Median proteinuria was 0.3 g/24 hours [IQR 0.1-0.9]. FGF23 level was 116 RU/ml [67-203] median and IQR. Using multivariable analysis the natural logarithm of FGF23 was positively associated with history of cardiovascular disease (B = 0.224 RU/ml; p = 0.002), presence of diabetes (B = 0.159 RU/ml; p = 0.035), smoking (B = 0.313 RU/ml; p < 0.001), phosphate level (B = 0.297 per mmol/l; p = 0.0024), lnPTH (B = 0.244 per pmol/l; p < 0.001) and proteinuria (B = 0.064 per gram/24 hrs; p = 0.002) and negatively associated with eGFR (B = -0.022 per ml/min/1.73 m(2); p < 0.001). CONCLUSIONS: Our study demonstrates that in patients with CKD, FGF23 is related to proteinuria and smoking. We confirm the relation between FGF23 and other cardiovascular risk factors
Neocentromeres Form Efficiently at Multiple Possible Loci in Candida albicans
Centromeres are critically important for chromosome stability and integrity. Most eukaryotes have regional centromeres that include long tracts of repetitive DNA packaged into pericentric heterochromatin. Neocentromeres, new sites of functional kinetochore assembly, can form at ectopic loci because no DNA sequence is strictly required for assembly of a functional kinetochore. In humans, neocentromeres often arise in cells with gross chromosome rearrangements that rescue an acentric chromosome. Here, we studied the properties of centromeres in Candida albicans, the most prevalent fungal pathogen of humans, which has small regional centromeres that lack pericentric heterochromatin. We functionally delimited centromere DNA on Chromosome 5 (CEN5) and then replaced the entire region with the counter-selectable URA3 gene or other marker genes. All of the resulting cen5Ξ::URA3 transformants stably retained both copies of Chr5, indicating that a functional neocentromere had assembled efficiently on the homolog lacking CEN5 DNA. Strains selected to maintain only the cen5Ξ::URA3 homolog and no wild-type Chr5 homolog also grew well, indicating that neocentromere function is independent of the presence of any wild-type CEN5 DNA. Two classes of neocentromere (neoCEN) strains were distinguishable: βproximal neoCENβ and βdistal neoCENβ strains. Neocentromeres in the distal neoCEN strains formed at loci about 200β450 kb from cen5Ξ::URA3 on either chromosome arm, as detected by massively parallel sequencing of DNA isolated by CENP-ACse4p chromatin immunoprecipitation (ChIP). In the proximal neoCEN strains, the neocentromeres formed directly adjacent to cen5Ξ::URA3 and moved onto the URA3 DNA, resulting in silencing of its expression. Functional neocentromeres form efficiently at several possible loci that share properties of low gene density and flanking repeated DNA sequences. Subsequently, neocentromeres can move locally, which can be detected by silencing of an adjacent URA3 gene, or can relocate to entirely different regions of the chromosome. The ability to select for neocentromere formation and movement in C. albicans permits mechanistic analysis of the assembly and maintenance of a regional centromere
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