Antibodies to glycans dominate the host response to schistosome larvae and eggs: Is their role protective or subversive?

Multiple exposures of chimpanzees to the radiation-attenuated schistosome vaccine provoked a strong parasite-specific cellular and humoral immune response. Specific IgM and IgG were directed mainly against glycans on antigens released by cercariae; these were also cross-reactive with soluble antigens from larvae, adult worms, and eggs. Egg deposition was the major antigenic stimulus after challenge of vaccinated and control chimpanzees with normal parasites, eliciting strong antiglycan responses to egg secretions. Glycan epitopes recognized included LacdiNAc, fucosylated LacdiNAc, LewisX (weakly), and those on keyhole limpet hemocyanin. Antibodies to peptide epitopes became prominent only during the chronic phase of infection, as glycan-specific IgM and IgG decreased. Because of their intensity and cross-reactivity, the antiglycan responses resulting from infection could be a smoke screen to subvert the immune system away from more vulnerable larval peptide epitopes. Their occurrence in humans might explain the long time required for antischistosome immunity to build up after infection

Spectrum shape-analysis techniques applied to the Hanford Tank Farms spectral gamma logs

Gamma-ray spectra acquired with high-energy resolution by the spectral gamma logging systems (SGLSs) at the U.S. Department of Energy Hanford Tank Farms, Richland, Washington, are being analyzed for spectral shape characteristics. These spectral shapes, together with a conventional peak-area analysis, enable an analyst not only to identify the gamma-emitting species but also to determine in many instances its spatial distribution around a borehole and to identify the presence of the bremsstrahlung-producing contaminant {sup 90}Sr. The analysis relies primarily on the results of computer simulations of gamma spectra from the predominant radionuclide {sup 137}Cs for various spatial distributions. This log analysis methodology has evolved through an examination of spectral features from spectral logs taken at the SX, BY, and U Tank Farms at the Hanford Site. Initial results determined with this technique show it is possible, in most cases, to distinguish between concentrations of {sup 137}Cs. Work is continuing by experimentally measuring shape factors, incorporating spectrum shape processing in routine log analysis, and extending the techniques to additional radionuclides

Studies of oxidative stress in cellular systems The interaction of monocytes and erythrocytes

Abstract1H spin echo NMR spectroscopy is used to follow the interaction of intact and viable erythrocytes and monocytes obtained from different sources in mixed cultures. After a lag time (270 min) erythrocyte glutathione is observed to become more oxidised. This result is believed to occur as a consequence of monocyte activation generating hydrogen peroxide or hypochlorous acid, which is targeted at the erythrocyte. The red cell in turn employs its sulphydryl system as an anti-oxidant defence

Chiral Symmetry in Light-front QCD

The definition of chiral transformations in light-front field theory is very different from the conventional form in equal-time formalism. We study the consistency of chiral transformations and chiral symmetry in light-front QCD and derive a complete new light-front axial-vector current for QCD. The breaking of chiral symmetry in light-front QCD is only associated with helicity flip interaction between quarks and gluons. Remarkably, the new axial-vector current does not contain the pion pole part so that the associate chiral charge smoothly describes pion transitions for various hadronic processes.Comment: 15 pages, no figure, JHEP style, added reference and corrected typos and some changed conten

Ethnicity data collection in the UK: the healthcare professional's perspective

The collection of ethnicity data has been demonstrated to be important in healthcare. However, despite recent efforts by the UK government, it remains incomplete and unvalidated. In order to be able to assess inequalities and target resources appropriately, it is essential to have complete and accurate data. This paper examines the reasons for the gaps in ethnicity data based on the perceptions and experiences of the healthcare professionals who are charged with collecting these data. A questionnaire was used to assess perceptions of ethnicity data collection, including any barriers encountered as well as the perceived importance of collecting these data. Respondents were asked whether routine ethnicity data collection was limited to specific disease areas, and approximately what proportion was complete in these areas. There were also questions concerning preferred methods of collection (e.g. self-report). The questionnaire was completed by 30 respondents, who included healthcare managers, clinicians, nurses and other staff working in the healthcare setting. The findings confirmed that the collection of patients’ ethnicity data is deemed important by the healthcare professionals, but showed that there remains uncertainty and unease as to how best to collect these data or how to explain to patients how the data will be used. The majority of healthcare professionals agreed that it was important to record patients’ ethnicity, but no clear rationale was given to staff about the use of these data, and no training was provided on the best way to collect the data.</p

Borehole breakout analysis: results from the AND-2A Well

To define the present-day stress field in the upper crust and to understand the recent tectonic activity in Antarctica, a study of breakout measurements along AND-2A well was performed. The borehole breakout is an important indicator of horizontal stress orientation and occurs when the stresses around the borehole exceed that required to cause compressive failure of the borehole wall (Bell and Gough, 1979; Zoback et al., 1985, Bell, 1990). The enlargement of the wellbore is caused by the development of intersecting conjugate shear planes that cause pieces of the borehole wall to spall off. Around a vertical borehole, stress concentration is greatest in the direction of the minimum horizontal stress (Shmin), hence, the long axes of borehole breakouts are oriented approximately perpendicular to the maximum horizontal stress orientation (SHmax). The orientation of breakouts along the AND-2A well was measured using acoustic (BHTV) and mechanical (Four-Arm Caliper) tools. Borehole televiewer (BHTV) provides an acoustic "image" of the borehole wall (360 degree coverage) and gives detailed information for investigation of fractures and stress analysis. The four-arm caliper is the oldest technique for borehole breakout identification and it is included in routine dipmeter logs. A quality value has been assigned to the well results in agreement with the World Stress Map quality ranking scheme (Zoback, 1992; Heidback et al., 2010) based mainly on the number, accuracy, and length of breakout measurements. The result is presented as rose diagram of the breakout directions where the length of each peak is proportional to the frequency and the width to the variance of its gaussian curve. We have analyzed the following curves to recognize the breakout: the azimuth of Pad 1 (P1az), the drift azimuth (HAZI), the two calipers with respect to the bit size (BZ) curve and the curve relative to the deviation of the well. The AND-2A Four-Arm Caliper data cover a depth interval between 637 down to 997 mbsl, that corresponds to 360 m of logged interval. We have distinguished breakouts and some washouts only in the interval from 753 to 825 mbsl. From borehole televiewer images, we have data from 398 mbsl down to 1136 mbsl. The BHTV worked well showing a lot of interesting features such as many bedding, lamination and fractures (natural and induced) but poor breakouts. The rare breakouts have also a small size (called protobreakouts) but they are consistent with induced features. Considering the breakout result from caliper and BHTV, the AND-2A borehole is unfortunately classified as D quality. This means that to obtain a reliable active stress field of the area it is necessary to compare this result with other available data

Field Measurements of Terrestrial and Martian Dust Devils

Surface-based measurements of terrestrial and martian dust devils/convective vortices provided from mobile and stationary platforms are discussed. Imaging of terrestrial dust devils has quantified their rotational and vertical wind speeds, translation speeds, dimensions, dust load, and frequency of occurrence. Imaging of martian dust devils has provided translation speeds and constraints on dimensions, but only limited constraints on vertical motion within a vortex. The longer mission durations on Mars afforded by long operating robotic landers and rovers have provided statistical quantification of vortex occurrence (time-of-sol, and recently seasonal) that has until recently not been a primary outcome of more temporally limited terrestrial dust devil measurement campaigns. Terrestrial measurement campaigns have included a more extensive range of measured vortex parameters (pressure, wind, morphology, etc.) than have martian opportunities, with electric field and direct measure of dust abundance not yet obtained on Mars. No martian robotic mission has yet provided contemporaneous high frequency wind and pressure measurements. Comparison of measured terrestrial and martian dust devil characteristics suggests that martian dust devils are larger and possess faster maximum rotational wind speeds, that the absolute magnitude of the pressure deficit within a terrestrial dust devil is an order of magnitude greater than a martian dust devil, and that the time-of-day variation in vortex frequency is similar. Recent terrestrial investigations have demonstrated the presence of diagnostic dust devil signals within seismic and infrasound measurements; an upcoming Mars robotic mission will obtain similar measurement types

Photovoltaic characterisation of GaAsBi/GaAs multiple quantum well devices

A series of strained GaAsBi/GaAs multiple quantum well diodes are characterised to assess the potential of GaAsBi for photovoltaic applications. The devices are compared with strained and strain-balanced InGaAs based devices. The dark currents of the GaAsBi based devices are around 20 times higher than those of the InGaAs based devices. The GaAsBi devices that have undergone significant strain relaxation have dark currents that are a further 10–20 times higher. Quantum efficiency measurements show the GaAsBi devices have a lower energy absorption edge and stronger absorption than the strained InGaAs devices. These measurements also indicate incomplete carrier extraction from the GaAsBi based devices at short circuit, despite the devices having a relatively low background doping. This is attributed to hole trapping within the quantum wells, due to the large valence band offset of GaAsBi

Waveguide surface plasmon resonance biosensor for the aqueous environment

We report the fabrication and performance of gold coated waveguide surface plasmon resonance biosensors. Biotin-avidin binding reactions at the sensor surface were observed. The output power of the sensor showed a decrease of 32% on binding a dual layer of biotin-avidin