Physiological Evidence for Isopotential Tunneling in the Electron Transport Chain of Methane-Producing Archaea

Many, but not all, organisms use quinones to conserve energy in their electron transport chains. Fermentative bacteria and methane-producing archaea (methanogens) do not produce quinones but have devised other ways to generate ATP. Methanophenazine (MPh) is a unique membrane electron carrier found in Methanosarcina species that plays the same role as quinones in the electron transport chain. To extend the analogy between quinones and MPh, we compared the MPh pool sizes between two well-studied Methanosarcina species, Methanosarcina acetivorans C2A and Methanosarcina barkeri Fusaro, to the quinone pool size in the bacterium Escherichia coli. We found the quantity of MPh per cell increases as cultures transition from exponential growth to stationary phase, and absolute quantities of MPh were 3-fold higher in M. acetivorans than in M. barkeri. The concentration of MPh suggests the cell membrane of M. acetivorans, but not of M. barkeri, is electrically quantized as if it were a single conductive metal sheet and near optimal for rate of electron transport. Similarly, stationary (but not exponentially growing) E. coli cells also have electrically quantized membranes on the basis of quinone content. Consistent with our hypothesis, we demonstrated that the exogenous addition of phenazine increases the growth rate of M. barkeri three times that of M. acetivorans. Our work suggests electron flux through MPh is naturally higher in M. acetivorans than in M. barkeri and that hydrogen cycling is less efficient at conserving energy than scalar proton translocation using MPh

A Review of the Role of Melatonin in Irritable Bowel Syndrome

Irritable bowel syndrome (IBS) is a troubling disease experienced worldwide. The presentation of symptoms varies from patient to patient, and current prescription treatments can be inadequate in resolving symptoms. This article explores the available scientific literature supporting the use of melatonin in alleviating IBS symptoms

Distribution of Spectral Characteristics and the Cosmological Evolution of GRBs

We investigate the cosmological evolution of GRBs, using the total gamma ray fluence as a measure of the burst strength. This involves an understanding of the distributions of the spectral parameters of GRBs as well as the total fluence distribution - both of which are subject to detector selection effects. We present new non-parametric statistical techniques to account for these effects, and use these methods to estimate the true distribution of the peak of the nu F_nu spectrum, E_p, from the raw distribution. The distributions are obtained from four channel data and therefore are rough estimates. Here, we emphasize the methods and present qualitative results. Given its spectral parameters, we then calculate the total fluence for each burst, and compute its cumulative and differential distributions. We use these distributions to estimate the cosmological rate evolution of GRBs, for three cosmological models. Our two main conclusions are the following: 1) Given our estimates of the spectral parameters, we find that there may exist a significant population of high E_p bursts that are not detected by BATSE, 2) We find a GRB co-moving rate density quite different from that of other extragalactic objects; in particular, it is different from the recently determined star formation rate.Comment: 20 pages, including 10 postscript figures. Submitted to Ap

Sparse Codes for Speech Predict Spectrotemporal Receptive Fields in the Inferior Colliculus

We have developed a sparse mathematical representation of speech that minimizes the number of active model neurons needed to represent typical speech sounds. The model learns several well-known acoustic features of speech such as harmonic stacks, formants, onsets and terminations, but we also find more exotic structures in the spectrogram representation of sound such as localized checkerboard patterns and frequency-modulated excitatory subregions flanked by suppressive sidebands. Moreover, several of these novel features resemble neuronal receptive fields reported in the Inferior Colliculus (IC), as well as auditory thalamus and cortex, and our model neurons exhibit the same tradeoff in spectrotemporal resolution as has been observed in IC. To our knowledge, this is the first demonstration that receptive fields of neurons in the ascending mammalian auditory pathway beyond the auditory nerve can be predicted based on coding principles and the statistical properties of recorded sounds.Comment: For Supporting Information, see PLoS website: http://www.ploscompbiol.org/article/info%3Adoi%2F10.1371%2Fjournal.pcbi.100259

Genetic Toolkit for Assessment and Prediction of Population-Level Impacts of Bridge Construction on Birds

Recent studies have highlighted alarming rates of declines in bird populations across the country. The State of California is home to over 650 resident and migrant avian species. Legislation for protecting these species has existed for over a century now, yet tools for identifying populations and understanding seasonal movement remain limited. Recently, genetic and genomic tools have provided a method for understanding population structure, allowing for more informed delineation of management units. The goal of this project was to create a genetic toolkit for identifying breeding populations and assigning individuals to those populations. Ultimately, such tools could be used to assess population-level impacts when there are conflicts with birds at infrastructure construction sites. As a test case, we sequenced entire genomes for 40 individual Anna’s hummingbirds (Calypte anna) from across the state. Based on this initial data, we found low levels of differentiation between sampled locations, suggesting that C. anna in California are not subdivided into different population units. However, there was a weak signal of geography suggesting there may be localized genetic differences in a small proportion of the genome. Follow-up work will focus on a broader sampling across the state of California to clarify any possible population subdivision or geographical patterns of differentiation.View the NCST Project Webpag