The response of lateral buds of Pisum sativum to decapitation and selective bud excision

Seedlings of Pisum sativum L. cv. Onward exhibiting complete apical dominance were decapitated above the fourth node and one or more of the four inhibited lateral buds then excised. Lateral bud length and mass were measured as an indicator of the degree of inhibition experienced by each bud as well as the influence of one growing bud on another in the whole shoot system. The results are discussed in relation to theoretical gradients of nutrients and growth regulators in the plant

Future Prospects: Deep Imaging of Galaxy Outskirts using Telescopes Large and Small

The Universe is almost totally unexplored at low surface brightness levels. In spite of great progress in the construction of large telescopes and improvements in the sensitivity of detectors, the limiting surface brightness of imaging observations has remained static for about forty years. Recent technical advances have at last begun to erode the barriers preventing progress. In this Chapter we describe the technical challenges to low surface brightness imaging, describe some solutions, and highlight some relevant observations that have been undertaken recently with both large and small telescopes. Our main focus will be on discoveries made with the Dragonfly Telephoto Array (Dragonfly), which is a new telescope concept designed to probe the Universe down to hitherto unprecedented low surface brightness levels. We conclude by arguing that these discoveries are probably only scratching the surface of interesting phenomena that are observable when the Universe is explored at low surface brightness levels.Comment: 27 pages, 10 figures, Invited review, Book chapter in "Outskirts of Galaxies", Eds. J. H. Knapen, J. C. Lee and A. Gil de Paz, Astrophysics and Space Science Library, Springer, in pres

Cryptococcus neoformans escape From Dictyostelium amoeba by both WASH-mediated constitutive exocytosis and vomocytosis

Cryptococcus neoformans is an environmental yeast that can cause opportunistic infections in humans. As infecting animals does not form part of its normal life-cycle, it has been proposed that the virulence traits that allow cryptococci to resist immune cells were selected through interactions with environmental phagocytes such as amoebae. Here, we investigate the interactions between C. neoformans and the social amoeba Dictyostelium discoideum. We show that like macrophages, D. discoideum is unable to kill C. neoformans upon phagocytosis. Despite this, we find that the yeast pass through the amoebae with an apparently normal phagocytic transit and are released alive by constitutive exocytosis after ~80 min. This is the canonical pathway in amoebae, used to dispose of indigestible material after nutrient extraction. Surprisingly however, we show that upon either genetic or pharmacological blockage of constitutive exocytosis, C. neoformans still escape from D. discoideum by a secondary mechanism. We demonstrate that constitutive exocytosis-independent egress is stochastic and actin-independent. This strongly resembles the non-lytic release of cryptococci by vomocytosis from macrophages, which do not perform constitutive exocytosis and normally retain phagocytosed material. Our data indicate that vomocytosis is functionally redundant for escape from amoebae, which thus may not be the primary driver for its evolutionary selection. Nonetheless, we show that vomocytosis of C. neoformans is mechanistically conserved in hosts ranging from amoebae to man, providing new avenues to understand this poorly-understood but important virulence mechanism

Effects of Strong Magnetic Fields in Strange Baryonic Matter

We investigate the effects of very strong magnetic fields upon the equation of state of dense bayonic matter in which hyperons are present. In the presence of a magnetic field, the equation of state above nuclear density is significantly affected both by Landau quantization and magnetic moment interactions, but only for field strengths $B>5\times10^{18}$ G. The former tends to soften the EOS and increase proton and lepton abundances, while the latter produces an overall stiffening of the EOS. Each results in a supression of hyperons relative to the field-free case. The structure of a neutron star is, however, primarily determined by the magnetic field stress. We utilize existing general relativistic magneto-hydrostatic calculations to demonstrate that maximum average fields within a stable neutron are limited to values $B\le 1-3 \times10^{18}$ G. This is not large enough to significantly influence particle compositions or the matter pressure, unless fluctuations dominate the average field strengths in the interior or configurations with significantly larger field gradients are considered.Comment: 12 pages, 3 figures. To be submitted to Phys. Lett.

A pearl on SAT solving in Prolog

A succinct SAT solver is presented that exploits the control provided by delay declarations to implement watched literals and unit propagation. Despite its brevity the solver is surprisingly powerful and its elegant use of Prolog constructs is presented as a programming pearl

Are Antiprotons Forever?

Up to one million antiprotons from a single LEAR spill have been captured in a large Penning trap. Surprisingly, when the antiprotons are cooled to energies significantly below 1 eV, the annihilation rate falls below background. Thus, very long storage times for antiprotons have been demonstrated in the trap, even at the compromised vacuum conditions imposed by the experimental set up. The significance for future ultra-low energy experiments, including portable antiproton traps, is discussed.Comment: 12 pages, latex; 4 figures, uufiled. Slightly expanded discussion of expected energy dependence of annihilation cross section and rate, and of estimates of trap pressure, plus minor text improvement

Biodiesel feedstock determines exhaust toxicity in 20% biodiesel: 80% mineral diesel blends

To address climate change concerns, and reduce the carbon footprint caused by fossil fuel use, it is likely that blend ratios of renewable biodiesel with commercial mineral diesel fuel will steadily increase, resulting in biodiesel use becoming more widespread. Exhaust toxicity of unblended biodiesels changes depending on feedstock type, however the effect of feedstock on blended fuels is less well known. The aim of this study was to assess the impact of biodiesel feedstock on exhaust toxicity of 20% blended biodiesel fuels (B20). Primary human airway epithelial cells were exposed to exhaust diluted 1/15 with air from an engine running on conventional ultra-low sulfur diesel (ULSD) or 20% blends of soy, canola, waste cooking oil (WCO), tallow, palm or cottonseed biodiesel in diesel. Physico-chemical exhaust properties were compared between fuels and the post-exposure effect of exhaust on cellular viability and media release was assessed 24 h later. Exhaust properties changed significantly between all fuels with cottonseed B20 being the most different to both ULSD and its respective unblended biodiesel. Exposure to palm B20 resulted in significantly decreased cellular viability (96.3 ± 1.7%; p < 0.01) whereas exposure to soy B20 generated the greatest number of changes in mediator release (including IL-6, IL-8 and TNF-α, p < 0.05) when compared to air exposed controls, with palm B20 and tallow B20 closely following. In contrast, canola B20 and WCO B20 were the least toxic with only mediators G-CSF and TNF-α being significantly increased. Therefore, exposure to palm B20, soy B20 and tallow B20 were found to be the most toxic and exposure to canola B20 and WCO B20 the least. The top three most toxic and the bottom three least toxic B20 fuels are consistent with their unblended counterparts, suggesting that feedstock type greatly impacts exhaust toxicity, even when biodiesel only comprises 20% of the fuel